Fukuoka Agriculture and Forestry Research Center

Fukuoka-shi, Japan

Fukuoka Agriculture and Forestry Research Center

Fukuoka-shi, Japan
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Teshiba M.,Fukuoka Agriculture and Forestry Research Center | Tabata J.,Japan National Agriculture and Food Research Organization
Applied Entomology and Zoology | Year: 2017

We previously reported that two parasitic wasps, Anagyrus sawadai Ishii and Leptomastix dactylopii Howard, are strongly attracted to (2,4,4-trimethyl-2-cyclohexenyl)methyl butyrate (cyclolavandulyl butyrate, CLB), a cyclization product of the sex pheromone of the Japanese mealybug, Planococcus kraunhiae (Kuwana). These wasps attacked more P. kraunhiae in the presence of CLB in our field experiments. In the present study, we showed that these CLB-attracted wasps parasitized and suppressed the mealybug population increase in field persimmon orchards, which would lead to reducing mealybug damage on commercial products. Although many attractants for natural enemies are reported, compounds such as CLB that suppress pest population growth in fields are scarce. Moreover, to the best of our knowledge, this is currently the only example to demonstrate that the “non-natural” enemy that does not typically attack the pest under natural conditions can be enrolled in biological control by using its attractant. © 2016, The Japanese Society of Applied Entomology and Zoology.


Sasaki K.,Japan National Agriculture and Food Research Organization | Motoyama M.,Japan National Agriculture and Food Research Organization | Tagawa Y.,Tottori Swine & Poultry Experimental Station | Akama K.,Tochigi Prefecture Livestock & Dairy Experimental Center | And 3 more authors.
Journal of Poultry Science | Year: 2017

The texture of jidori-niku (Japanese indigenous native chicken meat) was characterized and compared with those of Chunky broiler chicken meat. Experiment 1: A qualitative sensory test using jidori-niku and broiler breast (pectoralis major, PM), thigh (biceps femoris, BF) and sasami (deep pectoral) meat cooked to the end-point temperature 75℃ by steam-heating was administered to a trained sensory panel (n=16−17) for the selection of descriptive texture items from ISO5492 texture words. By the correspondence analysis, the characteristics of ‘chewiness,’ ‘hardness’ and ‘springiness’ were found to be different between jidori-niku and broiler: they likely characterize jidori-niku texture. Experiment 2: Texture characteristics in the three above-mentioned muscles in jidoriniku and broiler were compared quantitatively using the three above-mentioned texture items by the trained sensory panel. Sensory chewiness and hardness were the highest in the broiler PM and second highest in the jidori-niku BF, whereas sensory springiness was the highest in the jidori-niku BF. These results suggest that jidori-niku-like texture was characterized as a springy texture as compared to broiler meat. © 2017, Japan Poultry Science Association.


PubMed | Ehime University, Kazusa DNA Research Institute, Fukuoka Agriculture and Forestry Research Center, Kyushu University and Fukuoka Agriculture and Forestry Research Center Buzen Branch
Type: | Journal: Scientific reports | Year: 2017

With the aim of identifying sex determinants of fig, we generated the first draft genome sequence of fig and conducted the subsequent analyses. Linkage analysis with a high-density genetic map established by a restriction-site associated sequencing technique, and genome-wide association study followed by whole-genome resequencing analysis identified two missense mutations in RESPONSIVE-TO-ANTAGONIST1 (RAN1) orthologue encoding copper-transporting ATPase completely associated with sex phenotypes of investigated figs. This result suggests that RAN1 is a possible sex determinant candidate in the fig genome. The genomic resources and genetic findings obtained in this study can contribute to general understanding of Ficus species and provide an insight into figs and plants sex determination system.


Mori M.,Fukuoka Agriculture and Forestry Research Center | Hayashi T.,Fukuoka Agriculture and Forestry Research Center | Isozaki Y.,Fukuoka Agriculture and Forestry Research Center | Takenouchi N.,Japan National Agriculture and Food Research Organization | Sakatani M.,Japan National Agriculture and Food Research Organization
Journal of Reproduction and Development | Year: 2015

In this study, the effect of heat shock on frozen-thawed blastocysts was evaluated using in vitro-produced (IVP) bovine embryos. In experiment 1, the effects of 6 h of heat shock at 41.0 C on fresh blastocysts were evaluated. HSPA1A expression as a reflection of stress was increased by heat shock (P < 0.05), but the expressions of the quality markers IFNT and POU5F1 were not affected. In experiment 2, frozen-thawed blastocysts were incubated at 38.5 C for 6 h (cryo-con) or exposed to heat shock at 41.0 C for 6 h (cryo-HS). Then, blastocysts were cultured at 38.5 C until 48 h after thawing (both conditions). Cryo-HS blastocysts exhibited a decreased recovery rate: HSPA1A expression was dramatically increased compared with that in fresh or cryo-con blastocysts at 6 h, and IFNT expression was decreased compared with that in cryo-con blastocysts at 6 h (both P < 0.05). Cryo-con blastocysts at 6 h also exhibited higher HSPA1A expression than fresh blastocysts (P < 0.05). At 48 h after thawing, the number of hatched blastocysts and blastocyst diameter were lower in cryo-HS blastocysts (P < 0.05). Cryo-con blastocysts showed lower POU5F1 levels at 48 h than fresh, cryo-con or cryo-HS blastocysts at 6 h (P < 0.05), but their POU5F1 levels were not different from those of cryo-HS blastocysts at 48 h. These results indicated that application of heat shock to frozen-thawed blastocysts was highly damaging. The increase in damage by the interaction of freezing-thawing and heat shock might be one reason for the low conception rate in frozen-thawed embryo transfer in summer. © 2015 by the Society for Reproduction and Development.


PubMed | Animal Research Center, Japan National Agriculture and Food Research Organization, Fukuoka Agriculture and Forestry Research Center, Tochigi Prefecture Livestock & Dairy Experimental Center and Hokkaido University
Type: | Journal: Journal of the science of food and agriculture | Year: 2017

Over the past few decades, beef producers in Japan have improved marbling in their beef products. It was recently reported that marbling is not well correlated with palatability as rated by Japanese consumers. Here we sought to identify the consumer segments in Japanese that prefer sensory characteristics of beef other than high marbling.Three Wagyu beef, one Holstein beef, and two lean imported beef longissimus samples were subjected to a descriptive sensory test, physicochemical analysis, and consumer (n=307) preference test. According to consumer classification and external preference mapping, four consumer segments were identified as gradual high-fat likers, moderate-fat and distinctive-taste likers, Wagyu likers, and distinctive-texture likers. Although the major trend of Japanese consumers beef preference was marbling-liking, 16.9% of the consumers preferred beef samples that had moderate marbling and distinctive taste. The consumers attitudes expressed in a questionnaire survey was in good agreement with the preference for marbling among the moderate-fat and distinctive-taste likers.These results indicate that moderately marbled beef is a potent category in the Japanese beef market.


Mita T.,Japan National Agriculture and Food Research Organization | Nishimoto H.,Aichi Agricultural Research Center | Shimizu N.,Fukuoka Agriculture and Forestry Research Center | Mizutani N.,Japan National Agriculture and Food Research Organization
Applied Entomology and Zoology | Year: 2014

Trissolcus basalis (Wollaston), a cosmopolitan egg parasitoid of Nezara viridula (Linnaeus), was newly discovered in Central Honshu and Kyushu, Japan. The morphological characteristics useful to identify Tr. basalis from other species of Trissolcus occurring in Japan were indicated. Partial mitochondrial COI gene sequences of samples obtained from Aichi to Fukuoka Prefectures almost completely correspond to those obtained from overseas retrieved from the DDBJ/Genbank database. The sequence of Tr. basalis was different by 13–17 % from Trissolcus species compared and Telenomus turesis (Dalman). Further field research is needed to clarify the impact of Tr. basalis on local parasitoid guilds. © 2014, The Japanese Society of Applied Entomology and Zoology.


Odahara T.,Japan National Institute of Advanced Industrial Science and Technology | Odahara K.,Fukuoka Agriculture and Forestry Research Center
Protein Expression and Purification | Year: 2016

Mixtures of neutral salts and polyethylene glycol are used for various purposes in biological studies. Although the effects of each component of the mixtures are theoretically well investigated, comprehension of their integrated effects remains insufficient. In this work, their roles and effects as a precipitant were clarified by studying dependence of precipitation curves on salt concentration for integral membrane protein/detergent particles of different physicochemical properties. The dependence of precipitation curves was reasonably related to intermolecular interactions among relevant molecules such as protein, detergent and polyethylene glycol by considering their physicochemical properties. The obtained relationships are useful as basic information to learn the early stage of biological macromolecular associations. © 2015 Elsevier Inc. All rights reserved.


PubMed | Japan National Institute of Advanced Industrial Science and Technology and Fukuoka Agriculture and Forestry Research Center
Type: | Journal: Protein expression and purification | Year: 2016

Mixtures of neutral salts and polyethylene glycol are used for various purposes in biological studies. Although the effects of each component of the mixtures are theoretically well investigated, comprehension of their integrated effects remains insufficient. In this work, their roles and effects as a precipitant were clarified by studying dependence of precipitation curves on salt concentration for integral membrane protein/detergent particles of different physicochemical properties. The dependence of precipitation curves was reasonably related to intermolecular interactions among relevant molecules such as protein, detergent and polyethylene glycol by considering their physicochemical properties. The obtained relationships are useful as basic information to learn the early stage of biological macromolecular associations.


PubMed | Japan National Institute of Advanced Industrial Science and Technology and Fukuoka Agriculture and Forestry Research Center
Type: | Journal: Data in brief | Year: 2016

The data provide information in support of the research article, Intermolecular interactions at early stage of protein/detergent particle association induced by salt/polyethylene glycol mixtures [1]. The data regarding variation of absorption spectra is used as an indicator of the duration of Rp. viridis PRU and RC, Rb. sphaeroides RC and LH2, and Rb. capsulatus LH2 in the native state in the presence of NaCl/polyethylene glycol (PEG) mixture. The data about minimum concentrations of salt and PEG whose aqueous phases are mutually separated presents information on additional influence of Tris buffer and N-octyl--d-glucoside on the salt-PEG phase separation.


PubMed | Fukuoka Agriculture and Forestry Research Center
Type: Comparative Study | Journal: The Journal of reproduction and development | Year: 2015

In this study, the effect of heat shock on frozen-thawed blastocysts was evaluated using in vitro-produced (IVP) bovine embryos. In experiment 1, the effects of 6 h of heat shock at 41.0 C on fresh blastocysts were evaluated. HSPA1A expression as a reflection of stress was increased by heat shock (P < 0.05), but the expressions of the quality markers IFNT and POU5F1 were not affected. In experiment 2, frozen-thawed blastocysts were incubated at 38.5 C for 6 h (cryo-con) or exposed to heat shock at 41.0 C for 6 h (cryo-HS). Then, blastocysts were cultured at 38.5 C until 48 h after thawing (both conditions). Cryo-HS blastocysts exhibited a decreased recovery rate: HSPA1A expression was dramatically increased compared with that in fresh or cryo-con blastocysts at 6 h, and IFNT expression was decreased compared with that in cryo-con blastocysts at 6 h (both P < 0.05). Cryo-con blastocysts at 6 h also exhibited higher HSPA1A expression than fresh blastocysts (P < 0.05). At 48 h after thawing, the number of hatched blastocysts and blastocyst diameter were lower in cryo-HS blastocysts (P < 0.05). Cryo-con blastocysts showed lower POU5F1 levels at 48 h than fresh, cryo-con or cryo-HS blastocysts at 6 h (P < 0.05), but their POU5F1 levels were not different from those of cryo-HS blastocysts at 48 h. These results indicated that application of heat shock to frozen-thawed blastocysts was highly damaging. The increase in damage by the interaction of freezing-thawing and heat shock might be one reason for the low conception rate in frozen-thawed embryo transfer in summer.

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