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Kajihara S.,Kochi University | Hojo H.,Kochi University | Koyanagi M.,Kochi University | Tanaka M.,Kochi University | And 5 more authors.
Plant Pathology | Year: 2012

Pseudomonas cichorii strain SPC9018 harbours hrp genes that encode a type III secretion system. The hrp mutants have reduced virulence on lettuce and lose virulence on aubergine. Of the hrp genes, the B3 gene (B3) is located next to hrpW, encoding a harpin, and is predicted to encode a small (12kDa) acidic (pI=5·21) chaperone. In this study, involvement of the two genes in SPC9018 virulence was analysed. An hrpW-mutant (CWN1) and a B3-deleted mutant (ΔB3) of SPC9018 lacked virulence on aubergine and had reduced virulence on lettuce. Furthermore, both mutants also lost their ability to induce the hypersensitive response (HR) in tobacco leaves. The phenotypes were similar to those of the hrpL-deficient mutant (SPC9018-L). Transformation of CWN1 and ΔB3 with the plasmid containing hrpW and B3, respectively, originating from SPC9018 restored their virulence and ability to induce the HR in tobacco leaves. The population of CWN1 drastically decreased in aubergine following inoculation. In contrast, the population of ΔB3 was retained after inoculation, which was similar to that observed with SPC9018-L. RT-PCR analysis showed that hrpW and B3 are transcribed as a single transcript and its expression is dependent on HrpL. The two-hybrid analysis showed association of HrpW with the B3 protein. Therefore, HrpW can be implicated in the virulence of P. cichorii and its ability to induce the HR in tobacco, with some assistance from the B3 protein, probably acting as a chaperone. © 2011 The Authors. Plant Pathology © 2011 BSPP. Source

Kase Y.,Japan National Institute of Radiological Sciences | Kase Y.,Proton Therapy | Kanai T.,Gunma University | Sakama M.,Nihon University | And 4 more authors.
Journal of Radiation Research | Year: 2011

The RBE-weighted absorbed dose, called "biological dose," has been routinely used for carbon-ion treatment planning in Japan to formulate dose prescriptions for treatment protocols. This paper presents a microdosimetric approach to measuring the biological dose, which was redefined to be derived from microdosimetric quantities measured by a tissue-equivalent proportional counter (TEPC). The TEPC was calibrated in 60Co gamma rays to assure a traceability of the TEPC measurement to Japanese standards and to eliminate the discrepancies among matching counters. The absorbed doses measured by the TEPC were reasonably coincident with those measured by a reference ionization chamber. The RBE value was calculated from the microdosimetric spectrum on the basis of the microdosimetric kinetic model. The biological doses obtained by the TEPC were compared with those prescribed in the carbon-ion treatment planning system. We found that it was reasonable for the measured biological doses to decrease with depth around the rear SOBP region because of beam divergence, scattering effect, and fragmentation reaction. These results demonstrate that the TEPC can be an effective tool to assure the radiation quality in carbon-ion radiotherapy. Source

Hokushin Co. and Fukui Prefectural Government | Date: 2013-04-19

A method and a device for opening a fiber bundle, capable of performing a fluctuating operation, at a high speed, of pushing a part of a conveyed fiber bundle by a contact member into a stress state and then separating the contact member from the fiber bundle so as to temporarily relax the fiber bundle, and also capable of reducing damage to the fiber bundle. The device for opening a fiber bundle includes a conveying portion

The present invention has an object to provide a fiber-spreading method which can form a thin spread fiber sheet having a large and uniform spread width by uniformly distributing fibers of a fiber bundle having an increased number of fibers. Since a set of regions S

Honda Corporation and Fukui Prefectural Government | Date: 2014-12-15

A method for producing a molded article of a fiber-reinforced plastic include molding a fiber-resin mixture containing an inorganic reinforcing fiber and a thermosetting resin under heat and pressure. The elapsed time since the thermosetting resin reached a molding temperature at which a hardening reaction proceeds is measured, the loss angle of the thermosetting resin is measured in a dynamic viscoelasticity measurement, and the relationship between the elapsed time and the loss angle is evaluated to calculate the range of the elapsed time, within which the loss angle is 0.55 to 1.57 rad after the loss angle reached the maximum value, as the pressurization start range. The fiber-resin mixture is heated to the molding temperature and pressure application to the fiber-resin mixture is started within the pressurization start range, to obtain the molded article of the fiber-reinforced plastic.

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