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Sugiyama M.,Nagoya University | Hattori H.,Nagoya University | Inoue T.,Nagoya University | Wakita H.,Fujita Health University Nanakuri Sanatorium | And 2 more authors.
Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology | Year: 2014

Purpose: The aim of this study was to investigate the effect of stem cells from human exfoliated deciduous teeth (SHED) after permanent MCAO (pMCAO). Materials and methods: Adult male Sprague-Dawley rats were subjected to pMCAO. After pMCAO, SHED was transplanted into the brain. Motor function and infarct volume were evaluated. Neurogenesis and vasculogenesis were determined using immunochemical markers. Results: The SHED group had more positive signals for doublecortin, neurofilament, anti-neuronal nuclei (NeuN) and rat endothelial cell antigen-1 (RECA1) in the peri-infarct area than the PBS group. Migration of doublecortin-positive neural progenitor cells (NPCs) from subventricular zone (SVZ) to the peri-infarct area was observed on day 16. Transplanted SHED merged vascular endothelial growth factor (VEGF) and stromal cell-derived factor 1 (SDF-1) positive cells. Conclusion: SHED promoted migration and differentiation of the endogenous NPCs and induced vasculogenesis, and ameliorated ischemic brain injury after pMCAO in rats. © 2013 Asian AOMS, ASOMP, JSOP, JSOMS, JSOM, and JAMI. Source


Okano H.,Suzuka General Hospital | Nakano T.,Fujita Health University Nanakuri Sanatorium
Acta Hepatologica Japonica | Year: 2014

We compared the clinical features and laboratory data of 13 cases with acute hepatitis E and 61 cases with drug-induced liver injury (DILI) in our hospital. Out of 13 hepatitis E cases, 12 had no history of eating or undercooked raw meat/viscera and remained unknown for the origin and route of hepatitis E virus (HEV) infection. More than half of acute hepatitis E and DILI did not exhibit jaundice during the clinical course. All 13 cases of acute hepatitis E had a high score of ≥5 points by the DDW-J 2004 scale. Without HEV screening, eight cases of acute hepatitis E might have been misdiagnosed as DILI. The DDW-J 2004 scoring scale, current diagnostic scale for DILI, cannot discriminate acute hepatitis E cases from DILI cases because the scoring scale has no support for HEV infection. We propose that HEV-IgA test should be included in the current diagnostic scale for drug-induced liver injury for differential diagnosis of acute HEV infection to provide more accurate diagnosis of DILI in Japan. © 2014 The Japan Society of Hepatology. Source


Okano H.,Suzuka General Hospital | Takahashi M.,Jichi Medical University | Isono Y.,Suzuka General Hospital | Tanaka H.,Suzuka General Hospital | And 10 more authors.
Hepatology Research | Year: 2014

Aim: To characterize hepatitis E in Mie prefecture and to investigate whether raw pig liver sold as food in Mie is contaminated with hepatitis E virus (HEV) strains similar to those recovered from patients. Methods: Seventeen patients with sporadic acute hepatitis E treated from 2004 to 2012 were studied. A total of 243 packages of raw pig liver from regional grocery stores were tested for the presence of HEV RNA. The partial genomic sequences of human and swine HEV isolates were determined and subjected to the phylogenetic analyses. Results: The HEV isolates recovered from the 17 patients segregated into genotype 3 (n=15) and genotype 4 (n=2), and 15 genotype 3 isolates further segregated into 3e (n=11) and 3b (n=4). Pig liver specimens from 12 (4.9%) of the 243 packages had detectable HEV RNA. All 12 swine HEV isolates were grouped into genotype 3 (3a or 3b). Although no 3e strains were isolated from pig liver specimens, two 3b swine strains were 99.5-100% identical to two HEV strains recovered from hepatitis patients, within 412-nt partial sequences. Conclusion: The 3e HEV was prevalent among hepatitis E patients. HEV RNA was detected in approximately 5% of pig liver sold as food. The presence of identical HEV strains between hepatitis patients and pig liver indicated that pigs play an important role as reservoirs for HEV in humans in Mie. Further studies are needed to clarify the source of 3e HEV in the animal and environmental reservoirs. © 2013 The Japan Society of Hepatology. Source


Fujimoto S.,Kuwana West Medical Center | Ishida S.,Kuwana West Medical Center | Nakano T.,Fujita Health University Nanakuri Sanatorium | Kitagawa N.,Kuwana West Medical Center | And 9 more authors.
Acta Hepatologica Japonica | Year: 2014

We experienced a case of hepatitis E in Mie prefecture infected with genotype 4 hepatitis E virus (HEV) strain endemic in Aichi and Shizuoka prefectures (Aichi/Shizuoka strain). The HEV isolate obtained from the patient clustered with other Aichi/Shizuoka strains with 100% of bootstrap value in the phylogenetic tree, and was more than 97.8% identical to other Aichi/Shizuoka strains, in the 412-nucleotide sequence within the ORF2 region. Interestingly, the patient had no history of consuming wild animal meat although all other reported Aichi/Shizuoka strains were recovered from meat from wild animals or humans after eating wild animal meat. The finding indicated that the origin of Aichi/Shizuoka strains is confined not only to wild animal meat but also to other unknown reservoirs. © 2014 The Japan Society of Hepatology. Source


Inoue T.,Nagoya University | Sugiyama M.,Nagoya University | Hattori H.,Nagoya University | Wakita H.,Fujita Health University Nanakuri Sanatorium | And 2 more authors.
Tissue Engineering - Part A | Year: 2013

Regenerative therapy using stem cells is a promising approach for the treatment of stroke. Recently, we reported that dental pulp stem cells (DPSC) ameliorated ischemic tissue injury in the rat brain and accelerated functional recovery after middle cerebral artery occlusion (MCAO). In this study, we investigated the effects of stem cells from human exfoliated deciduous tooth (SHED)-derived conditioned medium (SHED-CM) on permanent MCAO (pMCAO). Adult male Sprague-Dawley rats were subjected to pMCAO. SHED-CM were then administered intranasally, and the motor function and infarct volume were evaluated. Neurogenesis and vasculogenesis were determined using immunochemical markers. The SHED-CM group had more positive signals than the Dulbecco's modified Eagle's medium group, with doublecortin (DCX), neurofilament H, neuronal nuclei, and rat endothelial cell antigen observed in the peri-infarct area. Migration of neuronal progenitor cells (NPC) with DCX from the subventricular zone to the peri-infarct area was observed on days 6 and 16, with migration on day 6 being the most prominent. In conclusion, SHED-CM promoted the migration and differentiation of endogenous NPC, induced vasculogenesis, and ameliorated ischemic brain injury after pMCAO as well as transplantation of DPSC. © Mary Ann Liebert, Inc. Source

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