Fujicco Co.

Kōbe-shi, Japan

Fujicco Co.

Kōbe-shi, Japan

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Yoshioka Y.,Kobe University | Li X.,Kobe University | Zhang T.,Kobe University | Mitani T.,Kobe University | And 5 more authors.
Journal of Clinical Biochemistry and Nutrition | Year: 2017

Black soybean seed coat, extract (BE), which contains abundant polyphenols such as procyanidins, cyanidin 3-glucoside, (+)-catechin, and (-)-epicatechin, has been reported on health beneficial functions such as antioxidant activity, anti-inflammatory, anti-obesity, and anti-diabetic activities. In this study, we investigated that prevention of BE and its polyphenols on 2,2'-azobis(2-methylpropionamide) dihydrochloride (AAPH)-induced oxidative DNA damage, and found that these polyphenols inhibited AAPH-induced formation of 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a biomarker for oxidative DNA damage in HepG2 cells. Under the same conditions, these polyphenols also inhibited AAPH-induced accumulation of reactive oxygen species (ROS) in the cells. Inhibition of ROS accumulation was observed in both cytosol and nucleus. It was confirmed that these polyphenols inhibited formation of AAPH radical using oxygen radical absorbance capacity assay under the cell-free conditions. These results indicate that polyphenols in BE inhibit free radical-induced oxidative DNA damages by their potent antioxidant activity. Thus, BE is an effective food material for prevention of oxidative stress and oxidative DNA damages. © 2017 JCBN.

Ito C.,Fujicco. Co. | Oki T.,Japan National Agricultural Research Center | Yoshida T.,Fujicco. Co. | Nanba F.,Fujicco. Co. | And 2 more authors.
Food Chemistry | Year: 2013

Proanthocyanidin oligomers (dimers to tetramers) were isolated from black soybean seed coats, using Sephadex LH-20 column chromatography and reversed-phase preparative HPLC. The isolated oligomers consisted of only (-)-epicatechin units, which were linked through either 4β→ 8 or 4β→ 6 (B-type) bonds. Procyanidin B2, procyanidin C1, and cinnamtannin A2 were identified as the main compounds of the proanthocyanidin dimers, trimers, and tetramers, respectively. © 2013 Elsevier Ltd. All rights reserved.

Kurimoto Y.,Chubu University | Shibayama Y.,Chubu University | Inoue S.,Chubu University | Soga M.,Chubu University | And 7 more authors.
Journal of Agricultural and Food Chemistry | Year: 2013

Black soybean seed coat has abundant levels of polyphenols such as anthocyanins (cyanidin 3-glucoside; C3G) and procyanidins (PCs). This study found that dietary black soybean seed coat extract (BE) ameliorates hyperglycemia and insulin sensitivity via the activation of AMP-activated protein kinase (AMPK) in type 2 diabetic mice. Dietary BE significantly reduced blood glucose levels and enhanced insulin sensitivity. AMPK was activated in the skeletal muscle and liver of diabetic mice fed BE. This activation was accompanied by the up-regulation of glucose transporter 4 in skeletal muscle and the down-regulation of gluconeogenesis in the liver. These changes resulted in improved hyperglycemia and insulin sensitivity in type 2 diabetic mice. In vitro studies using L6 myotubes showed that C3G and PCs significantly induced AMPK activation and enhanced glucose uptake into the cells. © 2013 American Chemical Society.

Yoshida T.,Fujicco Co.
Nippon Shokuhin Kagaku Kogaku Kaishi | Year: 2013

In soybean, a number of biologically active, and thus beneficial, substances have been identified; including isoflavone, lecithin, and saponin. Some of these are currently being marketed because their health promoting functions have been publicly approved. Moreover, recent evidence has accumulated suggesting that soybeans could contain other unused compounds with valuable functions as food additives. In this review, we focus on black soybean seed coat polyphenols (BSCP) and pinitol (PI). BSCP mainly consists of procyanidin, cyanidin 3-glucoside, and epicatechin, which are all well known for their antioxidant properties. Procyanidin represents a group of oligomeric compounds formed from catechin and epicatechin molecules. BSCP is characterized by its specific composition of procyanidin, and is particularly rich in smaller oligomer forms. It is thought that procyanidin oligomer size is inversely correlated to bioavailability. PI (3-O-methyl-D-chiro-inositol) is an inositol derivative that chiefly exists in legumes and pines, and is characterized by its extremely high water-solubility. PI content in soybean seed was reported to be around 0.2% dry weight, while in soybean plant, it is one of the major low-molecular weight carbohydrates; moreover, it is very rich in soybean leaves (up to 2% of dry weight). Intriguingly, both BSCP and PI exert anti-diabetic and anti-obesity effects. However, they have been under-utilized mainly due to a lack of efficient methods for stable and effective handling. Here we discuss both plausible mechanisms that could enable the exertion of beneficial functions and recent developments in their preparation from raw materials.

Kosaka A.,Japan National Institute of Advanced Industrial Science and Technology | Yan H.,Japan National Institute of Advanced Industrial Science and Technology | Ohashi S.,Fujicco Co. | Gotoh Y.,Fujicco Co. | And 5 more authors.
International Immunopharmacology | Year: 2012

Lactic acid bacteria (LAB) benefit health as probiotics in a strain-dependent way. In this study, we investigated the immunomodulatory effects of Lactococcus lactis subsp. cremoris FC (LcFC) on dendritic cells (DCs), natural killer (NK) cells and T cells. LcFC induced the production of cytokines such as IL-10, IL-12, IL-6 and TNF-α from murine bone marrow DCs (BMDCs) via MyD88-dependent pathway. In comparison with the type strain L. lactis subsp. cremoris ATCC 19257, LcFC induced particularly high production of IL-12 while induction of IL-6 was moderate. Consequently, LcFC triggered IFN-γ production in splenic NK, CD8+, and CD4+ cells. Most prominent effect of LcFC on IFN-γ production was observed in NK cells, followed by CD8+ cells, which was completely inhibited by combination of neutralizing anti-IL-12 and anti-IL-18 mAbs. Moreover, oral administration of LcFC enhanced the production of IFN-γ and IL-10 from splenocytes of treated mice. These findings suggest that this LAB strain is an efficient activator of protective cellular immunity via stimulation of myeloid cells including DCs. © 2012 Elsevier B.V. All rights reserved.

Jin J.S.,RIKEN | Touyama M.,RIKEN | Kibe R.,RIKEN | Tanaka Y.,RIKEN | And 12 more authors.
Beneficial Microbes | Year: 2013

The intestinal microbiota composition of 92 volunteers living in Japan was identified following the consumption of 'identical meals' (1,879 kcal/day) for 3 days. When faecal samples were analysed by terminal restriction fragment length polymorphism with several primer-restriction enzyme systems and then clustered, the patterns could be divided into 2 clusters. Contribution tests and partition modelling showed that OTU211 of the 35f-MspI system and OTU237 of the 35f-AluI system were key factors in the distribution of these groups. However, significant differences among these groups in terms of body mass index and age were not observed. ©2013 Wageningen Academic Publishers.

Kanamoto Y.,Kobe University | Yamashita Y.,Kobe University | Nanba F.,Fujicco Co. | Yoshida T.,Fujicco Co. | And 4 more authors.
Journal of Agricultural and Food Chemistry | Year: 2011

Black soybean seed coat extract (BE) is a polyphenol-rich food material consisting of 9.2% cyanidin 3-glucoside, 6.2% catechins, 39.8% procyanidins, and others. This study demonstrated that BE ameliorated obesity and glucose intolerance by up-regulating uncoupling proteins (UCPs) and down-regulating inflammatory cytokines in C57BL/6 mice fed a control or high-fat diet containing BE for 14 weeks. BE suppressed fat accumulation in mesenteric adipose tissue, reduced the plasma glucose level, and enhanced insulin sensitivity in the high-fat diet-fed mice. The gene and protein expression levels of UCP-1 in brown adipose tissue and UCP-2 in white adipose tissue were up-regulated by BE. Moreover, the gene expression levels of major inflammatory cytokines, tumor necrosis factor-α and monocyte chemoattractant protein-1 were remarkably decreased by BE in white adipose tissue. BE is a beneficial food material for the prevention of obesity and diabetes by enhancing energy expenditure and suppressing inflammation. © 2011 American Chemical Society.

Yamashita Y.,Kobe University | Wang L.,Kobe University | Nanba F.,Fujicco Co. | Ito C.,Fujicco Co. | And 2 more authors.
PLoS ONE | Year: 2016

Procyanidins are the oligomeric or polymeric forms of epicatechin and catechin. In this study, we isolated and purified dimer to tetramer procyanidins from black soybean seed coat and investigated the anti-hyperglycemic effects by focusing on glucose transporter 4 (GLUT4) translocation and the underlying molecular mechanism in skeletal muscle of mice. The anti-hyperglycemic effects of procyanidins were also compared with those of monomer (-)-epicatechin (EC) and major anthocyanin, cyanidin-3-O-β-glucoside (C3G). To investigate GLUT4 translocation and its related signaling pathways, ICR mice were orally given procyanidins, EC and C3G in water at 10 μg/kg body weight. The mice were sacrificed 60 min after the dose of polyphenols, and soleus muscle was extracted from the hind legs. The results showed that trimeric and tetrameric procyanidins activated both insulin-and AMPKsignaling pathways to induce GLUT4 translocation in muscle of ICR mice. We confirmed that procyanidins suppressed acute hyperglycemia with an oral glucose tolerance test in a dose-dependent manner. Of these beneficial effects, cinnamtannin A2, one of the tetramers, was the most effective. In conclusion, procyanidins, especially cinnamtannin A2, significantly ameliorate postprandial hyperglycemia at least in part by promoting GLUT4 translocation to the plasma membrane by activating both insulin-and AMPK-signaling pathways. © 2016 Yamashita et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PubMed | Miyagi University, Biofermin Kobe Research Institute, Toa Pharmaceutical Co., Yakult Central Institute for Microbiological Research and 4 more.
Type: Journal Article | Journal: Bioscience of microbiota, food and health | Year: 2014

The intestinal microbiota compositions of 92 men living in Japan were identified following consumption of identical meals for 3 days. Fecal samples were analyzed by terminal restriction fragment length polymorphism with 4 primer-restriction enzyme systems, and the 120 obtained operational taxonomic units (OTUs) were analyzed by Data mining software focusing on the following 5 characteristics, namely, age, body mass index, present smoking habit, cessation period of previous smokers and drinking habit, according to the answers of the subjects. After performing Data mining analyses with each characteristic, the details of the constructed Decision trees precisely identified the subjects or discriminated them into various suitable groups. Through the pathways to reach the groups, practical roles of the related OTUs and their quantities were clearly recognized. Compared with the other identification methods for OTUs such as bicluster analyses, correlation coefficients and principal component analyses, the clear difference of this Data mining technique was that it set aside most OTUs and emphasized only some closely related ones. For example for a selected characteristic, such as smoking habit, only 7 OTUs out of 120 were able to identify all smokers, and the remaining 113 OTUs were thought of as data noise for smoking. Data mining analyses were affirmed as an effective method of subject discrimination for various physiological constitutions. The species of bacteria that were closely related to heavy smokers, i.e., HaeIII-291, were also discussed.

PubMed | Fujicco Co.
Type: Journal Article | Journal: Letters in applied microbiology | Year: 2012

To evaluate the protective effects of oral administration of milk fermented with a Lactococcus strain against influenza virus (IFV) infection in a mouse model.Milk fermented with exopolysaccharide-producing Lactococcus lactis subsp. cremoris (L. cremoris) FC was orally administered to BALB/c mice for 12 days. Mice were intranasally infected with IFV A/New Caledonia/20/99 (H1N1) on day 8, and survival was determined for 14 days after IFV infection. Survival rate and body weight loss after IFV infection in the L. cremoris FC fermented milk-administered group were significantly improved compared with those in the control group. In the unfermented milk-administered group, survival rate was not improved, whereas body weight loss was slightly improved compared with that in the control group. The mean virus titre in the lung of the L. cremoris FC fermented milk-administered group 3 days after infection was significantly decreased compared with that in the control group.These results suggest that oral administration of milk fermented with L. cremoris FC protects mice against IFV infection.These results demonstrate that oral administration of milk fermented with exopolysaccharide-producing Lactococcus strains might protect host animals against IFV infection.

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