Time filter

Source Type

Zhang H.,Chinese Academy of Agricultural Sciences | Wu S.,Chinese Academy of Agricultural Sciences | Xing Z.,Chinese Academy of Agricultural Sciences | Wang X.,Plant Protection Station of Beijing | And 2 more authors.
Journal of Economic Entomology | Year: 2016

When flies were dipped in 1×108 conidia/ml conidia suspensions and then kept in the incubator (22±1 °C, 7065% RH), scanning electron microscope observations revealed that, at 2 h, the majority of adhering Beauveria bassiana conidia were attached to either the wing surface or the interstitial area between the macrochaetae on the thorax and abdomen of the onion maggot adults. Germ tubes were being produced and had oriented toward the cuticle by 18 h. Penetration of the insect cuticle had occurred by 36 h, and by 48 h, germ tubes had completely penetrated the cuticle. Fungal mycelia had emerged from the insect body and were proliferating after 72 h. The superficial area and structure of the wings and macrochaetae may facilitate the attachment of conidia and enable effective penetration. The susceptibility of adults to 12 isolates, at a concentration of 1×107 conidia/ml, was tested in laboratory experiments. Eight of the more potent strains caused in excess of 85% adult mortality 8 d post inoculation, while the median lethal time (LT50) of these strains was <6 d. The virulence of the more effective strains was further tested, and the median lethal concentrations (LC50) were calculated by exposing adults to doses ranging from 103-107 conidia/ml. The lowest LC50 value, found in the isolate XJWLMQ-32, for the adults was 3.87×103 conidia/ml. These results demonstrate that some B. bassiana strains are highly virulent to onion maggot adults and should be considered as potential biocontrol agents against the adult flies. © The Authors 2016.


He X.,Hubei University | He X.,Fujian Taiwan Joint Center for Ecological Control of Crop Pests | He X.,Key Laboratory of Biopesticide and Chemical Biology | Du X.,Hubei University | And 16 more authors.
Natural Product Research | Year: 2015

A furanone (1), (S)-methyl 2-(2-hydroxy-3,4-dimethyl-5-oxo-2,5-dihydrofuran-2-yl)acetate, was isolated from the edible mushroom Grifola frondosa. Mass spectrometry and NMR analyses were used to elucidate the structure of this compound, and its absolute configuration was determined using circular dichroism spectroscopy. Compound 1 exhibited specific antifungal activity against the plant pathogens, Fusarium oxysporum, Gibberella zeae and Piricularia oryzae and the opportunistic human pathogen, Pseudallescheria boydii, resulting in minimum inhibitory concentration values of 2.5, 2.5, 1.25 and 0.15 μg/mL, respectively. In contrast, the furanone showed only weak activity towards Aspergillus spp., Candida albicans and several other fungal strains tested as well as no appreciable antibacterial activity. © 2015 Taylor & Francis


Huang T.,Fujian Taiwan Joint Center for Ecological Control of Crop Pests | Pan J.,Centers for Disease Control and Prevention | Guan X.,Fujian Taiwan Joint Center for Ecological Control of Crop Pests
Scientific Reports | Year: 2016

Bacillus thuringiensis (Bt), one of the most successful biopesticides, may expand its potential by producing bacteriocins (thuricins). The aim of this study was to investigate the antimicrobial potential of a novel Bt bacteriocin, thuricin BtCspB, produced by Bt BRC-ZYR2. The results showed that this bacteriocin has a high similarity with cold-shock protein B (CspB). BtCspB lost its activity after proteinase K treatment; however it was active at 60 °C for 30 min and was stable in the pH range 5-7. The partial loss of activity after the treatments of lipase II and catalase were likely due to the change in BtCspB structure and the partial degradation of BtCspB, respectively. The loss of activity at high temperatures and the activity variation at different pHs were not due to degradation or large conformational change. BtCspB did not inhibit four probiotics. It was only active against B. cereus strains 0938 and ATCC 10987 with MIC values of 3.125 μg/mL and 0.781 μg/mL, and MBC values of 12.5 μg/mL and 6.25 μg/mL, respectively. Taken together, these results provide new insights into a novel cold shock protein-like bacteriocin, BtCspB, which displayed promise for its use in food preservation and treatment of B. cereus-associated diseases. © 2016 The Author(s).


Wei Y.,Fujian Academy of Agricultural science | Wei Y.,Fujian Taiwan Joint Center for Ecological Control of Crop Pests | Wei Y.,Incubator of National Key Laboratory of Crop Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of science and Technology Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China | Xu H.,Fujian Academy of Agricultural science | And 23 more authors.
Plant Molecular Biology | Year: 2015

Damaged proteins containing abnormal isoaspartyl (isoAsp) accumulate as seeds age and the abnormality is thought to undermine seed vigor. Protein-l-isoaspartyl methyltransferase (PIMT) is involved in isoAsp-containing protein repair. Two PIMT genes from rice (Oryza sativa L.), designated as OsPIMT1 and OsPIMT2, were isolated and investigated for their roles. The results indicated that OsPIMT2 was mainly present in green tissues, but OsPIMT1 largely accumulated in embryos. Confocal visualization of the transient expression of OsPIMTs showed that OsPIMT2 was localized in the chloroplast and nucleus, whereas OsPIMT1 was predominately found in the cytosol. Artificial aging results highlighted the sensitivity of the seeds of OsPIMT1 mutant line when subjected to accelerated aging. Overexpression of OsPIMT1 in transgenic seeds reduced the accumulation of isoAsp-containing protein in embryos, and increased embryo viability. The germination percentage of transgenic seeds overexpressing OsPIMT1 increased 9–15 % compared to the WT seeds after 21-day of artificial aging, whereas seeds from the OsPIMT1 RNAi lines overaccumulated isoAsp in embryos and experienced rapid loss of seed germinability. Taken together, these data strongly indicated that OsPIMT1-related seed longevity improvement is probably due to the repair of detrimental isoAsp-containing proteins that over accumulate in embryos when subjected to accelerated aging. © 2015, Springer Science+Business Media Dordrecht.


PubMed | Fujian Taiwan Joint Center for Ecological Control of Crop Pests and Fujian Academy of Agricultural science
Type: Journal Article | Journal: Plant molecular biology | Year: 2015

Damaged proteins containing abnormal isoaspartyl (isoAsp) accumulate as seeds age and the abnormality is thought to undermine seed vigor. Protein-L-isoaspartyl methyltransferase (PIMT) is involved in isoAsp-containing protein repair. Two PIMT genes from rice (Oryza sativa L.), designated as OsPIMT1 and OsPIMT2, were isolated and investigated for their roles. The results indicated that OsPIMT2 was mainly present in green tissues, but OsPIMT1 largely accumulated in embryos. Confocal visualization of the transient expression of OsPIMTs showed that OsPIMT2 was localized in the chloroplast and nucleus, whereas OsPIMT1 was predominately found in the cytosol. Artificial aging results highlighted the sensitivity of the seeds of OsPIMT1 mutant line when subjected to accelerated aging. Overexpression of OsPIMT1 in transgenic seeds reduced the accumulation of isoAsp-containing protein in embryos, and increased embryo viability. The germination percentage of transgenic seeds overexpressing OsPIMT1 increased 9-15% compared to the WT seeds after 21-day of artificial aging, whereas seeds from the OsPIMT1 RNAi lines overaccumulated isoAsp in embryos and experienced rapid loss of seed germinability. Taken together, these data strongly indicated that OsPIMT1-related seed longevity improvement is probably due to the repair of detrimental isoAsp-containing proteins that over accumulate in embryos when subjected to accelerated aging.


Wei X.,Fujian Agriculture and forestry University | Wei X.,Fujian Taiwan Joint Center for Ecological Control of Crop Pests | Wei X.,CAS Institute of Microbiology | Song X.,Fujian Agriculture and forestry University | And 22 more authors.
Canadian Journal of Microbiology | Year: 2016

The insect pathogenic fungus Aschersonia placenta is a highly effective pathogen of whiteflies and scale insects. However, few genetic tools are currently available for studying this organism. Here we report on the conditions for the production of transformable A. placenta protoplasts using an optimized protocol based on the response surface method (RSM). Critical parameters for protoplast production were modelled by using a Box- Behnken design (BBD) involving 3 levels of 3 variables that was subsequently tested to verify its ability to predict protoplast production (R2 = 0.9465). The optimized conditions resulted in the highest yield of protoplasts ((4.41 ± 0.02) × 107 cells/mL of culture, mean ± SE) when fungal cells were treated with 26.1 mg/mL of lywallzyme for 4 h of digestion, and subsequently allowed to recover for 64.6 h in 0.7 mol/L NaCl-Tris buffer. The latter was used as an osmotic stabilizer. The yield of protoplasts was approximately 10-fold higher than that of the nonoptimized conditions. Generated protoplasts were transformed with vector PbarGPE containing the bar gene as the selection marker. Transformation efficiency was 300 colonies/(µg DNA.107 protoplasts), and integration of the vector DNA was confirmed by PCR. The results show that rational design strategies (RSM and BBD methods) are useful to increase the production of fungal protoplasts for a variety of downstream applications. © 2016 Published by NRC Research Press.


Huang T.,Fujian Agriculture and forestry University | Huang T.,Fujian Taiwan Joint Center for Ecological Control of Crop Pests | Yu X.,Fujian Agriculture and forestry University | Gelbic I.,Academy of Sciences of the Czech Republic | And 2 more authors.
Canadian Journal of Microbiology | Year: 2015

Gene expression profiles are important data to reveal the functions of genes putatively involved in crucial biological processes. RNA arbitrarily primed polymerase chain reaction (RAP-PCR) and specifically primed reverse transcription polymerase chain reaction (RT-PCR) were combined to screen differentially expressed genes following development of a commercial Bacillus thuringiensis subsp. kurstaki strain 8010 (serotype 3a3b). Six differentially expressed transcripts (RAP1 to RAP6) were obtained. RAP1 encoded a putative triple helix repeat-containing collagen or an exosporium protein H related to spore pathogenicity. RAP2 was homologous to a ClpX protease and an ATP-dependent protease La (LonB), which likely acted as virulence factors. RAP3 was homologous to a beta subunit of propionyl-CoA carboxylase required for the development of Myxococcus xanthus. RAP4 had homology to a quinone oxidoreductase involved in electron transport and ATP formation. RAP5 showed significant homology to a uridine kinase that mediates phosphorylation of uridine and azauridine. RAP6 shared high sequence identity with 3-methyl-2-oxobutanoate-hydroxymethyltransferase (also known as ketopantoate hydroxymethyltransferase or PanB) involved in the operation of the tricarboxylic acid cycle. The findings described here would help to elucidate the molecular mechanisms underlying the differentiation process of B. thuringiensis and unravel novel pathogenic genes. © 2015, National Research Council of Canada. All rights reserved.


Tianpei H.,Fujian Agriculture and forestry University | Tianpei H.,Fujian Taiwan Joint Center for Ecological Control of Crop Pests | Jie J.,Fujian Agriculture and forestry University | Xiong G.,Fujian Agriculture and forestry University | Xiong G.,Fujian Taiwan Joint Center for Ecological Control of Crop Pests
Research Journal of Biotechnology | Year: 2016

Seventy-seven Bacillus thuringiensis (Bt) strains, isolated from diverse resources had been investigated for their regulation on plant growth. Chinese cabbage was selected as tested plant. Height, fresh weight and number of leaves of the seedlings were measured after 30 days cultivation. Of the 77 Bt strains, 32 strains promoted the growth while 28 strains inhibited Chinese cabbage production, indicating that Bt regulates Chinese cabbage growth beyond growth promotion Interestingly, Bt plant-growth promoting strains BRC-CWS5, BRC-SFR2, BRC-SFR6 and HQ20 produced indole-3-acetic acid (IAA) in LB liquid media supplementing with tryptophan but they were not able to soluble phosphate. The results suggested that IAA production and phosphate-solubilizing ability may not be universal mechanisms for plant-growth-promoting Bt strains. Our data implied that both good effects (insecticidal spectrum, plant growth promotion etc.) and potential adverse effects (plant growth inhibition etc.) should be considered when choosing a new Bt strain for production. © 2016, Research Journal of BioTechnology. All rights reserved.

Loading Fujian Taiwan Joint Center for Ecological Control of Crop Pests collaborators
Loading Fujian Taiwan Joint Center for Ecological Control of Crop Pests collaborators