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Shangguan J.B.,Jimei University | Shangguan J.B.,Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco environment | Li Z.B.,Jimei University | Li Z.B.,Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco environment | And 6 more authors.
Genetics and Molecular Research | Year: 2014

Fenneropenaeus penicillatus, with high protein and low fat, is a commercially important aquatic product in China. Microsatellite loci were developed according to the protocol of fast isolation by amplified fragment length polymorphism of sequences containing repeats. Eight new polymorphic microsatellite markers for F. penicillatus were identified, and 32 wild individuals were used to evaluate the degree of polymorphism of these markers. The polymorphism information content ranged from 0.2703 to 0.7598, and the number of alleles per locus varied from 3 to 6. The observed and expected heterozygosities were 0.1613-0.5556 and 0.2347-0.7387, respectively. No signifi cant deviations from Hardy-Weinberg equilibrium (P > 0.00625) were detected. These polymorphic microsatellite loci will be useful to study the genetic diversity and population structure of F. penicillatus. © FUNPEC-RP. Source


Li Z.B.,Jimei University | Li Z.B.,Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco environment | Li Q.H.,Jimei University | Li Q.H.,Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco environment | And 6 more authors.
Genetics and Molecular Research | Year: 2015

Lunella coronata granulata, from the family Turbinidae, is an economically important species. The first set of 10 polymorphic microsatellite loci was screened from L. coronata granulata, and 30 individuals were used to analyze the degree of polymorphism in these loci. The level of observed and expected heterozygosity was 0.0667-0.7333 and 0.0644-0.6628, respectively. The polymorphism information content varied from 0.305 to 0.559. Eight loci were in Hardy-Weinberg equilibrium (HWE) (P > 0.05), while two loci deviated significantly from the HWE after Bonferroni’s correction (P < 0.005). The isolated microsatellite loci can be utilized in studies of population genetic analysis and they provide important genetic markers for construction of genetic linkage maps and genetic breeding of L. coronata granulata resources. © FUNPEC-RP. Source


Li Z.B.,Jimei University | Li Z.B.,Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco environment | Huang Y.S.,Jimei University | Huang Y.S.,Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco environment | And 8 more authors.
Genetics and Molecular Research | Year: 2015

Branchiostoma belcheri Gray is a second-class, nationally protected protochordate in China. We developed 10 novel polymorphic sites in B. belcheri, which were examined using a population of 30 wild individuals from Xiamen, China. The polymorphism information content ranged between 0.141 and 0.681, and the number of alleles varied from 2 to 5. The expected and observed heterozygosities varied between 0.1528 and 0.6920, and between 0.1429 and 0.5000, respectively. These novel microsatellite markers will facilitate the genetic analysis and protection of wild B. belcheri individuals, and the possible re-stocking of the species in the long-term. © FUNPEC-RP. Source


Huang Y.S.,Jimei University | Huang Y.S.,Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco environment | Li Q.H.,Jimei University | Li Q.H.,Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco environment | And 8 more authors.
Genetics and Molecular Research | Year: 2015

The pen shell, Atrina vexillum Born, is an edible shellfish that is widely consumed in the Asia-Pacific region. In this study, 11 polymorphic microsatellite loci were isolated from A. vexillum, and 30 wild individuals were used to evaluate the degree of polymorphism of these markers. The number of alleles per locus ranged from 3 to 8. The polymorphism information content varied from 0.199 to 0.831. The observed and expected heterozygosities were 0.1000-0.8667 and 0.1244-0.8356, respectively. Two loci deviated significantly from the Hardy-Weinberg equilibrium (HWE) after a Bonferroni correction, while the other nine loci were at HWE. These microsatellite loci will be useful in further studies on population genetic analyses, and will provide important genetic data for the conservation and recovery of A. vexillum. © FUNPEC-RP. Source


Li Z.B.,Jimei University | Li Z.B.,Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco environment | Tian X.J.,Jimei University | Tian X.J.,Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco environment | And 8 more authors.
Genetics and Molecular Research | Year: 2015

Twelve microsatellite loci were developed from Haliotis ovina by the magnetic bead hybridization method. Genetic variability was assessed using 30 individuals from 3 wild populations. The number of alleles per locus ranged from 2 to 5, and the polymorphism information content ranged from 0.1228 to 0.6542. Observed and expected heterozygosities ranged from 0.0000 to 0.7778 and 0.1288 to 0.6310, respectively. These loci should provide useful information for genetic studies such as genetic diversity, pedigree analysis, construction of genetic linkage maps, and marker-assisted selection breeding in H. ovina. ©FUNPEC-RP. Source

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