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Zheng Z.,Fujian Provincial Geriatric Hospital
Chinese Journal of Cerebrovascular Diseases | Year: 2016

Objective: To observe the effect of pyridoxamine on advanced glycosylation end-products ( AGEs) and their receptors in arteriosclerotic rabbits. Methods: A rabbit atherosclerotic model was induced and established by using a high fat diet. The New Zealand white rabbits were divided into a normal control group, an atherosclerosis group, a low-dose pyridoxamine group, and a high-dose pyridoxamine group according to the random number table method. The normal control group was given normal diet; the atherosclerosis group was given high-fat diet; the low-dose pyridoxamine group was given high-fat diet and pyridoxamine 100 mg/( kg · d) ;and the high-dose pyridoxamine group was given high-fat diet and pyridoxamine 150 mg/ (kg · d). All rabbits were observed for 12 weeks. Twelve weeks after the experiment, the blood glucose, triglyceride, total cholesterol, low-density lipoprotein cholesterol ( LDL-C) , and high-density lipoprotein cholesterol ( HDL-C ) in all rabbits were monitored. The enzyme-linked immunosorbent assay was used to detect the serum advanced AGEs level. The changes of aortic pathological morphology were observed. Immunohistochemistry was used to detect the aortic receptor of AGEs (RAGE) content, and the comparison among the groups was conducted. Results: Twelve weeks after the experiment: ( 1 ) compared with the normal control group, the triglyceride, total cholesterol, LDL-C, HDL-C were increased significantly ( P < 0.01) in the atherosclerosis group, but there was no significant change in glucose (P > 0. 05). Compared with the control group, the serum AGEs level (419 ±29 mg/L) , artery RAGE absorbance (0.88±0. 14),and plaque area (67.4±5.8%) were increased significantly (141 ±11 mg/L,0. 19 ± 0. 04 and 0% , respectively ; all P < 0. 01). (2 ) Compared with the atherosclerosis group, the triglyceride, total cholesterol, LDL-C, HDL-C, and glucose did not have significantly change in the low-dose pyridoxamine group and the high-dose pyridoxamine group (all P > 0.05). Compared with the atherosclerosis group,the serum level of AGEs (278 ± 22 mg/L) , artery RAGE absorbance (0. 43 ± 0. 06), and plaque area (43. 2 ± 2.4% ) in the low-dose pyridoxamine group,and the serum level of AGEs (211 ±18 mg/L) ,artery RAGE absorbance (0.27 ±0.05), and plaque area (27.4 ±2. 8%) were all decreased significantly in the high-dose pyridoxamine group (all P < 0.01). (3) The serum level of AGEs, artery RAGE absorbance, and plaque area in the high-dose pyridoxamine group were lower than those in the low-dose pyridoxamine group (P < 0.01). (4) Aortic pathological morphology: the arterial intimal thickening and formation of obvious atherosclerotic plaque were observed under microscope in the atherosclerosis group. There were a large number of foam cell aggregation and more inflammatory cells. Compared with the atherosclerosis group, the low-dose pyridoxamine group and the high-dose pyridoxamine group had varying degrees of relief. Conclusion: Pyridoxamine may inhibit and slow down the formation of atherosclerosis in rabbits with experimental high-fat diet, and inhibit the AGEs generation and expression of RAGE in artery.


Cai W.-Q.,Fujian Medical University | Wang J.-S.,Fujian Medical University | Su J.-Z.,Fujian Medical University | Jiang J.-F.,Fujian Medical University | Yao Y.-X.,Fujian Provincial Geriatric Hospital
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: Previous studies have demonstrated that hepatocyte growth factor (HGF) gene transfection can improve the effectiveness of bone marrow mesenchymal stem cell transplantation, but the mechanism is still unclear. OBJECTIVE: To observe the effects of HGF gene transfection on c-MET, Bax, Bcl-2, Caspase-3 of bone marrow mesenchymal stem cells cultured under hypoxia and serum-free conditions. METHODS: (1) Bone marrow mesenchymal stem cells were isolated and amplified in vitro by differential adhesion method. The infection efficiency of recombinant adenovirus Ad-HGF in bone marrow mesechymal stem cells was tested by x-gal staining. (2) Bone marrow mesenchymal stem cells were cultured under hypoxia and serum-free conditions for 0, 3, 6, 9, 12 hours. RT-PCR and western blot assays were used to evaluate the expression of Bax, Bcl-2, Caspase-3. (3) Bone marrow mesenchymal stem cells were cultured under hypoxia and serum-free conditions for 6 hours, and RT-PCR and western blot assays were adopted to detect HGF, c-Met, Bax, Bcl-2 and Caspase-3. (4) Cell scratch test was used to detect the effect of HGF transfection on the migration of bone marrow mesenchymal stem cells cultured under hypoxia and serum-free conditions for 6 hours. RESULTS AND CONCLUSION: (1) Transfection efficiency of bone marrow mesenchymal stem cells was increased with multiplicity of infection in a dose-dependent manner. When the multiplicity of infection was 150, the transfection efficiency was 96.4%. (2) Expressions of Bax and Bcl-2 were gradually increased with hypoxia time (P < 0.05). The Bax/Bcl-2 ratio and Caspase-3 expression reach the minimum at 6 hours of hypoxia (P < 0.05). (3) Compared with the control and Ad-LacZ groups, the expressions of HGF, c-Met, Bcl-2 increased, and the expressions of Bax and Caspase-3 decreased in the Ad-HGF group after 6 hours of culture under hypoxia and serum-free conditions (P < 0.05). There was no significant difference between the control and Ad-LacZ groups. (4) The mobility of bone marrow mesenchymal stem cells was higher in the Ad-HGF group than the control group and Ad-LacZ groups after 6 hours of culture under hypoxia (P < 0.05). These findings indicate that transfection of HGF in bone marrow mesenchymal stem cells can increase the expression of c-Met, Bcl-2 and decrease the expression of Bax, Caspase-3 under hypoxia and serum-free conditions, which also enhance the mobility of bone marrow mesenchymal stem cells under hypoxia and serum-free conditions. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.


Chen G.,Fujian Medical University | Li M.,Fujian Medical University | Xu Y.,Fujian Medical University | Chen N.,Fujian Medical University | And 8 more authors.
Diabetes Technology and Therapeutics | Year: 2012

Objective: This study investigated the impact of family history of diabetes (FHD) on β-cell function among Chinese with normal glucose tolerance. Research Design and Methods: A multistage, stratified, cluster random sampling method was used to select a provincially representative sample from Fujian Province. Eventually, a total of 1,183 subjects were entered into the analysis. Several indexes were used to assess the function of β cells, including homeostasis model assessment (HOMA) of insulin resistance (IR), HOMA of β cells, insulinogenic index (IGI), and disposition index. Results: Overweight, increased body mass index, higher low-density lipoprotein cholesterol, and higher total cholesterol (TC) were the dominant features of positive FHD (FHD +). The FHD + subjects had lower insulin sensitivity (P<0.05). FHD + subjects showed higher risk of IR after adjusting for other risk factors (odds ratio 1.523 [1.272-2.009]). However, there was no significant difference in insulin secretion between the two groups. With the use of the multiple linear regression model, waist circumference (WC) and triglycerides (TGs) were found to be independent risk factors of the decline of insulin sensitivity in FHD + subjects, and insulin sensitivity declined significantly (P<0.05) with the increase of WC and TGs. In addition, the offspring of fathers with diabetes (PT2D) were much older and had higher TC than those of mothers with diabetes (MT2D). After adjusting for gender of the parents, there was no difference between MT2D and PT2D on insulin sensitivity. Conclusions: Inheritance if diabetes is associated with the decline of insulin sensitivity. In addition, insulin sensitivity declined with increasing WC and TG in FHD + subjects. © Copyright 2012, Mary Ann Liebert, Inc. 2012.

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