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Liang D.,Central South University | Lv W.,Central South University | Wang H.,Women and Childrens Hospital of Hunan Province | Xu L.,Fujian Provincial Maternity and Children Health Hospital | And 5 more authors.
Prenatal Diagnosis | Year: 2013

Objective: To determine whether non-invasive prenatal testing by maternal plasma DNA sequencing can uncover all fetal chromosome aneuploidies in one simple sequencing event. Methods: Plasma samples from 435 pregnant women at high risk for Down syndrome were collected prior to amniocentesis in three hospitals in China between March 2009 and June 2011. We sequenced the plasma DNA extracted from these samples at low coverage. We discovered that the genome representation of each of the 24 chromosomes obeyed a linear relationship to its GC content. Applying this relationship, we analysed the copy number of each of the 24 chromosomes. Full fetal karyotyping was compared with maternal plasma DNA sequencing results. Results: Among the 435 samples, 412 samples (94.7%) have full karyotyping and sequencing results. Sixty-seven samples containing a fetal chromosome aneuploidy, including trisomy 21, trisomy 18, trisomy 13, trisomy 9, monosomy X or others, can be accurately identified with a detection sensitivity of 100% and a detection specificity of 99.71%. Normalization of the chromosome representation values against chromosomal guanine/cytosine base content is the key issue to ensure the accuracy. Conclusions: Our results indicate that non-invasive detection of fetal chromosome aneuploidies for all 24 chromosomes in one single sequencing event is feasible. © 2013 John Wiley & Sons, Ltd.

Lemyre B.,The Ottawa Hospital | Xiu W.,Fujian Provincial Maternity and Children Health Hospital | Bouali N.R.,The Ottawa Hospital | Brintnell J.,The Ottawa Hospital | And 3 more authors.
Infection Control and Hospital Epidemiology | Year: 2012

Objective: Most cases of necrotizing enterocolitis (NEC) are sporadic, but outbreaks in hospital settings suggest an infectious cause. Our neonatal intensive care unit (NICU) experienced an outbreak of methicillin-sensitive Staphylococcus aureus (MSSA). We aimed to assess whether the enhancement of infection prevention and control measures would be associated with a reduction in the number of cases of NEC. Design: Retrospective chart review. Setting: A 24-bed, university-affiliated, inborn level 3 NICU. participants. Infants of less than 30 weeks gestation or birth weight ≤ 1,500 g admitted to the NICU between January 2007 and December 2008 were considered at risk of NEC. All cases of NEC were reviewed. interventions. Infection prevention and control measures, including hand hygiene education, were enhanced during the outbreak. Avoidance of overcapacity in the NICU was reinforced, environmental services (ES) measures were enhanced, and ES hours were increased. Results: Two hundred eighty-two at-risk infants were admitted during the study. Their gestational age and birth weight (mean ± SD) were weeks and g, respectively. The proportion of NEC was 18/110 (16.4%) before the outbreak, 1/54 (1.8%) 28.2±2.7 1,031±290 during the outbreak, and 4/118 (3.4%) after the outbreak. After adjustment for gestational age, birth weight, gender, and singleton versus multiple births, the proportion was lower in the postoutbreak period than in the preoutbreak period (P <.002). conclusion. Although this observational study cannot establish a causal relationship, there was a significant decrease in the incidence of NEC following implementation of enhanced infection prevention and control measures to manage an MSSA outbreak. © 2011 by The Society for Healthcare Epidemiology of America. All rights reserved.

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