Jieju L.,Fujian Medical University |
Jieju L.,Fujian Key Laboratory of Translational Medicine |
Mingshui C.,Fujian Medical University |
Mingshui C.,Fujian Key Laboratory of Translational Medicine |
And 7 more authors.
Chinese Journal of Cancer Biotherapy | Year: 2015
Objective: To evaluate the cytotoxic effects of the cytotoxic T lymphocytes (CTLs) induced by dendritic cells (DCs) loaded with alpha-fetoprotein (AFP) in different approaches. Methods: Peripheral blood monocytes were isolated from healthy donors. The adhesive precursor DC s were cultured in the presence of rhGM-CSF and rhIL—4 for 6 d. The cytokine-treated DCs were then left untreated as a control , infected with recombinant AFP-carrying adeno-associated viral vector (rAAV/AFP) , or loaded respectively with AFP peptide , HepG2 cell lysate , and HepG2-derived exosomes (T-exo). Changes in CD83 , CD86 , ICAM-1,CD58 And CD 40 in DCs before and after AFP loading were assessed by Western botting Autologous T cells were co-cultured with the various AFP-loaded DCs loaded at a ratio of 10: 1 for 48 h. The proliferative activity and cytotoxicity against HepG2 cells of DC-induced T cells were assessed by flow cytometry. Results: AFP antigen induced maturation of DCs. The expression of surface molecules CD83 , CD86, ICAM-1 , CD58 and CD40 in DCs infected with the rAAV/AFP vector was significantly increased compared with the naïve DCs (P <0. 05). AFP-loaded DCs increased the proliferation of T cells. The cytotoxic activity against HepG2 cells was (41.40 ±2. 87)% , (44. 9 ±4. 12)% , (28. 42 ± 3. 29)% , and (24. 28 ± 1. 79)% for T cells after induction by T-exo-loaded DCs, rAAV/ AFP-infected DCs, AFP peptide-loaded DCs and HepG2 lysate-treated DCs respectively (P <0. 05). Conclusions:A FP and T-exso e are capable of increasing the proliferative activity of s and the cytotoxic activity of TL against HepG2 cells. Our finding may have significant implications in the development of -based vaccines for liver cancer. © 2015, Editorial office of Chinese Journal of Cancer Biotherapy. All rights reserved.