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Wang Y.-Y.,Fuzhou University | Li Y.-N.,Fujian Key Laboratory of Medical Devices and Pharmaceutical Technology | Guo Y.-H.,Fuzhou University | Guo Y.-H.,Fujian Key Laboratory of Medical Devices and Pharmaceutical Technology
Progress in Biochemistry and Biophysics | Year: 2010

To prepare Citrinin (CIT)-protein antigen, CIT was conjugated with bovine serum albumin (BSA) by 1,4-butanediol diglycidyl ether. HPLC, UV and IR absorption suggested that CIT was correlated with the carrier protein, and the molar ratio of CIT to BSA was 8.16. The polyclonal antibodies (PcAb) against CIT were produced in serum of immunized BALB/C mice with CIT-BSA, and the titer of antibody reached to 1.1 × 105 by indirect enzyme-linked immunoassay. The indirect competitive ELISA showed that the detection limit of CIT was 10 μ.g/L, with a good linearity ranging 10∼250 μg/L, and IC50 was 100 μg/L. Immunogenicity of antigens prepared by different methods was analyzed. The result showed that the epitope was the carboxyl group at C7. This work would be helpful for establishing the technology and developing the kit to determine CIT-contaminated samples by ELISA. Source


Nie D.,Fuzhou University | Wu H.,Fuzhou University | Zheng Q.,Fuzhou University | Guo L.,Fuzhou University | And 5 more authors.
Chemical Communications | Year: 2012

A sensitive and selective Pb 2+ sensor based on GR-5 DNAzyme has been developed by a flow cytometric method. © The Royal Society of Chemistry 2012. Source


Li Y.,Fuzhou University | Li Y.,Fujian Health College | Wu H.,Fuzhou University | Guo L.,Fuzhou University | And 3 more authors.
Food Chemistry | Year: 2012

The determination of citrinin (CIT) by a microsphere-based flow cytometric immunoassay (MFCI) has been developed. In the method, the carboxyl-modified microspheres were conjugated with CIT-Ovalbumin (OVA) antigen. CIT competed with the CIT-OVA antigen on the surface of the microspheres for the anti-CIT McAb. Under the optimised conditions, IC50 value was 1.0 ng/mL and the limit of detection reached 0.005 ng/mL. The cross-reactivity was less than 0.01% against each of the four mycotoxins such as aflatoxin B1 (AFB 1), ochratoxin A (OTA), zearalenone (ZEA), deoxynilvalenol (DON). In the work, the MFCI could accurately determine CIT in the real red yeast rice. The systematic error was low with the coefficient of variation (CV) from 5.24% to 8.16% by the MFCI. The mean recovery of CIT from artificially contaminated red yeast rice was from 89% to 94%, with CV from 7.2% to 8.7%. The experimental data showed that the precision, sensitivity and specificity of the developed MFCI method for the determination of CIT were satisfactory. © 2012 Elsevier Ltd. All rights reserved. Source


Li Y.-N.,Fuzhou University | Wang Y.-Y.,Fujian Key Laboratory of Medical Devices and Pharmaceutical Technology | Zheng Y.-Q.,Fujian Key Laboratory of Medical Devices and Pharmaceutical Technology | Guo Y.-H.,Fuzhou University | Guo Y.-H.,Fujian Key Laboratory of Medical Devices and Pharmaceutical Technology
Progress in Biochemistry and Biophysics | Year: 2010

To prepare citrinin(CIT)-protein antigen, CIT was conjugated with bovine serum albumin (BSA) by 1, 4-butanediol diglycidyl ether. The spleen from the BALB/C mice immunized with CIT-BSA conjugate was used to fuse with the murine SP2/0. By subcloning, a hybridoma cell lines excreting monoclonal antibodies(McAb) against CIT was obtained and named H2-F8. The monoclonal antibodies obtained from hybridoma H2-F8 was of IgM subclass. The affinity constant of the McAb to CIT was 4.17×10 8 L/mol and the IC 50 value was 0.3 μg/L. Its linear range of the assay was between 0.05 μg/L and 1.0 μg/L. The cross-reactivity rates were less than 0.1% of other toxins such as ochratoxin A, aflatoxin B1, deoxynivalenol, zearalenone and were less than 0.01% of rubropunctatine and rubropunctamine. This work would be helpful for establishing the technology and developing the kit to determine CIT-contaminated samples by ELISA. Source

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