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Zhang L.,Fujian Agriculture and forestry University | Tang B.,Fujian Agriculture and forestry University | Huang E.,Fujian International Travel Healthcare Center | Huang Z.,Fujian Agriculture and forestry University | And 5 more authors.
Journal of Economic Entomology | Year: 2013

Bacillus thuringiensis (Bt) (Berliner) strain LLP29 produces a crystal protein Cyt1Aa6 toxic to mosquito vectors of human diseases. However, the susceptibility of Culex quinquefasciatus (Say) in the current study was 8.25 times higher than that of Aedes albopictus (Skuse) with this single protein Cyt1Aa6 purified from LLP29. To understand the mechanism of the novel mosquitocidal protein, the binding characteristic of brush border membrane vesicles from the two tested mosquitoes was investigated. Enzyme-linked immunosorbent assay showed that Cyt1Aa6 bound to the two mosquitoes' brush border membrane vesicles. However, the titer of Ae. albopictus was a little higher than that of Cx. quinquefasciatus, with 3.21 and 2.91, respectively. Ligand Western blot analysis showed Cyt1Aa6 toxin specifically bound to the same three proteins (i.e., 68, 54, and 26 kDa) in the two mosquitoes, but one another protein, approximately to 37 kDa, could just be detected in Cx. quinquefasciatus. However, little difference was found in the test of immunohistochemistry. Cyt1Aa6 was detected in the midguts of both mosquitoes with histopathological changes. It would of great importance to the knowledge of the novel toxin against to Cx. quinquefasciatus and Ae. albopictus. © 2013 Entomological Society of America.

Cao J.,Fujian International Travel Healthcare Center | Wei Y.,CAS Research Center for Eco Environmental Sciences | Cheng Y.,Chinese Academy of Inspection and Quarantine
Environmental Science and Pollution Research | Year: 2013

Perfluoroalkyl acids (PFAAs) are carcinogens, and elucidating their DNA binding properties is crucial for understanding PFAA genotoxicity. We have investigated the binding mode and affinity of five PFAAs to seven DNA molecules using fluorescence displacement and molecular docking analysis. DNA conformational changes upon PFAA binding were also examined by circular dichroism (CD). The data revealed that DNA intercalation was the dominant interaction mode of the PFAAs; however, these molecules also bound to grooves. The dissociation constants for the PFAAs ranged between 0.11 and 1,217.14 μM, and between 3.46 and 2,141.21 μM for DNA intercalation and groove binding, respectively. PFAAs that contain longer carbon chains had stronger DNA intercalation affinities. Binding to DNA was stronger for perfluoroalkyl sulfonates than for perfluorcarboxyl acids that contain the same number of carbons. This observation is postulated to arise from the presence of more fluorine and oxygen atoms in perfluoroalkyl sulfonates acting as hydrogen bond donors that facilitate stronger DNA intercalation. The binding of the PFAAs to DNA showed some CT-DNA sequence selectivity. Molecular docking analysis confirmed the DNA binding mode and affinities of the PFAAs. CD analysis revealed that the PFAAs weakened DNA base stacking and loosened DNA helicity. The present study has improved our understanding of the formation of PFAA-DNA adducts. © 2013 Springer-Verlag Berlin Heidelberg.

Zhong G.,Fujian Medical University | Lan R.,Fujian Medical University | Zhang W.,Fujian Medical University | Fu F.,The County Hospital of Anxi | And 7 more authors.
International Journal of Nanomedicine | Year: 2015

A sensitive electrochemical immunosensor was developed for detection of alpha-fetoprotein (AFP) based on a three-dimensional nanostructure gold electrode using a facile, rapid, “green” square-wave oxidation-reduction cycle technique. The resulting three-dimensional gold nanocomposites were characterized by scanning electron microscopy and cyclic voltammetry. A “sandwich-type” detection strategy using an electrochemical immunosensor was employed. Under optimal conditions, a good linear relationship between the current response signal and the AFP concentrations was observed in the range of 10–50 ng/mL with a detection limit of 3 pg/mL. This new immunosensor showed a fast amperometric response and high sensitivity and selectivity. It was successfully used to determine AFP in a human serum sample with a relative standard deviation of <5% (n=5). The proposed immunosensor represents a significant step toward practical application in clinical diagnosis and monitoring of prognosis. © 2015 Zhong et al.

Wang F.,Fujian Agriculture and forestry University | Wang F.,Fujian International Travel Healthcare Center | Huang E.,Fujian International Travel Healthcare Center | Zhang L.,Fujian Agriculture and forestry University | And 4 more authors.
Oriental Insects | Year: 2014

Two new species of the dipteran genus Dasyhelea Kieffer, viz. Dasyhelea silvatica sp.nov., Wang, Zhang and Yu and Dasyhelea arrhena sp.nov., Wang, Zhang and Yu, are described from China. The type specimens are deposited with the Medical Entomology Collection Gallery, Beijing, China. © 2015 Taylor & Francis.

Liu A.-L.,Fujian Medical University | Wang K.,Fujian Medical University | Chen W.,Fujian Medical University | Gao F.,Fujian Medical University | And 4 more authors.
Electrochimica Acta | Year: 2012

A facile and sensitive electrochemical method was introduced to simultaneously determine acetaminophen (AAP) and its degradation product (p-aminophenol, PAP) on ultrathin poly (calconcarboxylic acid, CCA) film modified glassy carbon electrode (GCE). The results exhibited the poly-CCA modified GCE remarkably increased the peak currents of AAP and PAP oxidation. The poly-CCA modified GCE was utilized to electrochemically detect the individual or the mixture of AAP and PAP by cyclic voltammetry (CV) or differential pulse voltammetry (DPV) methods. Factors or conditions can make a distinct contribution to their shapes and separation of these two analytes. Results showed that under the optimal condition, the peak potential of AAP and PAP was well separated and the peak potential difference between them was reached to 250 mV at a scan rate of 100 mV s -1 in DPV. The peak currents of AAP and PAP oxidation increase linearly with the respective concentrations. This method has been successfully applied to the detection of AAP and trace PAP in acetaminophen tablets. This assay has also been successfully applied in the pharmacokinetic study of AAP in vivo through intragastric administration of acetaminophen tablets to SD-rats. © 2011 Elsevier Ltd. All rights reserved.

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