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Zhang L.,Fujian Agriculture and forestry University | Zhang Q.,Fujian Agriculture and forestry University | Huang E.,Fujian International Travel Health Care Center | Li M.,Fujian Agriculture and forestry University | And 5 more authors.
Journal of the American Mosquito Control Association | Year: 2012

We compared the differential display of Aedes albopictus cells, both resistant and susceptible to Bacillus thuringiensis israelensis (Bti), using differentially displayed reverse transcription polymerase chain reaction. We found 1 band about 200 base pairs long. After cloning and sequencing, the differentially expressed gene was similar to some partial messenger ribonucleic acid of Ae. aegypti, Culex quinquefasciatus, and Anopheles gambiae, rather than Ae. albopictus. This will be of some value for clarifying the mechanism of mosquito resistance to Bti products. © 2012 by The American Mosquito Control Association, Inc. Source

Zhang L.,Fujian Agriculture and forestry University | Peng Y.,Fujian Agriculture and forestry University | Wu S.,Fujian Agriculture and forestry University | Sun L.,Fujian Agriculture and forestry University | And 6 more authors.
Current Microbiology | Year: 2012

To explain the association of Bacillus thuringiensis (Bt) with animal feces, an ecological analysis in chickens was conducted by introducing a cry strain marked by production of green fluorescent protein (GFP). After feeding with the tagged Bt strains, the feces of the tested chickens were collected at different times, isolated, and the morphology of Bt was observed. It was shown that Bt strain HD-73GFP in spore form could be isolated from feces of chickens for a period of 13 d, and then it disappeared thereafter. Bt could be detected only up to day 4 (but not thereafter), when chickens were fed with vegetative cells of HD-73GFP. To confirm the source of newly isolated strains, the gfp gene was examined by polymerase chain reaction (PCR), which showed that all the isolated strains harbored the marker gene. Recent data from isolation and PCR had suggested that fecal Bt strains had originated from food. Chicken tissues were thus dissected to isolate Bt strains and to investigate whether Bt could be located in vivo. Bt was located within the duodenum in spore form. Compared to the morphology of the isolated strains at different growth times, the growth rates of all the tested Bt had little changes when passing through the digestive system to the feces. Dissection of the chickens confirmed that Bt was safe for the tested animal. © Springer Science+Business Media, LLC 2012. Source

Zhang L.,Fujian Agriculture and forestry University | Huang E.,Fujian International Travel Health Care Center | Tang B.,Fujian Agriculture and forestry University | Guan X.,Fujian Agriculture and forestry University | Gelbic I.,Academy of Sciences of the Czech Republic
Indian Journal of Experimental Biology | Year: 2012

This study aimed to investigate the resistance mechanism of C6/36 cells to Cyt1Aa6 protein under selection pressure. Receptor binding properties of Cyt1Aa6 toward sensitive and resistant C6/36 cells were investigated. More sensitive cells were detected with goat-anti-rabbit-FITC-labeled antibody, and the quantity of in vitro activated Cyt1Aa6 toxin bound to resistant cells was greatly reduced. Ligand western blot assays showed that disappearance of the 26 kDa protein and weakness of the positive bands of 68 kDa from resistant cells might lead to the resistance of C6/36 cells to Cyt1Aa6 toxin. The resistance of C6/36 cells was detected under selection in vitro-activated Cyt1Aa6 toxin. Receptor binding demonstrated that reduced Cyt1Aa6 bound to resistant cells, which might be closely related to the disappearance and weakness of some proteins. The results presented here are the first to demonstrate that Cyt1Aa protein, a uniquely characteristic toxin, induced resistance at the cellular level. It might be attributed to the change of receptors. Source

Tan Q.-W.,Fujian Agriculture and forestry University | Ouyang M.-A.,Fujian Agriculture and forestry University | Gao B.,Fujian International Travel Health Care Center
Molecules | Year: 2014

Three new ursane triterpenes, 3a,19a-dihydroxy-2-nor-urs-12-en-23,28-dioic acid-23-methyl ester (1), 19a3-dihydroxy-3-oxo-2-nor-urs-12-en-28-oic acid (2), and 2,3-seco-3-methoxy-3,19a3-trihydroxy-urs-12-en-2-al-28-oic acid (3), were isolated from the MeOH extract of the branch barks of Davidia involucrata, together with six known compounds. Their structures were elucidated by means of various spectroscopic analyses. The isolated triterpenes provide important evolutionary and chemotaxonomic knowledge about the monotypic genus Davidia. Five of the identified compounds showed moderate cytotoxicities against the cell proliferation of SGC-7901, MCF-7, and BEL-7404 with IC50 range from 7.26 to 47.41 μM. © 2014 by the authors; licensee MDPI, Basel, Switzerland. Source

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