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Ye Q.,Fujian Institute of Subtropical Botany | Jin X.,Fujian Agriculture and forestry University | Wei S.,Fujian Agriculture and forestry University | Zheng G.,Fujian Agriculture and forestry University | Li X.,Fujian Agriculture and forestry University
Journal of AOAC International | Year: 2016

Subcritical fluid extraction (SFE), as a novel method, was applied to investigate the yield, quality, and sensory evaluation of headspace oil from Jasminum sambac (L.) Aiton in comparison with petroleum ether extraction (PEE). The results indicated that the yield of the headspace oil using SFE was significantly higher (P < 0.05) than when using PEE. SFE contributed to obtaining alcohols and ethers, prevented the thermal reaction of terpenes, and reduced α-caryophyllene and β-caryophyllene in the headspace oil. The contents of linalool (21.90%) and benzyl acetate (16.31%) were higher via SFE than PEE. In addition, the sensory evaluation of SFE was superior to PEE, indicating a fresh, jasmine-like odor and green-yellow color. Thus, SFE is an improved method for obtaining natural headspace oil from jasmine flowers. Source

Zhang J.,Fujian Agriculture and forestry University | Wu H.,Wagga Wagga Agricultural Institute | Wang X.,Fujian Agriculture and forestry University | Huang W.,Fujian Institute of Subtropical Botany
Journal of Horticultural Science and Biotechnology | Year: 2010

Pot experiments were conducted to investigate the subcellular distribution and chemical forms of cadmium (Cd) in order to understand the accumulation, detoxification, and biological toxicity of Cd in strawberry plants. The results showed that the majority of the Cd in leaf cells (82.3%) was deposited in cell walls, followed by organelles (9.7%), and the soluble fraction (8.0%).This distribution pattern remained fairly constant, irrespective of Cd treatment. A similar Cd distribution pattern was found in root cells, although the roots accumulated much higher levels of Cd than the leaves. Sephadex column chromatography showed that the Cd was bound to soluble proteins in the roots.The addition of Cd caused changes in the protein composition of roots, resulting in increased levels of high molecular weight proteins. Sequential solvent extraction showed that the NaCl-extractable fraction contained the highest levels of Cd (89.5%) in leaves as well as in roots. Small amounts of Cd were also present in the extracts prepared using 80% (v/v) ethanol, deionised water, 2% (v/v) acetic acid, or 0.6 M HCl, as well as in the final residues.These results suggest that the preferential accumulation of Cd in roots and in cell walls could provide a mechanism for Cd detoxification. Source

Liu N.-N.,Jilin University | Liu Z.-S.,Jilin University | Lu S.-Y.,Jilin University | Hu P.,Jilin University | And 12 more authors.
Veterinary Immunology and Immunopathology | Year: 2015

Peroxiredoxin 6 (Prdx6), an important antioxidant enzyme that can eliminate reactive oxygen species (ROS) to maintain homeostasis, is a bifunctional protein that possesses the activities of both glutathione peroxidase and phospholipase A2. In this study, a novel full-length Prdx6 cDNA (OaPrdx6) was cloned from Sheep (Ovis aries) using rapid amplification of cDNA ends (RACE). The full-length cDNA of OaPrdx6 was 1753bp containing a 5'-untranslated region (UTR) of 93 bp, a 3'-UTR of 985bp with a poly(A) tail, and an open reading frame (ORF) of 675bp encoding a protein of 224 amino acid residues with a predicted molecular weight of 25.07 kDa. The recombinant protein OaPrdx6 was expressed and purified, and its DNA protection activity was identified. In order to analyze the Prdx6 protein expression in tissues from O. aries, monoclonal antibodies against OaPrdx6 were prepared. Western blotting results indicated that OaPrdx6 protein could be detected in heart, liver, spleen, lung, kidney, stomach, intestine, muscle, lymph node and white blood cells, and the highest expression was found in lung while the lowest expression in muscle. Compared to the normal sheep group, the mRNA transcription level of Prdx6 in buffy coat was up-regulated in the group infected with a virulent field strain of Brucella melitensis, and down-regulated in the group inoculated with a vaccine strain S2 of brucellosis. The results indicated that Prdx6 was likely to be involved in the host immune responses against Brucella infection, and probably regarded as a molecular biomarker for distinguishing between animals infected with virulent Brucella infection and those inoculated with vaccine against brucellosis. © 2015 Elsevier B.V. Source

Zhong L.,Xiamen University | Xie Y.-Z.,Xiamen University | Cao T.-T.,Xiamen University | Wang Z.,Xiamen University | And 6 more authors.
Molecular Neurodegeneration | Year: 2016

Background: Apolipoprotein E (ApoE) is a major cholesterol carrier and plays an important role in maintaining lipid homeostasis both in the periphery and brain. Human APOE gene is polymorphic at two single nucleotides (rs429358 and rs7412) resulting in three different alleles (ε2, ε3 and ε4). ApoE isoforms modulate the risk for a variety of vascular and neurodegenerative diseases; thus, APOE genotyping is crucial for predicting disease risk and designing individualized therapy based on APOE genotype. Results: We have developed an APOE genotyping method that is based on allele-specific PCR methodology adapted to Real Time PCR monitored by TaqMan probe. Rather than using TaqMan probes specific for the two polymorphic sites, only one TaqMan probe is used as the polymorphic alleles are recognized by site-specific PCR primers. Each genotyping assay can be completed within 90 minutes and is applicable to high-throughput analysis. Using this protocol, we genotyped a total of 1158 human DNA samples and obtained a 100 % concordance with the APOE genotype determined by sequencing analysis. Conclusion: The APOE genotyping assay we have developed is accurate and cost-effective. In addition, our assay can readily be applied to genotyping large sample numbers. Therefore, our APOE genotyping method can be used for assessing the risk for a variety of vascular and neurodegenerative diseases that have been reported to be associated with APOE polymorphism. © 2016 Zhong et al. Source

Gu Y.,Xiamen University | Gu Y.,Fujian Institute of Subtropical Botany | Xiao L.,Xiamen University | Ming Y.,Research and Development Center for Medicine Plants and Plant Drugs | And 2 more authors.
International Journal of Oncology | Year: 2016

Corilagin is a natural plant polyphenol tannic acid with antitumor, anti-inflammatory, and anti-oxidative properties. However, the mechanisms of its actions are largely unknown. Our group reported that corilagin could induce cell inhibition in human breast cancer cell line MCF-7 and human liver hepatocellular carcinoma cell lines HepG2. We report here that corilagin inhibits cholangiocarcinoma (CCA) development through regulating Notch signaling pathway. We found that, in vitro, corilagin inhibited CCA cell proliferation, migration and invasion, promoted CCA cell apoptosis, and inhibited Notch1 and Notch signaling pathway protein expression. Co-immunoprecipitation was used to establish Notch intracellular domain (NICD) interaction with MAML1 and P300 in CCA. Importantly, corilagin reduced Hes1 mRNA level through inhibiting Hes1 promoter activity. In nude mice, corilagin inhibited CCA growth and repressed the expression of Notch1 and mTOR. These results indicate that corilagin may control CCA cell growth by downregulating the expression of Notch1. Therefore, our findings suggest that corilagin may have the potential to become a new therapeutic drug for human CCA. Source

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