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Xu X.-X.,Laboratory of Tropical Microbial Resource | Qu Z.,Laboratory of Tropical Microbial Resource | Wang H.,Fujian Institute of Microbiology | Lin H.-P.,Laboratory of Tropical Microbial Resource | And 4 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2011

A Gram-reaction-positive, non-motile actinobacterium, designated strain 210121 T, was isolated from rhizosphere soil of the mangrove fern Acrostichum speciosum. 16S rRNA gene sequence analysis showed that the isolate belonged to the genus Asanoa. DNA-DNA relatedness values between strain 210121 T and the type strains of the three recognized species of the genus Asanoa were below the 70% threshold recommended for distinguishing bacterial genomic species. The novel isolate contained glutamic acid, glycine, alanine and meso-A2pm as cell-wall amino acids, indicating peptidoglycan type A1c. The characteristic whole-cell sugars were xylose, ribose, glucose and mannose. The predominant menaquinones were MK-9(H 4), MK-9(H 6) and MK-9(H 8). The major fatty acids were iso-C 16: 0 (30.9%), C17: 0 (23.0%), anteiso-C 15: 0 (14.9%) and iso-C 15: 0 (12.3%). The phospholipid profile comprised phosphatidylethanolamine, phosphatidylinositol mannosides and phospholipids of unknown structure containing glucosamine. The G+C content of the DNA was 70.3 mol%. On the basis of the phenotypic and genotypic data, strain 210121 T (=CGMCC 4.5593 T =DSM 45427 T) represents a novel species of the genus Asanoa, for which the name Asanoa hainanensis sp. nov., is proposed. An emended description of the genus Asanoa is also proposed. © 2011 IUMS. Source

Xu X.-X.,Chinese Academy of Sciences | Xu X.-X.,Qiongzhou University | Wang H.-L.,Chinese Academy of Sciences | Wang H.-L.,Fujian Institute of Microbiology | And 7 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2012

Strain 211020T was isolated from rhizosphere soil of Excoecaria agallocha in a mangrove in Hainan, China. The strain produced longitudinal pair spores branching from aerial hyphae. 16S rRNA gene sequence analysis showed that the isolate belonged to the genus Microbispora, exhibiting the highest 16S rRNA gene sequence similarity (98.75 %) to Microbispora corallina JCM 10267T with a low DNA-DNA relatedness value (13±0.6 %). The isolate contained mesodiaminopimelic acid as the diagnostic diamino acid but madurose was not detected. The predominant menaquinones were MK-9(H4), MK-9(H2) and MK-9(H0), and the major fatty acids were iso-C16: 0, iso-C15: 0 and C17: 0. The phospholipid profile of strain 211020T comprised phosphatidylinositol mannoside, phosphatidylethanolamine, diphosphatidylglycerol and phospholipids of unknown structure containing glucosamine. The DNA G+C content was 70.8 mol%. On the basis of phenotypic and genotypic data, strain 211020T can be distinguished as a novel species of the genus Microbispora, for which the name Microbispora hainanensis sp. nov., is proposed. The type strain is 211020T (=CGMCC 4.5595T=DSM 45428T).© G 2012 IUMS. Source

Jiang H.,Fujian Institute of Microbiology
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2010

OBJECTIVE: The aim of this study was to investigate actinobacterial diversity in Chilean marine sediments. METHODS: Actinobacterial diversity in these sediments was investigated by selective isolation method, culture-independent method and phylogenetic analysis based on 16S rRNA gene sequences. Six selective media were used to isolate actinomycetes from sediment samples. The primers for the class Actinobacteria were used for Actinobacterial 16S rRNA gene amplification and then a clone library was constructed for the sediment sample btt. Twenty-two strains with different culture characteristics and 59 clones from sample btt were selected for 16S rRNA gene sequences analysis. To determine requirement for seawater each strain was grown on oatmeal agar prepared with deionized water and with seawater, respectively. Strains were screened for antibiotic activity against bacteria and fungi. RESULTS: In total 328 actinomycetes were obtained. Twenty-two strains which were selected belonged to Streptomyces, Micromonospora, Polymorphospora, Aeromicrobium and Brachybacterium. Fifty-nine clones (40 OTUs) were sequenced, and 60% OTUs belonged to Actinobacteridae, Acidimicrobidae and Rubrobacteridae. The other 40% OTUs, which formed several distinct clades in phylogenetic tree among phylum Actinobacteria may represent new taxonomical groups. 50% of the 47 sea water dependant strains and nineteen strains out of the above 22 strains exhibited antimicrobial activity. CONCLUSION: There was abundant actinobacterial diversity in the marine sediments of Chile, and the result implied that there were large numbers of unknown actinobacterial groups in the sediments. Actinomycetes from Chilean marine sediments had the potential of producing bioactive secondary metabolites. Source

Shen Y.,Fuzhou University | Zhu W.,Fuzhou University | Chen C.,Fuzhou University | Nie Y.,Fujian Institute of Microbiology | Lin X.,Fuzhou University
Bioprocess and Biosystems Engineering | Year: 2016

The objective of this study was to investigate the fundamental question of biofilm formation. First, a drum biofilm reactor was introduced. The drums were coated with three porous substrates (cotton rope, canvas, and spandex), respectively. The relationships among the substrate, extracellular polymeric substances (EPS), and adhesion ratio were analyzed. Second, a plate biofilm reactor (PBR) was applied by replacing the drum with multiple parallel vertical plates to increase the surface area. The plates were coated with porous substrates on each side, and the nutrients were delivered to the cells by diffusion. The influence of nitrogen source and concentration on compositions of EPS and biofilm formation was analyzed using PBR under sunlight. The results indicated that both substrate and nitrogen were critical on the EPS compositions and biofilm formation. Under the optimal condition (glycine with concentration of 1 g l−1 and substrate of canvas), the maximum biofilm productivity of 54.46 g m−2 d−1 with adhesion ratio of 84.4 % was achieved. © 2016 Springer-Verlag Berlin Heidelberg Source

Zhao X.,Ludwig Maximilians University of Munich | Zhao X.,Fujian Institute of Microbiology | Pabel J.,Ludwig Maximilians University of Munich | Hofner G.C.,Ludwig Maximilians University of Munich | Wanner K.T.,Ludwig Maximilians University of Munich
Bioorganic and Medicinal Chemistry | Year: 2013

A series of enantiomerically pure 4-hydroxy-4-(4-methoxyphenyl)-substituted proline and pyrrolidin-2-ylacetic acid derivatives have been synthesized starting from the respective N-protected 4-hydroxy derivatives via oxidation to the corresponding 4-oxo compounds, subsequent addition of organometallic reagents, final hydrolysis and deprotection. The major diastereoisomers obtained by the addition of the Grignard reagents were found to have opposite stereoconfigurations depending on whether cerium trichloride was present or absent as an additive. The final compounds were evaluated for their capability to inhibit the GABA transport proteins GAT1 and GAT3. 4-Hydroxyproline derivatives substituted with a tris(4-methoxyphenyl)methyloxyethyl residue at the nitrogen and a 4-methoxyphenyl group in 4-position showed, with the exception of the (2R,4R)-diastereomer, an improved inhibition at GAT3 compared to the derivatives missing the 4-methoxyphenyl group in 4-position. This may imply that an appropriate lipophilic group at the C-4 position of the proline moiety is beneficial for potent inhibition at GAT3. © 2012 Elsevier Ltd. All rights reserved. Source

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