Fujian Institute of Education

Fuzhou, China

Fujian Institute of Education

Fuzhou, China
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Wu J.-G.,Fujian University of Traditional Chinese Medicine | Peng W.,Chongqing Medical University | Yi J.,Fujian Institute of Education | Wu Y.-B.,Fujian University of Traditional Chinese Medicine | And 3 more authors.
Journal of Ethnopharmacology | Year: 2014

Ethnopharmacological relevance Leaves of Toona sinensis (A. Juss.) Roem. (TSL), a popular vegetable in China, have anti-inflammatory, antidoting, and worm-killing effects and are used in folk medicine for the treatment of enteritis, dysentery, carbuncles, boils, and especially abdominal tumors. Our aim was to investigate the in vitro antimicrobial activity against Staphylococcus aureus and anticancer property of the essential oil from TSL (TSL-EO), especially the pro-apoptotic effect in SGC-7901. Materials and methods TSL-EO obtained by hydrodistillation was analyzed by GC/MS and was tested in vitro against twenty clinically isolated strains of Staphylococcus aureus (SA 1-20), which were either methicillin-sensitive Staphylococcus aureus (MSSA) or methicillin-resistant Staphylococcus aureus (MRSA) and two standard strains viz. ATCC 25923 and ATCC 43300. The anticancer activity of TSL-EO was evaluated in vitro against HepG2, SGC7901, and HT29 through MTT assay. Moreover, the apoptosis-inducing activity of TSL-EO in SGC7901 cells was determined by Hoechst 33324 staining and flow cytometry methods. Also, the apoptosis-related proteins viz. Bax, Bcl-2 and caspase-3 were detected by western-blotting. Results GC-MS analysis showed that TSL-EO contained a high amount of sesquiterpenes (84.64%), including copaene (8.27%), β-caryophyllene (10.16%), caryophyllene (13.18%) and β-eudesmene (5.06%). TSL-EO inhibited the growth of both MSSA and MRSA, with the lowest MIC values of 0.125 and 1 mg/ml, respectively. Treatment with TSL-EO for 24 h could significantly suppress the viability of three different cancer cell lines (P<0.05). Furthermore, the apoptosis-inducing activity of TSL-EO in SGC7901 cells increased in a dose-dependent manner, potentially resulting from the up-regulated expression of Bax, caspase-3 and down-regulated expression of Bcl-2. Conclusions TSL-EO possessed antibacterial activity against Staphylococcus aureus and significant cytotoxicity against cancer cells and particularly prominent pro-apoptotic activity in SGC7901 cells. These bioactivities were probably due to the high content of sesquiterpenes. Our results suggested that TSL-EO possessed potential health benefits and could serve as a promising natural food addictive. © 2014 Elsevier Ireland Ltd.


Wu Y.-B.,Fujian University of Traditional Chinese Medicine | Zheng L.-J.,Fujian University of Traditional Chinese Medicine | Wu J.-G.,Fujian University of Traditional Chinese Medicine | Chen T.-Q.,Fujian Academy of Agricultural science | And 2 more authors.
International Journal of Molecular Sciences | Year: 2012

The antioxidant activities of ethanolic crude extract (ECE) and its four different solvent sub-fractions (namely, petroleum ether fraction (PEF), ethyl acetate fraction (EAF), n-butanol fraction (BF) and the aqueous fraction (AF) from the receptacles of Nelumbo nucifera Gaertn. (Receptaculum Nelumbinis) were investigated using two in vitro antioxidant assays. BF showed the highest total phenolic content (607.6 mg/g gallic acid equivalents), total flavonoid content (862.7 mg/g rutin equivalents) and total proanthocyanidin content (331.0 mg/g catechin equivalents), accompanied with the highest antioxidant activity compared to other fractions through 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging assays. Five flavonol glycosides, namely hyperoside (1), isoquercitrin (2), quercetin-3-O-β-D-glucuronide (3), isorhamnetin-3-O-β-D-galactoside (4) and syringetin-3-O-β-D-glucoside (5) were isolated from the Receptaculum Nelumbinis. Compounds 2-5 were isolated for the first time from the Receptaculum Nelumbinis. The five isolated flavone glycosides, particularly compounds 1-3, demonstrated significant DPPH and ABTS radical scavenging activity, with IC 50 values of 8.9 ± 0.2, 5.2 ± 0.2, 7.5 ± 0.1 for DPPH and 114.2 ± 1.7, 112.8 ± 0.8, 172.5 ± 0.7 μg/mL for ABTS, respectively. These results suggest that Receptaculum Nelumbinis has strong antioxidant potential and may be potentially used as a safe and inexpensive bioactive source of natural antioxidants. © 2012 by the authors; licensee MDPI, Basel, Switzerland.


Wu Y.-B.,Fujian University of Traditional Chinese Medicine | Zheng L.-J.,Fujian University of Traditional Chinese Medicine | Yi J.,Fujian Institute of Education | Wu J.-G.,Fujian University of Traditional Chinese Medicine | And 2 more authors.
International Journal of Molecular Sciences | Year: 2013

A simple and reliable method of high-performance liquid chromatography with photodiode array detection (HPLC-DAD) was developed to evaluate the quality of Receptaculum Nelumbinis (dried receptacle of Nelumbo nucifera) through establishing chromatographic fingerprint and simultaneous determination of five flavonol glycosides, including hyperoside, isoquercitrin, quercetin-3-O-β-D-glucuronide, isorhamnetin-3-O-β-D-galactoside and syringetin-3-O-β-D-glucoside. In quantitative analysis, the five components showed good regression (R > 0.9998) within linear ranges, and their recoveries were in the range of 98.31%-100.32%. In the chromatographic fingerprint, twelve peaks were selected as the characteristic peaks to assess the similarities of different samples collected from different origins in China according to the State Food and Drug Administration (SFDA) requirements. Furthermore, hierarchical cluster analysis (HCA) was also applied to evaluate the variation of chemical components among different sources of Receptaculum Nelumbinis in China. This study indicated that the combination of quantitative and chromatographic fingerprint analysis can be readily utilized as a quality control method for Receptaculum Nelumbinis and its related traditional Chinese medicinal preparations. © 2013 by the authors; licensee MDPI, Basel, Switzerland.


PubMed | Fujian Institute of Education, Fujian University of Traditional Chinese Medicine and Fujian Academy of Agricultural science
Type: Journal Article | Journal: Pharmaceutical biology | Year: 2016

Ganoderma triterpenoids (GTs) have been recognised as an important bioactive ingredient in Ganoderma Lucidum (Leyss. ex Fr.) Karst. (Polyporaceae), widely used for treating and preventing chronic hepatopathy of various etiologies.The objective of this study is to better understand the hepatoprotective effect of GTs and to enhance their use in food supplement pharmaceutical and medical industries.HepG2 cells were pretreated in the presence or absence of GTs (50, 100 and 200g/ml) for 4h, then exposed with 60mol/L of t-BHP for an additional 4h. The cell viability was evaluated by MTT method. ALT, AST and LDH production in culture medium and intracellular MDA, GSH and SOD levels were determined. Moreover, the total triterpenoid content and chemical constituents in GTs were detected by ultraviolet spectrophotometry and HPLC/Q-TOF-MS, respectively.GTs (50, 100 and 200g/ml) significantly increased the relative cell viability by 4.66, 7.78 and 13.46%, respectively, and reduced the level of ALT by 11.44%, 33.41% and 51.24%, AST by 10.05%, 15.63% and 33.64%, and LDH by 16.03%, 23.4% and 24.07% in culture medium, respectively. GTs could also remarkably decrease the level of MDA and increase the content of GSH and SOD in HepG2 cells. Furthermore, the total triterpenoid content in GTs was 438mg GAAEs/g GTs. And 16 triterpenoids in GTs were identified or tentatively characterised.Our results showed that GTs had potent cytoprotective effect against oxidative damage induced by t-BHP in HepG2 cells, thus suggesting their potential use as liver protectant.


Huang J.,Beijing Institute of Technology | Huang J.,Fujian Institute of Education | Fang H.,Beijing Institute of Technology | Chen J.,Beijing Institute of Technology | And 2 more authors.
Journal of Advanced Computational Intelligence and Intelligent Informatics | Year: 2014

Most previous studies ofmulti-agent systems only considered the first- and second-order dynamics. In this review, we present the major results and progress related to distributed high-order linear multi-agent coordination. We also discuss the current challenges and propose several promising research directions, as well as open problems that demand further investigation.


Wu J.,Fujian University of Traditional Chinese Medicine | Wan Z.,Fujian University of Traditional Chinese Medicine | Yi J.,Fujian Institute of Education | Wu Y.,Fujian University of Traditional Chinese Medicine | Peng W.,Chongqing Medical University
Journal of Natural Medicines | Year: 2013

The aim of this study was to evaluate antioxidant activities and cytotoxicity against human tumor cell lines of extracts from Bidens pilosa Linn. var. radiata Sch. Bip. (BP). Total phenolic and flavonoid contents of the extracts were determined by ultraviolet spectrophotometry. Furthermore, the antioxidant properties of different polarity fractions extracted from BP were evaluated using DPPH and ABTS radical scavenging test and FRAP assay. The ethyl acetate fraction (EE-BP) showed the highest antioxidant activity compared to other fractions. In addition, the anti-proliferative activities of the extracts on four human tumor cells, namely MCF-7, HepG2, MGC 803 and RKO, were investigated by MTT method. The EE-BP displayed the most remarkable anti-proliferative effect against the tumor cells, particularly RKO cell in dose- and time-dependent manner. The antioxidant activities and cytotoxicity correlated highly with the total phenolic and flavonoid contents, respectively. Furthermore, The active ingredient BP-6, namely 5,7,4′-trihydroxy-3, 3′-dimethyl-flavonol, was isolated and purified with the purity above 99.00% and content of 0.15% in EE-BP detected by HPLC, which could significantly inhibit the proliferation of RKO cells with the IC50 value of 6.66 μmol/l. In order to characterize the apoptotic RKO cells, flow cytometry and DNA fragmentation assay were performed. Apoptotic cell numbers increased in a dose-dependent manner after the treatment with different concentrations of EE-BP and BP-6 for 12 and 6 h, respectively. DNA ladders in apoptotic RKO cells could be easily visualized when exposed to 200 μg/ml of the EE-BP for 36 h. Taken together, our work indicated that BP had potentially therapeutic value against colorectal cancer. © 2012 The Japanese Society of Pharmacognosy and Springer.


Wu J.-G.,Fujian University of Traditional Chinese Medicine | Peng W.,Chongqing Medical University | Zeng P.-Y.,Fujian University of Traditional Chinese Medicine | Wu Y.-B.,Fujian University of Traditional Chinese Medicine | And 2 more authors.
Genetics and Molecular Research | Year: 2013

We evaluated the antibacterial activity and cytotoxicity of endophytes isolated from Scapania verrucosa Heeg., which belongs to the liverwort class. A total of 49 endophytic fungi were isolated from S. verrucosa and classified into seven genera and one family in our previous study. In this study, the cytotoxic activity of the endophytes was assessed using the brine shrimp lethality bioassay, seven of which showed potent toxicity against the brine shrimp with 50% lethal concentration values less than 20 μg/mL. T-30 was the most toxic, with a 50% lethal concentration value of 7.15 μg/mL. Moreover, T-27 exhibited the strongest antibacterial activity against Staphylococcus aureus, with minimal inhibitory concentrations below 0.25 and 4 mg/mL, which can inhibit the growth of two standard strains - ATCC 25923 (methicillin-sensitive S. aureus) and ATCC 43300 (methicillin-resistant S. aureus) - in a time-dependent manner, respectively. These results suggest that endophytes in S. verrucosa are the sources for the production of natural bioactive products and thus warrant further investigation. © FUNPEC-RP.


Chen L.,Fujian Institute of Education | Xie X.,Ningde Teachers College
Nonlinear Analysis: Real World Applications | Year: 2011

In this paper, we consider an N-species cooperation system with continuous time delays and feedback controls. By applying a new integral inequality, we obtain a set of sufficient conditions which ensure the permanence of the system. Our result shows that feedback control variables have no influence on the permanence of the system, thus improving the corresponding results of Chen, Liao and Huang [F.D. Chen, X.Y. Liao, Z.K. Huang, The dynamic behavior of N-species cooperation system with continuous time delays and feedback controls, Applied Mathematics and Computation 181 (2) (2006) 803815]. © 2010 Published by Elsevier Ltd. All rights reserved.


Chen L.,Fuzhou University | Chen L.,Fujian Institute of Education
Nonlinear Analysis: Real World Applications | Year: 2010

By using the method of coincidence degree, some conditions are obtained for the existence of positive periodic solution of a nonlinear integro-differential prey-competition impulsive model with infinite delays. © 2009 Elsevier Ltd. All rights reserved.


PubMed | Fujian Institute of Education and Fujian University of Traditional Chinese Medicine
Type: Journal Article | Journal: Journal of separation science | Year: 2016

The high-performance liquid chromatography fingerprint method is a simple and reliable technique to evaluate the quality of leaves of Magnolia officinalis Rehd.et Wils. var. biloba Rehd.et Wils. We used the following bioactive phenolic constituents as reference compounds: rutin, afzelin, hyperoside, isoquercitrin, quercetin-3-O--l-rhamnoside, honokiol and magnolol. The conditions of an Agilent 1200 HPLC were: YMC-Pack-ODS-AQ column (250 4.6 mm id S-5 m, 12 nm), mobile phase acetonitrile and 0.2% phosphoric acid in a gradient elute mode, flow rate 1.0 mL/min, detection wavelength 280 nm and column temperature 30C. The analytical method was validated in terms of linearity, stability, repeatability, precision and recovery tests. While performing fingerprint analysis, we identified 11 peaks as characteristic peaks and assessed the similarities of 17 samples collected from different geological regions of China. The peak areas were used to evaluate the variation in the chemical composition of the tested samples. For this purpose, we performed hierarchical cluster analysis of the peak areas. Our results indicate that simultaneous determination of multiple ingredients could be done through chromatographic fingerprint analysis. Therefore, this high-performance liquid chromatography fingerprint method was readily utilized to evaluate the quality of leaves of M. officinalis var.biloba, which are used in several traditional herbal preparations.

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