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Wang Q.,Fujian Agriculture and forestry University | Huang Y.,Fujian Agriculture and forestry University | Wu B.,Fujian Agriculture and forestry University | Mei J.,Fujian Agriculture and forestry University | And 2 more authors.
Journal of Traditional Chinese Medicine | Year: 2014

OBJECTIVE: To investigate how the pretreatment of mice with Ganoderma spores affected the apoptosis of their splenic lymphocytes induced by dexamethasone after 19 days treatment. METHODS: Sixty Kunming mice were randomly divided into six groups: blank control groupdrenched with normal saline; a drug control group drenched with 150 mg/mL Ganoderma spores; a model group treated with saline; a low dose group with 50 mg/mL Ganoderma spores; a moderate dose group with 100 mg/mL Ganoderma spores; and a high dose group with 150 mg/mL Ganoderma spores. The effect of Ganoderma spores on apoptosis in spleen lymphocytes was analyzed. All groups were treated for 19 days. On day 20, the model group and the 3 treatment groups were intraperitoneally injected dexamethasone to induce apoptosis. Splenic index and apoptosis indes were employed to measure cell apoptosis. RESULTS: The results showed that Ganoderma spores reduced the splenic index to different degrees in each group and the best effect was seen in the high dose group (P<0.05). Terminal dexynucleotidyl transferase (TdT)-mediated 2'-Deoxyuridine 5'-Triphosphate nick end labeling staining revealed that the apoptotic index in all groups administered Ganoderma spores differed significantly from the model group, and a dose-response was observed. Flow cytometric analysis indicated that spleen lymphocyte apoptosis in the model group was extensive. Each dose of Ganoderma spores inhibited dexamethasone-induced apoptosis in spleen lymphocytes, and a dose-response was observed as well. The highest dose of Ganoderma spores decreased Malondialdehyde content in serum induced by dexamethasone (P<0.05). CONCLUSION: The findings imply that the pretreatment of the mice with Ganoderma spores could reduce the apoptosis rate induced by dexamethasone in their splenic lymphocytes. © 2014 JTCM. All rights reserved. Source


Wu C.M.,China Pharmaceutical University | Wu C.M.,Fujian Institute for Drug Control | Zhou L.M.,Fujian Institute for Drug Control | Chai Y.F.,China Pharmaceutical University | And 2 more authors.
Chinese Chemical Letters | Year: 2010

Three new sesquiterpene alkaloids, 1-desacetylwilforgine (1), 1-desacetylwilforine (2), and 9'-hydroxy-2-nicotinoylwilforine (3) were isolated from the roots of Tripterygium wilfordii Hook f., along with six known alkaloids. Their structures were established on the basis of spectral analysis. © 2010 Guo Rong Fan. Source


Zhou L.M.,Fujian Institute for Drug Control | Zhou L.M.,Kunming Biovalley Material Medical Research Institute | Du J.,Kunming Biovalley Material Medical Research Institute | Wu C.M.,Fujian Institute for Drug Control
Chinese Chemical Letters | Year: 2010

A new triterpenoid 3,4,6-trihydroxy-2-oxo-1(10), 3,5,7-tetraen-23, 24-nor-D: A-friedooleana-29-oic acid, as well as twelve known terpenes were isolated from the roots of Tripterygium wilfordii. Its structure was established on the basis of spectroscopic methods. © 2010 Jiang Du. Source

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