Lin Y.,Fujian Health College |
Shi Z.,Duquesne University |
Wildfong P.L.D.,Duquesne University
Journal of Pharmaceutical and Biomedical Analysis | Year: 2010
A method for measuring the thermal conductivity (k) of small molecule organic solid materials using modulated differential scanning calorimetry (MDSC) is demonstrated. Sample preparation required powder consolidation, unavoidably introducing air voids into compacts. Supporting equations for the technique were modified to include a porosity term (ε), and the theoretical quadratic relationship between k and ε was confirmed by experimental measurements for 18 representative materials. Zero-porosity extrapolation was used to approximate values of "true" thermal conductivity for non-porous solids (k ε=0). Zero-porosity-extrapolated values ranged from 0.1273 W/(K m) to 0.3472 W/(K m) for all materials, consistent with expected values of k for non-porous organic polymers. © 2009 Elsevier B.V. All rights reserved.
Lin Z.,Nanjing University |
Lin Z.,Fujian University of Traditional Chinese Medicine |
Fang Y.,Fujian University of Traditional Chinese Medicine |
Huang A.,Fujian University of Traditional Chinese Medicine |
And 4 more authors.
Pharmaceutical Biology | Year: 2014
Context: Sedum aizoon L. (Crassulaceae) (SA) is widely used to treat various hemorrhages in folk medicine. However, its hemostatic constituents are not yet clear.Objective: The chemical constituents of EtOAc fraction from SA and their hemostatic activity were investigated to provide a basis for the application in folk use.Materials and methods: The chemical constituents were isolated from the aerial parts of SA by column chromatography and identified by IR, MS, and NMR, then tested for hemostatic activity using the capillary method and coagulation assays including blood clotting time in vivo, and prothrombin time (PT), activated partial thromboplastin time (APTT), and thrombin time (TT) in vitro at concentrations of 300.0, 100.0, and 30.0g/mL.Results: Eleven compounds were identified as p-hydroxybenzoic acid (1), gallic acid (2), protocatechuic acid (3), vallinic acid (4), thymine (5), caffeic acid (6), 5,7-dihydroxy chromone (7), pyrogallol (8), quercetin (9), kaempferol (10), and luteolin (11). This is the first report of compounds 3-8 being isolated from this plant. Compounds 2 (300.0 and 100.0g/mL), 4 (100.0g/mL), and 11 (100.0 and 30.0g/mL) significantly reduced the clotting time (p<0.01) with inhibition rates of 34.7, 24.5, 30.3, 25.9, and 36.6%, respectively. For further mechanism study, they also reduced PT (3.5, 2.5, 3.5, 3.5, and 3.8%, respectively), APTT (4.5, 3.3, 11.4, 8.5, and 11.1%, respectively), and TT (20.3, 3.8, 7.6, 6.1, and 10.3%, respectively).Discussion and conclusion: SA produced hemostatic activity possibly related to the presence of gallic acid, vallinic acid, and luteolin, which may be potent candidates of hemostatic drug. © 2014 Informa Healthcare USA, Inc. All rights reserved.
Continuous cadmium exposure from weaning to maturity induces downregulation of ovarian follicle development-related SCF/c-kit gene expression and the corresponding changes of DNA methylation/microRNA pattern
Weng S.,Fujian Medical University |
Wang W.,Fujian Medical University |
Li Y.,Fujian Medical University |
Li H.,Fujian Health College |
And 5 more authors.
Toxicology Letters | Year: 2014
Cadmium (Cd) impairs ovary structure and function in mature animals. However, the influence of Cd on follicle development from weaning to maturity is obscure. In the current study, 21-day-old Wistar rats were administered Cd chloride at doses of 0, 0.5, 2.0 and 8.0. mg/kg body weight once a day for eight weeks by gavage. After administration, a significant decrease in ovarian wet weight, ovarian/body weight ratios, and primordial follicles, in addition to an increase in atresic follicles, were observed. Transmission electron microscopy and TUNEL assay confirmed the increase of follicle apoptosis as Cd concentration increased. Real-time quantitative PCR and Western blotting showed a significantly decreased expression of follicle development-related factors, stem cell factor (SCF) and c-kit. Bisulfite sequencing suggested that the total methylation percentages of SCF/c-kit promoter region were not obvious change after Cd exposure. Real-time quantitative PCR revealed a significantly increased expression of miR-193, miR-221 and miR-222, which regulate c-kit, in the 2.0. mg/kg and 8.0. mg/kg treatment groups. Overall, this study proved that Cd administration from weaning to maturity could damage follicle development, suggesting that SCF/c-kit might play an important role in this effect. In addition, microRNAs might play a role in c-kit protein downregulation. © 2014 Elsevier Ireland Ltd.
Li Y.,Fuzhou University |
Li Y.,Fujian Health College |
Wu H.,Fuzhou University |
Guo L.,Fuzhou University |
And 3 more authors.
Food Chemistry | Year: 2012
The determination of citrinin (CIT) by a microsphere-based flow cytometric immunoassay (MFCI) has been developed. In the method, the carboxyl-modified microspheres were conjugated with CIT-Ovalbumin (OVA) antigen. CIT competed with the CIT-OVA antigen on the surface of the microspheres for the anti-CIT McAb. Under the optimised conditions, IC50 value was 1.0 ng/mL and the limit of detection reached 0.005 ng/mL. The cross-reactivity was less than 0.01% against each of the four mycotoxins such as aflatoxin B1 (AFB 1), ochratoxin A (OTA), zearalenone (ZEA), deoxynilvalenol (DON). In the work, the MFCI could accurately determine CIT in the real red yeast rice. The systematic error was low with the coefficient of variation (CV) from 5.24% to 8.16% by the MFCI. The mean recovery of CIT from artificially contaminated red yeast rice was from 89% to 94%, with CV from 7.2% to 8.7%. The experimental data showed that the precision, sensitivity and specificity of the developed MFCI method for the determination of CIT were satisfactory. © 2012 Elsevier Ltd. All rights reserved.
Sun L.,Fuzhou University |
Lin D.,Fujian Health College |
Lin G.,Fujian Health College |
Wang L.,Fuzhou University |
Lin Z.,Fuzhou University
Analytical Methods | Year: 2015
A facile strategy based on the synergistic effect of molecular imprinting and boronate affinity was proposed for glycoprotein imprinting. Polydopamine (PDA)-coated boronic acid-functionalized molecularly imprinted silica nanoparticles (MIPs) were prepared by the "thiol-ene" click reaction using SiO2 as the core, 3-acrylamidophenyl boronic acid (AAPBA) as the functional monomer, and horseradish peroxidase (HRP) as the glycoprotein template. A well defined core-shell structure of MIPs was obtained after self-polymerization of dopamine (DA) on the surface of HRP-immobilized silica nanoparticles (NPs). The polymerization conditions and adsorption behavior were investigated in detail in order to obtain the highest selectivity and binding capacity. Under the optimized conditions, the HRP-MIPs showed higher binding affinity towards HRP than non-imprinted nanoparticles (NIPs), and the corresponding adsorption capacity (Q) and imprinted factor (α) reached 0.58 μmol g-1 and 2.6, respectively. The specificity for HRP recognition was evaluated with a competitive experiment, and the results indicated that the HRP-MIPs had higher selectivity for the template. The good features of the HRP-MIPs facilitated selective isolation and enrichment of trace HRP from human serum. In addition, the stability and regeneration were also investigated, which indicated that the HRP-MIPs had excellent reusability. © 2015 The Royal Society of Chemistry.
Tian J.,Fujian Medical University |
Jia L.N.,Fujian Health College |
Cheng Z.C.,Fujian Tumor Hospital
Current Oncology | Year: 2015
Aim We aimed to assess the relationships of patient education with the severity of treatment-induced side effects, daily calorie and protein intake, psychological status, and performance status in patients with lung cancer. Methods The study patients were divided into an intervention (n = 62) and a control group (n = 110). The patients in the intervention group were provided with information about treatment, diet, and rehabilitation during chemotherapy. The patients in the control group were not specially provided with that information. Results We observed significant differences between the intervention and control groups with respect to low daily protein intake (54.84% vs. 70.00%, p = 0.046), prevalence of depression (51.61% vs. 70.91%, p = 0.011), prevalence of severe side effects of treatment (14.52% vs. 37.27%, p = 0.002), and good performance status (75.81% vs. 55.45%, p = 0.008). Conclusions Our results suggest that educating patients about cancer treatment and rehabilitation can lead to increased protein intake, a lower prevalence of depression, lesser side effects from cancer treatments, and improved performance status. © 2015 Multimed Inc.
Li Q.,University of Houston |
Fan F.,University of Houston |
Fan F.,Fujian Health College |
Kwak H.R.,University of Houston |
Yan J.,University of Houston
Journal of General Physiology | Year: 2015
Large conductance Ca2+-and voltage-activated potassium (BK) channels are comprised of pore-forming γ subunits and various regulatory auxiliary subunits. The BK channel auxiliary γ (BKγ) subunits are a newly identified class of proteins containing an extracellular leucine-rich repeat domain (LRRD), a single transmembrane (TM) segment, and a short cytoplasmic C-terminal tail (C-tail). Although each of the four BKγ proteins shifts the voltage dependence of BK channel activation in a hyperpolarizing direction, they show markedly different efficacies, mediating shifts over a range of 15-145 mV. Analyses of chimeric BKγ subunits created by swapping individual structural elements, and of BKγ deletion and substitution mutants, revealed that differential modulation of BK gating by the four BKγ subunits depends on a small region consisting of the TM segment and the adjacent intracellular cluster of positively charged amino acids. The γ1 and γ2 TM segments contributed approximately γ100 mV, and the γ1 and γ3 C-tails contributed approximately γ40 mV, to shifting the voltage dependence of BK channel activation, whereas the γ3 and γ4 TM segments and the γ2 and γ4 C-tails contributed much less. The large extracellular LRRDs were mainly functionally interchangeable, although the γ1 LRRD was slightly less effective at enhancing (or slightly more effective at attenuating) the shift in BK channel voltage-dependent gating toward hyperpolarizing potentials than those of the other BKγ subunits. Analysis of mutated BKγ subunits revealed that juxta-membrane clusters of positively charged amino acids determine the functions of the γ1 and γ3 C-tails. Therefore, the modulatory functions of BKγ subunits are coarse- and fine-tuned, respectively, through variations in their TM segments and in the adjacent intracellular positively charged regions. Our results suggest that BK channel modulation by auxiliary γ subunits depends on intra- and/or juxta-membrane mechanisms. © 2015 Li et al.
Yang C.,Fujian Agriculture and forestry University |
Ma J.,Wuhan University |
Zhang M.,Fujian Health College |
Zheng S.,China Three Gorges University |
Tian X.,Wuhan University
Infrared Physics and Technology | Year: 2014
In this paper, we proposed a new robust infrared small target detector that is more suitable for complex background with low signal-to-clutter ratio. The original image is decomposed into sub-bands in different orientations by using the directional second-order directional derivative (DSODD) filters deduced from the facet model. The multiscale facet model (MFM) analysis is developed by using a series of multiscale DSODD filters, which are obtained by filling zeros in the basic DSODD filter. Based on MFM, an MFM matrix is constructed, and the normalized determinant of this matrix is then defined as the target measure. The corresponding multiscale correlations of the target measures are computed to enhance the target signal and suppressing the background clutter. The experimental results on a set of real infrared images demonstrate that the proposed approach is effective and is superior to the traditional small target detection methods in terms of the pertained quantitative detection evaluation indexes, such as the signal-to-clutter ratio gain and the background suppression factor. © 2014 Elsevier B.V. All rights reserved.
Zhao B.,Fuzhou University |
Zhuang N.,Fuzhou University |
Li T.,Shandong University |
Guo F.,Fuzhou University |
And 3 more authors.
Journal of Crystal Growth | Year: 2011
A new Nd: Lu0.141Y0.855VO4 mixed laser crystal had been successfully grown by the Czochralski method. The reason of the crystal boule appearing additionally yellowish at the bottom was discussed. The X-ray powder diffraction analysis showed that the as-grown mixed crystal possesses the ZrSiO4 structure. The effective segregation coefficients of Nd3 and Lu3 ions in the crystal were measured to be about 0.51 and 0.76, respectively. The room temperature fluorescence spectrum of the mixed crystal showed that the FWHM (full-width at half-maximum) of the 1064.9 nm fluorescence band was 5.1 nm, broader than that of Nd: YVO4. A continuous-wave (CW) laser output was achieved with the Nd: Lu0.141Y0.855VO4 crystal at 1.06 μm using laser diode pumping. The maximum CW output power of 1.7 W was obtained with the pump power of 7.6 W, higher than the 1.38 W achieved with the 0.5 at% Nd: LuVO4 crystal under the same experimental conditions. All the results above show that the Nd: Lu0.141Y0.855VO 4 crystal is a promising laser material. © 2011 Elsevier B.V. All Rights Reserved.
Ni F.,Fujian Health College |
Ni F.,Beth Israel Deaconess Medical Center |
Gong Y.,Beth Israel Deaconess Medical Center |
Li L.,Beth Israel Deaconess Medical Center |
And 2 more authors.
PLoS ONE | Year: 2012
The objective of this study was to evaluate the chemopreventive effect of a novel flavonoid, ampelopsin (AMP) on the growth and metastasis of prostate cancer cells. AMP showed the more potent activity in inhibiting the proliferation of androgen-sensitive LNCaP and, to less extent, androgen-independent PC-3 human prostate cancer cell lines in vitro, primarily by induction of apoptosis associated with down-regulation of bcl-2. On the other hand, AMP showed much less activity in inhibiting the proliferation of normal prostate epithelial cells than that of prostate cancer cell lines. AMP also inhibited the migration and invasion of PC-3 cells in vitro associated with down-regulation of CXCR4 expression. In the animal study using an orthotopic prostate tumor model, AMP (150 and 300 mg/kg body weight) inhibited the growth of PC-3 tumors and lymph node and lung metastases in a dose-dependent manner. Compared to the control mice, mice treated with AMP at 300 mg/kg BW had reduced final tumor weight by 49.2% (P<0.05), lymph node metastases by 54.5% (P = 0.3) and lung metastases by 93% (P<0.05), but had no apparent alteration on food intake or body weight. The in vivo anti-growth and anti-metastasis activities of AMP were associated with induction of apoptosis and inhibition of proliferation of prostate cancer cells, reduction of prostate tumor angiogenesis, and reduction of CXCR4 expression. Our results provide supporting evidence to warrant further investigation to develop AMP as a novel efficacious and safe candidate agent against progression and metastasis of prostate cancer. © 2012 Ni et al.