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Mu Y.,State Oceanic Administration | Wan X.,State Oceanic Administration | Lin K.,Fujian Fisheries Research Institute | Ao J.,State Oceanic Administration | Chen X.,State Oceanic Administration
Fish Physiology and Biochemistry | Year: 2013

In the present study, we examined the liver protein profiles of the large yellow croaker (Pseudosciaena crocea) exposed to polyriboinosinic:polyribocytidylic acid [poly(I:C)], a viral mimic, using the differential proteomic approach. Sixteen altered protein spots were identified by matrix-assisted laser desorption ionization time of flight mass spectrometry or matrix-assisted laser desorption ionization time of flight/time of flight mass spectrometry, including eight upregulated proteins and eight downregulated proteins. These altered host proteins were classified into six categories based on their biological function: cellular process, metabolic process, biological regulation, binding, and catabolic process, highlighting the fact that response to poly(I:C) induction in fish seems to be complex and diverse. Moreover, four corresponding genes of the differentially expressed proteins were validated by relative quantitative real-time PCR. Western blot analysis further demonstrated the changes in protein abundance of natural killer enhancing factor and peroxiredoxin 6. These results will be helpful in furthering our understanding of the changes of physiological processes in liver of fish during virus infection. © 2013 Springer Science+Business Media Dordrecht. Source


Mu Y.,State Oceanic Administration | Lin K.,Fujian Fisheries Research Institute | Chen X.,State Oceanic Administration | Ao J.,State Oceanic Administration
Acta Oceanologica Sinica | Year: 2013

Viral nervous necrosis (VNN) causes high mortality in marine fish, especially in the grouper, worldwide and in China. Since there is no effective vaccine or drug to deal with VNN, early detection and prevention is important to block its outbreak. In this study, a reverse transcription-polymerase chain reaction (RT-PCR) was developed for the rapid, convenient, and sensitive detection of the VNN pathogen, nervous necrosis virus (NNV), in the grouper. The whole process was completed within 3.5 h from the RNA extraction to PCR product visualization. The detection limit of this method was 200 copies of NNV RNA standard, which corresponded to 200 copies of virus particles. This RT-PCR method was specific to the NNV detection with no cross-reactivity to other fish viral disease pathogens, such as infectious pancreatic necrosis virus (IPNV), infectious hematopoietic necrosis virus (IHNV), spring viraemia of carp virus (SVCV), epizootic haematopoietic necrosis virus (EHNV), and large yellow croaker iridovirus (LYCIV). With this method, the orange-spotted grouper (Epinephelus coioides) fry from hatcheries with or without incidence of the VNN epidemic in Fujian Province were detected. The results showed that all or 93% of the fry from the two hatcheries with incidence of the epidemic were diagnosed as positive, while 40% or 25% of fry from the two hatcheries without the VNN epidemic were also detected as NNV positive, indicating that this RT-PCR method can be used for rapid, sensitive detection of NNV infection and applied in the VNN epidemic alert. © 2013 The Chinese Society of Oceanography and Springer-Verlag Berlin Heidelberg. Source


He Q.,Wenzhou Medical College | Lu G.,Wenzhou Medical College | Che K.,Wenzhou Medical College | Zhao E.,Wenzhou Medical College | And 5 more authors.
Aquaculture | Year: 2011

The red spotted grouper, Epinephelus akaara, is an economically important aquaculture species in Southeast Asia. It is also classified as endangered in the Red List of Threatened Species. It is a protogynous hermaphrodite marine species, and the asynchronization of gamete production from both sexes has restricted mass seed production in artificial propagation. Cryopreservation can resolve these problems by storing sperm when they are abundant and to be used later for fertilization without any time constraint, yet sperm cryopreservation has not been explored in red spotted groupers. This study was intended to develop an optimal sperm cryopreservation protocol for this species. Specifically, we compared various activation solutions and found that maximum activation in red spotted grouper sperm can be achieved with glucose at 1100. mOsm/kg. Previous protocols used for the dusky grouper with 10% dimethyl sulfoxide (DMSO) plus 10. mg/ml bovine serum albumin (BSA) also yielded high post-thaw motility (70 ± 4%) for red spotted grouper in this study. Further optimization of cooling rate suggested a more rapid cooling of 61 °C/min or higher to be optimal for red spotted grouper. Addition of cholesterol in combination with 10% DMSO not only yielded high post-thaw motility (77 ± 5%) comparable to fresh sperm (87 ± 1%), but also prolonged motility duration to twice that of cryopreserved with the dusky grouper protocol (21 vs. 9. min). Sperm lipid measurement of fresh and thawed samples revealed a high retention of sphingomyelin (SM) content in samples that had high post-thaw motility. Future studies are necessary to further explore the role of SM in sperm cryopreservation. © 2011 Elsevier B.V. Source


Dai T.,Wenzhou Medical College | Zhao E.,Wenzhou Medical College | Lu G.,Wenzhou Medical College | Che K.,Wenzhou Medical College | And 8 more authors.
Aquaculture | Year: 2012

This study was intended to develop an optimal sperm cryopreservation protocol for yellow drum (Nibea albiflora), a commercially important fish species in East Asia. Specifically, we evaluated cryoprotectant type, concentration, and cooling rate and found that the highest fertilization (56-61%) and hatch (42-47%) can be obtained from yellow drum sperm suspended in 5% ethylene glycol or 10% dimethyl sulfoxide, cooled at 220°C/min, thawed in 40°C water bath for 7s, and fertilized with fresh (non-frozen) eggs at a sperm-to-egg ratio of 10 7. We also compared other sperm quality parameters such as sperm motility, motility duration, membrane integrity, mitochondrial membrane potential, and DNA fragmentation between fresh and thawed sperm samples. Although cryopreservation resulted in a significant increase in DNA fragmentation and significant decreases in the remaining parameters, the magnitude of these changes was small when compared to the 2000 to 3000-fold reduction in fertilization/hatch capability of thawed sperm. These findings suggest possible disruption of signaling pathways that cannot be detected by conventional quality assays. Further analysis of sperm membrane lipid composition revealed a significant negative correlation between sperm quality and cholesterol-to-sphingomyelin ratio. These findings indicate that lipid rafts could be the main targets for cryodamage-induced decrease in fertilization capability of thawed sperm. Future studies are necessary to further explore the role of lipid rafts in cryopreservation-mediated damage in fish sperm. © 2012 Elsevier B.V. Source


Qiu T.,CAS Qingdao Institute of Oceanology | Liu Y.,CAS Qingdao Institute of Oceanology | Zheng J.,CAS Qingdao Institute of Oceanology | Zhang T.,CAS Qingdao Institute of Oceanology | Qi J.,Fujian Fisheries Research Institute
Physiology and Behavior | Year: 2015

There is a need to develop more efficient rearing systems for the aquaculture of economically important bivalves, such as oysters. Here, we constructed a model that describes the feeding behavior of larval Crassostrea angulata oysters and tested it in an experimental setting. Larval ingestion rate is closely correlated with larval length. Based on our model, we showed that larval swimming speed, velum diameter and the filtration coefficient, which also determine the ingestion rate, are also correlated with larval length. Our model integrates morphological, locomotory and feeding behavior parameters to establish a relation between them and so provides a mathematical way to describe variation in the feeding behavior of bivalve larvae. The results of this study could facilitate the precise management of the aquaculture of bivalve larvae, in particular the optimum prey density and feeding rate of these important organisms. © 2015 Published by Elsevier Inc. Source

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