Zhao Y.,Fuzhou University |
Zheng J.,Fuzhou University |
Fang L.,Fuzhou University |
Lin Q.,Fujian Inspection and Research Institute for Product Quality |
And 3 more authors.
Talanta | Year: 2012
A environment-friendly microwave-assisted extraction used to extract trace mercury compounds from fish samples, and a ultra-sensitive method for the analysis of Hg(II), methylmercury (MeHg) and ethylmercury (EtHg) by using capillary electrophoresis-inductively coupled plasma mass spectrometry (CE-ICP-MS) were described in this study. The extraction method is environment-friendly, simple, effective, and can be used to extract trace mercury compounds in fish samples with a satisfied recovery within several minutes. The CE-ICP-MS analytical method has a detection limit as lower as 0.021-0.032 ng Hg/mL for MeHg, EtHg and Hg(II), and can be used to determined ultratrace MeHg, EtHg and Hg(II) in natural water and fish samples directly without any preconcentration. With the help of the above methods, we have successfully determined MeHg, EtHg and Hg(II) in dried fish (Tapertail anchovy) muscle and natural water within 25 min with a RSD (relative standard deviation, n = 6) <5% and a recovery of 94-103%. Our results showed that dried muscle of T. anchovy contained only one species of mercury, MeHg, indicating that MeHg is easier to be accumulated by aquatic organisms. © 2011 Elsevier B.V. All rights reserved.
Lu S.-Y.,Jilin University |
Lin C.,Jilin University |
Li Y.-S.,Jilin University |
Zhou Y.,Jilin University |
And 6 more authors.
Analytical Biochemistry | Year: 2012
A lateral flow immunochromatographic (LFIC) test strip based on a colloidal gold-monoclonal antibody (McAb) conjugate was developed for on-site rapid detection of okadaic acid (OA) in shellfish. It applies a competitive format using an immobilized toxin conjugate and free toxin present in samples. The McAb against OA was conjugated with 20-nm colloidal gold as detector reagent. The toxin in the sample competed with the immobilized toxin to bind to the gold conjugated with McAb. The colloidal gold/McAb/toxin mobile complex was not captured by OA-bovine serum albumin (BSA) on the test line, but it was captured by goat anti-mouse immunoglobulin G (IgG) on the control line. The color density of the test line correlated with the concentration of toxin in the range of 10-50 ng ml -1. The qualitative detection limit of 150 μg kg -1 sample was close to the European Union (EU) regulatory limit (160 μg kg -1). Therefore, these strips were able to directly and qualitatively estimate the consuming safety of shellfish. They require no equipment because of available visual results, and they screened numerous samples within 10 min. The results were further confirmed by high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). As a food safety screening tool, the test strips are convenient and useful to rapidly on-site test the presence of OA in shellfish products. © 2012 Elsevier Inc. All rights reserved.
Yang F.,Fuzhou University |
Xia S.,Fuzhou University |
Liu Z.,Fujian Entry exit Inspection and Quarantine Bureau |
Chen J.,Fujian Entry exit Inspection and Quarantine Bureau |
And 3 more authors.
Electrophoresis | Year: 2011
A method for the determination of methylene blue (MB) and its metabolites (azure A, azure B and azure C) in rat blood by CE-electrospray ionization mass spectrometry (CE-ESI-MS) was developed in this paper. Different analytical parameters were investigated in detail such as pH and concentration of separation buffer, and ESI-MS instrumental parameters. Under the optimum conditions, MB and its metabolites were separated and detected in 27.3min. LODs (defined as S/N=3) of this method were 0.22, 0.25, 0.10 and 0.30μg/mL for MB, azure A, azure B and azure C, respectively. To get a satisfactory extraction efficiency of MB and its metabolites in rat blood, different extraction solutions were studied. By using this method, MB and its metabolites (azure A, azure B and azure C) were successfully analyzed in rat blood samples. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Chen Y.,Fuzhou University |
Huang L.,Fuzhou University |
Wu W.,Fuzhou University |
Ruan Y.,Fuzhou University |
And 3 more authors.
Electrophoresis | Year: 2014
We herein reported a environment-friendly microwave-assisted extraction used to extract trace lead compounds from marine animals and a ultrasensitive method for the analysis of Pb2+, trimethyl lead chloride (TML) and triethyl lead chloride (TEL) by using CE-ICP-MS. The extraction method is simple and has a high extracting efficiency. It can be used to completely extract both inorganic lead and organolead in marine animal samples without altering its species. The analytical method has a detection limit as low as 0.012-0.084 ng Pb/mL for Pb2+, TML, and TEL, and can be used to determine ultratrace Pb2+, TML, and TEL in marine animals directly without any preconcentration. With the help of above methods, we have successfully determined Pb2+, TML, and TEL in clam and oyster tissue within 20 min with a RSD (n = 6) < 5% and a recovery of 91-104%. Our results showed that Pb2+ was the main species of lead in clam and oyster, and organolead (TML) was only found in oyster. The proposed method provides a realistic approach for the accurate evaluation of lead pollution in seafood. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Yang F.,Fuzhou University |
Liu Z.,Fujian Entry Exit Inspection and Quarantine Bureau |
Lin Y.,Fujian Entry Exit Inspection and Quarantine Bureau |
Chen J.,Fujian Entry Exit Inspection and Quarantine Bureau |
And 2 more authors.
Food Analytical Methods | Year: 2012
β-Agonists have been abused as the growth-promoting agent in food-producing animals over 20 years. The proof of using illegal drugs in food, which is necessary for a regulatory action, usually requires a high degree of specificity and sensitivity. This paper reported an ultra-high performance liquid chromatography (UHPLC)-ESI/MS/MS method for the confirmation of multi-residues of the 13 β-agonist compounds in milk. A wide range of analytes related to β-agonists (brombuterol, cimaterol, clenbuterol, clenpenterol, clorprenaline, hydroxymethylclenbuterol, isoxsuprine, mabuterol, ractopamine, ritodrine, salbutamol, terbutaline, tulobuterol) with similar chemical structures was investigated in order to demonstrate the applicability of our method. This method consists of a two-step extraction and a MCX SPE cleanup. The final extract was separated by UHPLC within 5 min and then injected in an electrospray ionization mass spectrometry for the determination. Using clenbuterol-D9, salbutamol-D3, and ractopamine-D5 as internal standards, and accomplishing with the matrix matched calibration curves to compensate for the matrix effects, the quantitative data showed good linear response within the concentration ranges studied. The detection limits (CCα) and detection capabilities (CCβ) of the analytes were found in the range of 0.01-0.16 μg/L and 0.03-0.21 μg/L, respectively. Recoveries of the compounds were found from 82.5% to 101% at the spiked level of 0.05-2.5 μg/L, and the relative standard deviation was within the range of 7.17% and 16.4%. Furthermore, an inter-laboratory study among eight laboratories was conducted to further validate the method, and the results were found satisfactory. © 2011 Springer Science+Business Media, LLC.
Zhou Z.-S.,Chinese Academy of Agricultural Sciences |
Rasmann S.,University of California at Irvine |
Li M.,Fujian Entry Exit Inspection and Quarantine Bureau |
Guo J.-Y.,Chinese Academy of Agricultural Sciences |
And 2 more authors.
PLoS ONE | Year: 2013
The leaf beetle, Ophraella communa, has been introduced to control the spread of the common ragweed, Ambrosia artemisiifolia, in China. We hypothesized that the beetle, to be able to track host-range expansion into colder climates, can phenotypically adapt to cold temperatures across generations. Therefore, we questioned whether parental experience of colder temperatures increases cold tolerance of the progeny. Specifically, we studied the demography, including development, fecundity, and survival, as well as physiological traits, including supercooling point (SCP), water content, and glycerol content of O. communa progeny whose parents were maintained at different temperature regimes. Overall, the entire immature stage decreased survival of about 0.2%-4.2% when parents experienced cold temperatures compared to control individuals obtained from parents raised at room temperature. However, intrinsic capacity for increase (r), net reproductive rate (R0) and finite rate of increase (λ) of progeny O. communa were maximum when parents experienced cold temperatures. Glycerol contents of both female and male in progeny was significantly higher when maternal and paternal adults were cold acclimated as compared to other treatments. This resulted in the supercooling point of the progeny adults being significantly lower compared to beetles emerging from parents that experienced room temperatures. These results suggest that cold hardiness of O. communa can be promoted by cold acclimation in previous generation, and it might counter-balance reduced survival in the next generation, especially when insects are tracking their host-plants into colder climates. © 2013 Zhou et al.
Xu D.,Xiamen Entry Exit Inspection and Quarantine Bureau |
Lu S.,Fujian Entry Exit Inspection and Quarantine Bureau |
Chen D.,Xiamen Entry Exit Inspection and Quarantine Bureau |
Lan J.,Fujian Entry Exit Inspection and Quarantine Bureau |
And 3 more authors.
Chinese Journal of Chromatography (Se Pu) | Year: 2013
An effective method was developed and applied to determine the residues often pesticides of pyrazoles and pyrroles in tea by accelerated solvent extraction coupled with gas chromatography-tandem mass spectrometry (ASE-GC-MS/MS).-The samples were extracted with ethyl acetate-hexane(l: 1, v/v) for 5 min at 1. 03 × 107 pa and 100 oC for one cycle. Then, they were purified by Envi-Carb/PSA column, and eluted by ethyl acetate-hexane (1: 1, v/v). The analytes were determined by GC-MS/MS and quantified by external standard method. The limits of quantification were 0.003 mg/kg for fenpyroximate, 0.001 mg/kg for fipronil-sulfide, 0.002 mg/kg for fipronil, 0.005 mg/kg for fipronil-sulfone, 0.002 mg/kg for chlorfenapyr, 0.006 mg/kg for flusilazole, 0.001 mg/kg for difenzoquat, 0.001 mg/kg for pyraflufen-ethyl, 0. 000 3 mg/kg for tebufenpyrad and 0. 005 mg/kg for tolfenpyrad. The results show that the proposed method is sensitive and accurate for the determination of the ten pesticide residues.
Wei-chuan Z.,Fujian Entry Exit Inspection and Quarantine Bureau |
Gui-ming Z.,Shenzhen Entry Exit Inspection and Quarantine Bureau
Journal of Plant Pathology | Year: 2010
Tilletia indica Mitra, the cause of Karnal bunt of wheat, is an important quarantine disease around the world. The Humid Thermal Index (HTI) model, and the bioclimate zoning map of wheat in China are based on critical values of key climatic factors during the susceptible period (heading and anthesis of wheat). We have calculated improvements to the HTI value for both early and late sown wheat varieties, with 21 days as a cycle (heading and anthesis period) and 1 day as a slip statistical unit. A GeoPhytopathology Model (GPMTI) for T. indica was built using a Microsoft Access Database, Map- Info MapX and Visual Basic, and was used to predict the potential geographic distribution areas of T. indica using climate data collected in the past 30 years from 670 meteorological stations. The results showed that the pathogen could establish itself in 60% of sites in China (nearly 4,580,000 km2), and this region can be divided into four areas: safe areas (HTI value for all periods unsuitable for disease development), low risk areas (HTI value for less than one-third of the periods suitable for disease development), risk areas (HTI value for between one-third and two-thirds of the periods suitable for disease development) and high risk areas (HTI value for more than two-thirds of the periods suitable for disease development). The risk areas and high risk areas were about 3,210,000 km2.
Qiu Y.,Fujian Entry Exit Inspection and Quarantine Bureau
Se pu = Chinese journal of chromatography / Zhongguo hua xue hui | Year: 2012
A liquid chromatography-tandem mass spectrometry method was established for the determination of virginiamycin M1 and S1 residues in livestock and poultry products. The sample was extracted by methanol-acetonitrile solution (1:1, v/v). The supernatant was diluted with 0.01 mol/L ammonium dihydrogen phosphate solution, then purified and concentrated on an Oasis HLB cartridge. The separation of virginiamycin M1 and S1 was performed on a Luna C18 column with the mobile phases acetonitrile and 5 mmol/L ammonium acetate aqueous solution (containing 0.1% (v/v) formic acid) in a gradient elution mode. The identification and quantification of the drugs were carried out by positive electrospray ionization (ESI + ) in the multiple reaction monitoring (MRM) mode using external standard method. The calibration curves showed good linearity in the range of 0.15-10.0 microg/L with correlation coefficients (r2) above 0. 999. The limits of quantities (LOQs) were both 0.25 microg/kg. The average recoveries of the two drugs spiked at 0.25, 0.5 and 2.5 microg/kg levels in different matrices were between 71.2% and 98.4%, and the relative standard deviations (RSDs) were between 3.6% and 15.4%. The method is simple, rapid, sensitive and accurate. It is suitable for the confirmation and quantification of virginiamycin M1 and S1 residues in livestock and poultry products.
PubMed | Fujian Entry Exit Inspection and Quarantine Bureau and Chinese Academy of Inspection and Quarantine
Type: Journal Article | Journal: Genome announcements | Year: 2015
Suncus murinus has been identified as the host for Seoul virus (SEOV). Here, we report the complete genome sequence of SEOV strain Fj372/2013, which was isolated from the lung tissue of Suncus murinus in the Fujian Province of China. A mutation A38C was observed in an open reading fragment of the middle segment.