Lin J.,Wuhan University |
Lin J.,Fujian Commercial College |
Du J.,Wuhan University
Acta Mathematica Scientia | Year: 2014
In this article, by using the stability of Cauchy type integral when the smooth perturbation for integral curve and the Sobolev type perturbation for kernel density happen, we discuss the stability of the second fundamental problem in plane elasticity when the smooth perturbation for the boundary of the elastic domain (unit disk) and the Sobolev type perturbation for the displacement happen. And the error estimate of the displacement between the second fundamental problem and its perturbed problem is obtained. © 2014 Wuhan Institute of Physics and Mathematics.
Zhu H.-Y.,Fujian Agriculture and forestry University |
Fu X.-P.,Zhejiang University |
You G.-R.,Fujian Commercial College |
He J.-C.,Fujian Agriculture and forestry University
Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis | Year: 2015
By extracting the Near Infrared (NIR) diffuse reflectance spectral characteristics from the post-harvest lotus seeds in different storage periods, the quantitative and qualitative analysis were applied to lotus seeds with the Soluble Solids Content (SSC) and dry matter content (DM) as criteria. The results of the Partial Least Squares Regression (PLSR) and distance discrimination (DA) models showed that the absorption spectra of lotus seeds and lotus kernels has clear relations to their SSC and DM. The PLSR models of SSC and DM of lotus seeds had the best performance in 5 941~12 480 cm-1 spectral region in this study. Their correlation coefficients of prediction were 0.74 and 0.82, and the correlation coefficients of calibration were 0.82 and 0.84, and the correlation coefficients of leave one out cross validation were 0.72 and 0.71. The PLSR model of SSC of lotus kernels was better in 7 891~9 310 cm-1 spectral region. Its correlation coefficient of prediction was 0.79, and the correlation coefficient of calibration was 0.84, and the correlation coefficient of leave one out cross validation was 0.77. The PLSR model of DM of lotus kernels is better in the full spectral region. Its correlation coefficient of prediction was 0.92, and the correlation coefficient of calibration was 0.89, and the correlation coefficient of leave one out cross validation was 0.82. For lotus seeds, the DA model in 5 400~7 885 cm-1 spectral region is the best with a correctness of 84.2%. And for lotus kernels, the DA model in 9 226~12 480 cm-1 spectral region is the best with a correctness of 90.8%. For dry lotus kernels, the discriminant accuracy of the DA model is 98.9% in the optimal spectral region. All kernels with membrane and plumule were correctly discriminated. This research shows that the NIR spectroscopy technique can be used to determine SSC and DM content of lotus seeds and lotus kernels, as well as to discriminate their freshness and also to discriminate dry lotus kernels of different age and the kernels with membrane and plumule. © 2015, Science Press. All right reserved.
Ling S.,Fujian Agriculture and forestry University |
Chen Q.-A.,Fujian Commercial College |
Chen Q.-A.,Fujian Agriculture and forestry University |
Zhang Y.,Fujian Agriculture and forestry University |
And 4 more authors.
Biosensors and Bioelectronics | Year: 2015
A monoclonal hybridoma cell named 5B9 against tetrodotoxin (TTX) was obtained after fusion of myeloma SP2/0 cells with spleen cells isolated from the immunized Balb/c mice. The 5B9 monoclonal antibody (McAb) with high affinity (about 2.55×109) is specific to TTX, and this McAb belongs to the immunoglobulin G (IgG) isotype. Finally, an enzyme-linked immunosorbent assay (ELISA) and colloidal gold immunoassay were established based on this McAb. The linear range of ELISA to detect TTX was 5-500ng/mL, and the limit of detection (LOD) was 4.44ng/mL. The average CV of intra- and inter-assay was less than 8%, with the samples recovery range of 70.93-99.99%. A competitive format colloidal gold strip was developed for detection of TTX in real samples, and the LOD for TTX is 20ng/mL, and the assay time of the qualitative test can be finished in less than 10min without any equipment. The result from test strip revealed that the test strip has a good agreement with those obtained from ELISA. © 2015.
Juan L.,Fujian Commercial College |
Chuanrong W.,Fuzhou University |
Kunmei Y.,Fujian Commercial College
Mathematical Methods in the Applied Sciences | Year: 2010
Let the elastic domain be a disk, and its boundary the unit circle. By dint of the stability of Cauchy-type integral with respect to the perturbation of integral curve, this paper discusses the stability of the complex stress functions to the second fundamental problem in plane elasticity when smooth perturbation happens for the boundary curve. Copyright © 2010 John Wiley & Sons, Ltd.