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Zheng B.,Xiamen University | Bao C.,Xiamen University | Huang H.,Xiamen University | Ye H.,Xiamen University | Ye H.,Fujian Collab Innovation Center For Exploitation And Utilization Of Marine Biological Resources
Reproduction | Year: 2016

As the precursor of vitellin (Vn), vitellogenin (Vg) has initially been considered as a female-specific protein involved in vitellogenesis, while it was also present in males induced by hormones or organs manipulation. Distinct from vtg1 we previously found in female mud crab Scylla paramamosain, vtg2 was intriguingly detected in male testis under normal physiological conditions in this study. Sequence analysis showed that vtg2 and vtg1 were actually two isoforms of Vg caused by different types of alternative splicing. PCR and in situ hybridization analysis revealed that vtg2 was localized only in the testicular spermatozoa, while Vn was detected in both the spermatozoa of the testis and seminal vesicle. Therefore, we speculated that Vn was initially translated in testicular spermatozoa, then migrated with spermatozoa, and finally stored in the seminal vesicle, where spermatozoa gradually accomplished maturation. We presumed that vtg2/Vn might act as an immune-relevant molecule in the male reproduction system. In the subsequent experiment, the expression of vtg2/Vn in testis was significantly induced in response to lipopolysaccharide (LPS) and lipoteichoic acid (LTA) injection at both transcriptional and translational levels. In the light of the results presented above, we deemed that vtg2/Vn is a novel candidate of immune-relevant molecules involved in immunoprotection during the spermatozoon maturation, and this research helps to open a new avenue for further exploring the role of Vg. © 2016 Society for Reproduction and Fertility.

Chen N.,Xiamen University | Chen N.,Fujian Collab Innovation Center For Exploitation And Utilization Of Marine Biological Resources | Luo X.,Xiamen University | Luo X.,Fujian Collab Innovation Center For Exploitation And Utilization Of Marine Biological Resources | And 8 more authors.
Journal of Shellfish Research | Year: 2016

Pacific abalone Haliotis discus hannai is one of the most important species of shellfish aquaculture. The annual production of cultured abalone exceeded 100,000 t in 2013, and the Pacific abalone was the main farmed species. The genetic diversity of hatchery stocks of H. discus hannai in most areas of China, however, remains unknown. The exchange of Pacific abalone in China was not only among hatcheries but also between southern and northern areas, and the Japanese population was widely used for the production of seed. Both practices may have led to the production of mixed seeds, and the difference between populations may be not significant, which could result in inbreeding and loss of potentially valuable alleles. Therefore, it is necessary to assess the current genetic characterization of hatchery populations. For the analysis, a total of 10 populations were obtained from different hatcheries: Dalian, Qingdao, and Changdao in the northern coastal areas; and Xiapu, Lianjiang, Putian, Zhangpu, Jinjiang, Dongshan, and Shanwei in the southern coastal areas. Five populations were added as the control: Ja (the F1 population of H. discus hannai introduced from Japan), KDH (the F1 population of H. discus hannai introduced from Korea), KDD (the F1 population of Haliotis discus discus introduced from Korea), ST (the F1 population developed from the cross of H. discus discus and H. discus hannai), and Red (the Pacific abalone with red shell). The data of 14 microsatellite primers were all highly polymorphic while the degree of variability was different at each locus. In all the studied populations, the number of alleles ranged from 2 to 17, and the number of effective alleles ranged from 1.032 to 10.169. The principal component analysis result showed that all populations were dispersed except KDD and ST, which clustered in the second and third quadrants. A neighborjoining tree indicated that most populations were closely related. Moreover KDH, KDD, and ST were less related to the others because of the different genetic background. The estimates of effective population size (Ne) using the linkage disequilibrium methods demonstrated that the Ne of eight populations was less than 50, and the other two populations were close to 50. In conclusion, by the previous studies, the current analyses present that these cultured populations of Pacific abalone in China have lost their genetic diversity to some extent, and the difference among populations was not significant. Thus, for the sustainable development of the aquaculture, the genetic diversity should be monitored every year and inbreeding should be controlled.

Han Y.,Xiamen University | Lei S.-L.,Xiamen University | Lu J.-H.,Xiamen University | He Y.,Xiamen University | And 5 more authors.
Materials Science and Engineering C | Year: 2016

A surface-enhanced Raman scattering (SERS)-assisted theranostic strategy was designed based on a synthesized multifunctional Fe3O4/Au cluster/shell nanocomposite. This theranostic strategy was used for free prostate specific antigen (free-PSA) detection, magnetic resonance imaging (MRI), and magnetic hyperthermia. The lowest protein concentration detected was 1 ng mL- 1, and the limit of detection (LOD) of the calculated PSA was 0.75 ng mL- 1. Then, MRI was carried out to visualize the tumor cell. Lastly, magnetic hyperthermia was employed and revealed a favorable killing effect for the tumor cells. Thus, this SERS-assisted strategy based on a Fe3O4/Au cluster/shell nanocomposite showed great advantages in theranostic treatment. © 2016 Elsevier B.V. All rights reserved.

Yuan J.,State Oceanic Administration | Yuan J.,Xiamen University | Yuan J.,Fujian Collab Innovation Center For Exploitation And Utilization Of Marine Biological Resources | Lai Q.,State Oceanic Administration | And 7 more authors.
Frontiers in Microbiology | Year: 2015

The bacteria involved in organic pollutant degradation in pelagic deep-sea environments are largely unknown. In this report, the diversity of polycyclic aromatic hydrocarbon (PAH)-degrading bacteria was analyzed in deep-sea water on the Southwest Indian Ridge (SWIR). After enrichment with a PAH mixture (phenanthrene, anthracene, fluoranthene, and pyrene), nine bacterial consortia were obtained from depths of 3946-4746 m. While the consortia degraded all four PAHs when supplied in a mixture, when PAHs were tested individually, only phenanthrene supported growth. Thus, degradation of the PAH mixture reflected a cometabolism of anthracene, fluoranthene, and pyrene with phenanthrene. Further, both culture-dependent and independent methods revealed many new bacteria involved in PAH degradation. Specifically, the alpha and gamma subclasses of Proteobacteria were confirmed as the major groups within the communities. Additionally, Actinobacteria, the CFB group and Firmicutes were detected. Denaturing Gradient Gel Electrophoresis (DGGE) analysis showed that bacteria closely affiliated with Alcanivorax, Novosphingobium, and Rhodovulum occurred most frequently in different PAH-degrading consortia. By using general heterotrophic media, 51 bacteria were isolated from the consortia and of these 34 grew with the PAH mixture as a sole carbon source. Of these, isolates most closely related to Alterierythrobacter, Citricella, Erythrobacter, Idiomarina, Lutibacterium, Maricaulis, Marinobacter, Martelella, Pseudidiomarina, Rhodobacter, Roseovarius, Salipiger, Sphingopyxis, and Stappia were found to be PAH degraders. To the best of our knowledge, this is the first time these bacteria have been identified in this context. In summary, this report revealed significant diversity among the PAH-degrading bacteria in the deep-sea water column. These bacteria may play a role in PAH removal in deep-sea environments. © 2015 Yuan, Lai, Sun, Zheng and Shao.

Lin Z.-Z.,Jimei University | Lin Z.-Z.,Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering | Zhang H.-Y.,Jimei University | Peng A.-H.,Jimei University | And 5 more authors.
Food Chemistry | Year: 2016

Magnetic molecularly imprinted polymers (MMIPs) were synthesized through precipitation polymerization using malachite green (MG) as template, methacrylic acid as monomer, ethylene dimethacrylate as crosslinker, and Fe3O4 magnetite as magnetic component. MMIPs were characterized by scanning electron microscopy, Fourier transform infrared spectrometry, and vibrating sample magnetometry. Under the optimum condition, the MMIPs obtained exhibited quick binding kinetics and high affinity to MG in the solution. Scatchard plot analysis revealed that the MMIPs contained only one type of binding site with dissociation constant of 24.0 μg mL-1. The selectivity experiment confirmed that the MMIPs exhibited higher selective binding capacity for MG than its structurally related compound (e.g., crystal violet). As a sorbent for the extraction of MG in sample preparation, MMIPs together with the absorbed analytes could easily be separated from the sample matrix with an external magnet. After elution with methanol/acetic acid (9:1, v/v), MG in the eluent was determined by high-performance liquid chromatography coupled with UV detector with recoveries of 94.0-115%. Results indicated that the as-prepared MMIPs are promising materials for MG analysis in aquatic products. © 2016 Elsevier Ltd. All rights reserved.

Gao Z.-H.,Jimei University | Lin Z.-Z.,Jimei University | Lin Z.-Z.,Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering | Chen X.-M.,Jimei University | And 3 more authors.
Analytical Methods | Year: 2016

A facile and green hydrothermal method was developed for the preparation of highly luminescent nitrogen-doped carbon dots (NCDs) by using anhydrous citric acid and urea as the carbon source and nitrogen source, respectively. The NCDs show good water-solubility and exhibit excitation-independent fluorescence behaviors at excitations of 300-390 nm with a quantum yield of 42.5% at a λem of 440 nm. Based on the fluorescence quenching strategy, the NCDs were successfully applied to the measurement of Hg2+ in tap and lake water samples with high sensitivity and excellent selectivity. The detection limit was 7.3 nmol L-1 (3σ, n = 9), indicating its potential applications for the detection of trace Hg2+ in water samples. © The Royal Society of Chemistry 2016.

Jiang T.-L.,Jimei University | Cai Q.-F.,Jimei University | Cai Q.-F.,Fujian Collab Innovation Center For Exploitation And Utilization Of Marine Biological Resources | Shen J.-D.,Jimei University | And 7 more authors.
LWT - Food Science and Technology | Year: 2015

Recently, adulteration by adding soybean proteins to surimi products to replace fish muscle has been gaining momentum. In this study, soybean trypsin inhibitor (STI) was chosen as target protein for detection of soybean proteins in surimi products and both polyclonal antibody and monoclonal antibody (A11-6) against STI were prepared. A semi-quantitative means of analyzing soybean proteins in various commercial surimi products was performed by Western blot. A sandwich enzyme-linked immunosorbent assay (s-ELISA) was further developed to quantitatively detect soybean proteins in surimi products. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.68 μg SPI/mL (13.6 mg SPI/kg food) and 3.09 μg SPI/mL (61.7 mg SPI/kg food), respectively. The rates of recovery of soybean proteins ranged from 100.1% to 122.2%, and the coefficient of variation (CV) was less than 4.1%. Our results indicated that the methods established can be applied to monitor soybean proteins in surimi products. © 2015 Elsevier Ltd.

Luo Z.-H.,State Oceanic Administration | Luo Z.-H.,Fujian Collab Innovation Center For Exploitation And Utilization Of Marine Biological Resources | Yu Y.-P.,State Oceanic Administration | Yu Y.-P.,Fujian Collab Innovation Center For Exploitation And Utilization Of Marine Biological Resources | And 5 more authors.
Marine Genomics | Year: 2015

A Vibrio sp. lytic phage VH7D was isolated from seawater of an abalone farm in Xiamen, China. The phage was capable of lysing Vibrio rotiferianus DSM 17186T and Vibrio harveyi DSM 19623T. The complete genome of this phage consists of 246,964 nucleotides with a GC content of 41.31%, which characterized it as a giant vibriophage. Here we report the complete genome sequence and major findings from the genomic annotation. © 2015 Elsevier B.V.

Ao J.,State Oceanic Administration | Ao J.,Fujian Collab Innovation Center For Exploitation And Utilization Of Marine Biological Resources | Li Q.,State Oceanic Administration | Li Q.,Fujian Collab Innovation Center For Exploitation And Utilization Of Marine Biological Resources | And 4 more authors.
Fish and Shellfish Immunology | Year: 2016

Cystatin F, a member of the family II cystatins, plays important roles in immune response-related processes through inhibiting specific enzyme targets. In this study, a cystatin F homologue, LycCysF, was identified and characterized from large yellow croaker (Larimichthys crocea). The deduced LycCysF protein exhibits a typical structural feature of type II cystatins, including three evolutionally conserved motifs, Gly35, QVVRG79-83 and PW130-131. Tissue expression analysis showed that LycCysF mRNA was expressed in all tissues examined, albeit at different levels. Recombinant LycCysF (rLycCysF) produced in Pichia pastoris could inhibit the activity of multiple cysteine proteases, including papain, legumain and recombinant large yellow croaker cathepsin B, L and S. Moreover, rLycCysF could inhibit the Ii chain processing by recombinant cathepsin S in vitro. These data suggest that LycCysF may participate in regulation of cathepsins and MHC-II associated Ii chain processing. In addition, mammalian cystatin F is produced as an inactive dimer, becoming activated by proteolysis in the endo/lysosome of immune cells and then exerts its function of regulating downstream proteases activity. However, the N-terminal extension and two additional cysteine residues responsible for dimer formation are absent in LycCysF and cystatin F from other fish species, reptiles and Aves, indicating that these proteins can not form dimer and may regulate the proteases activity via an alternate pathway distinct from mammalian cystatin F. To our knowledge, this is the first report on molecular characteristics of a teleost cystatin F and its role in Ii chain processing. © 2015 Elsevier Ltd.

Wang W.,Third Institute of Oceanography | Wang W.,Fujian Collab Innovation Center For Exploitation And Utilization Of Marine Biological Resources | Shao Z.,Third Institute of Oceanography | Shao Z.,Fujian Collab Innovation Center For Exploitation And Utilization Of Marine Biological Resources
Nature Communications | Year: 2015

Alkane-degrading bacteria are ubiquitous in marine environments, but little is known about how alkane degradation is regulated. Here we investigate alkane sensing, chemotaxis, signal transduction, uptake and pathway regulation in Alcanivorax dieselolei. The outer membrane protein OmpS detects the presence of alkanes and triggers the expression of an alkane chemotaxis complex. The coupling protein CheW2 of the chemotaxis complex, which is induced only by long-chain (LC) alkanes, sends signals to trigger the expression of Cyo, which participates in modulating the expression of the negative regulator protein AlmR. This change in turn leads to the expression of ompT1 and almA, which drive the selective uptake and hydroxylation of LC alkanes, respectively. AlmA is confirmed as a hydroxylase of LC alkanes. Additional factors responsible for the metabolism of medium-chain-length alkanes are also identified, including CheW1, OmpT1 and OmpT2. These results provide new insights into alkane metabolism pathways from alkane sensing to degradation. © 2014 Macmillan Publishers Limited. All rights reserved.

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