Fujian Collab Innovation Center For Exploitation And Utilization Of Marine Biological Resources

of Marine, China

Fujian Collab Innovation Center For Exploitation And Utilization Of Marine Biological Resources

of Marine, China
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Zhong T.-H.,State Oceanic Administration | Zhong T.-H.,Fujian Collab Innovation Center For Exploitation And Utilization Of Marine Biological Resources | Zhang J.-W.,State Oceanic Administration | Zhang J.-W.,Fujian Collab Innovation Center For Exploitation And Utilization Of Marine Biological Resources | And 6 more authors.
Botanica Marina | Year: 2017

As an important feedstock for biodiesel production, microbial oil has attracted considerable interest in recent years. Here, we investigated the effect of medium composition and culture conditions on cell growth and lipid accumulation of an oleaginous fungus Fusarium sp. 3A00501 on synthetic nitrogen-limited media. The optimal nitrogen source, carbon source, and C/N molar ratio in the medium for lipid accumulation were peptone, glucose, and 76, respectively. Additionally, the optimal initial pH of the medium and culture temperature for lipid production of the fungus were 6.0 and 30°C, respectively. Under the optimized fermentation conditions, the cell biomass decreased, but the lipid content and yield increased (by about 45% and 27%, respectively) compared with the unoptimized conditions. The microbial oil produced by the fungus mainly contained palmitic, elaidic, oleic, linolenic, heneicosanoic, and eicosadienoic acids. The unsaturated fatty acids accounted for about 65% of the total fatty acids. This high percentage of unsaturated fatty acids in the oil was similar to the plant oils commonly used in biodiesel production. These results suggest that Fusarium sp. 3A00501 might be a promising strain to provide lipids for biodiesel production. © 2017 Walter de Gruyter GmbH, Berlin/Boston.


Zhou W.,Central South University | Zhou W.,State Oceanic Administration | Zhou W.,Fujian Collab Innovation Center For Exploitation And Utilization Of Marine Biological Resources | Guo W.,State Oceanic Administration | And 4 more authors.
Microbiological Research | Year: 2016

Due to its toxicity and volatility, phenol must be cleared from the environment. Sulfobacillus acidophilus TPY, which was isolated from a hydrothermal vent in the Pacific Ocean as a moderately thermoacidophilic Gram-positive bacterium, was capable of aerobically degrading phenol. This bacterium could tolerate up to 1300 mg/L phenol and degrade 100 mg/L phenol in 40 h completely at 45 °C and pH 1.8 with a maximal degradation rate of 2.32 mg/L/h at 38 h. Genome-wide search revealed that one gene (TPY_3176) and 14 genes clustered together in two regions with locus tags of TPY_0628-0634 and TPY_0640-0646 was proposed to be involved in phenol degradation via the meta-pathway with both the 4-oxalocrotonate branch and the hydrolytic branch. Real-time PCR analysis of S. acidophilus TPY under phenol cultivation condition confirmed the transcription of proposed genes involved in the phenol degradation meta-pathway. Degradation of 3-methylphenol and 2-methylphenol confirmed that the hydrolytic branch was utilised by S. acidophilus TPY. Phylogenetic analysis revealed that S. acidophilus TPY was closely related to sulphate-reducing bacteria and some Gram-positive phenol-degrading bacteria. This was the first report demonstrating the ability of S. acidophilus to degrade phenol and characterising the putative genes involved in phenol metabolism in S. acidophilus TPY. © 2016 Elsevier GmbH.


Zheng B.,Xiamen University | Bao C.,Xiamen University | Huang H.,Xiamen University | Ye H.,Xiamen University | Ye H.,Fujian Collab Innovation Center For Exploitation And Utilization Of Marine Biological Resources
Reproduction | Year: 2016

As the precursor of vitellin (Vn), vitellogenin (Vg) has initially been considered as a female-specific protein involved in vitellogenesis, while it was also present in males induced by hormones or organs manipulation. Distinct from vtg1 we previously found in female mud crab Scylla paramamosain, vtg2 was intriguingly detected in male testis under normal physiological conditions in this study. Sequence analysis showed that vtg2 and vtg1 were actually two isoforms of Vg caused by different types of alternative splicing. PCR and in situ hybridization analysis revealed that vtg2 was localized only in the testicular spermatozoa, while Vn was detected in both the spermatozoa of the testis and seminal vesicle. Therefore, we speculated that Vn was initially translated in testicular spermatozoa, then migrated with spermatozoa, and finally stored in the seminal vesicle, where spermatozoa gradually accomplished maturation. We presumed that vtg2/Vn might act as an immune-relevant molecule in the male reproduction system. In the subsequent experiment, the expression of vtg2/Vn in testis was significantly induced in response to lipopolysaccharide (LPS) and lipoteichoic acid (LTA) injection at both transcriptional and translational levels. In the light of the results presented above, we deemed that vtg2/Vn is a novel candidate of immune-relevant molecules involved in immunoprotection during the spermatozoon maturation, and this research helps to open a new avenue for further exploring the role of Vg. © 2016 Society for Reproduction and Fertility.


Han Y.,Xiamen University | Lei S.-L.,Xiamen University | Lu J.-H.,Xiamen University | He Y.,Xiamen University | And 5 more authors.
Materials Science and Engineering C | Year: 2016

A surface-enhanced Raman scattering (SERS)-assisted theranostic strategy was designed based on a synthesized multifunctional Fe3O4/Au cluster/shell nanocomposite. This theranostic strategy was used for free prostate specific antigen (free-PSA) detection, magnetic resonance imaging (MRI), and magnetic hyperthermia. The lowest protein concentration detected was 1 ng mL- 1, and the limit of detection (LOD) of the calculated PSA was 0.75 ng mL- 1. Then, MRI was carried out to visualize the tumor cell. Lastly, magnetic hyperthermia was employed and revealed a favorable killing effect for the tumor cells. Thus, this SERS-assisted strategy based on a Fe3O4/Au cluster/shell nanocomposite showed great advantages in theranostic treatment. © 2016 Elsevier B.V. All rights reserved.


Yuan J.,State Oceanic Administration | Yuan J.,Xiamen University | Yuan J.,Fujian Collab Innovation Center For Exploitation And Utilization Of Marine Biological Resources | Lai Q.,State Oceanic Administration | And 7 more authors.
Frontiers in Microbiology | Year: 2015

The bacteria involved in organic pollutant degradation in pelagic deep-sea environments are largely unknown. In this report, the diversity of polycyclic aromatic hydrocarbon (PAH)-degrading bacteria was analyzed in deep-sea water on the Southwest Indian Ridge (SWIR). After enrichment with a PAH mixture (phenanthrene, anthracene, fluoranthene, and pyrene), nine bacterial consortia were obtained from depths of 3946-4746 m. While the consortia degraded all four PAHs when supplied in a mixture, when PAHs were tested individually, only phenanthrene supported growth. Thus, degradation of the PAH mixture reflected a cometabolism of anthracene, fluoranthene, and pyrene with phenanthrene. Further, both culture-dependent and independent methods revealed many new bacteria involved in PAH degradation. Specifically, the alpha and gamma subclasses of Proteobacteria were confirmed as the major groups within the communities. Additionally, Actinobacteria, the CFB group and Firmicutes were detected. Denaturing Gradient Gel Electrophoresis (DGGE) analysis showed that bacteria closely affiliated with Alcanivorax, Novosphingobium, and Rhodovulum occurred most frequently in different PAH-degrading consortia. By using general heterotrophic media, 51 bacteria were isolated from the consortia and of these 34 grew with the PAH mixture as a sole carbon source. Of these, isolates most closely related to Alterierythrobacter, Citricella, Erythrobacter, Idiomarina, Lutibacterium, Maricaulis, Marinobacter, Martelella, Pseudidiomarina, Rhodobacter, Roseovarius, Salipiger, Sphingopyxis, and Stappia were found to be PAH degraders. To the best of our knowledge, this is the first time these bacteria have been identified in this context. In summary, this report revealed significant diversity among the PAH-degrading bacteria in the deep-sea water column. These bacteria may play a role in PAH removal in deep-sea environments. © 2015 Yuan, Lai, Sun, Zheng and Shao.


Lin Z.-Z.,Jimei University | Lin Z.-Z.,Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering | Zhang H.-Y.,Jimei University | Peng A.-H.,Jimei University | And 5 more authors.
Food Chemistry | Year: 2016

Magnetic molecularly imprinted polymers (MMIPs) were synthesized through precipitation polymerization using malachite green (MG) as template, methacrylic acid as monomer, ethylene dimethacrylate as crosslinker, and Fe3O4 magnetite as magnetic component. MMIPs were characterized by scanning electron microscopy, Fourier transform infrared spectrometry, and vibrating sample magnetometry. Under the optimum condition, the MMIPs obtained exhibited quick binding kinetics and high affinity to MG in the solution. Scatchard plot analysis revealed that the MMIPs contained only one type of binding site with dissociation constant of 24.0 μg mL-1. The selectivity experiment confirmed that the MMIPs exhibited higher selective binding capacity for MG than its structurally related compound (e.g., crystal violet). As a sorbent for the extraction of MG in sample preparation, MMIPs together with the absorbed analytes could easily be separated from the sample matrix with an external magnet. After elution with methanol/acetic acid (9:1, v/v), MG in the eluent was determined by high-performance liquid chromatography coupled with UV detector with recoveries of 94.0-115%. Results indicated that the as-prepared MMIPs are promising materials for MG analysis in aquatic products. © 2016 Elsevier Ltd. All rights reserved.


Gao Z.-H.,Jimei University | Lin Z.-Z.,Jimei University | Lin Z.-Z.,Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering | Chen X.-M.,Jimei University | And 3 more authors.
Analytical Methods | Year: 2016

A facile and green hydrothermal method was developed for the preparation of highly luminescent nitrogen-doped carbon dots (NCDs) by using anhydrous citric acid and urea as the carbon source and nitrogen source, respectively. The NCDs show good water-solubility and exhibit excitation-independent fluorescence behaviors at excitations of 300-390 nm with a quantum yield of 42.5% at a λem of 440 nm. Based on the fluorescence quenching strategy, the NCDs were successfully applied to the measurement of Hg2+ in tap and lake water samples with high sensitivity and excellent selectivity. The detection limit was 7.3 nmol L-1 (3σ, n = 9), indicating its potential applications for the detection of trace Hg2+ in water samples. © The Royal Society of Chemistry 2016.


Jiang T.-L.,Jimei University | Cai Q.-F.,Jimei University | Cai Q.-F.,Fujian Collab Innovation Center For Exploitation And Utilization Of Marine Biological Resources | Shen J.-D.,Jimei University | And 7 more authors.
LWT - Food Science and Technology | Year: 2015

Recently, adulteration by adding soybean proteins to surimi products to replace fish muscle has been gaining momentum. In this study, soybean trypsin inhibitor (STI) was chosen as target protein for detection of soybean proteins in surimi products and both polyclonal antibody and monoclonal antibody (A11-6) against STI were prepared. A semi-quantitative means of analyzing soybean proteins in various commercial surimi products was performed by Western blot. A sandwich enzyme-linked immunosorbent assay (s-ELISA) was further developed to quantitatively detect soybean proteins in surimi products. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.68 μg SPI/mL (13.6 mg SPI/kg food) and 3.09 μg SPI/mL (61.7 mg SPI/kg food), respectively. The rates of recovery of soybean proteins ranged from 100.1% to 122.2%, and the coefficient of variation (CV) was less than 4.1%. Our results indicated that the methods established can be applied to monitor soybean proteins in surimi products. © 2015 Elsevier Ltd.


Ao J.,State Oceanic Administration | Ao J.,Fujian Collab Innovation Center For Exploitation And Utilization Of Marine Biological Resources | Li Q.,State Oceanic Administration | Li Q.,Fujian Collab Innovation Center For Exploitation And Utilization Of Marine Biological Resources | And 4 more authors.
Fish and Shellfish Immunology | Year: 2016

Cystatin F, a member of the family II cystatins, plays important roles in immune response-related processes through inhibiting specific enzyme targets. In this study, a cystatin F homologue, LycCysF, was identified and characterized from large yellow croaker (Larimichthys crocea). The deduced LycCysF protein exhibits a typical structural feature of type II cystatins, including three evolutionally conserved motifs, Gly35, QVVRG79-83 and PW130-131. Tissue expression analysis showed that LycCysF mRNA was expressed in all tissues examined, albeit at different levels. Recombinant LycCysF (rLycCysF) produced in Pichia pastoris could inhibit the activity of multiple cysteine proteases, including papain, legumain and recombinant large yellow croaker cathepsin B, L and S. Moreover, rLycCysF could inhibit the Ii chain processing by recombinant cathepsin S in vitro. These data suggest that LycCysF may participate in regulation of cathepsins and MHC-II associated Ii chain processing. In addition, mammalian cystatin F is produced as an inactive dimer, becoming activated by proteolysis in the endo/lysosome of immune cells and then exerts its function of regulating downstream proteases activity. However, the N-terminal extension and two additional cysteine residues responsible for dimer formation are absent in LycCysF and cystatin F from other fish species, reptiles and Aves, indicating that these proteins can not form dimer and may regulate the proteases activity via an alternate pathway distinct from mammalian cystatin F. To our knowledge, this is the first report on molecular characteristics of a teleost cystatin F and its role in Ii chain processing. © 2015 Elsevier Ltd.


Wang W.,Third Institute of Oceanography | Wang W.,Fujian Collab Innovation Center For Exploitation And Utilization Of Marine Biological Resources | Shao Z.,Third Institute of Oceanography | Shao Z.,Fujian Collab Innovation Center For Exploitation And Utilization Of Marine Biological Resources
Nature Communications | Year: 2015

Alkane-degrading bacteria are ubiquitous in marine environments, but little is known about how alkane degradation is regulated. Here we investigate alkane sensing, chemotaxis, signal transduction, uptake and pathway regulation in Alcanivorax dieselolei. The outer membrane protein OmpS detects the presence of alkanes and triggers the expression of an alkane chemotaxis complex. The coupling protein CheW2 of the chemotaxis complex, which is induced only by long-chain (LC) alkanes, sends signals to trigger the expression of Cyo, which participates in modulating the expression of the negative regulator protein AlmR. This change in turn leads to the expression of ompT1 and almA, which drive the selective uptake and hydroxylation of LC alkanes, respectively. AlmA is confirmed as a hydroxylase of LC alkanes. Additional factors responsible for the metabolism of medium-chain-length alkanes are also identified, including CheW1, OmpT1 and OmpT2. These results provide new insights into alkane metabolism pathways from alkane sensing to degradation. © 2014 Macmillan Publishers Limited. All rights reserved.

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