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Chen J.,Shanghai JiaoTong University | Wang W.,Sun Yat Sen University | Zhang T.,Fujian Academy of Medical Science | Ji J.,Shanghai JiaoTong University | And 5 more authors.
PLoS ONE | Year: 2012

Background: Chronic inflammation plays a causal role in gastric tumor initiation. The identification of predictive biomarkers from gastric inflammation to tumorigenesis will help us to distinguish gastric cancer from atrophic gastritis and establish the diagnosis of early-stage gastric cancer. Phospholipase C epsilon 1 (PLCε1) is reported to play a vital role in inflammation and tumorigenesis. This study was aimed to investigate the clinical significance of PLCε1 in the initiation and progression of gastric cancer. Methodology/Principal Findings: Firstly, the mRNA and protein expression of PLCε1 were analyzed by reverse transcription-PCR and Western blotting in normal gastric mucous epithelial cell line GES-1 and gastric cancer cell lines AGS, SGC7901, and MGC803. The results showed both mRNA and protein levels of PLCε1 were up-regulated in gastric cancer cells compared with normal gastric mucous epithelial cells. Secondly, this result was confirmed by immunohistochemical detection in a tissue microarray including 74 paired gastric cancer and adjacent normal tissues. Thirdly, an independence immunohistochemical analysis of 799 chronic atrophic gastritis tissue specimens demonstrated that PLCε1 expression in atrophic gastritis tissues were down-regulated since PLCε1 expression was negative in 524 (65.6%) atrophic gastritis. In addition, matched clinical tissues from atrophic severe gastritis and gastric cancer patients were used to further confirm the previous results by analyzing mRNA and protein levels expression of PLCε1 in clinical samples. Conclusions/Significances: Our results suggested that PLCε1 protein may be a potential biomarker to distinguish gastric cancer from inflammation lesion, and could have great potential in applications such as diagnosis and pre-warning of early-stage gastric cancer. © 2012 Chen et al.


PubMed | China Institute of Technology, Fujian Academy of Medical science and Fujian Agriculture and forestry University
Type: Journal Article | Journal: Journal of the science of food and agriculture | Year: 2016

The genus Monascus includes several species of fungi valued across Asia for their culinary uses and diverse medicinal properties. In this study, we evaluated the applicability of random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers in characterizing the genetic diversity in 41 Monascus strains collected from various regions of Fujian Province, the leading producer of Monascus in China.Seven screened ISSR primers generated 56 polymorphic bands, of which 93.33% were polymorphic. The genetic similarity coefficients (GSC) of the strains ranged from 0.50 to 1.00. Comparative sequence analysis using seven screened RAPD primers amplified a total of 49 polymorphic bands, of which 81.67% were polymorphic; GSC values ranged from 0.62 to 1.00.Correlation analysis revealed a significant positive correlation in genetic distances assessed using above two markers, which indicated they were suitable for Monascus species characterization. ISSR markers were more suitable for the classification and determination of Monascus species, while RAPD markers appear to be preferable for analyzing the differences among strains within the same species. Our study revealed that Monascus possesses rich genetic diversity, and that the genetic relationships among the selected strains were, to a very limited extent, correlated to their geographical variation. 2016 Society of Chemical Industry.


Chen J.,Shanghai JiaoTong University | Zhang T.,Fujian Academy of Medical Science | Feng L.,Shanghai JiaoTong University | Zhang M.,Shanghai JiaoTong University | And 3 more authors.
Materials Letters | Year: 2013

Highly monodisperse and water-soluble Ribonuclease-A (RNase A) copped-Ag2S quantum dots (QDs) clusters were synthesized in aqueous phase via biomimetic route. The final products were characterized by UV-vis absorption, photoluminescence (PL) spectra, X-ray powder diffraction (XRD) and high-resolution transmission electron microscopy (HR-TEM). Our results revealed that RNase A-Ag2S QDs clusters owned a good photoluminescence property. In addition, RNase A-Ag2S QDs exhibited a good biocompatibility by tetrazolium based colorimetric assay (MTT test). In this work, RNase A not only serves as a stabilizer agent in the formation of Ag 2S QDs to avoid aggregation, but also is a biomolecule to modify the surface of Ag2S QDs to decrease toxicity. These prepared RNase A-Ag2S QDs clusters have great potential applications in molecular imaging in living cells and tissues. © 2012 Elsevier B.V.


Lin Z.-H.,Fujian Agriculture and forestry University | Lin Z.-H.,Fujian Academy of Agricultural science | Qi Y.-P.,Fujian Academy of Medical science | Chen R.-B.,Fujian Academy of Agricultural science | And 2 more authors.
Food Chemistry | Year: 2012

Self-rooted, 10-month-old, uniform tea [Camellia sinensis (L.) O. Kuntze cv. Huangguanyin] plants were supplied with 0, 40, 80, 160, 400 or 1000 μM phosphorus (P) for 17 weeks to determine how P-deficiency affects the quality of green tea. Leaf P concentration increased with increasing P supply. Whole plant dry weight (DW) increased as P supply increased from 0 to 160 μM, then remained little changed with further increasing P supply. The P-deficient green tea displayed decreased concentrations of water extract, total polyphenols, flavonoids, total free amino acids, theanine (Thea) and asparagic acid (Asp) + glutamic acid (Glu), increased concentrations of water soluble sugars, valine (Val), γ-aminobutyric acid (GABA), proline (Pro) and cysteine (Cys), and ratio of total polyphenols to total free amino acids, but unchanged concentrations of total catechins and epigallocatechin gallate (EGCG). In conclusion, the sensory and biochemical qualities of green tea were lowered by P-deficiency. © 2011 Elsevier Ltd. All rights reserved.


Lai Y.,Fujian Medical University | Chen J.,Fujian Medical University | Zhang T.,Fujian Academy of Medical Science | Gu D.,Xiamen University | And 5 more authors.
Journal of Dentistry | Year: 2013

Objectives Fibronectin (FN), an extracellular matrix (ECM) glycoprotein, is a key factor in the compatibility of dental implant materials. Our objective was to determine the optimal dimensions of microgrooves in the transmucosal part of a dental implant, for optimal absorption of plasma FN and expression of cellular FN by human gingival fibroblasts (HGFs). Methods Microgroove titanium surfaces were fabricated by photolithography with parallel grooves: 15 μm, 30 μm, or 60 μm in width and 5 μm or 10 μm in depth. Smooth titanium surfaces were used as controls. Surface hydrophilicity, plasma FN adsorption and cellular FN expression by HGFs were measured for both microgroove and control samples. Results We found that narrower and deeper microgrooves amplified surface hydrophobicity. A 15-μm wide microgroove was the most hydrophobic surface and a 60-μm wide microgroove was the most hydrophilic. The latter had more expression of cellular FN than any other surface, but less absorption of plasma FN than 15-μm wide microgrooves. Variation in microgroove depth did not appear to effect FN absorption or expression unless the groove was narrow (∼15 or 30 μm). In those instances, the shallower depths resulted in greater expression of cellular FN. Conclusions Our microgrooves improved expression of cellular FN, which functionally compensated for plasma FN. A microgroove width of 60 μm and depth of 5 or 10 μm appears to be optimal for the transmucosal part of the dental implant. © 2013 Elsevier Ltd.


Yang L.-T.,Fujian Agriculture and forestry University | Jiang H.-X.,Fujian Agriculture and forestry University | Qi Y.-P.,Fujian Academy of Medical science | Chen L.-S.,Fujian Agriculture and forestry University
Molecular Biology Reports | Year: 2012

The objective was to determine the possible links between the expression levels of genes involved in alternative glycolytic pathways, phosphorus (P) scavenging and recycling and Citrus tolerance to aluminum (Al) and/or P-deficiency. 'Xuegan' (Citrus sinensis) and 'Sour pummelo' (Citrus grandis) seedlings were irrigated for 18 weeks with nutrient solution containing 0 and 1.2 mM AlCl 3 6H 2O 9 0, 50 and 200 μM KH 2PO 4. C. sinensis displayed more tolerant to Al and P-deficiency than C. grandis. Under Al stress, C. sinensis accumulated more Al in roots and less Al in shoots than C. grandis. P concentration was higher in C. sinensis shoots and roots than in C. grandis ones. C. sinensis roots secreted more malate and citrate than C. grandis ones when exposed to Al. Al-induced-secretion of malate and citrate by excised roots from Al-treated seedlings decreased with increasing P supply. Al-induced-secretion of malate and citrate from roots and Al precipitation by P in roots might be responsible for Al-tolerance of C. sinensis. qRT-PCR analysis showed that Al-activated malate transporter (ALMT1), ATP-dependent phosphofructokinase (ATP-PFK), pyrophosphate- dependent phosphofructokinase (PPi-PFK), tonoplast adenosine-triphosphatase subunit A (V-ATPase A), tonoplast pyrophosphatase (V-PPiase), pyruvate kinase (PK), acid phosphatase (APase), phosphoenolpyruvate carboxylase (PEPC), malic enzyme (ME) and malate dehydrogenase (MDH) genes might contribute to the tolerance of Citrus to Al and/or P-deficiency, but any single gene could not explain the differences between the two species. Citrus tolerance to Al and/or P-deficiency might be caused by the coordinated regulation of gene expression involved in alternative glycolytic pathways, P scavenging and recycling. © Springer Science+Business Media B.V. 2011.


Liu Y.Y.,Fujian Medical University | Han J.Y.,Fujian Academy of Medical science | Lin S.C.,Fujian Medical University | Liu Z.Y.,Fujian Medical University | Jiang W.T.,Fujian Medical University
Genetics and Molecular Research | Year: 2014

The aim of this study was to investigate the specific molecular mechanism of the transforming growth factor β (TGF-β)-induced epithelial-mesenchymal transition in a lung cancer cell line, and to provide new ideas for targeting therapy of lung cancer. A549 cells were treated with different concentrations of TGF-β and 5-aza-deoxycytidine (5-aza-dC). The morphological changes after the intervention were observed. The change in the expression of the epithelial marker E-cadherin (E-cad) was detected by Western blot. The proliferation of A549 cells was measured using the MTT assay. Cell movement and invasion capacity was evaluated with the cell scratch test and invasion test. TGF-β induced A549 cells to transform to a mesenchymal cell morphology and downregulated the expression of E-cad, and 5-aza-dC inhibited this phenomenon. Compared with the control group, the number of transmembrane cells was higher and cell migration was markedly increased in the experimental group with continued culture in the presence of 10 ng/mL TGF-β, showing significant differences (P < 0.05). CDH1 gene methylation is involved in TGF-β-induced epithelial-mesenchymal transition in the alveolar epithelial cell line A549. © FUNPEC-RP.


Chen J.-Y.,Fujian Medical University | Chen J.-Y.,Fujian Academy of Medical science | Chen W.-N.,Fujian Medical University | Jiao B.-Y.,Fujian Medical University | And 4 more authors.
BMC Cancer | Year: 2014

Background: The risk of hepatocellular carcinoma (HCC) increases in chronic hepatitis B surface antigen (HBsAg) carriers who often have concomitant increase in the levels of benzo[alpha]pyrene-7,8-diol-9,10-epoxide(±) (BPDE)-DNA adduct in liver tissues, suggesting a possible co-carcinogenesis of Hepatitis B virus (HBV) and benzo[alpha]pyrene in HCC; however the exact mechanisms involved are unclear. Methods: The interaction between hepatitis B spliced protein (HBSP) and microsomal epoxide hydrolase (mEH) was confirmed using GST pull-down, co-immunoprecipitation and mammalian two-hybrid assay; the effects of HBSP on mEH-mediated B[alpha]P metabolism was examined by high performance liquid chromatography (HPLC); and the influences of HBSP on B[alpha]P carcinogenicity were evaluated by bromodeoxyuridine cell proliferation, anchorage-independent growth and tumor xenograft. Results: HBSP could interact with mEH in vitro and in vivo, and this interaction was mediated by the N terminal 47 amino acid residues of HBSP. HBSP could greatly enhance the hydrolysis activity of mEH in cell-free mouse liver microsomes, thus accelerating the metabolism of benzo[alpha]pyrene to produce more ultimate carcinnogen, BPDE, and this effect of HBSP requires the intact HBSP molecule. Expression of HBSP significantly increased the formation of BPDE-DNA adduct in benzo[alpha]pyrene-treated Huh-7 hepatoma cells, and this enhancement was blocked by knockdown of mEH. HBSP could enhance the cell proliferation, accelerate the G1/S transition, and promote cell transformation and tumorigenesis of B[alpha]P-treated Huh-7 hepatoma cells. Conclusions: Our results demonstrated that HBSP could promote carcinogenic effects of B[alpha]P by interacting with mEH and enhancing its hydrolysis activity. © 2014 Chen et al.; licensee BioMed Central Ltd.


Yang L.-T.,Fujian Agriculture and forestry University | Qi Y.-P.,Fujian Academy of Medical science | Chen L.-S.,Fujian Agriculture and forestry University | Sang W.,Fujian Agriculture and forestry University | And 3 more authors.
Environmental and Experimental Botany | Year: 2012

Limited data are available on the amelioration of nitric oxide (NO) on aluminum (Al)-toxicity. Sour pummelo (Citrus grandis) seedlings were irrigated for 18 weeks with nutrient solution containing 0 and 1.2mM AlCl 3·6H 2O×0 and 10μM sodium nitroprusside (SNP, an NO donor). Under Al stress, SNP increased root phosphorus (P) and Al, but decreased shoot Al. Al decreased photosynthesis, maximum quantum yield of primary photochemistry (F v/F m) and total performance index (PI tot,sbs), but increased inactivation of oxygen-evolving complex (OEC), K-band and relative variable fluorescence at I-steps (V I). SNP alleviated Al-induced changes for all these parameters. SNP stimulated Al-induced secretion of malate and citrate by excised roots from Al-treated seedlings, while Al did not increase their contents in roots. Antioxidant system in leaves and roots was up- and down-regulated by Al, respectively. SNP prevented Al-induced accumulation of malondialdehyde (MDA) in roots and leaves. In conclusion, SNP alleviates Al-induced inhibition of growth and impairment of the whole photosynthetic electron transport chain. This occurs through increasing Al-immobilization and P level in roots and Al-induced secretion of malate and citrate from roots, and decreasing Al accumulation in shoots. Thus, the decrease of photosynthesis is prevented. Increased P level and Al-immobilization in roots through SNP may be effected through enhanced secretion of malate and citrate. © 2012 Elsevier B.V..


Zheng Y.,Fujian Medical University | Huang Z.,Fujian Medical University | Huang Z.,Fujian Academy of Medical science | Zhao C.,Fujian Medical University | And 3 more authors.
Microchimica Acta | Year: 2013

We have developed a facile method for the preparation of a gold electrode modified with a flower-like gold nanostructure using potentiostatic electrodeposition. Its formation, morphology, and electrochemical properties were studied by scanning electron microscopy and cyclic voltammetry. The resulting nanostructures possess rough and enlarged surface areas and enable fast electron transfer in the selective and sensitive detection of ascorbic acid (AA) and dopamine (DA) in phosphate-buffered saline without disturbance by common interferents. The differential pulse voltammetry anodic peak currents at approximately -0.03 V and 0.16 V are strongly enhanced in the presence of AA and DA, respectively. The electrode responds linearly to AA in the concentration range from 60 μM to 500 μM, with a limit of detection at 10 μM. The respective data for DA are 1 μM to 150 μM, and the limit of detection is 0.2 μM. © 2013 Springer-Verlag Wien.

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