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Chen J.,Shanghai JiaoTong University | Wang W.,Sun Yat Sen University | Zhang T.,Fujian Academy of Medical Science | Ji J.,Shanghai JiaoTong University | And 5 more authors.
PLoS ONE | Year: 2012

Background: Chronic inflammation plays a causal role in gastric tumor initiation. The identification of predictive biomarkers from gastric inflammation to tumorigenesis will help us to distinguish gastric cancer from atrophic gastritis and establish the diagnosis of early-stage gastric cancer. Phospholipase C epsilon 1 (PLCε1) is reported to play a vital role in inflammation and tumorigenesis. This study was aimed to investigate the clinical significance of PLCε1 in the initiation and progression of gastric cancer. Methodology/Principal Findings: Firstly, the mRNA and protein expression of PLCε1 were analyzed by reverse transcription-PCR and Western blotting in normal gastric mucous epithelial cell line GES-1 and gastric cancer cell lines AGS, SGC7901, and MGC803. The results showed both mRNA and protein levels of PLCε1 were up-regulated in gastric cancer cells compared with normal gastric mucous epithelial cells. Secondly, this result was confirmed by immunohistochemical detection in a tissue microarray including 74 paired gastric cancer and adjacent normal tissues. Thirdly, an independence immunohistochemical analysis of 799 chronic atrophic gastritis tissue specimens demonstrated that PLCε1 expression in atrophic gastritis tissues were down-regulated since PLCε1 expression was negative in 524 (65.6%) atrophic gastritis. In addition, matched clinical tissues from atrophic severe gastritis and gastric cancer patients were used to further confirm the previous results by analyzing mRNA and protein levels expression of PLCε1 in clinical samples. Conclusions/Significances: Our results suggested that PLCε1 protein may be a potential biomarker to distinguish gastric cancer from inflammation lesion, and could have great potential in applications such as diagnosis and pre-warning of early-stage gastric cancer. © 2012 Chen et al.

Chen J.,Shanghai JiaoTong University | Zhang T.,Fujian Academy of Medical Science | Feng L.,Shanghai JiaoTong University | Zhang M.,Shanghai JiaoTong University | And 3 more authors.
Materials Letters | Year: 2013

Highly monodisperse and water-soluble Ribonuclease-A (RNase A) copped-Ag2S quantum dots (QDs) clusters were synthesized in aqueous phase via biomimetic route. The final products were characterized by UV-vis absorption, photoluminescence (PL) spectra, X-ray powder diffraction (XRD) and high-resolution transmission electron microscopy (HR-TEM). Our results revealed that RNase A-Ag2S QDs clusters owned a good photoluminescence property. In addition, RNase A-Ag2S QDs exhibited a good biocompatibility by tetrazolium based colorimetric assay (MTT test). In this work, RNase A not only serves as a stabilizer agent in the formation of Ag 2S QDs to avoid aggregation, but also is a biomolecule to modify the surface of Ag2S QDs to decrease toxicity. These prepared RNase A-Ag2S QDs clusters have great potential applications in molecular imaging in living cells and tissues. © 2012 Elsevier B.V.

Liu Y.Y.,Fujian Medical University | Han J.Y.,Fujian Academy of Medical Science | Lin S.C.,Fujian Medical University | Liu Z.Y.,Fujian Medical University | Jiang W.T.,Fujian Medical University
Genetics and Molecular Research | Year: 2014

The aim of this study was to investigate the specific molecular mechanism of the transforming growth factor β (TGF-β)-induced epithelial-mesenchymal transition in a lung cancer cell line, and to provide new ideas for targeting therapy of lung cancer. A549 cells were treated with different concentrations of TGF-β and 5-aza-deoxycytidine (5-aza-dC). The morphological changes after the intervention were observed. The change in the expression of the epithelial marker E-cadherin (E-cad) was detected by Western blot. The proliferation of A549 cells was measured using the MTT assay. Cell movement and invasion capacity was evaluated with the cell scratch test and invasion test. TGF-β induced A549 cells to transform to a mesenchymal cell morphology and downregulated the expression of E-cad, and 5-aza-dC inhibited this phenomenon. Compared with the control group, the number of transmembrane cells was higher and cell migration was markedly increased in the experimental group with continued culture in the presence of 10 ng/mL TGF-β, showing significant differences (P < 0.05). CDH1 gene methylation is involved in TGF-β-induced epithelial-mesenchymal transition in the alveolar epithelial cell line A549. © FUNPEC-RP.

Yang L.-T.,Fujian Agriculture and forestry University | Jiang H.-X.,Fujian Agriculture and forestry University | Qi Y.-P.,Fujian Academy of Medical Science | Chen L.-S.,Fujian Agriculture and forestry University
Molecular Biology Reports | Year: 2012

The objective was to determine the possible links between the expression levels of genes involved in alternative glycolytic pathways, phosphorus (P) scavenging and recycling and Citrus tolerance to aluminum (Al) and/or P-deficiency. 'Xuegan' (Citrus sinensis) and 'Sour pummelo' (Citrus grandis) seedlings were irrigated for 18 weeks with nutrient solution containing 0 and 1.2 mM AlCl 3 6H 2O 9 0, 50 and 200 μM KH 2PO 4. C. sinensis displayed more tolerant to Al and P-deficiency than C. grandis. Under Al stress, C. sinensis accumulated more Al in roots and less Al in shoots than C. grandis. P concentration was higher in C. sinensis shoots and roots than in C. grandis ones. C. sinensis roots secreted more malate and citrate than C. grandis ones when exposed to Al. Al-induced-secretion of malate and citrate by excised roots from Al-treated seedlings decreased with increasing P supply. Al-induced-secretion of malate and citrate from roots and Al precipitation by P in roots might be responsible for Al-tolerance of C. sinensis. qRT-PCR analysis showed that Al-activated malate transporter (ALMT1), ATP-dependent phosphofructokinase (ATP-PFK), pyrophosphate- dependent phosphofructokinase (PPi-PFK), tonoplast adenosine-triphosphatase subunit A (V-ATPase A), tonoplast pyrophosphatase (V-PPiase), pyruvate kinase (PK), acid phosphatase (APase), phosphoenolpyruvate carboxylase (PEPC), malic enzyme (ME) and malate dehydrogenase (MDH) genes might contribute to the tolerance of Citrus to Al and/or P-deficiency, but any single gene could not explain the differences between the two species. Citrus tolerance to Al and/or P-deficiency might be caused by the coordinated regulation of gene expression involved in alternative glycolytic pathways, P scavenging and recycling. © Springer Science+Business Media B.V. 2011.

Yang L.-T.,Fujian Agriculture and forestry University | Qi Y.-P.,Fujian Academy of Medical Science | Chen L.-S.,Fujian Agriculture and forestry University | Sang W.,Fujian Agriculture and forestry University | And 3 more authors.
Environmental and Experimental Botany | Year: 2012

Limited data are available on the amelioration of nitric oxide (NO) on aluminum (Al)-toxicity. Sour pummelo (Citrus grandis) seedlings were irrigated for 18 weeks with nutrient solution containing 0 and 1.2mM AlCl 3·6H 2O×0 and 10μM sodium nitroprusside (SNP, an NO donor). Under Al stress, SNP increased root phosphorus (P) and Al, but decreased shoot Al. Al decreased photosynthesis, maximum quantum yield of primary photochemistry (F v/F m) and total performance index (PI tot,sbs), but increased inactivation of oxygen-evolving complex (OEC), K-band and relative variable fluorescence at I-steps (V I). SNP alleviated Al-induced changes for all these parameters. SNP stimulated Al-induced secretion of malate and citrate by excised roots from Al-treated seedlings, while Al did not increase their contents in roots. Antioxidant system in leaves and roots was up- and down-regulated by Al, respectively. SNP prevented Al-induced accumulation of malondialdehyde (MDA) in roots and leaves. In conclusion, SNP alleviates Al-induced inhibition of growth and impairment of the whole photosynthetic electron transport chain. This occurs through increasing Al-immobilization and P level in roots and Al-induced secretion of malate and citrate from roots, and decreasing Al accumulation in shoots. Thus, the decrease of photosynthesis is prevented. Increased P level and Al-immobilization in roots through SNP may be effected through enhanced secretion of malate and citrate. © 2012 Elsevier B.V..

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