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Ying J.F.,Zhejiang University | Ying J.F.,Fujian Academy of Agriculture Science | Xm C.,Zhejiang University | Cheng L.A.,Zhejiang University
International Journal of Environmental Research

Fifteen kinds of local wetland plants in Taihu Lake region of China were investigated on their respective growth conditions, biomass allocations and nutrient uptake capacities. The test plants were cultured in 3 L nutrient solutions for 28 days with NH 4 +-N, NO 2--N and P concentrations of 38.5, 132.8 and 10 mg/L, respectively. Mean species total biomass ranged from 1.2-21.6 g/plant with above/below ground ratios (AG:BG) between 1.7-5.5. Mean NH 4 +-N, NO 2-N and P uptake rates ranged from 3.7-14.3 mg/day (8.0-49.4% of NH 4 +-N supplied), 17.8-59.4 mg/day (17.8-59.6%) Of NO 2-N supplied and 1.71-4.61 mg /day (24.1-61.5% of P supplied), respectively. Plant tissue N and P concentrations ranged between 28.2-606.1 mg N /plant and 4.1-53.1 mg P/plant, with AG:BG ratio between 1.7-7.0 and 1.6-4.6, respectively. The accumulation of N and P in plant tissues was significantly correlated with plant biomass and root surface area. Among the different species, C. generalis, T. larifolia, T. dealbata and L. salicaria were better than the other plants in above- and below-ground biomass, root surface area, nutrient uptake and storage rates. T. dealbata and L. salicaria were more suitable for year-round application in this area due to their tolerance of coldness. Source

Lin F.,Fujian Academy of Agriculture Science
Wei sheng wu xue bao = Acta microbiologica Sinica

To study the immunosuppression effect on the thymus of muscovy ducks after infected with muscovy duck reovirus (MDRV) and H9 influenza virus (AIV). After 8-day-old birds were infected with MDRV or H9 AIV, or both, the morbidity and mortality were totaled, the morphology and ultra-structure of the thymus were observed, proliferation ability of thymus cell were detected and the virus distrubition were detected by RT-PCR. After H9 AIV infection, The morbidity was low (10%) and without death. No obvious pathological change was observed on the thymus, whereas the proliferation ability of thymus cell was obviously suppressed. After MDRV infection, The birds grew slow, the morbidity was 80% and mortality was 50%. Thymus was atrophy appearing local necrosis and proliferation ability of thymus cell was obviously suppressed. After co-infection with MDRV and H9 AIV, the birds grew even slower growth. The morbidity was 90% and mortality was 70%. The thymus was atrophy appearing the lymphopenia and local necrosis and proliferation ability of thymus cell was also more obviously suppressed than MDRV infection. Virus duration time and detection ratio in co-infection group were more than in AIV and MDRV group. H9 AIV could lead to minor immunosuppression and MDRV could cause serious immuno-suppression. H9 AIV could aggravate the immunosuppression of thymus after co-infected with MDRV, so MDRV and H9 AIV infection had synergic effect on immunosuppression of the thymus. Source

Liu M.,Chinese Academy of Agricultural Sciences | Chen S.,Fujian Academy of Agriculture Science | Chen Y.,Chinese Academy of Agricultural Sciences | Liu C.,Chinese Academy of Agricultural Sciences | And 3 more authors.
Journal of Clinical Microbiology

An outbreak of egg drop disease occurred in many chicken and goose farms in China in 2011. By using an NS5-specific reverse transcriptase PCR (RT-PCR), we found that 56% of chicken and 38% of goose samples were positive for Tembusu-like virus (TMUV). Isolates showed high sequence homology to duck TMUVs, and chickens and geese showed signs of egg drop disease after experimental infection with duck TMUV. Our data suggest TMUV has adapted in domestic birds. Copyright © 2012, American Society for Microbiology. All Rights Reserved. Source

Jiang F.Y.,Zhejiang University | Jiang F.Y.,Fujian Academy of Agriculture Science | Chen X.,Zhejiang University | Luo A.C.,Zhejiang University
Chemistry and Ecology

The biomass, root morphology and nutrient uptake capacities of 15 species of local wetland plants were investigated in hydroponic culture. The wetland plants were exposed to 38.5 mg·L-1 of NH+ 4-N, 132.8 mg·L-1 of NO- 3-N and 10 mg·L-1 of dissolved inorganic P for 28 days. Mean total biomass of the 15 species ranged from 1.2 to 21.6 g plant-1, with above/below ground ratios (AG:BG) in the range 1.7-5.5. Mean NH+ 4-N, NO- 3-N uptake rates ranged from 3.7 to 14.3 mg N·day-1 (accounting for 8.0-49.4% of the NH+ 4 -N supply) and 17.8 to 59.4 mg N·day-1 (17.8-59.6% of the NO- 3-N supply). Mean P uptake rate ranged from 1.71 to 4.61 mg P·day-1 (24.1-61.5% of the P supply). The N and P concentrations in plant tissues ranged from 28.2 to 606.1 mg N·plant-1 and 4.1 to 53.1 mg P·plant-1, withAG:BG ratio in the range 1.7-7.0 and 1.6-4.6, respectively. The accumulation of N and P in plant tissues was both significantly correlated with plant biomass and root surface area. Among the different species, Canna generalis, Typha latifolia, Thalia dealbata and Lythrum salicaria had greater above- and below-ground biomass, larger root surface area, and greater nutrient uptake and storage rates than the other plants. Our results suggest (or indicate) that the selection of plant species suitable for constructed wetlands can be based on plant biomass and root surface area. © 2011 Taylor & Francis. Source

Zhang Y.,Xiamen University | Zhang Y.,National Institute of Allergy and Infectious Diseases | Qi Y.,Xiamen University | Li J.,Xiamen University | And 5 more authors.
Parasitology Research

Of all the parasitic diseases, malaria is the number one killer. Despite tremendous efforts in disease control and research, nearly a million people, primarily children, still die from the disease each year, partly due to drug resistance and the lack of an effective vaccine. Many parasite antigens have been identified and evaluated for vaccine development; however, none has been approved for human use. Antigenic variation, complex life cycle, and inadequate understanding of the mechanisms of parasite- host interaction and of host immune response all contribute to the lack of an effective vaccine for malaria control. In a recent search of genome-wide polymorphism in Plasmodium falciparum, several molecules were found to be recognized by sera from patients infected with the P. falciparum parasite. Here, we have expressed a 350-amino acid N terminus from one of the homologous candidate antigen genes from the rodent malaria parasite Plasmodium yoelii (Py01157, a putative dentin phosphorin) in bacteria and evaluated the immune response and protection generated after immunization with the recombinant protein.We showed that the recombinant protein was recognized by sera from both mice and humans infected with malaria parasites. Partial protection was observed after challenge with non-lethal P. yoelii 17XNL but not with the lethal P. yoelii 17XL parasite. Further tests using a full-length protein or the conserved C terminus may provide additional information on whether this protein has the potential for being a malaria vaccine. © Springer-Verlag 2012. Source

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