Fujian Academy of Agriculture Science

Fuan, China

Fujian Academy of Agriculture Science

Fuan, China
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PubMed | Fujian Academy of Agricultural science and Fujian Academy of Agriculture science
Type: Journal Article | Journal: Archives of virology | Year: 2016

Many mule duck and Cherry Valley duck flocks in different duck-producing regions of China have shown signs of an apparently new disease designated short beak and dwarfism syndrome (SBDS) since 2015. The disease is characterized by dyspraxia, weight loss, a protruding tongue, and high morbidity and low mortality rates. In order to characterize the etiological agent, a virus designated SBDSV M15 was isolated from allantoic fluid of dead embryos following serial passage in duck embryos. This virus causes a cytopathic effect in duck embryo fibroblast (DEF) cells. Using monoclonal antibody diagnostic assays, the SBDSV M15 isolate was positive for the antigen of goose parvovirus but not Muscovy duck parvovirus. A 348-bp (2604-2951) VP1gene fragment was amplified, and its sequence indicated that the virus was most closely related to a Hungarian GPV strain that was also isolated from mule ducks with SBDS disease. A similar disease was reproduced by inoculating birds with SBDSV M15. Together, these data indicate that SBDSV M15 is a GPV-related parvovirus causing SBDS disease and that it is divergent from classical GPV isolates.

Xing S.,Fujian Agriculture and forestry University | Xing S.,Griffith University | Chen C.,Fujian Academy of Agriculture science | Chen C.,Griffith University | And 4 more authors.
Journal of Soils and Sediments | Year: 2010

Purpose Soil soluble organic nitrogen (SON) is considered as a sensitive indicator of soil nitrogen (N) status and plays an important role in N cycling in forest ecosystems. Most work on forest soil SON to date has been conducted in temperate areas. The information about soil SON pools and dynamics in tropical and subtropical areas is limited. The aim of this study was to investigate the effects of different forest types on soil SON availability and associated microbial properties. Materials and methods Soil samples were collected at two depths (0-20 and 20-40 cm) from adjacent 12-year-old plantation forests of coniferous Chinese fir (Cunninghamia lanceolata (Lamb) Hook; Coniferous Fir), coniferous-broadleaf mixed species of broadleaf Nagai Podocarpus and coniferous Pond cypress (mixed species), and broadleaf Phoebe nanmu (broadleaf Phoebe) in subtropical China. Three extraction methods (water, hot water, and 2 M KCl) were used for measurements of SON. Soil microbial biomass carbon (C) and N and activities of urease, protease, and L-asparaginase were also measured. Results and discussion Hot water and KCl extractable SON pools were greater in the surface soils (0-20 cm) under the broadleaf Phoebe than under the Coniferous Fir, while those under the mixed species were intermediate. The microbial biomass C and N as well as activities of protease and urease were higher in the soils under the broadleaf Phoebe and mixed species than under the Coniferous Fir. Soluble organic C and SON pools were positively related to soil total C and N and microbial biomass C and microbial biomass N. Activities of urease, protease, and asparaginase were highly correlated with SONw, SONhw, and SONKCl. This work has demonstrated that forest type had significant impacts on the pool size of soil SON. This may be attributed to the different quantity and quality of organic inputs from different forest systems. Positive relationships between microbial biomass and enzyme activities with the SON pools clearly demonstrated that microbial communities play a vital role in the dynamics of soil SON in forest ecosystems. Conclusions It is recommended that soil SON measured by hot water extraction be incorporated into the routine measurement of soil available N in forest ecosystems. Further study on the role of specific microbial functional groups in the SON transformation and the chemical nature and seasonal dynamics of SON pools in forest ecosystems is warranted. © Springer-Verlag 2009.

PubMed | Fujian Academy of Agriculture science and Fujian Agriculture and forestry University
Type: | Journal: Applied biochemistry and biotechnology | Year: 2016

This study aimed to develop biocontrol Bacillus and explore bacterial biocontrol substances. According to the blood agar test, strain FJAT-14262 was screened as a biosurfactant-producer. The biosurfactant-producing ability of FJAT-14262 was further confirmed by the oil spreading tests because of its amphipathic character. Furthermore, its fermentation supernatant could decrease the surface tension from 74.1 to 32.7mNm

Jiang F.Y.,Zhejiang University | Jiang F.Y.,Fujian Academy of Agriculture Science | Chen X.,Zhejiang University | Luo A.C.,Zhejiang University
Chemistry and Ecology | Year: 2011

The biomass, root morphology and nutrient uptake capacities of 15 species of local wetland plants were investigated in hydroponic culture. The wetland plants were exposed to 38.5 mg·L-1 of NH+ 4-N, 132.8 mg·L-1 of NO- 3-N and 10 mg·L-1 of dissolved inorganic P for 28 days. Mean total biomass of the 15 species ranged from 1.2 to 21.6 g plant-1, with above/below ground ratios (AG:BG) in the range 1.7-5.5. Mean NH+ 4-N, NO- 3-N uptake rates ranged from 3.7 to 14.3 mg N·day-1 (accounting for 8.0-49.4% of the NH+ 4 -N supply) and 17.8 to 59.4 mg N·day-1 (17.8-59.6% of the NO- 3-N supply). Mean P uptake rate ranged from 1.71 to 4.61 mg P·day-1 (24.1-61.5% of the P supply). The N and P concentrations in plant tissues ranged from 28.2 to 606.1 mg N·plant-1 and 4.1 to 53.1 mg P·plant-1, withAG:BG ratio in the range 1.7-7.0 and 1.6-4.6, respectively. The accumulation of N and P in plant tissues was both significantly correlated with plant biomass and root surface area. Among the different species, Canna generalis, Typha latifolia, Thalia dealbata and Lythrum salicaria had greater above- and below-ground biomass, larger root surface area, and greater nutrient uptake and storage rates than the other plants. Our results suggest (or indicate) that the selection of plant species suitable for constructed wetlands can be based on plant biomass and root surface area. © 2011 Taylor & Francis.

Ying J.F.,Zhejiang University | Ying J.F.,Fujian Academy of Agriculture Science | Xm C.,Zhejiang University | Cheng L.A.,Zhejiang University
International Journal of Environmental Research | Year: 2011

Fifteen kinds of local wetland plants in Taihu Lake region of China were investigated on their respective growth conditions, biomass allocations and nutrient uptake capacities. The test plants were cultured in 3 L nutrient solutions for 28 days with NH 4 +-N, NO 2--N and P concentrations of 38.5, 132.8 and 10 mg/L, respectively. Mean species total biomass ranged from 1.2-21.6 g/plant with above/below ground ratios (AG:BG) between 1.7-5.5. Mean NH 4 +-N, NO 2-N and P uptake rates ranged from 3.7-14.3 mg/day (8.0-49.4% of NH 4 +-N supplied), 17.8-59.4 mg/day (17.8-59.6%) Of NO 2-N supplied and 1.71-4.61 mg /day (24.1-61.5% of P supplied), respectively. Plant tissue N and P concentrations ranged between 28.2-606.1 mg N /plant and 4.1-53.1 mg P/plant, with AG:BG ratio between 1.7-7.0 and 1.6-4.6, respectively. The accumulation of N and P in plant tissues was significantly correlated with plant biomass and root surface area. Among the different species, C. generalis, T. larifolia, T. dealbata and L. salicaria were better than the other plants in above- and below-ground biomass, root surface area, nutrient uptake and storage rates. T. dealbata and L. salicaria were more suitable for year-round application in this area due to their tolerance of coldness.

Wang S.,Fujian Academy of Agriculture science | Cheng X.,Fujian Academy of Agriculture science | Chen S.,Fujian Academy of Agriculture science | Lin F.,Fujian Academy of Agriculture science | And 2 more authors.
Archives of Virology | Year: 2015

To investigate the possible role of recombination in the evolution of Muscovy duck parvovirus (MDPV) and goose parvovirus (GPV) in Taiwan, we analyzed a potentially significant recombination event that occurred only in GPV by comparing thirteen complete sequences of the capsid gene VP2 of GPV and MDPV. The recombination event occurred between GPV strain 06-0239 as the minor parent and strains 99-0808 as the major parent, which resulted in the GPV recombinant V325/TW03. GPV V325/TW03 is likely to represent a new genotype among the Taiwanese GPV strains. This represents the first evidence that intergenotype recombination within the VP2 gene cluster contributes to the genetic diversity of the VP2 genes of Taiwanese GPV field strains. © 2015, Springer-Verlag Wien.

Liu M.,Chinese Academy of Agricultural Sciences | Chen S.,Fujian Academy of Agriculture science | Chen Y.,Chinese Academy of Agricultural Sciences | Liu C.,Chinese Academy of Agricultural Sciences | And 3 more authors.
Journal of Clinical Microbiology | Year: 2012

An outbreak of egg drop disease occurred in many chicken and goose farms in China in 2011. By using an NS5-specific reverse transcriptase PCR (RT-PCR), we found that 56% of chicken and 38% of goose samples were positive for Tembusu-like virus (TMUV). Isolates showed high sequence homology to duck TMUVs, and chickens and geese showed signs of egg drop disease after experimental infection with duck TMUV. Our data suggest TMUV has adapted in domestic birds. Copyright © 2012, American Society for Microbiology. All Rights Reserved.

Lin F.,Fujian Academy of Agriculture science
Wei sheng wu xue bao = Acta microbiologica Sinica | Year: 2011

To study the immunosuppression effect on the thymus of muscovy ducks after infected with muscovy duck reovirus (MDRV) and H9 influenza virus (AIV). After 8-day-old birds were infected with MDRV or H9 AIV, or both, the morbidity and mortality were totaled, the morphology and ultra-structure of the thymus were observed, proliferation ability of thymus cell were detected and the virus distrubition were detected by RT-PCR. After H9 AIV infection, The morbidity was low (10%) and without death. No obvious pathological change was observed on the thymus, whereas the proliferation ability of thymus cell was obviously suppressed. After MDRV infection, The birds grew slow, the morbidity was 80% and mortality was 50%. Thymus was atrophy appearing local necrosis and proliferation ability of thymus cell was obviously suppressed. After co-infection with MDRV and H9 AIV, the birds grew even slower growth. The morbidity was 90% and mortality was 70%. The thymus was atrophy appearing the lymphopenia and local necrosis and proliferation ability of thymus cell was also more obviously suppressed than MDRV infection. Virus duration time and detection ratio in co-infection group were more than in AIV and MDRV group. H9 AIV could lead to minor immunosuppression and MDRV could cause serious immuno-suppression. H9 AIV could aggravate the immunosuppression of thymus after co-infected with MDRV, so MDRV and H9 AIV infection had synergic effect on immunosuppression of the thymus.

Ji J.,Fujian Academy of Agriculture science | Lin T.,Fujian Academy of Agriculture science | Zhang Y.,Fujian Academy of Agriculture science | Sun L.,Fujian Academy of Agriculture science | And 3 more authors.
Systematic and Applied Acarology | Year: 2013

The ability of predatory mites to endure starvation or low humidity is very important for them to maintain their populations in unfavorable environments. The effects of starvation and humidity on the development and survival of Amblyseius (Typhlodromips) swirskii, Amblyseius eharai and Agistemus exsertus were studied in the laboratory. The results showed that most eggs of A. eharai and A. exsertus could not hatch at relative humidities (RH) lower than 60%. Only A. swirskii larvae could develop into protonymph stage without prey at 60-100%RH among three species. When the prey was absent, protonymphs or gravid females of A. swirskiisurvived significantly longer than those of A. eharai at 60-100%RH, respectively. Furthermore, at lower humidities (60-70%RH) similar case was observed in the virgin female stage. In addition, nymphs and females of the two phytoseiid mites survived significantly longer than those of A. exsertus at 60-100%RH. It indicated that A. swirskii might be a stronger competitor relative to A. eharai or A. exsertus under starvation and low humidity conditions. © 2013 Systematic & Applied Acarology Society.

Ji J.,Fujian Academy of Agriculture science | Lin T.,Fujian Academy of Agriculture science | Zhang Y.X.,Fujian Academy of Agriculture science | Lin J.Z.,Fujian Academy of Agriculture science | And 2 more authors.
Systematic and Applied Acarology | Year: 2013

In this study, the developmental duration and life table of Amblyseius (Typhlodromips) swirskii and Amblyseius eharai with the prey Panonychus citri were studied at 25±1°C. The results showed that the developmental duration of the larval stage and a whole generation of A. swirskii were significantly shorter than those of A. eharai, but no significant differences in developmental duration were observed in other stages. In addition, the larva of A. swirskii could develop into a protonymph without any prey, but prey was necessary for larval development of A. eharai. The fecundity of the adult female of A. eharai was significantly higher than that of A. swirskii. The net reproductive rate (Ro), intrinsic rate of increase (rm) and the finite rate of increase (λ) of A. eharai were higher than those of A. swirskii. The results indicated that A. eharai might be a better competitor compared with A. swirskii according to the parameters of life table. © 2013 Systematic & Applied Acarology Society.

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