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Huang W.,Fujian Academy of Agricultural science Fujian Key Laboratory of Precise Measurement of Agricultural | Song Y.,Fujian Academy of Agricultural science Fujian Key Laboratory of Precise Measurement of Agricultural | Yu H.,Fujian Academy of Agricultural science Fujian Key Laboratory of Precise Measurement of Agricultural | Tian B.,Fujian Normal University
Journal of Chinese Institute of Food Science and Technology | Year: 2014

In this paper, ultrasonic, heating, acid and alkali treatments were studied to improve the insoluble of corn protein. The preparation of corn anti-oxidation peptide with enzymolysis method was also studied. The results showed that the optimum pretreatment conditions were corn protein solution pH 11, ultrasonic power 45 W, temperature of ultrasonic treatment 60℃, ultrasonic pretreatment time 30 min. Under the optimum conditions, the yield of soluble corn protein was increased significantly. Six enzyemes were respectively used to preparation of corn anti-oxidation peptide for screening the best one. Alcalase was detected as the optimum enzyeme. Single factor experiment and response surface methodology were used for optimizing the enzymatic hydrolysis conditions in this study. The optimum hydrolysis conditions to achieve the maximum antioxidant ability were as follows: substrate concentration 10.0 g/100 mL, enzyme concentration of 2 806 U/g, pH9.0, Enzymolysis temperature of 56.0℃, and enzymolysis time of 185 min. Under these conditions, DPPH free radical scavenging rate of the hydrolysate liguid could reached up to (85.40±0.27)%, which coincide with predictive values with the relative error of less than 1%. Source


Huang W.,Fujian Academy of Agricultural science Fujian Key Laboratory of Precise Measurement of Agricultural | Song Y.,Fujian Academy of Agricultural science Fujian Key Laboratory of Precise Measurement of Agricultural | Yu H.,Fujian Academy of Agricultural science Fujian Key Laboratory of Precise Measurement of Agricultural | Tian B.,Fujian Normal University
Journal of the Chinese Cereals and Oils Association | Year: 2013

To establish Tricine-sodium dodecyl sulphate-polyacrylamide gel electrophoresis system(Tricine-SDS-PAGE)for peptides from hydrolyzed soybean proteins, the experimental condition of Tricine-SDS-PAGE had been optimized; various types of gel, different sample loading buffers and loading quantities, different gel staining methods also had been studied. The results showed that acrylamide/bisacrylamide concentration(T=18%)and crosslinking(C=5%)containing 30% ethylene glycol could separate soybean peptides down to 1.7 ku; using sample loading buffer containing 30% glycerol was better than other sample loading buffers; the suitable loading quantity was 20 μg; Coomassie Blue staining G-250 enabled peptides to be stained and distained rapidly, and also enhanced detection sensitivity. Meanwhile, the improved Tricine-SDS-PAGE system can be used to separate and analyze molecular weight peptides from hydrolyzed soybean proteins by different proteases. The result indicates that the optimized Tricine-SDS-PAGE will be able to obtain the higher resolution peptide maps of hydrolyzed soybean proteins. Source


Song Y.,Fujian Academy of Agricultural science Fujian Key Laboratory of Precise Measurement of Agricultural | Huang W.,Fujian Academy of Agricultural science Fujian Key Laboratory of Precise Measurement of Agricultural | Yao Q.,Fujian Academy of Agricultural science Fujian Key Laboratory of Precise Measurement of Agricultural | Lin X.,Fujian Academy of Agricultural science Fujian Key Laboratory of Precise Measurement of Agricultural | Lin Q.,Fujian Academy of Agricultural science Fujian Key Laboratory of Precise Measurement of Agricultural
Journal of the Chinese Cereals and Oils Association | Year: 2013

In order to improve the aquatic feed processing quality of hydrolyzed soybean meal, single factor experiment and orthogonal test design were used for optimization of the bi-enzymatic hydrolysis conditions to produce aquatic feed oligo-peptide soybean meal in this study. Based on the evaluation of the production of soybean oligopeptide, the optimum hydrolysis conditions were designated as substrate concentration of 80 g/L, the ratio of the two enzyme (Alcalase: Flavourzyme) of 2:1, protease amount of 3000 U/g, hydrolysis temperature of 61°C, pH 8.0 and reaction time of 7.5 h. Finally, a yield of soybean oligo-peptide of 38.16% and a DH of 22.69% were obtained. The resultant oligopeptide soybean product had a better processing quality with a greater affinity with -starch and a less leaching loss of product of (2.5±0.7)%. The viscoelasticity and leaching losses of oligo-peptide product were able to meet the processing requirement of aquatic feeds. The results showed that the yield of oligo-peptide was a better indication for controlling the process of soybean meal hydrolysis than the DH (degree of hydrolysis) to produce a high-quality aquatic feed oligo-peptide soybean meal. Source

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