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Chiu D.,Fuda Cancer Hospital at Guangzhou | Chiu D.,Chinese Academy of Sciences | Niu L.,Fuda Cancer Hospital at Guangzhou | Niu L.,Chinese Academy of Sciences | And 14 more authors.
Cryobiology | Year: 2010

Objective: This study was designed to basic information concerning the efficacy and safety of cryosurgery for pancreatic cancer. Fifteen healthy pigs were used to perform biochemical analysis and histological assessment. Methods: Following anesthesia and laparotomy, an argon-helium cryoprobe was inserted into the pancreas. The introduction of argon gas induced a rapid decrease in temperature to -160°C (Group I, 5 pigs) or -110 °C (Group II, 5 pigs), respectively, resulting in ice-ball formation of 15-20. mm diameter after 5. min. Following freezing, helium gas was circulated in the probe tip to increase the temperature to 10-20 °C over 3. min to thaw. The freeze/thaw cycle was then repeated. Group III (3 pigs) had a cryoprobe inserted, but without freezing, and Group IV (2 pigs) included untreated or normal control animals. Levels of serum amylase (AMY), IL-6 and C-RP were measured prior to freezing and for 7. days following the procedure. All pigs were euthanized 7. days post-treatment and pancreases were examined histologically. Results: Neither hyperaemia, edema or hemorrhage were observed in the un-frozen parts of the pancreas. Histological assessment revealed a significant level of necrosis in the central and lateral regions of the tissue frozen within the ice-ball. All cellular ultrastructure was destroyed and only observable as a few of remaining nuclei with broken crests and degranulated mitochondria and rough endoplasmic reticulum. There was a significant increase of serum AMY levels for a brief period in both " deep frozen" and the " shallow frozen" groups. However, the AMY also increased in two pigs in the " normal control" group and one pig from the " inserted cryoprobe without freeze" control group. All experimental pigs appeared healthy until the sacrifice time. Conclusion: Cryosurgery is a safe and effective ablative procedure for pancreatic tissue resulting in minimal complications. © 2010 Elsevier Inc. Source

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