Fu Zhou University

Fuzhou, China

Fu Zhou University

Fuzhou, China

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Zhang F.,Fu Zhou University | Hu C.,Fu Zhou University
Construction and Building Materials | Year: 2014

The crumb rubber (CR) compound modified asphalt with improved tenacity and high- or low-temperature properties was prepared by the addition of styrene-butadiene-styrene (SBS) and the stability of CR/SBS-modified (CRSM) asphalt was improved by the addition of sulfur. Rheological testing demonstrated the improved high-temperature performance of modified binders and indicated the susceptibility of vulcanizated binder to dynamic shear. Morphology observation showed the compatibility between CR and asphalt was improved greatly by vulcanization or ageing. Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) analysis showed the characteristics and distribution of the major functional groups of modified asphalt before and after ageing. In thermal analysis, DSC curve showed the effect of ageing and modifier on the molecule weight distribution and constituents of asphalt. The thermal stability and susceptibility of each binder to thermal decomposition were evaluated by using TG and DTG curves, which indicated the structural characteristics of binder and demonstrated the conclusion drawn by DSC analysis further. © 2014 Elsevier B.V. All rights reserved.


Du J.Y.,Fu Zhou University | Chen L.R.,Fu Zhou First Hospital | Liu S.,Fu Zhou University | Lin J.H.,Fu Zhou University | And 3 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2016

The rare N-unsubstituted glucosamine (GlcNH3 +) residues in heparan sulfate (HS) have important biological and pathophysiological roles. In this study, a high-resolution method for the separation and analysis of N-unsubstituted disaccharides of heparin/HS is described. Four N-unsubstituted disaccharides, together with eight N-substituted species, can be well-separated by ion-pair reverse-phase ultra-performance liquid chromatography. Each disaccharide can then be detected and its relative abundance quantified using electrospray ionization mass spectrometry in the negative mode. Because of its high sensitivity, without interference from proteins and other sample impurities, this method is particularly useful in the analysis of low content GlcNH3 + residues in small amounts of biological materials, eg. sera, tissue and cell culture-derived samples. This would lead to a better understanding of the biological origin of GlcNH3 + residues and their increasingly important function in human health and disease. © 2016 Elsevier B.V.


Liang Q.T.,Fu Zhou University | Du J.Y.,Fu Zhou University | Fu Q.,Fu Zhou University | Lin J.H.,Fu Zhou University | Wei Z.,Fu Zhou University
Glycoconjugate Journal | Year: 2015

The rare N-unsubstituted glucosamine (GlcNH3 +) residues in heparan sulfate (HS) have important biological and pathophysiological roles. Therefore, the ability to chemically generate a series of oligosaccharides, which have a similar structure to the naturally-occurring, GlcNH3 + - containing oligosaccharides from HS, would greatly contribute to investigating their natural role in HS. In this study, a hexasaccharide library that possess GlcNH3 + residues were prepared from the chemical modification of the fully sulfated dp6. Chemical reaction conditions were optimized to generate different pattern of GlcNH3 + - containing oligosaccharides, then the structure of the library was detected by high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC/MS-ITTOF) analysis. EIC/MS and MS2 analysis showed different fragmentation patterns of dp6s with different GlcNH3 + residues. This provides a foundation for further identification and quantification of GlcNH3 + - oligosaccharides by mass spectrum analysis. © 2015 Springer Science+Business Media New York.


Liang Q.T.,Fu Zhou University | Xiao X.M.,Fu Zhou University | Lin J.H.,Fu Zhou University | Wei Z.,Fu Zhou University
Glycobiology | Year: 2015

The rare N-unsubstituted glucosamine (GlcNH3 +) residues in heparan sulfate (HS) have important biological and pathophysiological roles. Because of their low natural abundance, the use of chemically generated, structurally defined, N-unsubstituted heparin/HS oligosaccharides can greatly contribute to the investigation of their natural role in HS. However, the sequencing of mixtures of chemically generated oligosaccharides presents major challenges due to the difficulties in separating isomers and the available detection methods. In this study, we developed and validated a simple and sensitive method for the sequence analysis of N-unsubstituted heparin/HS oligosaccharides. This protocol involves pH 4 nitrous acid (HNO2) degradation, size-exclusion HPLC and ion-pair reversed-phase liquid chromatography-ion trap/time-of-flight mass spectrometry (IPRP-LC-ITTOF MS). We unexpectedly found that absorbance at 232 nm (normally used for specific detection of C4-C5 unsaturated oligosaccharides) was, in most cases, still sufficiently sensitive to also simultaneously detect saturated oligosaccharides during HPLC, thus simplifying the positional analysis of GlcNH3 + residues. The IPRP-LC-ITTOF MS system can supply further structural information leading to full sequence determination of the original oligosaccharide. This new methodology has been used to separate and sequence a variety of chemically generated, N-unsubstituted dp6 species containing between 1 and 3 GlcNH3 + residues per oligosaccharide in different positional combinations. This strategy offers possibilities for the sequencing of natural N-unsubstituted oligosaccharides from HS and should also be applicable, with minor modification, for sequencing at N-sulfated residues using alternative pH 1.5 HNO2 scission. © 2015 The Author 2015. Published by Oxford University Press. All rights reserved.


Zhang F.,Fu Zhou University | Hu C.,Fu Zhou University
Journal of Thermal Analysis and Calorimetry | Year: 2015

Crumb rubber (CR)/waste plastic compound modified asphalts were prepared by the addition of CR and waste plastic including waste polypropylene, low-density polyethylene, linear low-density polyethylene (LLDPE). The physical properties of each modified asphalt were studied and compared, and LLDPE was confirmed as the right modifier in enhancing the high-temperature performance. Meanwhile, dioctyl phthalate (DP) as a plasticizer was used further in improving low-temperature properties. On the basis of the optimal proportion of CR/LLDPE/DP modified asphalt, rheological tests were used to study the high- and low-temperature properties and structural characteristics of modified binder. Fourier transform infrared spectroscopy was used to investigate the modification mechanism of each modifier. Morphology observation was used to study the effect of modifier and ageing on the morphological characteristics of asphalt. Thermal analysis was adopted to study the thermodynamic characteristics and constituents of each modified binder. © 2015 Akadémiai Kiadó, Budapest, Hungary


Wei Z.,Fu Zhou University | Wei Z.,University of Manchester | Deakin J.A.,University of Manchester | Blaum B.S.,University of Edinburgh | And 3 more authors.
Glycoconjugate Journal | Year: 2011

The rare N-unsubstituted glucosamine (GlcNH3 +) residues in heparan sulfate (HS) have important biological and pathophysiological roles. However, it is difficult to prepare naturally-occuring, GlcNH3 +-containing oligosaccharides from HS because of their low abundance, as well as the inherent problems in both excising and identifying them. Therefore, the ability to chemically generate a series of structurally-defined oligosaccharides containing GlcNH 3 + residues would greatly contribute to investigating their natural role in HS. In this study, a series of heparin/HS oligosaccharides, from dp6 up to dp16 in length that possess internal GlcNH 3 + residues were prepared by a combination of chemical modification and heparinase I digestion. Purification and structural analysis of the major species derived from the octa-to dodeca-saccharide size fractions indicated the introduction of between 1 and 3 internal GlcNH3 + residues per oligosaccharide. In addition, a GlcNH3 + residue was selectively introduced into an internal position in a tetrasaccharide species by direct chemical modification. This selectivity has potential as an alternative procedure for preparing internally-modified oligosaccharides of various lengths. The utility of such oligosaccharides was demonstrated by a comparison of the binding of three different tetrasaccharide species containing 0, 1 and 2 free amino groups to the NK1 truncated variant of hepatocyte growth factor/scatter factor. © The Author(s) 2011.


PubMed | Fu Zhou First Hospital and Fu Zhou University
Type: | Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences | Year: 2016

The rare N-unsubstituted glucosamine (GlcNH3(+)) residues in heparan sulfate (HS) have important biological and pathophysiological roles. In this study, a high-resolution method for the separation and analysis of N-unsubstituted disaccharides of heparin/HS is described. Four N-unsubstituted disaccharides, together with eight N-substituted species, can be well-separated by ion-pair reverse-phase ultra-performance liquid chromatography. Each disaccharide can then be detected and its relative abundance quantified using electrospray ionization mass spectrometry in the negative mode. Because of its high sensitivity, without interference from proteins and other sample impurities, this method is particularly useful in the analysis of low content GlcNH3(+) residues in small amounts of biological materials, eg. sera, tissue and cell culture-derived samples. This would lead to a better understanding of the biological origin of GlcNH3(+) residues and their increasingly important function in human health and disease.


PubMed | Fu Zhou University
Type: Journal Article | Journal: Glycoconjugate journal | Year: 2011

The rare N-unsubstituted glucosamine (GlcNH (3)(+)) residues in heparan sulfate (HS) have important biological and pathophysiological roles. However, it is difficult to prepare naturally-occuring, GlcNH(3)(+)-containing oligosaccharides from HS because of their low abundance, as well as the inherent problems in both excising and identifying them. Therefore, the ability to chemically generate a series of structurally-defined oligosaccharides containing GlcNH(3)(+) residues would greatly contribute to investigating their natural role in HS. In this study, a series of heparin/HS oligosaccharides, from dp6 up to dp16 in length that possess internal GlcNH(3)(+) residues were prepared by a combination of chemical modification and heparinase I digestion. Purification and structural analysis of the major species derived from the octa- to dodeca-saccharide size fractions indicated the introduction of between 1 and 3 internal GlcNH(3)(+) residues per oligosaccharide. In addition, a GlcNH(3)(+) residue was selectively introduced into an internal position in a tetrasaccharide species by direct chemical modification. This selectivity has potential as an alternative procedure for preparing internally-modified oligosaccharides of various lengths. The utility of such oligosaccharides was demonstrated by a comparison of the binding of three different tetrasaccharide species containing 0, 1 and 2 free amino groups to the NK1 truncated variant of hepatocyte growth factor/scatter factor.


PubMed | Fu Zhou University
Type: Journal Article | Journal: Glycoconjugate journal | Year: 2015

The rare N-unsubstituted glucosamine (GlcNH3(+)) residues in heparan sulfate (HS) have important biological and pathophysiological roles. Therefore, the ability to chemically generate a series of oligosaccharides, which have a similar structure to the naturally-occurring, GlcNH3(+)--containing oligosaccharides from HS, would greatly contribute to investigating their natural role in HS. In this study, a hexasaccharide library that possess GlcNH3(+) residues were prepared from the chemical modification of the fully sulfated dp6. Chemical reaction conditions were optimized to generate different pattern of GlcNH3(+)--containing oligosaccharides, then the structure of the library was detected by high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC/MS-ITTOF) analysis. EIC/MS and MS(2) analysis showed different fragmentation patterns of dp6s with different GlcNH3(+) residues. This provides a foundation for further identification and quantification of GlcNH3(+)--oligosaccharides by mass spectrum analysis.


PubMed | Fu Zhou University
Type: Journal Article | Journal: Glycobiology | Year: 2015

The rare N-unsubstituted glucosamine (GlcNH(3)(+)) residues in heparan sulfate (HS) have important biological and pathophysiological roles. Because of their low natural abundance, the use of chemically generated, structurally defined, N-unsubstituted heparin/HS oligosaccharides can greatly contribute to the investigation of their natural role in HS. However, the sequencing of mixtures of chemically generated oligosaccharides presents major challenges due to the difficulties in separating isomers and the available detection methods. In this study, we developed and validated a simple and sensitive method for the sequence analysis of N-unsubstituted heparin/HS oligosaccharides. This protocol involves pH 4 nitrous acid (HNO(2)) degradation, size-exclusion HPLC and ion-pair reversed-phase liquid chromatography-ion trap/time-of-flight mass spectrometry (IPRP-LC-ITTOF MS). We unexpectedly found that absorbance at 232 nm (normally used for specific detection of C4-C5 unsaturated oligosaccharides) was, in most cases, still sufficiently sensitive to also simultaneously detect saturated oligosaccharides during HPLC, thus simplifying the positional analysis of GlcNH(3)(+)) residues. The IPRP-LC-ITTOF MS system can supply further structural information leading to full sequence determination of the original oligosaccharide. This new methodology has been used to separate and sequence a variety of chemically generated, N-unsubstituted dp6 species containing between 1 and 3 GlcNH(3)(+)) residues per oligosaccharide in different positional combinations. This strategy offers possibilities for the sequencing of natural N-unsubstituted oligosaccharides from HS and should also be applicable, with minor modification, for sequencing at N-sulfated residues using alternative pH 1.5 HNO(2) scission.

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