Fu Yang Teachers College

Fuyang, China

Fu Yang Teachers College

Fuyang, China

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Cai J.,Fu Yang Teachers College | Cai J.,South China Agricultural University | Dai Z.J.,South China Agricultural University | Zhu H.T.,South China Agricultural University | And 4 more authors.
Biologia Plantarum | Year: 2014

Rice chromosome single segment substitution line (SSSL) W23-19-06-06-11 with the genotype Rf3Rf3/Rf4Rf4, a strong restorer line for wild-abortive (WA) cytoplasmic male sterility (CMS), was recently identified from the SSSL library. To investigate the genetic mode of Rf genes and the genetic relationship among WA, yegong (Y), and dwarf-wildabortive (DA) CMS systems, the plants derived from three BC3F2 populations involving W23-19-06-06-11 and the three CMS lines, that carried the Rf3Rf3/Rf4Rf4, Rf3Rf3/rf4rf4, and rf3rf3/Rf4Rf4 genotypes and WA-, Y-, and DA-CMS cytoplasm, were selected and their pollen and spikelet fertility were evaluated. The results show that the genetic effect displayed a trend of Y-CMS > WA-CMS > DA-CMS in the genetic background of W23-19-06-06-11, the effect of Rf4 appeared to be slightly larger than that of Rf3, and their effects were additive for the three CMS systems. Two pairs of dominant genes governed the fertility restoration in pollen and spikelet in the W23-19-06-06-11 which indicates that the genetic mode of the Rf genes was a qualitative character for the three CMS systems. © 2014 Springer Science+Business Media Dordrecht.


Cai J.,Fu Yang Teachers College | Cai J.,South China Agricultural University | Zhang G.,Fu Yang Teachers College
Turkish Journal of Agriculture and Forestry | Year: 2014

The present study was carried out with the objective of developing new parental lines of hybrid rice based on chromosome single-segment substitution lines (SSSLs), on which the restorer and maintainer genes Rf3 (rf3) and Rf4 (rf4) were harbored in rice. To develop the new 3-line hybrid, the SSSL W23-19-06-06-11 with the genotype Rf3Rf3/Rf4Rf4 [a strong restorer line for wild abortive (WA), dwarf-wild abortive (DA), and Yegong (Y) cytoplasmic male sterile (CMS) lines] was crossed 4 times as the male parent with 4 CMS lines: 2 typical WA-type CMS lines of Bobai A and Zhenshan 97A, 1 typical Y-type CMS line of Y-Huanong A, and 1 typical DA-type CMS line of Xieqingzao A. In the BC3F2 populations, the male sterile plants (MSPs) with WA-, Y-, and DA-type CMS sources and nuclear background of W23-19-06-06-11 and their maintainer plants (MPs) were developed by backcrossing and marker-assisted selection. All the MSPs showed 100% pollen and spikelet sterility and the panicle length ranged from 20 to 22.6 cm. The extent of incomplete panicle exertion in the entries ranged from-40.7 to-48.5. The pollen grain viability assay with I2KI of the MSPs revealed 100% pollen sterility, consisting of both typical and spherical abortive pollen grains. The MPs showed normal pollen (spikelet) fertility. A genome-wide DNA marker survey revealed that the numbers of the substituted chromosome segments carried by the MSPs and MPs were 0.8 and 0.6, while the lengths of the substituted chromosome segments corresponding to the Rf3 (or Rf4) locus were, on average, 26.2 cM and 17.0 cM (or 15.3 cM and 15.1 cM). © TÜBİTAK.


Cai J.,South China Agricultural University | Cai J.,Fu Yang Teachers College | Liao Q.P.,South China Agricultural University | Dai Z.J.,South China Agricultural University | And 4 more authors.
Biologia Plantarum | Year: 2013

To reveal the allelic differentiations at the two genes for fertility restoration (Rf) on chromosomes 1 (Rf3) and 10 (Rf4), 15 chromosome single segment substitution lines (SSSLs) with the Rf3 locus and 18 SSSLs with the Rf4 locus were crossed with Bobai A (BbA), a cytoplasmic male sterility line with wild abortive type of cytoplasm (WA-CMS), respectively. Based on the pollen and seed fertility of the F1 hybrids, the Rf3 and Rf4 genes were each classified into four alleles, namely Rf3-1, Rf3-2, Rf3-3, and Rf3-4 for Rf3, and Rf4-1, Rf4-2, Rf4-3, and Rf4-4 for Rf4. Out of the 33 SSSLs, an SSSL W23-19-06-06-11 carrying the genotype Rf3-4Rf3-4/Rf4-4Rf4-4 possessed the strongest restoring ability for BbA. To determine the genetic effects of Rf3 and Rf4 for WA-CMS, one BC3F2 population possessing the genetic background of W23-19-06-06-11 was generated from the cross between W23-19-06-06-11 and BbA by backcrossing and marker-assisted selection. In the BC3F2 population, the plants carrying the Rf3Rf3/Rf4Rf4, Rf3Rf3/rf4rf4, and rf3rf3/Rf4Rf4 genotypes were selected and their phenotyping for pollen and spikelet fertility were evaluated. The result showed that under the genetic background of SSSL W23-19-06-06-11, the effect of Rf4 appeared to be slightly larger than that of Rf3 and their effects were additive for WA-CMS system. These studies will lead to the transfer of Rf genes into adapted cultivars through marker-assisted selection in active hybrid rice breeding programs. © 2013 Springer Science+Business Media Dordrecht.


Wang F.,Fu Yang Teachers College
ICENT 2010 - 2010 International Conference on Educational and Network Technology | Year: 2010

For the problem that the remaining fingerprint cannot be segmented easily, this paper proposes a segmentation algorithm for fingerprint images with remaining ridges based on convex hull model. The algorithm firstly pre-processes fingerprint images,and then discrete points information of fingerprint ridge edge is extracted based on edge detection,subquently the center point as the seed point detects the potential fingerprint contour and using an improved method revised contour so as to obtain its convex hull. Finally we remove the remaining fingerprint based on connected region area calculation. The experimental results show that this method has high accuracy and noise immunity, And it can effectively segment the remaining fingerprint from the foreground. © 2010 IEEE.

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