FSBI Institute of general pathology and pathophysiology RAMS

Moscow, Russia

FSBI Institute of general pathology and pathophysiology RAMS

Moscow, Russia
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Ivanov A.V.,FSBI Institute of general pathology and pathophysiology RAMS | Luzyanin B.P.,FSBI Institute of general pathology and pathophysiology RAMS | Virus E.D.,FSBI Institute of general pathology and pathophysiology RAMS | Rotkina A.S.,FSBI Institute of general pathology and pathophysiology RAMS | Kubatiev A.A.,FSBI Institute of general pathology and pathophysiology RAMS
Electrophoresis | Year: 2014

This work proposes an approach to the direct analysis of S-adenosylhomocysteine (SAH) and the methylation index in blood using CE with UV detection (CE-UV). After application of meglumine postinjection, we achieved SAH in-capillary preconcentration in the HClO4 extracts of erythrocytes, which improved the detection limit (S/N = 3) of SAH up to 3 fmol or 180 nmol/L at the injection volume of 50 nL, taking into account the sample dilution rate. CE-UV was carried out in 30 mM glycine and 45 mmol/L HCl (pH ∼1.8) at 17 kV in a capillary 48 cm in length and 50 μm id. Accuracy of the technique was 101% and reproducibility was about 12%. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim .

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