Time filter

Source Type

Cluj-Napoca, Romania

Clapa D.,Fruit Research Station Cluj | Clapa D.,Babes - Bolyai University | Fira A.,Babes - Bolyai University | Joshee N.,Fort Valley State University
HortScience | Year: 2013

This article presents a new acclimatization method, ex vitro acclimatization in float hydroculture. This protocol ensures the ex vitro acclimatization of the plantlets obtained in vitro in the rooting stage or the ex vitro rooting and acclimatization of the shoots obtained in the multiplication stage. Our hydroculture method is different from the techniques of flotation hydroculture because no fertilizers or plant growth regulators or other biostimulators are used and water oxygenation by bubbling is not provided. Ex vitro rooting and acclimatization in one stage without an in vitro rooting stage in floating cell trays was successfully carried out in Rubus fruticosus and Rosa hybrida cultivars, whereas ex vitro acclimatization of in vitro-rooted plantlets was successfully carried out in Rubus idaeus, Ribes nigrum, Prunus cerasus × P. canescens, Lycium barbarum (Goji berry), Amelanchier canadensis, Drosera rotundifolia, Drosera capillaris, and Nephrolepis sp. In another set of experiments, the floating cell trays were replaced with layers of floating perlite and the latter method was also tested for non-rooted shoots obtained in vitro in the multiplication stage or rooted plantlets obtained in the rooting stage. Direct ex vitro rooting in floating perlite was successful in 'Tayberry' (Rubus fruticosus × Rubus idaeus) (78.12% rooting); Amelanchier canadensis, Rubus fruticosus 'Chester', Rubus idaeus 'Erntesegen', Vaccinium macrocarpon, and Vaccinium corymbosum with rooting percentages above 80%; and Rubus idaeus 'Willamette' and Rosa hybrida 'Cristiana' with rooting percentages above 80%. Source

Clapa D.,Fruit Research Station Cluj | Fira Al.,Fruit Research Station Cluj | Harsan E.,Fruit Research Station Cluj | Chis L.,Fruit Research Station Cluj | And 2 more authors.
Acta Horticulturae | Year: 2010

Californian redwood (Sequoia sempervirens) and giant sequoia (Sequoiadendron giganteum), the two giants of the plant kingdom, grow in the forests of Sierra Nevada in California, but they can also be found in reservations or as natural monuments in various geographical regions. In Transylvania - Romania there are a few specimens of Sequoiadendron giganteum in the Botanical Garden in Cluj-Napoca and in the locality Rogojel - Cluj county, Ardusat - Maramures county, Oradea - Bihor county, Maderat - Arad, Baile Herculane - Caras Severin. Having in view the small number of specimens existing in this region as well as the difficulties regarding the propagation of this species, at the Fruit Research Station at Cluj - Romania, the in vitro propagation of S. sempervirens was studied. This paper presents the history of these specimens in Transylvania as well as aspects regarding the micropropagation of sequoia, such as: the number of shoots/vessel, the number of inoculi obtained/vessel and the multiplication rate on different variants of MS medium by using Plant Agar or Gelrite as gelling agents as well as liquid media with perlite as support matrix. Growth regulators, coconut water (100 ml/L, 200 ml/L and 400 ml/L), kinetin (1, 2, 5 and 10 mg/L) and benzyladenine (0.5 mg/L and 1 mg/L), as well as BAP (3 mg/L) and kinetin (0.2 mg/L) were used. Regarding multiplication rate, the best results were yielded by MS with 2 mg/L kinetin and hormone-free MS with Gelrite as gelling agent. Source

Discover hidden collaborations