Fruit Research Division National Institute of Horticultural and Herbal Science

Suigen, South Korea

Fruit Research Division National Institute of Horticultural and Herbal Science

Suigen, South Korea
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Kim S.-J.,Fruit Research Division National Institute of Horticultural and Herbal Science | Jeong S.-H.,Fruit Research Division National Institute of Horticultural and Herbal Science | Hur Y.-Y.,Fruit Research Division National Institute of Horticultural and Herbal Science | Jung S.-M.,Fruit Research Division National Institute of Horticultural and Herbal Science
Plant OMICS | Year: 2015

Infected 'Campbell Early' grape leaf tissues were collected according to four different tissue locations of brown spot disease: center of the lesion (A), lesion border (C) the uninfected leaf tissue (D) and tissue from A and C as well as the lesion halo (B). A total of 118 metabolites were identified using polar phase GC-MS analysis with sample derivatization and annotated four different sampling tissues. Metabolites were clustered into three groups according to tissues (tissue A, tissue B, and tissues C and D) in the PCA score plot. Tissues C and D were separated in the PCA plot; therefore, they had similar metabolite contents variations. 20 of the metabolites were significantly related to tissues A and C in the S-plot of OPLS-DA. Most of the metabolites, including caffeic acid, succinic acid, and citric acid, were increased in related contents in uninfected tissues (C and D) compared with lesions (A and B). In contrast, D-mannitol, xylitol, and raffinose were increased in the lesion tissues (A and B). Neohesperidin, lactulose, and dehydroascorbic acid were found in the tissue B. As a result, those three metabolites can be expected to relate to the defense mechanism of grapes against brown leaf spot disease.


Chang E.-H.,Fruit Research Division National Institute of Horticultural and Herbal Science | Jung S.-M.,Fruit Research Division National Institute of Horticultural and Herbal Science | Park S.-J.,Extension Service Bureau | Noh J.-H.,Fruit Research Division National Institute of Horticultural and Herbal Science | And 3 more authors.
Plant OMICS | Year: 2014

Harvest time is major factor of affecting fruit and wine quality of grape. But quantify evaluation of wine quality according to harvest time is too difficult. We conducted this study to identify the metabolites, affecting the sensory, quality and fruit characters of grapevine cv. 'Cheongsoo' at the different harvest times, using proton spectroscopy. Our results showed that there was a clear separation of the spectrum data on the PCA plot at each harvest time. In addition, the S-plot of OPLS-DA was useful to identify the essential metabolites across the harvest times. Tartaric acid (δ4.47) was a main factor responsible for the titratable acidity and the acidity of the wine on the sensory test. In addition, both proline (δ2.37) and arginine (δ3.25) are nitrogen source during wine fermentation, and they were closely associated with the body and balance of the wine on the sensory test. The relative concentration of proline was increased across the harvest times, and it was a positive factor that is associated with changes in the body and balance of the wine. In conclusion, our results indicate that the metabolic profiling based on NMR spectroscopy, combined with a multivariate analysis, is useful in identifying metabolites that are responsible for quality of grape and wine.

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