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Meng Q.,Ocean University of China | Meng Q.,Freshwater Fisheries Research Institute of Shandong Province | Bao Z.,Ocean University of China | Wang Z.,Ocean University of China | And 4 more authors.
Journal of Shellfish Research | Year: 2012

The successful induction of triploid embryos or larvae has been performed in Patinopecten yessoensis during the past 2 decades. However, no research has been reported about the performance of triploid P. yessoensis cultured in the field. This study induced triploidy in P. yessoensis by hypotonic shock and compared the growth and reproductive performance of triploids and diploids reared under commercial conditions for up to 24 mo. The main results of this study are as follows: Triploid scallops were smaller in size and weight compared with diploids and had a retarded absolute growth rate (AGR). After 24 mo of cultivation, the mean shell height, shell length, shell width, and body weight of triploids were 9%, 10%, 9%, and 25% less than diploids, respectively (P < 0.01). Although normal in sex ratio, the reproductive potential of triploids was significantly reduced. Only 87% of the triploids exhibited sex-discernible gonads during the breeding season. None of the male triploids spawned, and the percentage of female spawners among the triploid population was only 27% of that for the diploid population. The relative fecundity of triploid females was only 4% of diploid females. Triploid eggs produced mostly aneuploid larvae and had an extremely small chance of generating viable offspring when fertilized by sperm from diploid males. Most aneuploid larvae died before the D-shaped stage, and no survival exceeded 7 days. The potential to yield viable offspring from the triploid population was estimated to be only 4% × 10-6 of that of the diploid population. Despite the growth disadvantage of triploids, this study may support, in part, the energy reallocation hypothesis, because triploid AGR was similar to diploid AGR (2% variance) during the sexual maturation season. Our results also indicate that there would be no growth advantage, but instead a disadvantage, for triploid P. yessoensis growing at the experiment site. In addition, this research provides the first evidence that viable triploid molluscs can be induced by hypotonic shock, of which the practical and evolutionary implications are also discussed. Copyright © 2013 BioOne.

Song L.P.,Freshwater Fisheries Research Institute of Shandong Province | Han B.,Shanghai Ocean University | Wang A.Y.,Freshwater Fisheries Research Institute of Shandong Province | Hu B.,Freshwater Fisheries Research Institute of Shandong Province | Mao S.Q.,Freshwater Fisheries Research Institute of Shandong Province
Israeli Journal of Aquaculture - Bamidgeh | Year: 2010

The effects of dietary lipid on growth and feed utilization of sooty grunter juveniles (Hephaestus fuliginosus) were examined. Triplicate groups of 20 juveniles were fed diets containing 39% crude protein, supplemented with 0%, 3%, 6%, 9%, or 12% lipid (% dry matter) for 60 days. At the end of the experiment, at least 90% survived in all diet groups. Fish fed the diet containing 6% lipids had a significantly (p<0.05) higher growth rate and better feed conversion and protein efficiency ratios than the other groups. Muscle lipid contents increased dramatically as the dietary lipid level increased and the highest lipid content was obtained in fish fed the highest lipid diet. On the other hand, muscle protein contents declined as the dietary lipid level increased, and the lowest protein value was in fish fed the 12% lipid diet. Ash and dry matter contents did not significantly differ among groups. Increasing the lipid level to 6% was the most effective for improving fish growth and feed efficiency.

Meng Q.,Ocean University of China | Meng Q.,Freshwater Fisheries Research Institute of Shandong Province | Wang S.,Ocean University of China | Zhang L.,Ocean University of China | And 2 more authors.
Chinese Journal of Oceanology and Limnology | Year: 2013

In situ loop-mediated isothermal amplification (in situ LAMP) combines in situ hybridization and loop-mediated isothermal amplification (LAMP) techniques for chromosomal localization of DNA sequences. In situ LAMP is a method that is generally more specific and sensitive than conventional techniques such as fluorescence in situ hybridization (FISH), primed in situ labeling (PRINS), and cycling primed in situ labeling (C-PRINS). Here, we describe the development and application of in situ LAMP to identify the chromosomal localization of DNA sequences. To benchmark this technique, we successfully applied this technique to localize the major ribosomal RNA gene on the chromosomes of the Zhikong scallop (Chlamys farreri). © 2013 Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg.

Liu F.,Freshwater Fisheries Research Institute of Shandong Province | Liu F.,Ocean University of China | Li X.-Q.,Freshwater Fisheries Research Institute of Shandong Province | Dong G.-C.,Freshwater Fisheries Research Institute of Shandong Province | And 4 more authors.
Zhongguo Huanjing Kexue/China Environmental Science | Year: 2011

14 representative water-sample sites were selected around the coastal wetland of Yellow River Estuary and three times surveys were carried out on May (dry period), August (wet period) and November (level period), respectively. 22 pollution indexes such as nutritive salt, heavy metal, petroleum etc. were measured. The pollution status was assessed with Nemero Index and comprehensive nutritional status index (TLI). The results showed that the water quality of coastal wetland was light eutrophication and deteriorated with each passing year. The slight pollution sites were found at wetland lakes, Yellow River old course, the national nature reserve, culture ponds and other static state water area, whereas the tributaries like Guangli River, Tiao River, Shenxiangou River, Xiaodao River and Gudong oil fields drainage ditch were serious polluted which were more serious polluted than the Yellow River. TN, TP, NH4 +-N, UIA, Chl-a and petroleum were main pollutants at coastal wetland. As for the pollution of nitrogen and phosphorus, comparing with historical data, deteriorated significantly. The whole water quality was not optimistic in Yellow River estuary, although no significantly increase of petroleum pollutants and Hg pollution and no detection of other heavy metals.

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