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Han Y.,Kagoshima University | Han R.,Freshwater Fisheries Research Academy of Liaoning Province | Koshio S.,Kagoshima University | Ishikawa M.,Kagoshima University | And 2 more authors.
Aquaculture | Year: 2014

This study was carried out to investigate the interactive effects of dietary leucine and valine on the performances of two sizes Japanese flounder Paralichthys olivaceus. Two groups of fish with average initial weights of 0.43. ±. 0.01. g and 41.2. ±. 2.0. g (mean. ±. S.D.) were fed with diets containing two levels of leucine (1.6 and 5.0% of diet) with three levels of valine (1.2, 1.8 and 2.5% of diet, as a 2. ×. 3 experimental design) and the control diet for 56. days and 30. days, respectively. In trial 1, after the fish fed with test diets for 56. days, growth performance and nutrients utilization were evaluated. Interactive effects of leucine and valine were found on growth parameters (final body weight, body weight gain and special growth rate) of Japanese flounder. Not only antagonism was observed in high dietary Leu level groups but also the stimulative effect of increased dietary Val in low Leu level groups. Significant interaction was also found on feed conversion ratio of Japanese flounder fed with test diets, however, the fish whole body proximate compositions were not altered by the various diets. In trial 2, dietary Leu and Val dominated the plasma free Leu and free Val concentrations, but no antagonism was found. Interactions were also found on plasma LDH and GPT of test fish, and it was showed that for fish fed with high dietary Val level in low Leu (1.6% of diet) diet can protect the cells from oxidative stress. © 2014 Elsevier B.V.

Yang P.-M.,Freshwater Fisheries Research Academy of Liaoning Province | Jin G.-H.,Freshwater Fisheries Research Academy of Liaoning Province | Liu Y.-X.,Freshwater Fisheries Research Academy of Liaoning Province | Li J.-W.,Freshwater Fisheries Research Academy of Liaoning Province | Hu Z.-Y.,Freshwater Fisheries Research Academy of Liaoning Province
Iranian Journal of Fisheries Sciences | Year: 2012

To investigate the ontogeny of Perccottus glenii, embryonic, larval and juvenile development of P. glenii were examined under captive condition. The fertilized eggs with numerous oil droplets were orange-pink in color, prolate spherical in shape and had average length of 3.32±0.14 mm and width of 1.24±0.04 mm. From fertilization to hatching, the ontogenesis of the fish spent more than 200 h, and the process of embryonic development was divided into 25 stages based on the morphological characteristics. The newly hatched larvae, with well-developed swim bladder and pectoral fins, measured 5.07±0.18mm in total length. Initial feeding occurred at day 2 after hatching (AH) and the complete absorption of yolk sphere was observed 3 days after hatching. At day 40 AH, scales and vertical pigment were found to be appear. Scales covered the entire body and all fins were well developed 70 days AH, at which time the juveniles reached the young stage with a total length (TL) of 24.74±4.28 mm. At day 140 AH, The fry with a TL of 29.57±4.65 mm, were morphologically similar to the adults except for size.

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