Freshwater Aquaculture Collaborative Innovation Center

Wuhan, China

Freshwater Aquaculture Collaborative Innovation Center

Wuhan, China
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Kong W.-G.,Huazhong Agricultural University | Kong W.-G.,Key Laboratory of Freshwater Animal Breeding | Kong W.-G.,Freshwater Aquaculture Collaborative Innovation Center | Li S.-S.,Huazhong Agricultural University | And 14 more authors.
Fish Physiology and Biochemistry | Year: 2017

The aim of this study is to explore the effect of Aeromonas hydrophila on the intestinal mucosal barrier structure and intestinal permeability in grass carp (Ctenopharyngodon idella). Histopathological examinations showed that A. hydrophila induced severe intestinal lesions, including inflammatory cell infiltration and intestinal villus fusion and swelling. Messenger RNA (mRNA) expression of the inflammatory cytokines TNF-α, IL-1β, IL-8, IL-10 and MyD88 was significantly increased after infection with A. hydrophila. The permeability of intestinal mucosa was determined using Evans blue (EB) and d-lactic acid. The results indicated that the levels of EB and serum d-lactic acid were significantly increased after infection with A. hydrophila (p < 0.05). Our results also indicated that the intestinal mucosal barrier injury induced by A. hydrophila infection was closely associated with the expression of the tight junction (TJ) protein zonula occludens-1 (ZO-1), occludin, claudin b and claudin c as well as the activity of Na+, K+-ATPase and Ca2+, Mg2+-ATPase. Lower mRNA levels of occludin and lower Na+, K+-ATPase and Ca2+, Mg2+-ATPase activity in the intestines were observed after challenge. ZO-1 and claudin c were significantly increased 24 h after infection with A. hydrophila. The most interesting finding was that claudin b also significantly increased 24 h after challenge and then decreased to lower levels at 72, 120 and 168 h post-infection compared to the PBS-treated control group. The results demonstrated that grass carp infection with A. hydrophila induced intestinal inflammation and impaired the structure and function of the intestinal mucosal barrier. © 2017 Springer Science+Business Media Dordrecht


Ji W.,Huazhong Agricultural University | Ji W.,Freshwater Aquaculture Collaborative Innovation Center | Zhang G.-R.,Huazhong Agricultural University | Zhang G.-R.,Freshwater Aquaculture Collaborative Innovation Center | And 7 more authors.
Mitochondrial DNA | Year: 2015

The complete mitochondrial genome of Oxygymnocypris stewartii is 16,646 bp in size, containing 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and 2 non-coding regions (the control region and the origin of light-strand replication). The gene order of O. stewartii mitogenome is similar to those observed in most other vertebrates. The complete mitogenome sequence information of O. stewartii can provide useful data for further studies on phylogenetics, stock evaluation and conservation genetics. © 2013 Informa UK Ltd.


Zhang G.-R.,Huazhong Agricultural University | Zhang G.-R.,Freshwater Aquaculture Collaborative Innovation Center | Ji W.,Huazhong Agricultural University | Ji W.,Freshwater Aquaculture Collaborative Innovation Center | And 7 more authors.
Mitochondrial DNA | Year: 2015

The complete mitochondrial genome of Ptychobarbus dipogon is 16,787 bp in length, consisting of 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and 2 non-coding regions: control region (CR) and origin of light-strand replication (OL). The gene order of P. dipogon mitogenome is similar to those observed in most other vertebrates. The complete mitogenome sequence of P. dipogon can provide useful data for further studies on population structure, phylogenetics and conservation genetics of this species. © 2013 © 2013 Informa UK Ltd.


Xu H.,Huazhong Agricultural University | Xu H.,Freshwater Aquaculture Collaborative innovation Center | Ma Z.,Huazhong Agricultural University | Ma Z.,Freshwater Aquaculture Collaborative innovation Center | And 6 more authors.
Mitochondrial DNA | Year: 2016

In this study, we cloned and sequenced the complete mitochondrial genome of Macropodus ocellatus. The genome is a circular molecule of 16,501 bp in length and consists of 13 proteincoding genes, 22 tRNA genes, 2 ribosomal RNA genes and 2 main non-coding regions (the control region and the origin of the light strand replication). The gene composition and order of which were similar to most other vertebrates. All protein-coding genes are initiated with ATG except for COX 1, which begin with GTG instead. However, the termination codons of 13 protein-coding genes are varied with TAA, TA, T or AGG. This mitogenome sequenc data would contribute to better understanding species identification, evolutionary relationships and population genetics of the Macropodus. © 2014 Informa UK Ltd.

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