Fred Hutchinson Cancer Research Center
Fred Hutchinson Cancer Research Center
The Fred Hutchinson Cancer Research Center , is one of the world’s leading cancer research institutes. Its interdisciplinary scientists research the prevention, early detection, and treatment of cancer and other diseases in the laboratory and at patient bedside in the United States and other countries.Fred Hutch's mission statement is "the elimination of cancer and related diseases as causes of human suffering and death". Wikipedia.
Fred Hutchinson Cancer Research Center | Date: 2017-04-12
The present disclosure provides methods for immortalizing precursor cells that are non-terminally differentiated cells such as stem cells, the methods comprising culturing the precursor cells in the presence of a Notch 1 agonist, Notch 2 agonist or Notch 1 agonist and Notch 2 agonist (and, in particular embodiments, one or more growth factors) that support the proliferation but not differentiation of the non-terminally differentiated cells. The present disclosure further provides methods to induce the differentiation of immortalized cells, comprising growing the cells in the presence of a Notch 1 agonist, Notch 2 agonist or Notch 1 agonist and Notch 2 agonist and at least one growth factor which supports the differentiation of the cell into a more specialized cell type. The immortalized and/or differentiated cells of the disclosure can be used to repopulate cell populations that have been diminished, for example as a result of infection or exposure to certain drugs. The disclosure further provides a cell culture comprising a population of non-terminally differentiated cells immortalized by the methods of the present disclosure and kits comprising reagents that promote the immortalization of precursor cells.
Adaptive Biotechnologies Corporation and Fred Hutchinson Cancer Research Center | Date: 2017-02-22
A relative representation of adaptive immune cells in a biological sample is quantified using multiplex PCR and sequencing of adaptive immune cells, control genes, and synthetic template molecules. Disclosed herein are methods for quantifying a number of adaptive immune cells in a biological sample, and methods for quantifying a relative representation of adaptive immune cells in a biological sample that comprises a mixture of cells comprising adaptive immune cells and cells that are not adaptive immune cells. Methods are provided for amplifying by multiplex PCR and sequencing a first set of synthetic templates each comprising one TCR or IgV segment and one TCR of Ig J or C segment and a unique bar code.
Fred Hutchinson Cancer Research Center | Date: 2017-07-05
The present invention relates to methods of treating immune disorders and cancers. In particular the invention provides methods of inhibiting the negative immunomodulatory effects of ERp-5 on T cells and dendritic cells, in conjunction with other surface receptors.
Fred Hutchinson Cancer Research Center, University of Rochester and Leiden University | Date: 2017-01-25
Embodiments provide methods and compositions related to determining or predicting the presence or risk of developing DUX-4-related diseases such as muscular dystrophy. In particular, methods and compositions may be provided by identifying patients with Facioscapulohumeral muscular dystrophy (FSHD). Methods and compositions are based, in part, on the discovery that expression of certain biomarkers in patients or subjects can determine the presence or risk of developing DUX-4-related diseases such as muscular dystrophy, or particularly FSHD. Further disclosed are DUX-4 biomarkers that can be used for the methods.
Fred Hutchinson Cancer Research Center | Date: 2017-06-14
The present disclosure provides high affinity and enhanced affinity T cell receptors specific for human Wilms tumor protein 1 (WT 1) epitopes for use in treating diseases or disorders, such as cancer cells that overexpress WT-1.
Fred Hutchinson Cancer Research Center | Date: 2017-05-10
Chlorotoxin variants, chlorotoxin variant conjugates, compositions that include the chlorotoxin variants or conjugates, and methods for using the chlorotoxin variants, conjugates, and compositions. In particular, conjugates comprising a chlorotoxin variant where lysine K15 and/or lysine K23 are substituted by other amino acids, coupled to a fluorescent label.
Fred Hutchinson Cancer Research Center and Board Of Regents Of The University Of Texas System | Date: 2017-03-29
The current disclosure describes methods of expanding precursor cells for hematopoietic transplantation in subjects. The methods culture precursor cells in media containing an immobilized high molecular weight LILRB2 agonist or an LILRB2 agonist in combination with a Notch agonist. The expanded cells can be used to treat a variety of hematopoietic disorders.
Fred Hutchinson Cancer Research Center | Date: 2017-01-06
The present disclosure provides compositions, kits, and methods to protect organs by inducing acquired cytoresistance without causing injury to the organ. The compositions, kits, and methods utilize heme proteins, iron and/or vitamin B12 and, optionally, agents that impact heme protein metabolism.
Houghton A.M.,Fred Hutchinson Cancer Research Center
Nature Reviews Cancer | Year: 2013
Numerous epidemiological studies have consistently linked the presence of chronic obstructive pulmonary disease (COPD) to the development of lung cancer, independently of cigarette smoking dosage. The mechanistic explanation for this remains poorly understood. Progress towards uncovering this link has been hampered by the heterogeneous nature of the two disorders: each is characterized by multiple sub-phenotypes of disease. In this Review, I discuss the nature of the link between the two diseases and consider specific mechanisms that operate in both COPD and lung cancer, some of which might represent either chemopreventive or chemotherapeutic targets. © 2013 Macmillan Publishers Limited. All rights reserved.
Maloney D.G.,Fred Hutchinson Cancer Research Center
New England Journal of Medicine | Year: 2012
A 62-year-old man is evaluated for abdominal pain. Computed tomography (CT) shows a 7-cm mesenteric mass. An incisional biopsy reveals a diffuse large B-cell lymphoma, and immunohistochemical staining is positive for the B-cell antigen CD20. The patient is referred to an oncologist. A positron-emission tomographic-CT (PET-CT) scan reveals involvement of additional nodes in the lower chest and abdomen. The serum level of lactate dehydrogenase is twice the upper limit of the normal range. The results of bone marrow biopsy and aspiration are normal on pathological analysis and flow cytometry. The patient reports an unintentional weight loss of 111/2 kg (25 lb) during the previous 6 months but no unexplained fevers or night sweats. He has no history of cardiac disease, and an echocardiogram shows a normal left ventricular ejection fraction. The oncologist recommends treatment with the anti-CD20 monoclonal antibody rituximab in combination with a chemotherapy regimen of cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP). Copyright © 2012 Massachusetts Medical Society.