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Liu L.,Liaoning Medical University | Jiao J.,Shenyang Medical College | Wang Y.,Liaoning Medical University | Zhang D.,Fourth Peoples Hospital of Shenyang | And 2 more authors.
PLoS ONE | Year: 2014

Objective: The TP53BP1 gene may be involved in the development of cancer through disrupting DNA repair. However, studies investigating the relationship between TP53BP1 Glu353Asp (rs560191) polymorphism and cancer yielded contradictory and inconclusive outcomes. In order to realize these ambiguous findings, a meta-analysis was performed to assess the association between the TP53BP1 Glu353Asp (rs560191) polymorphism and susceptibility to cancer. Methods: We conducted a search of all English reports on studies for the association between the TP53BP1 Asp353Glu (rs560191) polymorphism and susceptibility to cancer using Medline, the Cochrane Library, EMbase, Web of Science, Google (scholar), and all Chinese reports were identified manually and on-line using CBMDisc, Chongqing VIP database, and CNKI database. The strict selection criteria and exclusion criteria were determined, and odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the strength of associations. The fixed or random effect model was selected based on the heterogeneity test among studies. Publication bias was estimated using funnel plots and Egger's regression test. Results: A total of seven studies were included in the meta-analysis including 3,213 cases and 3,849 controls. The results indicated that the Glu353Asp (rs560191) polymorphism in TP53BP1 gene had no association with cancer risk for all genetic models. In the subgroup analysis, the results suggested that Glu353Asp polymorphism was not associated with the risk of cancer according to ethnicity, cancer type, genotyping method, adjusted with control or not, HWE and quality score. Conclusions: This meta-analysis suggested that the Glu353Asp (rs560191) polymorphism in TP53BP1 gene was not associated with risk of cancer. Copyright: © 2014 Liu et al. Source

Chen P.,Fourth Peoples Hospital of Shenyang | Wang J.,Liaoning Medical University
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: Lung cancer stem cells are tightly related to the treatment and prognosis of lung cancer. We can provide more references for clinical diagnosis and treatment of lung cancer through the study on the tumorigenicity and surface markers of lung cancer stem cells. OBJECTIVE: To explore the enrichment methods for lung cancer stem cells and cellular tumorigenicity. METHODS: Lung cancer stem cells were induced in serum-free culture medium containing epidermal growth factor, insulin-like growth factor 1, and basic fibroblast growth factor. Then, the expressions of related surface markers were detected using immunofluorescence method. After that, mice were implanted subcutaneous with lung cancer stem cell spheres to understand the tumorigenicity of lung cancer stem cells. RESULTS AND CONCLUSION: Lung cancer stem cells under serum-free induction and culture were changed to sphere-forming cells, and the immunofluorescence detection showed that over 80% of sphere-forming cells were positive for CCSP, SP-C and OCT4. After transplantation of sphere-forming cells, the mice showed a high tumorigenicity. These findings indicate that sphere-forming cells are formed after serum-free suspension culture of lung cancer stem cells, which have a higher tumorigenicity. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved. Source

Chen F.-Q.,Shenyang University | Wang J.,Shenyang Medical College | Liu X.-B.,Shenyang University | Ma X.-Y.,Shenyang University | And 4 more authors.
Journal of Diabetes Research | Year: 2013

Although the pathogenetic mechanism of DN has not been elucidated, an inflammatory mechanism has been suggested as a potential contributor. This study was designed to explore the relationship between low-grade inflammation and renal microangiopathy in T2DM. A total of 261 diabetic subjects were divided into three groups according to UAE: a normal albuminuria group, a microalbuminuria group, and a macroalbuminuria group. A control group was also chosen. Levels of hs-CRP, TNF-α, uMCP-1, SAA, SCr, BUN, serum lipid, blood pressure, and HbA1c were measured in all subjects. Compared with the normal controls, levels of hs-CRP, TNF-α, uMCP-1, and SAA in T2DM patients were significantly higher. They were also elevated in the normal albuminuria group, P<0.05. Compared with the normal albuminuria group, levels of these inflammatory cytokines were significantly higher in the microalbuminuria and macroalbuminuria group, P<0.01. The macroalbuminuria group also showed higher levels than the microalbuminuria group, P<0.01. Also they were positively correlated with UAE, SBP, DBP, LDL-C, and TC. We noted no significance correlated with course, TG, or HDL-C. Only TNF-α; was positively correlated with HbA1c. This study revealed the importance of these inflammatory cytokines in DN pathogenesis. Further studies are needed to fully establish the potential of these cytokines as additional biomarkers for the development of DN. © 2013 Fen-qin Chen et al. Source

Xiao H.,Liaoning Medical University | Fan Z.-Y.,463th Hospital of the Peoples Liberation Army | Tian X.-D.,Fourth Peoples Hospital of Shenyang | Xu Y.-C.,Liaoning Medical University
International Journal of Ophthalmology | Year: 2014

AIM To study the efficacy difference between form-deprived myopia (FDM) and lens-induced myopia (L1M), the degree of myopia, axial length and pathological changes of the posterior sclera from guinea pigs were evaluated. METHODS Four-week pigmented guinea pigs were randomly assigned into 3 groups, including normal control (n=6), FDM group with monocular cover (n=i 1) and LIM group with monocular -7Dlens treatment (n=i 1). FDM group was form-deprived while LIM group was lens-induced for 1 4 d. Refractive error and axial length were measured prior to and post treatment, respectively. Morphological changes of sclera were examined using both light and electronic microscopes. RESULTS After 1 4d treatment, refractive errors for FDM group and LIM group were -3 .05 ±0.7 1 D and -2.1 2 ±1.29 D, respectively, which were significantly more myopic than that of normal controls and fellow control eyes (Po.os). Under light microscope, both FDM group and LIM group showed thinned sclera, disarrangement of fibrosis and enlarged disassociation between fibers. Consistently, ultrastructural examination showed degenerated fibroblasts and thinned fibers in posterior sclera. CONCLUSION Following two weeks of myopia induction in guinea pigs, with regard to the degree of myopia, axial length and pathological alterations, there was no significant difference between FDM and LIM models. Therefore, FDM and LIM are equally effective and useful as a model of experimental myopia and guinea pigs are ideal animals for induction of experimental myopia because their high sensitivity to both form-deprivation and lens-induction. © International Journal of Ophthalmology Press. Source

Sun L.,Shenyang University | Yuan Q.,Shenyang University | Cao N.,General Hospital of Shenyang Military Command | Guo W.,Fourth Peoples Hospital of Shenyang | And 4 more authors.
Scientific World Journal | Year: 2014

Objective. This meta-analysis aimed to investigate a comprehensive and reliable conclusion on the correlations of single nucleotide polymorphisms (SNPs) in the vascular endothelial growth factor (VEGF) gene with the risk of diabetic nephropathy (DN) in patients with diabetes mellitus (DM). Methods. We screened PubMed, Embase, Web of Science, Cochrane Library, CISCOM, CINAHL, Google Scholar, CBM, and CNKI databases for those relevant studies that investigated the association of 14,945 subjects with clinicopathological parameters in gastric cancer. Results. Eleven case-control studies that met all inclusion criteria were included in this meta-analysis. A total of 14,945 subjects were involved, including 3,049 DN patients and 11,896 DM patients. Our meta-analysis results revealed that VEGF rs2010963 and rs3025039 polymorphisms might contribute to the risk of DN in DM patients. Ethnicity-stratified analysis suggested that VEGF genetic polymorphisms were associated with an increased risk of DN among Asians. However, we found no correlations of VEGF genetic polymorphisms with susceptibility to DN among Caucasians. Conclusion. Our findings suggest that VEGF rs2010963 and rs3025039 polymorphisms may contribute to the risk of DN in DM patients, especially among Asians. Thus, VEGF genetic polymorphisms could be useful biomarkers for early diagnosis of DN in DM patients. © 2014 Li Sun et al. Source

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