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Ma C.,Fourth Peoples Hospital of Jinan City | Liu A.,Fourth Peoples Hospital of Jinan City | Li Z.,Fourth Peoples Hospital of Jinan City | Zhou X.,Shandong University of Traditional Chinese Medicine | Zhou S.,Shandong University of Traditional Chinese Medicine
Journal of Clinical Neuroscience | Year: 2014

This study investigated the clinical value of diffusion tensor imaging (DTI) in predicting the motor outcome in patients with basal ganglia hemorrhage. This prospective study included 23 patients assessed with DTI to measure the fractional anisotropy (FA) value in affected cortical spinal tract (CST) at three time points: day 0, day 30 and day 90 after onset. The motor function score (MFS) was applied to evaluate motor function and patients were divided into good and poor outcome groups according to the MFS on day 90. The mean FA value on day 0 was significantly lower in the poor outcome group than in the good outcome group (p < 0.01). FA value gradually decreased in the poor outcome group until day 90 after onset, while it continuously increased in the good outcome group. The MFS obtained at day 90 after onset was significantly correlated with the initial FA value in the affected cerebral peduncle (r = -0.926, p < 0.01). Receiver operating characteristic curve analysis showed that the FA value on day 0 could predict motor function outcome with a sensitivity of 88.89% and specificity of 92.86% at the initial FA value of 0.45. The FA value of affected CST in acute cerebral hemorrhage may valuably predict the motor function outcome and its dynamic change may represent the Wallerian degeneration in motor tracts after hemorrhagic stroke. © 2014 Published by Elsevier Ltd.


Ma C.-C.,Shandong University | Ma C.-C.,Fourth Peoples Hospital of Jinan City | Liu A.-J.,Fourth Peoples Hospital of Jinan City | Liu A.-H.,Fourth Peoples Hospital of Jinan City | And 2 more authors.
Clinical EEG and Neuroscience | Year: 2014

Alzheimer's disease (AD) is the most common cause of dementia. Global field synchronization (GFS) can measure functional synchronization in frequency-domain electroencephalogram (EEG) data. The aim of this study is to explore GFS values and its clinical significance for severity of cognitive decline in AD. EEGs were recorded from 37 AD patients and 37 age-matched healthy individuals. GFS values were calculated in delta, theta, alpha, beta 1, beta 2, beta 3, gamma, and full frequency bands. The Montreal Cognitive Assessment (MoCA) and Clinical Dementia Rating scale (CDR) were employed to assess symptom severity in AD patients. Correlation analysis, clustering analysis, and concordance analysis were performed to analyze the relationship between GFS values and MoCA scores in AD patients. GFS values of the beta 1, beta 2, beta 3, and full bands were lower in AD patients than in healthy individuals, and positively correlated with MoCA and CDR scores in the combined group (AD patients and healthy individuals). GFS values were positively correlated with MoCA socres in 3 beta bands and full bands, and with CDR scores in the delta band. There was a good concordance between K-means clustering algorithm calculating of GFS values and MoCA scoring (=.913, P <.001). In conclusion, the present results indicated that GFS can serve as an indicator of cognitive decline or impairment in AD patients. Furthermore, the GFS method of EEG holds considerable promise to distinguish mild cognitive impairment from serious cognitive impairment in patients with AD. © EEG and Clinical Neuroscience Society (ECNS) 2013.


Dong X.-L.,Xi'an Jiaotong University | Gong Y.,Fourth Peoples Hospital of Jinan City | Chen Z.-Z.,Zibo Central Hospital | Wang Y.-J.,Xi'an Jiaotong University
Chinese Journal of Integrative Medicine | Year: 2014

Objective: To investigate the effect of Delisheng Injection (DLS), a Chinese medicinal compound, DLS combined with cis-platinum (DDP), an active agent used in lung cancer chemotherapy, on a human highly metastatic giant lung carcinoma cell line PGCL3. Methods: The suspended PGCL3 cells at 105 /mL cultured in 96-well tissue culture plates were divided into 4 groups: DLS treatment group (2 μL/mL, 5 μL/mL, 10 μL/mL, 25 μL/mL), DDP treatment group (1 μg/mL, 2 μg/mL, 5 μg/mL, 15 μg/mL), combined DLS with DDP treatment group (DLS:DDP 2 μL/mL:1 μg/mL, 5 μL/mL:2 μg/mL, 10 μL/mL:5 μg/mL, 25 μL/mL:15 μg/mL) and a control group. The cytotoxicity of DLS with different concentrations (2 μL/mL, 5 μL/mL, 10 μL/mL, 25 μL/mL) on PGCL3 cells was determined by 3-(4,5)- dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay. Effect of DLS on adhesion of PGCL-3 cells was tested by cell-matrigel adhesion assay. Chemotactic movement model of transwell camerula was used to determine the effect of DLS on invasion and migration of PGCL-3 cells. Results: Compared with the control group, DLS (2 μL/mL, 5 μL/mL, 10 μL/mL, 25 μL/mL) could significantly decrease cell proliferation, adhesion, invasion and migration abilities (P <0.05). Cell adhesion, invasion and migration abilities were significantly decreased after combination treatment of DLS:DDP (2 μL/mL:1 μg/mL, 5 μL/mL:2 μg/mL, 10 μL/mL:5 μg/mL, 25 μL/mL:15 μg/mL) compared with DDP single-agent treatment (1 μg/mL, 2 μg/mL, 5 μg/mL, 15 μg/mL, P<0.05), respectively. Conclusions: DLS single-agent has a satisfying inhibition effect in PGCL3 cell line and DLS might enhance the inhibition effect of DDP on cancer metastasis. Our research provided a experimental basis about the treatment on highly metastatic lung caner. © 2013 Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag.


Qu Q.,Fourth Peoples Hospital of Jinan City | Dai B.,Fourth Peoples Hospital of Jinan City | Yang B.,Fourth Peoples Hospital of Jinan City | Li X.,Fourth Peoples Hospital of Jinan City | And 2 more authors.
BioMed Research International | Year: 2014

In the present study, we aimed to investigate the preventive effects of 4-hydroxychalcone (4HCH) on resistant hypertension. We used cryptochrome-null mice, which characteristically show high plasma aldosterone levels, inflammation, and renal injury. The cryptochrome-null mice received high-salt treatment and were treated orally with 4HCH 10 mg/kg, 4HCH 20 mg/kg, and 4HCH 40 mg/kg, respectively. The salt administration in cryptochrome-null mice is able to induce an increase in systolic pressure which is associated with hyperaldosteronism, inflammation, and kidney injury. Treatment with 40 mg/kg 4HCH reduced systolic hypertension, serum IL-1β, and TNF-α levels and suppressed the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-B) and renal injury. The impact of 4HCH on the hyperaldosteronism, inflammation, and kidney injury provides new insights for future development of therapeutic strategies in resistant hypertension. © 2014 Qi Qu et al.


Liu B.,Fourth Peoples Hospital Of Jinan City | Wang X.-M.,Fourth Peoples Hospital Of Jinan City | Pan Q.,Fourth Peoples Hospital Of Jinan City | Tian Q.-J.,Fourth Peoples Hospital Of Jinan City
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: It is discovered recently that miRNA is a new regulator that is able to have an impact on gene expression and miRNA contributes to proliferation, differentiation and self-renewal of pluripotent stem cells. OBJECTIVE: To investigate the mechanism by which miR-155 regulates chondrogenic differentiation of bone marrow mesenchymal stem cells. METHODS: Sixty healthy Sprague-Dawley aged 12 weeks were randomized into study group and control group, with 30 in each group. Under anesthesia, rats were sacrificed to harvest bone marrow of the lower limbs. Then bone marrow mesenchymal stem cells were isolated, cultured, and transfected with miR-155 mimics in the study group and a negative control sequence in the control group. After chondrogenic induction, RT-PCR was used to detect the expressions of Sox9, Collagen II, Aggrecan and Collagen X gene, and western blot assay to detect the expression of Sox9 and Runx2 proteins. RESULTS AND CONCLUSION: Compared with the control group, the mRNA expressions of Sox9, Collagen II and Aggrecan were higher, but the mRNA expression of Collagen X was lower in the study group (P < 0.05); the protein expression of Sox9 was higher, but the protein expression of Runx2 was lower in the study group (P < 0.05). These findings indicate that miR-155 promotes the chondrogenic differentiation of bone marrow mesenchymal stem cells and moreover, it can suppress the hypertrophy trend of bone marrow mesenchymal stem cells differentiating into chondrocytes. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.


Li Z.,Fourth Peoples Hospital of Jinan City
The Journal of international medical research | Year: 2012

To examine whether there is an association between the serum concentration of vaspin and the presence of carotid plaque in early stage type 2 diabetes mellitus (T2DM). Patients (n = 61) with T2DM within 3 years of diagnosis were divided into those with and those without carotid plaque. Fasting serum vaspin levels, measured by enzymelinked immunosorbent assay, and blood pressure were compared between these two groups and also with an age-matched, apparently healthy control group (n = 26). Fasting serum vaspin concentrations were significantly higher in patients with T2DM without carotid plaque than in controls, but significantly lower in T2DM patients with carotid plaque than in those without. Multivariate logistic regression analysis showed a significant positive association between the presence of carotid plaque and systolic blood pressure and a significant inverse association between the presence of carotid plaque and fasting serum vaspin concentration. A significant inverse association was found, in patients with T2DM within 3 years of diagnosis, between serum vaspin concentration and the presence of carotid plaque.


Li J.,Fourth Peoples Hospital of Jinan City | Yin L.-L.,Fourth Peoples Hospital of Jinan City | Wang J.,Fourth Peoples Hospital of Jinan City | Zhang G.-F.,Fourth Peoples Hospital of Jinan City
Chinese Journal of Cancer Prevention and Treatment | Year: 2012

OBJECTIVE: To investigate expression of PTEN and of Skp2 in colorectal cancer and aseess their correlation to clinical pathological diameters and prognosis. METHODS: Enhanced immunohistochemical staining (SP) was applied to detect expression of PTEN and of Skp2 in colorectal cancer group and control group. Correlations of expression of PTEN and Skp2 to clinical pathological parameters and prognosis and their correlations were analysed. RESULTS: The positive rate of PTEN in colorectal cancer group (72.13%) were lower than those in control group(100%). The positive level of Skp2 in colorectal group(52.46%) were higher than those in control group. Loss of expression of PTEN and expression of Skp2 were positively correlated with the degree of differentitation, lymphatic metastasis, clinical stage. Moreover, the 5-year survival rates were lower in patients with PTEN negative expression(31.86%) than those with positive expression(83.97%). The 5-year survival rates were lower in patients with positive Skp2 expression(58.33%) than those negative expression(82.76%). The correlation of PTEN with Skp2 was negative (r=-0.445, P=0.001). CONCLUSIONS: Loss of expression of PTEN and expression of Skp2 may be closely related to the carcinogenesis, metastasis and prognosis of colorectal cancer. Joint detection of PTEN and Skp2 may be a good prognostic factor for carcinogenesis, metastasis and pronosis in colorectal cancer.


Zhang H.,Shandong University | Li H.,Fourth Peoples Hospital of Jinan City | Liu X.,Fourth Peoples Hospital of Jinan City | Bi J.,Shandong University
International Journal of Clinical and Experimental Medicine | Year: 2013

Our study investigated the apoptotic mechanism of rat cortical neurons following hypoxia/reperfusion induced endoplasmic reticulum stress (ERS) in vitro and to explore the effect of caspase-9 inhibition on ERS induced apoptosis. Cortical neurons were collected from neonatal rats and cultured in vitro. Immunohistochemistry and immunofluorescence staining for neuron-specific enolase (NSE) were performed to determine the purity of neurons. AnnexinV/PI staining followed by flow cytometry was employed to detect apoptosis rate. Fluorescein isothiocyanate (FITC) staining was done to measure the expression of caspase-3 and -9. Western blot assay was carried out to measure the protein expression of caspase-12, glucose-regulated protein (GRP) 78 and Cytochrome C. The cortical neurons from neonatal rats could be purified and cultured in vitro. In the in vitro hypoxia/reperfusion of cortical neurons (hypoxia for 6 h and reperfusion for 24 h and 48 h), the protein expression of GRP78, caspase-3, 9 and 12 was markedly increased (P < 0.01). Following pre-treatment with caspase-9 inhibitor, the number of apoptotic cells was significantly reduced following hypoxia for 6 and reperfusion for 24 h or 48 h (P < 0.05). Moreover, the expression of caspse-3 and 12 and GRP78 was also significantly reduced in the presence of caspase-9 inhibitor treatment (P < 0.05), but the release of Cytochrome C remained unchanged (P > 0.05). These results demonstrated that ERS is involved in the neuronal apoptosis following in vitro hypoxia/reperfusion, and caspase-9 inhibition can depress the ERS induced apoptosis of neurons.


PubMed | Fourth Peoples Hospital of Jinan City
Type: Journal Article | Journal: Pakistan journal of medical sciences | Year: 2015

Objectives : To determine the risk factors associated with persistent airflow limitation in patients with asthma. Method s: This study was designed and carried out in the department of respiratory medicine, fourth Peoples Hospital of Jinan City, Shandong province, China between Jan 2012 and Dec 2012. Three hundred and six asthma patients participating in the study were divided into persistent airflow limitation group (PAFL) and no persistent airflow limitation group (NPAFL). The patients participated in pulmonary function tests and sputum induction examination. The clinical data including age, gender, onset age, disease course, smoking history, family history, regular corticosteroid inhalation, hospitalization history and presence of atopy were collected. Results : In 306 patients, 128 (40.5%) were included in PAFL group and 178(59.5%) in NPAFL group. Multivariate analysis demonstrated smoking (10 pack-years; OR, 7.1; 95% CI, 1.8 to 31.2), longer asthma duration ( 20years) (OR, 6.3; 95% CI, 1.7 to 28.5), absence of regular corticosteroid inhalation (OR, 3.5; 95% CI, 1.1 to 14.5) and neutrophil in induced sputum65% (OR, 1.8; 95% CI, 1.0 to 2.8) were independent risk factors for PAFL. Conclusions : Smoking, longer asthma duration and increased neutrophil in induced sputum are risk factors for PAFL, while regular corticosteroid inhalation is protective factor. Smoking cessation and regular corticosteroid inhalation may play an important role in preventing the occurrence of persistent airflow limitation group (PAFL).

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