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Li Z.,Fourth Peoples Hospital of Jinan City
The Journal of international medical research

To examine whether there is an association between the serum concentration of vaspin and the presence of carotid plaque in early stage type 2 diabetes mellitus (T2DM). Patients (n = 61) with T2DM within 3 years of diagnosis were divided into those with and those without carotid plaque. Fasting serum vaspin levels, measured by enzymelinked immunosorbent assay, and blood pressure were compared between these two groups and also with an age-matched, apparently healthy control group (n = 26). Fasting serum vaspin concentrations were significantly higher in patients with T2DM without carotid plaque than in controls, but significantly lower in T2DM patients with carotid plaque than in those without. Multivariate logistic regression analysis showed a significant positive association between the presence of carotid plaque and systolic blood pressure and a significant inverse association between the presence of carotid plaque and fasting serum vaspin concentration. A significant inverse association was found, in patients with T2DM within 3 years of diagnosis, between serum vaspin concentration and the presence of carotid plaque. Source

Dong X.-L.,Xian Jiaotong University | Gong Y.,Fourth Peoples Hospital of Jinan City | Chen Z.-Z.,Zibo Central Hospital | Wang Y.-J.,Xian Jiaotong University
Chinese Journal of Integrative Medicine

Objective: To investigate the effect of Delisheng Injection (DLS), a Chinese medicinal compound, DLS combined with cis-platinum (DDP), an active agent used in lung cancer chemotherapy, on a human highly metastatic giant lung carcinoma cell line PGCL3. Methods: The suspended PGCL3 cells at 105 /mL cultured in 96-well tissue culture plates were divided into 4 groups: DLS treatment group (2 μL/mL, 5 μL/mL, 10 μL/mL, 25 μL/mL), DDP treatment group (1 μg/mL, 2 μg/mL, 5 μg/mL, 15 μg/mL), combined DLS with DDP treatment group (DLS:DDP 2 μL/mL:1 μg/mL, 5 μL/mL:2 μg/mL, 10 μL/mL:5 μg/mL, 25 μL/mL:15 μg/mL) and a control group. The cytotoxicity of DLS with different concentrations (2 μL/mL, 5 μL/mL, 10 μL/mL, 25 μL/mL) on PGCL3 cells was determined by 3-(4,5)- dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay. Effect of DLS on adhesion of PGCL-3 cells was tested by cell-matrigel adhesion assay. Chemotactic movement model of transwell camerula was used to determine the effect of DLS on invasion and migration of PGCL-3 cells. Results: Compared with the control group, DLS (2 μL/mL, 5 μL/mL, 10 μL/mL, 25 μL/mL) could significantly decrease cell proliferation, adhesion, invasion and migration abilities (P <0.05). Cell adhesion, invasion and migration abilities were significantly decreased after combination treatment of DLS:DDP (2 μL/mL:1 μg/mL, 5 μL/mL:2 μg/mL, 10 μL/mL:5 μg/mL, 25 μL/mL:15 μg/mL) compared with DDP single-agent treatment (1 μg/mL, 2 μg/mL, 5 μg/mL, 15 μg/mL, P<0.05), respectively. Conclusions: DLS single-agent has a satisfying inhibition effect in PGCL3 cell line and DLS might enhance the inhibition effect of DDP on cancer metastasis. Our research provided a experimental basis about the treatment on highly metastatic lung caner. © 2013 Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag. Source

Qu Q.,Fourth Peoples Hospital of Jinan City | Dai B.,Fourth Peoples Hospital of Jinan City | Yang B.,Fourth Peoples Hospital of Jinan City | Li X.,Fourth Peoples Hospital of Jinan City | And 2 more authors.
BioMed Research International

In the present study, we aimed to investigate the preventive effects of 4-hydroxychalcone (4HCH) on resistant hypertension. We used cryptochrome-null mice, which characteristically show high plasma aldosterone levels, inflammation, and renal injury. The cryptochrome-null mice received high-salt treatment and were treated orally with 4HCH 10 mg/kg, 4HCH 20 mg/kg, and 4HCH 40 mg/kg, respectively. The salt administration in cryptochrome-null mice is able to induce an increase in systolic pressure which is associated with hyperaldosteronism, inflammation, and kidney injury. Treatment with 40 mg/kg 4HCH reduced systolic hypertension, serum IL-1β, and TNF-α levels and suppressed the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-B) and renal injury. The impact of 4HCH on the hyperaldosteronism, inflammation, and kidney injury provides new insights for future development of therapeutic strategies in resistant hypertension. © 2014 Qi Qu et al. Source

Ma C.-C.,Shandong University | Liu A.-J.,Fourth Peoples Hospital of Jinan City | Liu A.-H.,Fourth Peoples Hospital of Jinan City | Zhou X.-Y.,Shandong University of Traditional Chinese Medicine | Zhou S.-N.,Shandong University
Clinical EEG and Neuroscience

Alzheimer's disease (AD) is the most common cause of dementia. Global field synchronization (GFS) can measure functional synchronization in frequency-domain electroencephalogram (EEG) data. The aim of this study is to explore GFS values and its clinical significance for severity of cognitive decline in AD. EEGs were recorded from 37 AD patients and 37 age-matched healthy individuals. GFS values were calculated in delta, theta, alpha, beta 1, beta 2, beta 3, gamma, and full frequency bands. The Montreal Cognitive Assessment (MoCA) and Clinical Dementia Rating scale (CDR) were employed to assess symptom severity in AD patients. Correlation analysis, clustering analysis, and concordance analysis were performed to analyze the relationship between GFS values and MoCA scores in AD patients. GFS values of the beta 1, beta 2, beta 3, and full bands were lower in AD patients than in healthy individuals, and positively correlated with MoCA and CDR scores in the combined group (AD patients and healthy individuals). GFS values were positively correlated with MoCA socres in 3 beta bands and full bands, and with CDR scores in the delta band. There was a good concordance between K-means clustering algorithm calculating of GFS values and MoCA scoring (=.913, P <.001). In conclusion, the present results indicated that GFS can serve as an indicator of cognitive decline or impairment in AD patients. Furthermore, the GFS method of EEG holds considerable promise to distinguish mild cognitive impairment from serious cognitive impairment in patients with AD. © EEG and Clinical Neuroscience Society (ECNS) 2013. Source

Zhang H.,Shandong University | Li H.,Fourth Peoples Hospital of Jinan City | Liu X.,Fourth Peoples Hospital of Jinan City | Bi J.,Shandong University
International Journal of Clinical and Experimental Medicine

Our study investigated the apoptotic mechanism of rat cortical neurons following hypoxia/reperfusion induced endoplasmic reticulum stress (ERS) in vitro and to explore the effect of caspase-9 inhibition on ERS induced apoptosis. Cortical neurons were collected from neonatal rats and cultured in vitro. Immunohistochemistry and immunofluorescence staining for neuron-specific enolase (NSE) were performed to determine the purity of neurons. AnnexinV/PI staining followed by flow cytometry was employed to detect apoptosis rate. Fluorescein isothiocyanate (FITC) staining was done to measure the expression of caspase-3 and -9. Western blot assay was carried out to measure the protein expression of caspase-12, glucose-regulated protein (GRP) 78 and Cytochrome C. The cortical neurons from neonatal rats could be purified and cultured in vitro. In the in vitro hypoxia/reperfusion of cortical neurons (hypoxia for 6 h and reperfusion for 24 h and 48 h), the protein expression of GRP78, caspase-3, 9 and 12 was markedly increased (P < 0.01). Following pre-treatment with caspase-9 inhibitor, the number of apoptotic cells was significantly reduced following hypoxia for 6 and reperfusion for 24 h or 48 h (P < 0.05). Moreover, the expression of caspse-3 and 12 and GRP78 was also significantly reduced in the presence of caspase-9 inhibitor treatment (P < 0.05), but the release of Cytochrome C remained unchanged (P > 0.05). These results demonstrated that ERS is involved in the neuronal apoptosis following in vitro hypoxia/reperfusion, and caspase-9 inhibition can depress the ERS induced apoptosis of neurons. Source

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