Fourth Peoples Hospital of Jinan

Jinan, China

Fourth Peoples Hospital of Jinan

Jinan, China
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Wang J.,Fourth Peoples Hospital of Jinan | Yang M.,Shandong University | Li Y.,Shandong Provincial Military Command | Han B.,Shandong University
Drug Development Research | Year: 2015

Preclinical Research Breast cancer is the most prevalent malignancy in women with more than 1.3 million new cases every year worldwide. Chemotherapy is a critical therapeutic strategy for breast cancer, while chemoresistance remains a major obstacle to treatment success. In the past two decades, significant progress has been achieved in understanding drug resistance in breast cancer, involving drug efflux, alterations in DNA repair pathways, suppression of apoptosis as well as epithelial-mesenchymal transition, and cancer stem cells. However, more effective therapeutic targets and novel biomarkers are still urgently needed to improve the overall survival and refine the therapeutic strategy for breast cancer patients. MicroRNAs (miRNAs) play crucial roles in cellular processes, such as cell differentiation, proliferation, and apoptosis. The recent discovery of miRNAs in malignancy has provided new directions for research on mechanisms underlying response to chemotherapy. Furthermore, several studies have documented that selected miRNAs, such as miR-200c and miR-34a, may influence response to chemotherapy in several tumor types, including breast cancer. The use of miRNAs as therapeutic targets to overcome chemoresistance is currently under investigation. In this review, we summarize the roles of miRNAs in chemoresistance through multiple molecular mechanisms, and highlight the potential diagnostic and therapeutic applications of miRNAs in overcoming breast cancer chemoresistance. © 2015 Wiley Periodicals, Inc.


Liu Q.,Fourth Peoples Hospital of Jinan | Wang T.,Fourth Peoples Hospital of Jinan | Chen R.,Shanghai JiaoTong University | Liu C.,Fourth Peoples Hospital of Jinan | And 3 more authors.
Platelets | Year: 2014

Platelets play an important role in atherothrombosis. As the most common site plaque occurs, left anterior descending artery (LAD) infarct location always associate with poor prognosis. We sought to assess whether mean platelet volume (MPV) could predict LAD infarct location and short-term clinical outcome. In this study, 190 consecutive patients with non-ST-elevation myocardial infarction (NSTEMI) were enrolled. Clinical, electrocardiography and laboratory characteristics were measured. All patients underwent coronary angiography examination and had definite culprit vessel during hospitalization. The results showed that MPV was smaller in patients with a LAD infarct location than that of left circumflex artery or right coronary artery (9.0 ± 1.5 versus 9.8 ± 1.6, p < 0.001). Multivariate analysis also showed that MPV was the only independent factor to predict LAD infarct location [Odds ratio (OR) = 0.65, 95% confidence interval (CI) 0.53-0.80, p < 0.0001] in patients with NSTEMI. B-type natriuretic peptide and electrocardiography were unreliable predictive factors to locate culprit vessel. Receiver operating characteristic curve analysis showed MPV (area under the curve: 0.65, 95% CI 0.56-0.74, p < 0.01) could reliably discriminate those patients with NSTEMI who had a major in-hospital event. Multivariate regression analyses also showed that MPV (OR = 1.46, 95% CI 1.15-1.86, p < 0.01) were predictors of major in-hospital events. In conclusion, MPV was the only factor independently associated with LAD infarct location in patients with non-ST-elevation myocardial infarction. © 2014 Informa UK Ltd.


Wang S.Z.,Fourth Peoples Hospital of jiNan | Ma F.M.,Shandong University of Traditional Chinese Medicine | Zhao J.D.,Shandong University
European Archives of Oto-Rhino-Laryngology | Year: 2013

The purpose of the study was to investigate expressions of nuclear factor-kappa B (NF-κB) and intercellular cell adhesion molecule-1 mRNA (ICAM-1 mRNA) in the nasal mucosa of allergic rhinitis (AR) patients. Expressions of NF-κB and ICAM-1 mRNA were studied using immunohistochemistry and reverse transcription-PCR (RT-PCR) in AR tissues and corresponding normal nasal mucosa. The correlation between NF-κB and ICAM-1 mRNA was studied using linear correlation analysis. The results of immunohistochemistry showed that expression of NF-κB was significantly up-regulated in the nasal mucosa of AR compared with that in normal tissue (P < 0.01), over-expression of NF-κB p50 was found in the cytoplasm and nucleus (P < 0.01), and NF-κB p65 was mainly expressed in the cytoplasm (P < 0.01). ICAM-1 mRNA was strongly expressed in the nasal mucosa of AR compared with that in normal tissue as shown by RT-PCR (P < 0.01). Up-regulation of ICAM-1 mRNA was significantly correlated with over-expressions of NF-κB p50 and NF-κB p65 (r = 0.8995, P < 0.01; r = 0.7601, P < 0.01). In conclusion, NF-κB plays a key role in AR. Excessively activated NF-κB promotes the transcription of ICAM-1 mRNA. ICAM-1 is related to the pathogenesis and development of AR. © 2012 Springer-Verlag.


Wang L.,Modern Medicine | Chang X.,Modern Medicine | Yuan G.,Fourth Peoples Hospital of Jinan | Zhao Y.,Modern Medicine | Wang P.,Fourth Peoples Hospital of Jinan
International Journal of Biological Sciences | Year: 2010

Peptidylarginine deiminase type 4 (PADI4) converts arginine residues into citrulline. The current study focused on the expression of PADI4 in various subtypes of ovary cancers, and this study investigated the effects of estrogen on PADI4 expression in SKOV-3 cells that originated from ovary tumors. We utilized immunohistochemistry, real-time PCR and western blotting to analyze the expression of PADI4 in the tumor tissues and in the cell line that were cultured with estrodial-17β. PADI4 was detected in serious cystadenocarcinoma (n=39, positivity=100%), clear cell cancer (n=7, positivity= 100%), mucinous cystadenocarcinoma (n=6, positivity=100%), dysgerminoma (n=6, positivity=100%), squamous cell tumor (n=6, positivity=100%), sibnet-ring cell carcinoma (n=6, positivity=100%), endodermal sinus tumor (n=6, positivity=100%), germ cell tumors (n=6, positivity=100%) and immature teratoma (n=6, positivity=100%). However, PADI4 was either not detected or detected at low levels in granulosa cell tumor (n=6), malignant thecoma (n=6), ovarian cystadenoma (n=5) and normal ovarian tissue (n=11). For serious cystadenocarcinoma, all of the samples with high PADI4 expression belonged to the T1 and T2 stages of pTMN, whereas all of the samples that exhibited weak or moderate PADI4 expression belonged to the T3 and T4 stages. PADI4 was evenly distributed in the cytoplasm of tumor cells of serious cystadenocarcinoma that were classified as being grade II and III by histopathological scoring. However, PADI4 showed granular cellular distribution in the tumor tissues that were isolated from grade I cystadenocarcinoma. In addition, the PADI4 level was positively related with the ages of the patients that presented with serious adenocarcinoma (p=0.029). Real-time PCR and western blot analyses confirmed that PADI4 was expressed at higher levels in ovarian adenocarcinoma (n=8) compared to ovarian cystadenoma (n=5) (p< 0.05). The study also detected an increased level of PADI4 in SKOV-3 cells that were incubated with estrodial-17β in the range of 10-12 to 10-4M. The results suggest an important role for PADI4 in the tumorigenesis of ovary cancers that are under the regulation of estrogen. © Ivyspring International Publisher.


B L.,Fourth Peoples Hospital of Jinan | T W.,Fourth Peoples Hospital of Jinan | Hn Z.,Fourth Peoples Hospital of Jinan | Ww Y.,Fourth Peoples Hospital of Jinan | And 5 more authors.
BMC Public Health | Year: 2011

Background: The prevalence of hyperuricemia varied in different populations and it appeared to be increasing in the past decades. Recent studies suggest that hyperuricemia is an independent risk factor for cardiovascular disease. However, there has not yet been a systematic analysis of the prevalence of hyperuricemia in China. Methods. Epidemiological investigations on hyperuricemia in China published in journals were identified manually and on-line by using CBMDISC, Chongqing VIP database and CNKI database. Those Reported in English journals were identified using MEDLINE database. Selected studies had to describe an original study defined by strict screening and diagnostic criteria. The fixed effects model or random effects model was employed according to statistical test for homogeneity. Results: Fifty-nine studies were selected, the statistical information of which was collected for systematic analysis. The results showed that the pooled prevalence of hyperuricemia in male was 21.6% (95%CI: 18.9%-24.6%), but it was only 8.6% (95%CI: 8.2%-10.2%) in female. It was found that thirty years was the risk point age in male and it was fifty years in female. Conclusions: The prevalence of hyperuricemia is different as the period of age and it increases after 30 years in male and 50 in female. Interventions are necessary to change the risk factors before the key age which is 30 years in male and 50 in female. © 2011B et al; licensee BioMed Central Ltd.


Wang T.,Fourth Peoples Hospital of Jinan | Liu Q.,Fourth Peoples Hospital of Jinan | Liu C.,Fourth Peoples Hospital of Jinan | Sun L.,Fourth Peoples Hospital of Jinan | And 3 more authors.
Echocardiography | Year: 2014

The aim of this study was to test the hypotheses that epicardial adipose tissue (EAT) can be a marker of severe coronary artery disease in patients with acute myocardial infarction. Overall, 373 cases who underwent coronary angiography were classified into 2 groups by SYNTAX score: low-score and highscore group. EAT was measured by transthoracic echocardiography. Obtained data were compared using Pearson correlation analyses and univariate and multiple logistic regression analysis. The results showed that EAT in the high-score group was significantly greater than in the normal group (5.6 ± 1.1 vs. 4.1 ± 1.0 mm, P < 0.01). EAT had a positive correlation with SYNTAX score (r = 0.61, P < 0.01). Receiver operating characteristic (ROC) curve analyses showed that EAT could reliably discriminate patients with high SYNTAX score (≥33) [AUC: 0.86, 95% confidence interval (CI): 0.822-0.898, P < 0.01]. Multivariate regression analyses showed that EAT was an independent predictor for major inhospital events. These data showed an association between EAT and SYNTAX score. © 2014, Wiley Periodicals, Inc.


Nie H.,Soochow University of China | Xu Y.,Soochow University of China | Liu B.,Fourth Peoples Hospital of Jinan | Zhang Y.,Soochow University of China | And 4 more authors.
International Journal of Geriatric Psychiatry | Year: 2011

Background Dementia has been a major public health problem and mild cognitive impairment is considered the pre-dementia syndrome in recent years. However, there has not yet been a systematic analysis of the prevalence of mild cognitive impairment in China. Objectives The aim of this study was to analyse the prevalence of mild cognitive impairment among the population aged 60 years and older in China. Methods Epidemiological investigations on mild cognitive impairment in China published in journals were identified manually and online by using CBMDISK, ChongqingVIP database and CNKI database. Those reported in English journals were identified using MEDLINE. Selected studies had to describe an original study defined by strict screening and diagnlstic criteria. The fixed effects model or random effects model was employed according to statistical test for homogeneity. Results Twenty-two studies were selected, the statistical information of which was collected for systematic analysis. The results showed that the pooled prevalence of MCI for the elderly population was 12.7% (95% CI: 9.7-16.5%). In eastern and western China, the prevalence of MCI was 9.6 and 14.7%, respectively. There was a higher prevalence of MCI in the illiterate elderly population than in those who received years of education. Conclusions The prevalence of MCI affected by sex, age,education, which was lower in eastern than that in western China. Copyright © 2010 John Wiley & Sons, Ltd.


Xiao S.,Fourth Peoples Hospital Of Jinan | Ma Y.,Fourth Peoples Hospital Of Jinan | Zhu H.,Fourth Peoples Hospital Of Jinan | Sun H.,Fourth Peoples Hospital Of Jinan | And 2 more authors.
Neurological Sciences | Year: 2014

This study aimed to identify potential miRNAs highly associated with the response to brain ischemic stroke. The miRNAs microarray expression profiles data were downloaded from Gene Expression Omnibus database under accession number GSE51586, including three ischemia and three ipsilateral normal samples from mouse brain tissues. Limma package was used to identify differentially expressed miRNAs between ischemia and ipsilateral normal samples. The common target genes of miRNA predicted from TargetScan, PicTar, miRanda and DIANA-microT databases were used as the candidate subset in which functional modules were identified by performing gene ontology enrichment analysis using ClusterProfile. Finally, the miRNA functional synergistic network was constructed by assembling all miRNA synergistic pairs. Fifty-one differentially expressed miRNAs were identified between ischemia and ipsilateral normal samples, including 32 up- and 19 down-regulated miRNAs. Among them, 24 miRNAs can commonly regulate at least one target gene and thus were used to construct a network, which included 274 pairs of co-regulating miRNAs. Further, 242 pairs of miRNAs interaction involving 23 miRNAs were shown to be synergetic in function. Sixteen miRNAs forming 20 miRNAs interaction pairs participated in inflammatory response, such as mir-185 and mir-674-3p. The 16 miRNAs related to inflammatory response during ischemic stroke may provide underlying targets for prevention and treatment of stroke. © 2014, Springer-Verlag Italia.


Zhang H.,Air Force General Hospital of PLA | Gao H.,Fourth peoples hospital of Jinan | Liu C.,Air Force General Hospital of PLA | Kong Y.,Air Force General Hospital of PLA | Wang C.,Air Force General Hospital of PLA
Diagnostic Pathology | Year: 2015

Background: It has been shown that heat shock-related 70-kDa protein 2 (HSPA2), a member of the HSP70 family of heat shock proteins, is important for cancer cell growth and metastasis. However, the status of HSPA2 expression and its prognostic significance in pancreatic cancer remain unknown. Methods: Quantitative reverse transcriptase ploymerase chain reaction (qRT-PCR) was applied to examine HSPA2 messenger RNA (mRNA) expression in 104 pairs of pancreatic cancer tissues and adjacent noncancerous tissues. Statistical analyses were applied to evaluate the diagnostic value and associations of HSPA2 expression with clinicopathological characteristics. Results: HSPA2 mRNA was significantly overexpressed in pancreatic cancer tissues (3.9 ± 0.8) than in adjacent normal tissues (1.1 ± 0.4) (P < 0.001). Clinicopathological analysis showed that HSPA2 expression was significantly correlated with tumor size (P = 0.024), histological differentiation (P = 0.012), TNM stage (P = 0.006), lymph node metastasis (P = 0.043) and serum CA19-9 level (P = 0.046). Moreover, patients with higher HSPA2 expression levels had shorter overall survival time than those with lower HSPA2 expression levels (P = 0.019). Furthermore, Cox regression analyses showed that HSPA2 expression was an independent predictor of overall survival (P = 0.011). Conclusions: Our results suggest that overexpression of HSPA2 in pancreatic cancer is associated with aggressive progression and poor prognosis and that HSPA2 may be served as a prognostic marker.


Zhang J.,Shandong University | Shen C.,Shandong University | Wang L.,Shandong University | Wang L.,Shandong Academy of Sciences | And 5 more authors.
Biochemical and Biophysical Research Communications | Year: 2014

Tumor metastasis is the leading cause of mortality and morbidity of prostate cancer (PCa) patients. Epithelial-mesenchymal transition (EMT) plays a critical role in cancer progression and metastasis. Recent evidence suggested that diabetic patients treated with metformin have lower PCa risk and better prognosis. This study was aimed to investigate the effects of metformin on EMT in PCa cells and the possible microRNA (miRNA)-based mechanisms. MiRNAs have been shown to regulate various processes of cancer metastasis. We herein showed that metformin significantly inhibits proliferation of Vcap and PC-3 cells, induces G0/G1 cell cycle arrest and inhibits invasiveness and motility capacity of Vcap cells. Metformin could inhibit TGF-β-induced EMT in Vcap cells, as manifested by inhibition of the increase of N-cadherin (p = 0.013), Vimentin (p = 0.002) and the decrease of E-cadherin (p = 0.0023) and β-catenin (p = 0.034) at mRNA and protein levels. Notably, we demonstrated significant upregulation of miR30a levels by metformin (P < 0.05) and further experiments indicated that miR30a significantly inhibits proliferation and EMT process of Vcap cells. Interestingly, we identified that SOX4, a previously reported oncogenic transcriptional factor and modulator of EMT, is a direct target gene of miR30a. Finally, we screened the expression of miR30a and SOX4 in 84 PCa cases with radical prostatectomy. Of note, SOX4 overexpression is significantly associated with decreased levels of miR30a in PCa cases. In all, our study suggested that inhibition of EMT by metformin in PCa cells may involve upregulation of miR30a and downregulation of SOX4. © 2014 Elsevier Inc. All rights reserved.

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