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Liu J.,Fourth Peoples Hospital of Guiyang | Zhao B.,Fourth Peoples Hospital of Guiyang | Zhang Y.,Fourth Peoples Hospital of Guiyang | Lin Y.,University of Sichuan | And 2 more authors.
Journal of Biomedical Materials Research - Part A

This study was conducted to investigate whether in vitro chondrogenic differentiated human adipose-derived stem cells (hASCs) can maintain the chondrogenic phenotype in (3-hydroxybutrate-co-3-hydroxyvalerate) (PHBV) scaffolds and whether differentiated hASCs/PHBV construct can produce neocartilage in a heterotopic animal model. hASCs were cultured with or without chondrogenic media in vitro and then seeded on PHBV foams. Differentiated cell/PHBV constructs were subcutaneously implanted in nude mice for 8 or 16 weeks; nondifferentiated cell/PHBV constructs were implanted in the control group. The results in the control group showed no cartilage formation and the disappearance of the scaffold at 8 weeks. Conversely, all differentiated hASCs/PHBV implants kept their original shape throughout 16 weeks. These implants at 16 weeks had stronger chondrocytes-specific histochemical staining than those at 8 weeks, with GAG, total collagen, and compressive moduli increased with implantation time. Cartilage lacunae were observed in all retrieved implants at 16 weeks. The chondrocytes-specific genes were detected by RT-PCR at 16 weeks. The remnants of PHBV were observed in the implants throughout 16 weeks. This study demonstrates that chondrogenic predifferentiated hASCs have the ability to maintain a chondrogenic phenotype in PHBV and that cell/ PHBV constructs can produce neocartilage in a heterotopic site, but the degradation rates of PHBV in different environments needs more investigation. © 2010 Wiley Periodicals, Inc. Source

Zou W.,Fourth Peoples Hospital of Guiyang | Xiao J.,Fourth Peoples Hospital of Guiyang | Long H.,Fourth Peoples Hospital of Guiyang | Wu C.,Fourth Peoples Hospital of Guiyang | Zhou C.-J.,Fourth Peoples Hospital of Guiyang
Chinese Journal of Tissue Engineering Research

BACKGROUND: Clavicle hook plate has been widely applied in the treatment of acromioclavicular joint dislocation and the distal fracture of clavicle and achieved satisfactory effects. The increasing application is associated with the emergency of some complications such as shoulder pain, subacromial impingement, subacromial osteolysis, hook-release, and stress fracture. OBJECTIVE: To analyze the complications and causes after the operations for the acromioclavicular joint dislocation and the distal fracture of clavicle with clavicle hook plate, and to summarize prevention and treatment measures. METHODS: From March 2006 to May 2014, 112 patients with acromioclavicular joint dislocation (64 cases) and the distal fracture of clavicle (48 cases) were cured by clavicle hook plate. Among them, 16 patients with distal fracture of clavicle of Neer type I, 32 patients of Neer type II; and 64 patients with acromioclavicular joint dislocation of Tossy type III. Four patients with superior shoulder suspensory complex double damage. The clinical results of hook plate internal fixation and the complications were retrospectively analyzed. RESULTS AND CONCLUSION: Finally 103 patients were involved in the follow-up analysis and other cases were excluded due to loss of follow-ups. 99 patients exhibited synostosis and no re-dislocation of acromioclavicular joint occurred. The incidence rate of postoperative complications was 30.1%. According toKarlsson criteria, 79 patients got an excellent result, and 16 good. The excellent and good rate was 92.2%. Clavicle hook plate internal fixation is reasonable and effective for the treatment of the acromioclavicular joint dislocation and the distal clavicle fracture. Treatment should be based on the characteristics of individual, and wide concerns are needed to prevent complications. © 2014, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved. Source

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