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Yang H.,PLA Fourth Military Medical University | Gao L.-N.,PLA Fourth Military Medical University | An Y.,PLA Fourth Military Medical University | Hu C.-H.,PLA Fourth Military Medical University | And 4 more authors.

Gingival tissue-derived mesenchymal stem cells (MSCs) were recently identified and characterized as having multipotential differentiation and immunomodulatory properties invitro and invivo, and they represent new postnatal stem cell types for cytotherapy and regenerative medicine. However, the utility of gingival MSCs (GMSCs) as alternatives to periodontal ligament stem cells (PDLSCs), which have been demonstrated to be effective but with limited cell availability and reduced clinical feasibility, for periodontal regeneration in a previously diseased/inflamed environment remains obscure. In this study, patient-matched human GMSCs and PDLSCs were evaluated in terms of their colony-forming ability, proliferative capacity, cell surface epitopes, multi-lineage differentiation potentials, and related gene expression when incubated in different designed culture conditions, with or without the presence of inflammatory cytokines. An invivo ectopic transplantation model using transplants from inflammatory cytokine-treated or untreated cells was applied to assess bone formation. We found that cells derived from both tissues expressed MSC markers, including CD146, CD105, CD90, CD29, and STRO-1. Both cells successfully differentiated under osteogenic, adipogenic, and chondrogenic microenvironments; PDLSCs displayed a more effective differentiation potential in all of the incubation conditions compared to GMSCs (P<0.01). Although inflammatory cytokine-treated GMSCs and PDLSCs are inferior to normally cultured, patient and tissue-matched cells in terms of their osteogenic capacity and regenerative potential (P<0.05), they retain the capacity for osteoblastic and adipose differentiation, as well as ectopic bone formation, similar to what has been demonstrated for other MSCs. Interestingly, GMSCs exhibited fewer inflammation-related changes in terms of osteogenic potential invitro and bone formation invivo compared to PDLSCs (P<0.01). These results suggest that both gingiva and PDL tissues are putative cell sources for future cytotherapeutic applications. Whether GMSCs act as an adjunctive or alternative cell source for cytotherapy of inflammatory periodontal disease warrants further investigation. © 2013 Elsevier Ltd. Source

Wang Q.,Institute of Hematology | Yan Y.,Fourth Military Medical University Email Yanyping@Fmmueducn | Ji Z.,Fourth Military Medical University | Ni Q.,Institute of Hematology | And 6 more authors.
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi

OBJECTIVE: To evaluate the quality of life and influencing factors on patients with multiple myeloma (MM).METHODS: 227 MM cases were selected at 5 hospitals in Xi'an from August, 2010 to March, 2013. QLQ-C30 was used to evaluate the quality of life of MM patients, and their norms were as control. Factors which influencing the quality of life were investigated and analyzed with SPSS 17.0 software.RESULTS: The total score of quality of life in MM patients was 49.0±21.7 which was lower than the norms (60.7±23.4). The scores on fatigue, nausea, vomiting, pain, short of breath, disturbance on sleeping, losing appetite, constipation, other symptoms and financial difficulty were significantly higher than data of the norms (P < 0.05). Factors as being elderly (especially those older than 70), under higher proportion of medical costs on their own expense or financial difficulty etc., had major influences on the quality of life (P < 0.05) of MM patients who in particular having worse quality of life when in worsening clinical ISS stage (P < 0.05). Low level of hemoglobin, high level of serum calcium and globulin all significantly reduced the quality of life of the MM patients (P < 0.05).CONCLUSION: The quality of life of MM patients was significantly lower than the normal people or patients with other tumors. Fatigue, pain, and financial difficulty were main influencing factors on the quality of life of MM patients. The assessment on the effects of treatment should relate to the improvement of hemoglobin, serum calcium and globulin, which could all improve the quality of life of MM patients. Source

Wang M.-Q.,PLA Fourth Military Medical University | He J.-J.,Fourth Military Medical University | Chen C.-S.,Shaanxi University of Chinese Medicine | Widmalm S.E.,Karolinska Institutet
Cranio - Journal of Craniomandibular Practice

The aim of this study was to test the hypothesis that condylar and occlusion asymmetry are not associated. For each of 22 skulls, the asymmetry of condyles was graded by one examiner and the asymmetry of occlusion by another examiner, both blinded to each other's evaluation, as 0 = symmetrical, 1 = mild asymmetrical and 2 = severe asymmetrical. There were 18 condyles graded the same as to their occlusion, but in four, the grades differed by one degree. Nine were graded symmetrical, seven were mild, and six were graded severely asymmetrical condyles. The corresponding figures for occlusion were: 10 were graded symmetrical, seven were graded mildly asymmetrical, and five were graded severely asymmetrical occlusion. The relation between occlusion and condylar asymmetry was tested using Goodman-Kruskal's gamma and was found to be 0.970 (p<0.001). The null hypothesis was not supported. The results indicate that asymmetry of occlusion and condyles are associated, which indicates the need for further studies on larger samples, and in vivo studies. Source

Tu Y.,Fourth Military Medical University | Gao X.,Fourth Military Medical University | Gao X.,Xian Medical University | Gao X.,Shanghai Institute of Technology | And 7 more authors.
Cancer Research

Malignant gliomas are the most common central nervous system tumors and the molecular mechanism driving their development and recurrence is still largely unknown, limiting the treatment of this disease. Here, we show that restoring the expression of miR-218, a microRNA commonly downregulated in glioma, dramatically reduces the migration, invasion, and proliferation of glioma cells. Quantitative reverse transcription PCR and Western blotting analysis revealed that expression of the stem cell-promoting oncogene Bmi1 was decreased after overexpression of miR-218 in glioma cells. Mechanistic investigations defined Bmi1 as a functional downstream target of miR-218 through which miR-218 ablated cell migration and proliferation. We documented that miR-218 also blocked the self-renewal of glioma stem-like cells, consistent with the suggested role of Bmi1 in stem cell growth. Finally, we showed that miR-218 regulated a broad range of genes involved in glioma cell development, including Wnt pathways that suppress glioma cell stem-like qualities. Taken together, our findings reveal miR-218 as a tumor suppressor that prevents migration, invasion, proliferation, and stemlike qualities in glioma cells. © 2013 American Association for Cancer Research. Source

Zheng M.,Fourth Military Medical University | Zhang X.,PLA Fourth Military Medical University | Guo H.,Fourth Military Medical University | Fan C.,PLA Fourth Military Medical University | And 2 more authors.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology

OBJECTIVE: To investigate the expression of CD147 in CD4⁺ tumor infiltrating lymphocytes and explore the clinical significance of CD147 transcripts in breast cancer patients by comparing CD147 level in healthy controls' peripheral blood, breast cancer patients' peripheral blood, lymph nodes and CD4⁺ tumor infiltrating lymphocytes.METHODS: CD147 expression data were derived by Robust Multi-array Averaging method from three different microarrays, GSE14308, GSE36765 and GSE6532. ANOVA, Kaplan-Meier curve and multivariate Cox proportional hazards model were used for the statistical analysis.RESULTS: The expression of CD147 in Th1, Th2 and Th17 cells was 8-12 times higher than that in Th0 cells in normal C57BL/6J mice. The expression level of CD147 in the CD4⁺ tumor infiltrating lymphocytes from breast cancer patients was significantly higher than that in the CD4⁺ lymphocytes from peripheral blood of healthy donors, peripheral blood and axillary lymph nodes of breast cancer patients. High expression of CD147 was significantly associated with an increased risk of relapse in the Kaplan-Meier curve analysis (Log-Rank, P=0.019) and multivariate analysis (hazard ratio=1.764; 95% confidence interval: 1.088-2.859).CONCLUSION: CD147 might promote the progress of breast cancer through up-regulating CD4⁺ tumor infiltrating lymphocytes and aggravating inflammatory reaction. Source

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