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Wu L.-A.,Fourth Hospital of xiAn City | Zhang L.,Xian Jiaotong University | Wang C.-Y.,Fourth Hospital of xiAn City | Yang X.-G.,Fourth Hospital of xiAn City
International Journal of Ophthalmology | Year: 2010

• AIM: To observe the corneal endothelial cells morphology at the central and incision cornea of diabetes patient after phacoemulsification, and find out the regularity of corneal endothelial cells morphologic change and some related affected factors. • METHODS: Twenty-eight cases 32 eyes with age-related cataract and non-insulin dependent diabetes mellitus (diabetes group) and 31 cases 32 eyes age-related cataract (control group) were randomly chosen in this research. The phacoemulsification was performed through the clear corneal combined with the foldable intraocular lens implantation. The cell density (CD), proportion of hexagonal cell, coefficient of variation (CV) of the corneal endothelial cells were examined preoperatively and one week, one month, three months postoperatively. • RESULTS: In the two groups, the level of postoperative CD, proportion of hexagonal cell decreased gradually, and the level of CV increased. Compared with the control group, corneal endothelial cells in diabetes group had a significant reduction in proportion of hexagonal cell (P < 0. 01) and increase in CV (P < 0.01). There existed a significant difference in CD (P < 0.01), proportion of hexagonal cell (P < 0.01), CV (P < 0.01) at three months postoperatively between the two groups, and especially more remarkable change was found in the diabetes group. Morphology of endothelial cells at the incision corneal showed a significant lower CD (P < 0.05 at one week, one month postoperatively; P < 0.01 at three months postoperatively) and proportion of hexagonal cell (P < 0.01 at one week, one month postoperatively; P < 0.05 at three months postoperatively), higher CV (P < 0.01) than those at the central cornea at postoperative different time. • CONCLUSION: There exists a continued cell loss in the corneal endothelium after phacoemulsification. The morphology of corneal endothelial cells in diabetes group, compared with that in the control group, present significantly abnormality. So the endothelial cells in diabetes group are easier to be damaged than those in control group during this surgery, and the velocity and validity of recovery in diabetes group are lower comparatively. In phacoemulsification through the clear corneal, the endothelial cells suffer from energy and mechanic injury. We should pay more attention to the mechanic injury for diabetes patient, because it can lead to more corneal endothelial cell loss. Source

Wang L.-Q.,Xian Medical College | Yu X.-W.,Xian Jiaotong University | Yan C.-F.,Fourth Hospital of xiAn City | Wang X.,Xian Medical College
International Journal of Molecular Sciences | Year: 2010

The nuclear factor kappa B is widely expressed in the distinct subpopulations of chorionic villi and deciduas of first-trimester pregnancies. We examined the cellular distribution and expression of nuclear factor kappa B in the human first-trimester chorionic villi and deciduas of women with early spontaneous miscarriage and viable pregnancy by confocal laser scanning microscope and immunohistochemistry. There is a greater nuclear translocation of nuclear factor kappa B is restricted to villous stromal cells, decidual stromal cells, glandular epithelial cells and vessel endothelial cells in early spontaneous miscarriage than in viable pregnancies. Collectively these observations suggest that over-activation of nuclear factor kappa B has a relationship with early spontaneous miscarriages. © 2010 by the authors; licensee Molecular Diversity Preservation International. Source

Lin Q.-Q.,Xian Jiaotong University | Yan C.-F.,Fourth Hospital of xiAn City | Lin R.,Xian Jiaotong University | Zhang J.-Y.,Xian Jiaotong University | And 3 more authors.
Cytokine | Year: 2012

Sirtuin1 (SIRT1), a NAD+-dependent deacetylase, not only regulates lipid and glucose homeostasis, but also involves the regulation of proinflammatory cytokine involved in inflammation-associated diseases. The activation of CD40 triggers inflammation that plays a crucial role in the development of many chronic inflammatory diseases including obesity. Growing evidence indicated that SIRT1 exerts anti-inflammatory properties by suppressing proinflammatory cytokines production. However, the effect of SIRT1 on the expression of CD40 in adipocytes has not yet been fully elucidated. The present study showed that SIRT1 expressed both in the nucleus and cytoplasm of 3T3-L1 adipocytes. TNF-α significantly reduced the expression of SIRT1 mRNA and protein and increased the expression of CD40 mRNA and protein in time- and concentration-dependent manners. Overexpression of SIRT1 or SIRT1 activation by resveratrol obviously attenuated the expression of CD40 induced by TNF-α in 3T3-L1 adipocytes, whereas knockdown of SIRT1 or SIRT1 inhibition by nicotinamide and sirtinol significantly enhanced TNF-α-induced expression of CD40. Furthermore, overexpression of SIRT1 or SIRT1 activation by resveratrol diminished TNF-α-induced acetylation of NF-κBp65, while knockdown of SIRT1 or SIRT1 inhibition by nicotinamide and sirtinol augmented TNF-α-induced acetylation of NF-κBp65 in 3T3-L1 adipocytes. NF-κB inhibitor PDTC reduced TNF-α-induced mRNA and protein expression of CD40 in 3T3-L1 adipocytes. The combination treatment of resveratrol and PDTC significantly reduced TNF-α-induced expression of CD40, and the inhibitory effects were higher than that of the single treatment. Taken together, SIRT1 exerts anti-inflammatory property by regulating TNF-α-induced expression of CD40 partially through the NF-κB pathway in 3T3-L1 adipocytes. More importantly, the regulation of SIRT1 on the expression of CD40 provides new insight to understand the anti-inflammatory effects of SIRT1. © 2012 Elsevier Ltd. Source

Yang L.,Xian Jiaotong University | Zhang J.,Xian Jiaotong University | Yan C.,Fourth Hospital of xiAn City | Zhou J.,Xian Jiaotong University | And 7 more authors.
Cellular Physiology and Biochemistry | Year: 2012

Background: Compelling evidence suggests that SIRT1, NAD +-dependent class III protein deacetylase, plays an important role in the prevention and treatment of atherosclerosis by counteracting infammation. Cluster of differentiation 40(CD40), as a pro-infammatory cytokine, has been shown to participate in the pathophysiology of atherosclerosis. The relationship between SIRT1 and CD40, however, remained elusive. The present study was thus designed to explore the potential effect of SIRT1 on CD40 expression induced by tumor necrosis factor-α (TNF-α) and to disclose the underlying mechanism in CRL-1730 endothelial cells. Methods: mRNA and protein expressions were identifed by quantitative real-time PCR and Western blot respectively. Subcellular localization of SIRT1 was detected by immunofuorescence analysis. SIRT1 small-interfering RNA (siRNA) was carried out for mechanism study. Results: TNF-α reduced SIRT1 expression and induced CD40 expression in CRL-1730 endothelial cells in a time- and concentration-dependent manner. Pretreatment with resveratrol (a potent SIRT1 activator) inhibited TNF-α-induced CD40 expression, while pretreatment with nicotinamide (class β HDACs inhibitor nicotinamide) or sirtinol (a known SIRT1 inhibitor), especially SIRT1 siRNA signifcantly augmented TNF-α-induced CD40 expression. The frther sudy idicated that PDTC (NF-κB inhibitor) pretreatment attenuated TNF-α-induced CD40 expression, and SIRT1 siRNA signifcantly augmented TNF-α-induced acetylated-NF-κB p65 (Lys310) expression. Conclusion: The present study provides the direct evidence that SIRT1 can inhibit TNF-α-induced CD40 expression in CRL-1730 endothelial cells by deacetylating the RelA/p65 subunit of NF-κB at lysine 310, which provides new insights into understanding of the antiinfammatory and anti-athroscerotic actions of SIRT1. Copyright © 2012 S. Karger AG, Basel. Source

Wang H.,Northwest University, China | Wu Y.,Fourth Hospital of xiAn City | Song J.-F.,Northwest University, China
Biosensors and Bioelectronics | Year: 2015

In this work, the adsorption of human serum albumin (HSA) on the bare multiwall carbon nanotube (MWNT) was investigated by a new electrochemical method, termed as zero current potentiometry. For this, a MWNT strip was prepared by directly adhering MWNTs on the transparent adhesive tape surface. Moreover, when HSA adsorbed onto MWNT at the MWNT/solution interface, an interface potential Ψ yielded. The interface potential Ψ as the zero current potential Ezcp simply related to it was monitored by zero current potentiometry. The relationship between the zero current potential Ezcp, the HSA concentration and others was established in simple stoichiometric relation. Based on this, both the adsorption of HSA on MWNT and the HSA determination can be studied. For the HSA determination, the theoretic conclusion consisted with experimental results. The zero current potential Ezcp was proportional to the HSA concentration in the range of 2.8×10-8-3.4×10-7M with the limit of detection 2×10-8M. The linear regression equation was Ezcp/V (vs, SCE)=(0.159±0.01)+(0.358±0.02)×106CHSA (μM). This determination was fast, high sensitive and good selective. © 2015 Elsevier B.V. Source

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