Fourth Hospital of xiAn City

Fengcheng, China

Fourth Hospital of xiAn City

Fengcheng, China
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Lin Q.-Q.,Xi'an Jiaotong University | Yan C.-F.,Fourth Hospital of Xian City | Lin R.,Xi'an Jiaotong University | Zhang J.-Y.,Xi'an Jiaotong University | And 3 more authors.
Cytokine | Year: 2012

Sirtuin1 (SIRT1), a NAD+-dependent deacetylase, not only regulates lipid and glucose homeostasis, but also involves the regulation of proinflammatory cytokine involved in inflammation-associated diseases. The activation of CD40 triggers inflammation that plays a crucial role in the development of many chronic inflammatory diseases including obesity. Growing evidence indicated that SIRT1 exerts anti-inflammatory properties by suppressing proinflammatory cytokines production. However, the effect of SIRT1 on the expression of CD40 in adipocytes has not yet been fully elucidated. The present study showed that SIRT1 expressed both in the nucleus and cytoplasm of 3T3-L1 adipocytes. TNF-α significantly reduced the expression of SIRT1 mRNA and protein and increased the expression of CD40 mRNA and protein in time- and concentration-dependent manners. Overexpression of SIRT1 or SIRT1 activation by resveratrol obviously attenuated the expression of CD40 induced by TNF-α in 3T3-L1 adipocytes, whereas knockdown of SIRT1 or SIRT1 inhibition by nicotinamide and sirtinol significantly enhanced TNF-α-induced expression of CD40. Furthermore, overexpression of SIRT1 or SIRT1 activation by resveratrol diminished TNF-α-induced acetylation of NF-κBp65, while knockdown of SIRT1 or SIRT1 inhibition by nicotinamide and sirtinol augmented TNF-α-induced acetylation of NF-κBp65 in 3T3-L1 adipocytes. NF-κB inhibitor PDTC reduced TNF-α-induced mRNA and protein expression of CD40 in 3T3-L1 adipocytes. The combination treatment of resveratrol and PDTC significantly reduced TNF-α-induced expression of CD40, and the inhibitory effects were higher than that of the single treatment. Taken together, SIRT1 exerts anti-inflammatory property by regulating TNF-α-induced expression of CD40 partially through the NF-κB pathway in 3T3-L1 adipocytes. More importantly, the regulation of SIRT1 on the expression of CD40 provides new insight to understand the anti-inflammatory effects of SIRT1. © 2012 Elsevier Ltd.


Wang W.,Xi'an Jiaotong University | Yan C.,Fourth Hospital of xiAn City | Zhang J.,Xi'an Jiaotong University | Lin R.,Xi'an Jiaotong University | And 5 more authors.
Apoptosis | Year: 2013

Sirtuin 1 (SIRT1), a NAD+-dependent class III histone deacetylase, participates in regulating cellular apoptosis, senescence and metabolism by deacetylating histones and multiple transcription factors. In this study, we aimed to determine the effect of SIRT1 on the apoptosis of vascular adventitial fibroblasts (VAFs) and related signaling pathways. SIRT1 was found in the nucleus of VAFs and translocated into the cytoplasm in response to tumor necrosis factor-α (TNF-α). Moreover, SIRT1 protein expression was reduced in VAFs stimulated with TNF-α. In addition, TNF-α increased the apoptosis of VAFs. Activation of SIRT1 by resveratrol (RSV) or overexpression of SIRT1 attenuated TNF-α-induced VAF apoptosis by decreasing the percentage of apoptotic cells and cleaved caspase-3 protein expression and increasing the Bcl-2/Bax ratio. In contrast, inhibition of SIRT1 by sirtinol/nicotinamide or knockdown of SIRT1 enhanced apoptosis of VAFs. On the other hand, knockdown of FoxO1 reduced TNF-α-induced VAF apoptosis. SIRT1 interacted with FoxO1 in VAFs by the co-immunoprecipitation assay. Further study showed that RSV or SIRT1 overexpression decreased acetylated-FoxO1 (Ac-FoxO1) protein expression in VAFs stimulated with TNF-α. Knockdown of SIRT1 resulted in an increase in Ac-FoxO1 protein expression. Taken together, these findings indicate that SIRT1 inhibits the apoptosis of VAFs, whereas FoxO1 promotes VAF apoptosis. Furthermore, the inhibitory effect of SIRT1 on VAF apoptosis is partly mediated by the deacetylation of FoxO1. © 2013 Springer Science+Business Media New York.


Wu L.-A.,Fourth Hospital of Xian City | Zhang L.,Xi'an Jiaotong University | Wang C.-Y.,Fourth Hospital of Xian City | Yang X.-G.,Fourth Hospital of Xian City
International Journal of Ophthalmology | Year: 2010

• AIM: To observe the corneal endothelial cells morphology at the central and incision cornea of diabetes patient after phacoemulsification, and find out the regularity of corneal endothelial cells morphologic change and some related affected factors. • METHODS: Twenty-eight cases 32 eyes with age-related cataract and non-insulin dependent diabetes mellitus (diabetes group) and 31 cases 32 eyes age-related cataract (control group) were randomly chosen in this research. The phacoemulsification was performed through the clear corneal combined with the foldable intraocular lens implantation. The cell density (CD), proportion of hexagonal cell, coefficient of variation (CV) of the corneal endothelial cells were examined preoperatively and one week, one month, three months postoperatively. • RESULTS: In the two groups, the level of postoperative CD, proportion of hexagonal cell decreased gradually, and the level of CV increased. Compared with the control group, corneal endothelial cells in diabetes group had a significant reduction in proportion of hexagonal cell (P < 0. 01) and increase in CV (P < 0.01). There existed a significant difference in CD (P < 0.01), proportion of hexagonal cell (P < 0.01), CV (P < 0.01) at three months postoperatively between the two groups, and especially more remarkable change was found in the diabetes group. Morphology of endothelial cells at the incision corneal showed a significant lower CD (P < 0.05 at one week, one month postoperatively; P < 0.01 at three months postoperatively) and proportion of hexagonal cell (P < 0.01 at one week, one month postoperatively; P < 0.05 at three months postoperatively), higher CV (P < 0.01) than those at the central cornea at postoperative different time. • CONCLUSION: There exists a continued cell loss in the corneal endothelium after phacoemulsification. The morphology of corneal endothelial cells in diabetes group, compared with that in the control group, present significantly abnormality. So the endothelial cells in diabetes group are easier to be damaged than those in control group during this surgery, and the velocity and validity of recovery in diabetes group are lower comparatively. In phacoemulsification through the clear corneal, the endothelial cells suffer from energy and mechanic injury. We should pay more attention to the mechanic injury for diabetes patient, because it can lead to more corneal endothelial cell loss.


Yang L.,Xi'an Jiaotong University | Zhang J.,Xi'an Jiaotong University | Yan C.,Fourth Hospital of Xian City | Zhou J.,Xi'an Jiaotong University | And 7 more authors.
Cellular Physiology and Biochemistry | Year: 2012

Background: Compelling evidence suggests that SIRT1, NAD +-dependent class III protein deacetylase, plays an important role in the prevention and treatment of atherosclerosis by counteracting infammation. Cluster of differentiation 40(CD40), as a pro-infammatory cytokine, has been shown to participate in the pathophysiology of atherosclerosis. The relationship between SIRT1 and CD40, however, remained elusive. The present study was thus designed to explore the potential effect of SIRT1 on CD40 expression induced by tumor necrosis factor-α (TNF-α) and to disclose the underlying mechanism in CRL-1730 endothelial cells. Methods: mRNA and protein expressions were identifed by quantitative real-time PCR and Western blot respectively. Subcellular localization of SIRT1 was detected by immunofuorescence analysis. SIRT1 small-interfering RNA (siRNA) was carried out for mechanism study. Results: TNF-α reduced SIRT1 expression and induced CD40 expression in CRL-1730 endothelial cells in a time- and concentration-dependent manner. Pretreatment with resveratrol (a potent SIRT1 activator) inhibited TNF-α-induced CD40 expression, while pretreatment with nicotinamide (class β HDACs inhibitor nicotinamide) or sirtinol (a known SIRT1 inhibitor), especially SIRT1 siRNA signifcantly augmented TNF-α-induced CD40 expression. The frther sudy idicated that PDTC (NF-κB inhibitor) pretreatment attenuated TNF-α-induced CD40 expression, and SIRT1 siRNA signifcantly augmented TNF-α-induced acetylated-NF-κB p65 (Lys310) expression. Conclusion: The present study provides the direct evidence that SIRT1 can inhibit TNF-α-induced CD40 expression in CRL-1730 endothelial cells by deacetylating the RelA/p65 subunit of NF-κB at lysine 310, which provides new insights into understanding of the antiinfammatory and anti-athroscerotic actions of SIRT1. Copyright © 2012 S. Karger AG, Basel.


Wang L.-Q.,Xian Medical College | Yu X.-W.,Xi'an Jiaotong University | Yan C.-F.,Fourth Hospital of Xian City | Wang X.,Xian Medical College
International Journal of Molecular Sciences | Year: 2010

The nuclear factor kappa B is widely expressed in the distinct subpopulations of chorionic villi and deciduas of first-trimester pregnancies. We examined the cellular distribution and expression of nuclear factor kappa B in the human first-trimester chorionic villi and deciduas of women with early spontaneous miscarriage and viable pregnancy by confocal laser scanning microscope and immunohistochemistry. There is a greater nuclear translocation of nuclear factor kappa B is restricted to villous stromal cells, decidual stromal cells, glandular epithelial cells and vessel endothelial cells in early spontaneous miscarriage than in viable pregnancies. Collectively these observations suggest that over-activation of nuclear factor kappa B has a relationship with early spontaneous miscarriages. © 2010 by the authors; licensee Molecular Diversity Preservation International.


Song J.,Xi'an Jiaotong University | Wu Y.,Fourth Hospital of xiAn City | Nie F.,Northwest University, China | Wang B.,Xi'an Jiaotong University | And 7 more authors.
Chinese Journal of Medical Genetics | Year: 2015

Objective To identify the candidate chromosomal region for congenital preauricular fistula (CPF) through analysis of an affected Chinese family. Methods Conventional linkage analysis using short tandem repeats (STR) markers was performed to investigate three chromosomal regions 8q11. 1-q13. 3, 1q32-q34. 3 and 14q31. l-q31. 3. Results None of 16 STRs could attain a LOD score of more than - 2. 0 (theta=0). Therefore, the three regions were all excluded as the candidate region for the disease. Conclusion CPF features high genetic heterogeneity. The family may have a causative gene elsewhere. Whole-genome-based study is needed to identify its genetic etiology.


Sun W.-T.,Fourth Hospital of Xian City | Lei C.-L.,Fourth Hospital of Xian City | Bi C.-C.,Fourth Hospital of Xian City | Wang R.,Fourth Hospital of Xian City
International Journal of Ophthalmology | Year: 2010

• AIM: To observe clinical effects of intravitreous injection of avastin (bevacizumab) in central retinal vein occlusion (CRVO) with macular edema(ME). • METHODS: Indirect ophthalmoscopy, optical coherence tomography (OCT) and fundus fluorescein angiography (FFA) confirmed that 72 patients 75 eyes had CRVO with ME in our hospital from April 2007 to October 2009. Group A had 38 patients 39 eyes, group A1 of 20 patients 21 eyes received intravitreal injection of 1.25mg(0.05mL) avastin, group A2 of 18 patients with 18 eyes received intravitreal injection of 1.25mg(0.05mL) avastin and the treatment was repeated after 4 weeks. Group B had 34 patients 36 eyes, group B1 of 16 patients 16 eyes received intravitreal injection of 2.0mg(0.08mL) avastin, group B2 of 18 patients 20 eyes received intravitreal injection of 2.0mg (0.08mL) avastin and the treatment was repeated after 4 weeks. Visual acuity, intraocular pressure and fundus were compared before each treatment, 1 week, 2, 4 weeks after treatment. The performance of FFA and OCT macular retinal thickness measurement were compared before treatment and 4 weeks after treatment. • RESULTS: Out of 72 patients 75 eyes, visual acuity of 58 patients 61 eyes was improved and macular edema reduced . The difference between group A1 and group A2, group B1 and group B2 was significant (P < 0.01). There was no statistically significant difference between group A1 and group B1, group A2 and group B2. • CONCLUSION; Intravitreal injection of avastin can improve the CRVO in patients with ME secondary to a high degree of visual acuity and ME, repeated treatment effect is more obvious. But the increase intravitreal injection of avastin did not significantly reduce the high degree of ME.


Wang H.,Northwest University, China | Wu Y.,Fourth Hospital of Xian City | Song J.-F.,Northwest University, China
Biosensors and Bioelectronics | Year: 2015

In this work, the adsorption of human serum albumin (HSA) on the bare multiwall carbon nanotube (MWNT) was investigated by a new electrochemical method, termed as zero current potentiometry. For this, a MWNT strip was prepared by directly adhering MWNTs on the transparent adhesive tape surface. Moreover, when HSA adsorbed onto MWNT at the MWNT/solution interface, an interface potential Ψ yielded. The interface potential Ψ as the zero current potential Ezcp simply related to it was monitored by zero current potentiometry. The relationship between the zero current potential Ezcp, the HSA concentration and others was established in simple stoichiometric relation. Based on this, both the adsorption of HSA on MWNT and the HSA determination can be studied. For the HSA determination, the theoretic conclusion consisted with experimental results. The zero current potential Ezcp was proportional to the HSA concentration in the range of 2.8×10-8-3.4×10-7M with the limit of detection 2×10-8M. The linear regression equation was Ezcp/V (vs, SCE)=(0.159±0.01)+(0.358±0.02)×106CHSA (μM). This determination was fast, high sensitive and good selective. © 2015 Elsevier B.V.


Chang Y.H.,Fourth Hospital of Xian City
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban | Year: 2013

To explore effects and possible mechanisms of curcumin on hypoxia induced epithelial-mesenchymal transition (EMT) in hepatocellular carcinoma cell line HepG2. HepG2 cells were divided to 3 groups, i.e., the normal control group, the CoCl2 group, and the CoCl2 plus 10 micromol/L curcumin group. The proliferation of HepG2 was determined using MTT assay. The migration of HepG2 was detected by wound healing assay.The mRNA expression of hypoxia-inducible factor-1 (HIF-1alpha) was evaluated with real-time RT-PCR. The protein expressions of HIF-1alpha, epithelial-cadherin (E-cadherin), and vimentin were determined using Western blot. Compared with the normal control group, the proliferation and migration of HepG2 cells under CoCl2-induced hypoxia significantly increased, the expression of HIF-1alpha was up-regulated, and the expression of E-cadherin protein was obviously down-regulated, and the expression of vimentin significantly increased (all P < 0.05). Intervention by curcumin significantly inhibited the proliferation and migration of hypoxic HepG2 cells, and expressions of HIF-1alpha and vimentin decreased, and the expression of E-cadherin was up-regulated, showing statistical difference when compared with those of the CoCl2 group (P < 0.05). There was no statistical difference in HIF-1alpha mRNA expression among the 3 groups (P > 0.05). Curcumin could reverse the proliferation and migration of HepG2 cells under CoCl2-induced hypoxia condition, which might be associated with inhibiting up-regulated expressions of HIF-1alpha protein and EMT.


PubMed | Fourth Hospital of Xian City
Type: Journal Article | Journal: Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine | Year: 2013

To explore effects and possible mechanisms of curcumin on hypoxia induced epithelial-mesenchymal transition (EMT) in hepatocellular carcinoma cell line HepG2.HepG2 cells were divided to 3 groups, i.e., the normal control group, the CoCl2 group, and the CoCl2 plus 10 micromol/L curcumin group. The proliferation of HepG2 was determined using MTT assay. The migration of HepG2 was detected by wound healing assay.The mRNA expression of hypoxia-inducible factor-1 (HIF-1alpha) was evaluated with real-time RT-PCR. The protein expressions of HIF-1alpha, epithelial-cadherin (E-cadherin), and vimentin were determined using Western blot.Compared with the normal control group, the proliferation and migration of HepG2 cells under CoCl2-induced hypoxia significantly increased, the expression of HIF-1alpha was up-regulated, and the expression of E-cadherin protein was obviously down-regulated, and the expression of vimentin significantly increased (all P < 0.05). Intervention by curcumin significantly inhibited the proliferation and migration of hypoxic HepG2 cells, and expressions of HIF-1alpha and vimentin decreased, and the expression of E-cadherin was up-regulated, showing statistical difference when compared with those of the CoCl2 group (P < 0.05). There was no statistical difference in HIF-1alpha mRNA expression among the 3 groups (P > 0.05).Curcumin could reverse the proliferation and migration of HepG2 cells under CoCl2-induced hypoxia condition, which might be associated with inhibiting up-regulated expressions of HIF-1alpha protein and EMT.

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