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Wang D.,Tianjin Fourth Central Hospital
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery | Year: 2010

Bone marrow mesenchymal stem cells (BMSCs) play an important role in repairing nerve injury, meanwhile external temperature has significant effect on BMSCs transplantation, proliferation, and differentiation. To investigate the effect of BMSCs transplantation and mild hypothermia on repair of rat spinal cord injury (SCI). Forty-five female adult SD rats (weighing 200-250 g) were made the models of hemitransection SCI and divided randomly into 3 groups according to different treatments: group A (SCI group), group B (BMSCs transplantation group), and group C [BMSCs transplantation combined with mild hypothermia (33-35 degrees C) group]. At 1, 2, 4, 6, and 8 weeks after injury, the function of hind limb was evaluated with Basso Beattie and Bresnahan (BBB) score and inclined plane test. At 4 weeks after injury, histopathology and BrdU immunohistochemistry staining were performed. At 8 weeks after injury, horseradish peroxidase (HRP) retrograde nerve trace and transmission electron microscope (TEM) testing were performed to observe the regeneration of axon. After 4 weeks, the function of hind limb obviously recovered in groups B and C, there were significant differences in BBB score between groups B, C and group A (P < 0.05), between group B and group C (P < 0.05). There was no significant difference (P > 0.05) in tilt angle among 3 groups after 1 and 2 weeks, and there were significant differences (P < 0.05) among 3 groups after 4 weeks. HE staining showed that significant cavity could be seen in group A, little in group B, and no cavity in group C. BrdU immunohistochemistry staining showed that the number of positive cells was 0, 90.54 +/- 6.23, and 121.22 +/- 7.54 in groups A, B, and C, respectively; showing significant differences (P < 0.01) among 3 groups. HRP retrograde neural tracing observation showed that the number of HRP positive nerve fibers was 10.35 +/- 1.72, 43.25 +/- 2.65, and 84.37 +/- 4.59 in groups A, B, and C, respectively, showing significant differences (P < 0.01) among 3 groups. TEM observation showed that a great amount of unmyelinated nerve fibers and myelinated nerve fibers were found in central transverse plane in group C. The BMSCs transplantation play an important role in promotion of recovering the function of hind limb after SCI, and mild hypothermia has synergism effects.

Lei X.-F.,Tianjin Fourth Central Hospital | Zhao P.,Taiyuan Iron and Steel Group Co. General Hospital | Niu Z.-P.,Shanxi Medical University | Cheng Y.,Tianjin Medical University
Chinese Journal of Contemporary Neurology and Neurosurgery | Year: 2014

Objective: To observe the effect of Yangxueqingnao granules on c-Fos protein expression in trigeminal nucleus caudalis (TNC) of nitroglycerin-induced migraine rats.Methods: A total of 108 healthy male Wistar rats were randomly divided into normal control group, model group, high-dose treatment group, low-dose treatment group, high-dose prevention and low-dose prevention group, and then each group was randomly divided into 3 subgroups: 30, 60 and 180 min group. The experimental migraine rat model was established by subcutaneous injection of nitroglycerin. The rats of normal control group were subcutaneously injected with saline solution of the same dosage. The rats of high-and low-dose treatment group were respectively administered with gastric perfusion of high-and low-dose Yangxueqingnao granules once 10 min after the modeling. The rats of high-and low-dose prevention group were respectively administered with gastric perfusion of high-and low-dose Yangxueqingnao granules for 7 d before the modeling. The rats were sacrificed and the brain tissues were collected at corresponding time points. Related behavior indexes of rats in each group were observed and recorded. TNC c-Fos protein expression changes were measured and compared among different groups.Results: At all time points, the c-Fos expression level of model group had no statistical difference from high-and low-dose treatment group (P > 0.05, for all). However, it was higher than that in high-dose prevention group (P = 0.031, 0.000, 0.000), while was only lower than that in low-dose prevention group at 60 and 180 min after modeling (P = 0.000, for all). There was statistical difference between high-and low-dose prevention group only at 30 min after modeling (P = 0.029).Conclusions: The Yangxueqingnao granules can significantly decrease TNC c-Fos protein expression, which suggests that the preventive effect of Yangxueqingnao granules may be greater than the therapeutical effect on migraine. Its preventive effect may be associated with the decrease of TNC c-Fos protein expression.

Xu Z.,Tianjin Fourth Central Hospital | Li G.,Center for Cancer Genomic and Center for Human Genomic
Indian Journal of Otolaryngology and Head and Neck Surgery | Year: 2014

To study the gender differences of amount of neurons in the nodose ganglions of rats. Fourteen Sprague-Dawley rats (7 males and 7 females) were selected. Bilateral nodose ganglions were dissected and serial sections of nodose ganglion were cut in a cryostat, followed by Cresyl-violet staining for neurons. Eight to ten consecutive sections from mid-portion of each nodose ganglion sample, which represent the most neuron number per section, were counted and averaged. Gender difference in the amount of neurons in the nodose ganglions was compared. No gender difference of neuron numbers was found in either side of nodose ganglion (p > 0.05). However, average neuron number of nodose ganglions on the left side of male (654 ± 60) and female (616 ± 37) were significantly more than that on the right side of male (470 ± 22) and female (453 ± 40) respectively (p < 0.05). There is no gender difference in total neuron number of nodose ganglions between male and female rat. However, the neuron number in the left nodose ganglion is greater than that in the right one. The difference may be due to the fact that left and right nodose ganglion is receiving different visceral sensory impulses separately, which is associated with different physiological functions. Further work should be carried out with retrograde tracing on neurons of nodose ganglions in an animal model, which are directly related to laryngeal sensory transmission, in order to determine the gender difference in the neuron number and morphology related to laryngeal functions. © 2012 Association of Otolaryngologists of India.

Yin H.-Q.,Fuxin Central Hospital | Xu L.,4th Peoples Hospital of Shenyang City | Yin L.,Tianjin Fourth Central Hospital
International Journal of Ophthalmology | Year: 2010

• AIM: To investigate the efficacy of photodynamic therapy (PDT) and injection of triamcinolone acetonide (TA) via posterior sub-tenon combination therapy on choroidal neovascularization (CNV) combined with multifocal choroiditis (MC). • METHODS: Eight patients (8 eyes) who were diagnosed as MC combined with CNV were retrospectively analyzed. The changes in the best-corrected visual acuity (BCVA), FFA/ICGA and OCT were analyzed before and after combination therapy. • RESULTS: During 6-month follow-up the BCVA was improved after combination therapy (P < 0.05). FFA/ ICGA showed decrease and stoppage of inflammation on preocular and fundus in 8 eyes (100%), no leakage or reduced leakage in 7 eyes (88%) 1 month after combination therapy. In 1 eye (12%), the fundus remained leaky. At the 6-month follow-up, no inflammation relapse was obtained, FFA / ICGA showed no leakage or slight leakage in 8 eyes (100%). • CONCLUSION: Combination therapy of PDT and injection of TA via posterior sub-tenon is safe and effective to CNV combined with MC.

Luo Z.K.,Tianjin Fourth Central Hospital
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi | Year: 2012

To observe the clinical efficacy and safety of Puerarin Injection treatment on angina pectoris. 388 patients with angina pectoris, enrolled to Tianjin Fourth Central Hospital during January 2009 and February 2011 were selected and randomly divided into treatment or control groups with 194 patients of each. Based on the conventional therapy program, one group was given Puerarin Injection as treatment group while, the other was given with Danshen Injection as control group. Clinical efficacy, the attack rate of angina pectoris, oxygen consumption, indices on electrocardiogram, haemorheology and other adverse reactions among the two groups were compared. The total efficacy of the treatment group (88.14%, 171/194) was significantly higher than the control group (61.86%, 120/194) and the difference was statistically significant (P < 0.05). During the treatment, no significant adverse events were noticed in both of the two groups of patients. The Puerarin Injection treatment program on angina pectoris seemed effective and safe.

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