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Anitua E.,Eduardo Anitua Foundation | Anitua E.,BTI Biotechnology Institute | Zalduendo M.M.,BTI Biotechnology Institute | Alkhraisat M.H.,BTI Biotechnology Institute | Orive G.,BTI Biotechnology Institute
Annals of Anatomy | Year: 2013

Many studies have evaluated the biological effects of platelet rich plasma reporting the final outcomes on cell and tissues. However, few studies have dealt with the kinetics of growth factor delivery by plasma rich in growth factors. Venous blood was obtained from three healthy volunteers and processed with PRGF-Endoret technology to prepare autologous plasma rich in growth factors. The gel-like fibrin scaffolds were then incubated in triplicate, in a cell culture medium to monitor the release of PDGF-AB, VEGF, HGF and IGF-I during 8 days of incubation. A leukocyte-platelet rich plasma was prepared employing the same technology and the concentrations of growth factors and interleukin-1β were determined after 24. h of incubation. After each period, the medium was collected, fibrin clot was destroyed and the supernatants were stored at -80. °C until analysis. The growth factor delivery is diffusion controlled with a rapid initial release by 30% of the bioactive content after 1. h of incubation and a steady state release when almost 70% of the growth factor content has been delivered. Autologous fibrin matrix retained almost 30% of the amount of the growth factors after 8 days of incubation. The addition of leukocytes to the formula of platelet rich plasma did not increase the concentration of the growth factors, while it drastically increased the presence of pro-inflammatory IL-1β. Further studies employing an in vitro inflammatory model would be interesting to study the difference in growth factors and pro-inflammatory cytokines between leukocyte-free and leukocyte-rich platelet rich plasma. © 2013 Elsevier GmbH.

Anitua E.,Eduardo Anitua Foundation
Journal of Craniofacial Surgery | Year: 2015

ABSTRACT: The rehabilitation of edentulous posterior maxilla is a challenging task that could be complicated by the availability of low-density and reduced height of alveolar bone. Patients were enrolled in this study upon the presence of type IV bone and residual bone height <5?mm in posterior maxilla. Novel procedure for transalveolar sinus floor elevation was described to insert 10 short implants in 4 patients. Neither implant failure nor prosthetic failure was observed during a follow-up of 44?±?3 months. Mesial bone loss of 0.5?±?0.6?mm and a distal bone loss 1.3?±?0.9?mm were measured after 35?±?4 months of loading. © 2015 by Mutaz B. Habal, MD.

Anitua E.,Private Practice | Alkhraisat M.H.,Eduardo Anitua Foundation | Pinas L.,Eduardo Anitua Foundation | Pinas L.,Private Practice | Orive G.,Private Practice
Annals of Anatomy | Year: 2015

The primary stability of dental implants is essentially influenced by the quality and quantity of hosting bone. To study the effects of adaptation of the drilling protocol to the biological quality of bone estimated by bone density and cortical/cancellous bone ratio, 8.5. mm-short implants were placed in different bone types by adapting the drilling protocol to result in a socket under-preparation by 0.2, 0.4, 0.7, 1 and 1.2. mm in bone types I, II, III, IV and V, respectively. The effect of the drilling protocol was studied on implant insertion torque and osseointegration. Additionally, we analyzed the relationship of demographic data and social habits to bone type and insertion torque. Then the correlation between insertion torque and bone quality was tested. One hundred ninety two patients (mean age: 62 ± 11 years) participated with 295 implants. The most common bone type at implant site was type III (47.1%) followed by type II (28.1%). Data analysis indicated that gender, age, and social habits had neither correlation with bone type nor with insertion torque. The insertion torque was 59.29 ± 7.27. Ncm for bone type I, 56.51 ± 1.62. Ncm for bone type II, 46.40 ± 1.60. Ncm for bone type III, 34.84 ± 2.38. Ncm for bone type IV and 5. Ncm for bone type V. Statistically significant correlation was found between bone type and insertion torque. The followed drilling protocol adapts socket under-preparation to the needs of establishing a sufficient primary stability for implant osseointegration. © 2014 Elsevier GmbH.

Anitua E.,Private Practice | Troya M.,Eduardo Anitua Foundation | Zalduendo M.M.,Eduardo Anitua Foundation | Orive G.,Eduardo Anitua Foundation
Annals of Anatomy | Year: 2014

Chronic diseases are the major contributors to the global burden of disease and involve prodigious consumption of various drugs that usually affect platelet function. The autologous technology of plasma rich in growth factors (PRGF) provides a biological approach using autologous platelets as a reservoir and local delivery of proteins to promote tissue healing. The purpose of this study was to evaluate the effect of the consumption of acetylsalicylic acid, acenocoumarol and glucosamine sulfate on the preparation as well as on the biological properties of the PRGF technology. Clotting time and platelet activation of PRGF was evaluated. The latter was performed by flow cytometry. PRGF growth factor content and the release of various biomolecules by gingival fibroblasts were quantified by enzyme-linked immunosorbent assay. Cell proliferation was evaluated by means of a fluorescence-based method and cell migration was performed on culture inserts. None of the parameters evaluated was modified by the consumption of any of the three drugs tested; only the plasma of patients who had consumed acetylsalicylic acid and acenocoumarol expressed greater gingival fibroblast migration compared to plasma control. The intake of acetylsalicylic acid, acenocoumarol and glucosamine sulfate does not alter the preparation and biological properties of the autologous technology of PRGF. © 2014 Elsevier GmbH.

Duran-Cantolla J.,Sleep Disorders Unit | Duran-Cantolla J.,Hospital Universitario La Paz | Duran-Cantolla J.,Research Center Biomedica en Red Enfermedades Respiratorias | Duran-Cantolla J.,University of the Basque Country | And 7 more authors.
Journal of Clinical Sleep Medicine | Year: 2015

Study Objectives: To estimate the frequency of obstructive sleep apnea syndrome (OSAS) in dental patients with tooth wear, and to assess the role of dentists in the identification of patients at risk of OSAS. Methods: Dental patients with tooth wear and treated with occlusal splint were prospectively recruited to perform sleep study. The severity of tooth wear was established by the treating dentist before patient referral to sleep disorders unit. Sleep questionnaires, anthropometric measurements, and validated respiratory polygraphy were performed. Results: All patients with dental wear were offered a sleepiness analysis. Of 31 recruited patients, 30 (77% males) participated in this study. Patients' mean age was 58.5 ± 10.7 years (range: 35-90 years) and the body mass index was 27.9 ± 3.4 kg/m2. Tooth wear was mild in 13 patients, moderate in 8 and severe in 9. The mean apnea-hypopnea index (AHI) was 32.4 ± 24.9. AHI < 5 was reported in 2 patients, AHI of 5-29 in 17, and AHI ≥ 30 in 11. A statistically significant association was found between AHI severity and tooth wear severity (Spearman R = 0.505; p = 0.004). Conclusions: Tooth wear could be a tool to identify those patients at risk of having OSAS. This highlights the importance of dental professionals to identify and refer patients with OSAS. © 2015, American Academy of Sleep Medicine. All rights reserved.

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