Foshan Institute of Occupational Disease Prevention and Control

Foshan, China

Foshan Institute of Occupational Disease Prevention and Control

Foshan, China
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Gu C.,U.S. Center for Disease Control and Prevention | Gu C.,Sun Yat Sen University | Li X.,Guangdong Prevention and Treatment Center for Occupational Diseases | Tan Q.,Foshan Institute of Occupational Disease Prevention and Control | And 4 more authors.
Toxicology in Vitro | Year: 2012

The roles of miRNAs in the onset of hearing and deafness are beginning to be revealed. Although there has been no reported link between chloride intracellular channel 5 (CLIC5) and the miR-183 family to date, we here present evidence that they are co-expressed in the inner ear and have functions that are related to stereocilia. Moreover, CLIC5 contains a single predicted and highly conserved miR-96/-182 binding site within its 3′-UTR. Our current results further show that miR-96/-182 and CLIC5 are co-expressed in HEI-OC1 cells, in which two isoforms of the CLIC5 protein exist. Furthermore, miR-96 and miR-182 were found to be specifically overexpressed in HEI-OC1 cells into which mimics of these molecules had been transfected by liposomes causing the downregulation of CLIC5 at both the mRNA and protein levels. Finally, miR-96/-182 specifically downregulate the expression of the luciferase reporter gene which was cloned into a mouse CLIC5 3′-UTR fragment containing the wild-type miR-96/-182 target sequence. Our findings thus suggest that CLIC5 is directly regulated by miR-96 and miR-182 and that the target sequence in this regard is located between nucleotides 760-766 within the CLIC5 3′-UTR. © 2012 Elsevier Ltd. All rights reserved.


Chen G.-L.,Foshan Institute of Occupational Disease Prevention and Control | Chen S.-G.,Foshan Institute of Occupational Disease Prevention and Control | Xie Y.-Q.,Foshan Institute of Occupational Disease Prevention and Control | Chen F.,Foshan Institute of Occupational Disease Prevention and Control | And 3 more authors.
Journal of Functional Foods | Year: 2015

In order to provide new information on the antioxidant activities before and after in vitro digestion of the selected flowers for consumers, nutritionists, and food policy makers, total phenolic and flavonoid contents and antioxidant capacities of 23 edible flowers were evaluated. In addition, some bioactive components in the flowers were also identified and quantified by ultra performance liquid chromatography (UPLC). The correlations between 2, 2-diphenyl-1-picrylhydrazylradical scavenging activity assay (DPPH), Ferric reducing antioxidant power assay (FRAP), and ABTS•+ radical cation scavenging activity assay (ABTS) values and total flavonoid contents and total phenolic contents were also evaluated. Three flowers, Osmanthus fragrans (Thunb.) Lour, Paeonia lactiflora Pall, and Rosa rugosa Thunb (purple) showed the strongest antioxidant activities before or after the digestion, which implied that these flowers are important natural sources for the prevention of oxidative stress diseases. © 2015 Elsevier Ltd.


Liu L.,Peking University | Chen S.G.,Foshan Institute of Occupational Disease Prevention and Control | Tang S.C.,Beijing Municipal Institute of Labour Protection | Wang S.,Peking University | And 5 more authors.
Biomedical and Environmental Sciences | Year: 2015

Objective In this study, we aimed at exploring the association between work-related musculoskeletal disorders (WMSDs) and work organization based on a case-control study. Methods A total of 1938 workers who claimed to suffer from WMSDs were selected from Beijing, Henan, Hubei, and the Guangdong province. The control group consisted of 2009 workers employed in similar industries without severe disease or musculoskeletal discomforts. We used a modified version of the questionnaire developed by the NMQ and the DMQ to investigate individual and work-related factors. Results A total of 13 variables (P<0.1) were selected by the chi-square test and finally, 7 variables entered into the equation, with 6 variables reaching statistical significance (P<0.05). The odds ratios (OR) of 'work changing with season' and 'sufficient rest time' did not reach 1 (0.749 and 0.441, respectively). In addition, 'sufficient rest time' seemed to be the stronger protective factor according to its higher standardized coefficient. And 'repetitive work every minute', 'constantly repetitive work' (every day), 'shortage of site personnel', and 'often switching shifts with others' seemed to be the risk factors. Conclusion Work organization may have comprehensive effects on the occurrence of WMSDs. This pattern of associations suggests that further investigation into the mechanism of how work organization affects the prevalence of WMSDs is required. © 2015 The Editorial Board of Biomedical and Environmental Sciences.


Wang Y.-K.,Guangdong Pharmaceutical University | Zhang X.,China Institute of Technology | Chen G.-L.,Foshan Institute of Occupational Disease Prevention and Control | Yu J.,Guangdong Pharmaceutical University | And 2 more authors.
Journal of Functional Foods | Year: 2016

To explore the potential of common beans as natural sources of bioactive compounds, the antioxidant properties and total phenolic content (free, soluble conjugate, insoluble-bound fraction) of 14 beans from China were evaluated systematically. The results showed that beans had diverse antioxidant capacities and the variation was very large. Black bean, flower waist bean, pearl bean and spring bay bean showed the strongest antioxidant activities among the 14 tested samples based on a combinative consideration of the results obtained by 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH) assay, ferric reducing antioxidant power (FRAP) assay, and trolox equivalent antioxidant capacity (TEAC) assay. Furthermore, several phenolic compounds were detected; gallic acid and protocatechuic acid were widely found in these beans. The results implied that these beans were important natural sources for preventing oxidative stress diseases. © 2016 Elsevier Ltd.


Chen G.-L.,Foshan Institute of Occupational Disease Prevention and Control | Chen S.-G.,Foshan Institute of Occupational Disease Prevention and Control | Chen F.,Foshan Institute of Occupational Disease Prevention and Control | Xie Y.-Q.,Foshan Institute of Occupational Disease Prevention and Control | And 4 more authors.
Journal of Functional Foods | Year: 2016

In order to find new sources of natural antioxidants, total phenolic contents and antioxidant capacities of 11 fruit seeds before and after in vitro digestion were evaluated by Folin-Ciocalteu method, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity assay (DPPH), ferric reducing antioxidant power assay (FRAP), and ABTS•+ scavenging activity (ABTS) assay, respectively. The correlations between DPPH, FRAP, ABTS values and total phenolic contents were also evaluated. The results showed that 11 fruit seeds had diverse antioxidant capacities and the variation was very large. Furthermore, the main compounds of 11 fruit seeds were identified and quantified. Four fruit seeds, Dimocarpus longgana Lour, Vitis vinifera Linn (red), Vitis vinifera Linn (black), and Litchi chinensis Sonn showed the strongest antioxidant activities whether before or after digestion, which implied that these fruit seeds are important natural sources for preventing oxidative stress diseases. © 2015 Elsevier Ltd.


PubMed | Henan Provincial Institute for Occupational Health, Institute for Occupational Health and Poison Control, Foshan Institute of Occupational Disease Prevention and Control, Peking University and 2 more.
Type: Journal Article | Journal: Biomedical and environmental sciences : BES | Year: 2015

In this study, we aimed at exploring the association between work-related musculoskeletal disorders (WMSDs) and work organization based on a case-control study.A total of 1938 workers who claimed to suffer from WMSDs were selected from Beijing, Henan, Hubei, and the Guangdong province. The control group consisted of 2009 workers employed in similar industries without severe disease or musculoskeletal discomforts. We used a modified version of the questionnaire developed by the NMQ and the DMQ to investigate individual and work-related factors.A total of 13 variables (P<0.1) were selected by the chi-square test and finally, 7 variables entered into the equation, with 6 variables reaching statistical significance (P<0.05). The odds ratios (OR) of work changing with season and sufficient rest time did not reach 1 (0.749 and 0.441, respectively). In addition, sufficient rest time seemed to be the stronger protective factor according to its higher standardized coefficient. And repetitive work every minute, constantly repetitive work (every day), shortage of site personnel, and often switching shifts with others seemed to be the risk factors.Work organization may have comprehensive effects on the occurrence of WMSDs. This pattern of associations suggests that further investigation into the mechanism of how work organization affects the prevalence of WMSDs is required.


PubMed | Foshan Institute of Occupational Disease Prevention and Control, Guangdong Medical University, Guangzhou University, Southern Medical University and Sun Yat Sen University
Type: Journal Article | Journal: Molecular carcinogenesis | Year: 2016

Hydroquinone (HQ), a metabolite of benzene, is a well-known human carcinogen; however, its molecular mechanisms of action remain unclear. MeCP2 has been traditionally described as a transcriptional repressor, though growing evidence indicates that it also activates gene expression. Here, we investigated whether some epigenetic machinery genes are aberrantly expressed as target tumor suppressor genes in HQ-transformed TK6 lymphoblastoid cells. Our results showed that treatment with 5-Aza-2-deoxycytidine or trichostatin A enhanced the expression of Rb, resulting in cell arrest in G1-phase, and subsequently, an increase in apoptosis and a decrease in cell growth. Moreover, we hypothesised that Rb was silenced by the down-regulation of MeCP2 in HQ-transformed cells, resulting in the dynamic expression of Rb and epigenetic machinery proteins in HQ-transformed cells at different time points. The expression of Rb and MeCP2 in patients with B-cell non-Hodgkins lymphoma (B-NHL) showed that positive staining for MeCP2 or Rb was significantly lower in B-NHL tumor tissues, and these changes were significantly and negatively correlated with the grade of B-NHL. The restoration of MeCP2 in HQ-transformed cells enhanced the expression of Rb, promoted cell apoptosis, and inhibited cell growth. The changes in the expression patterns of MeCP2 and Rb were inversely correlated with the degree of DNA methylation. A ChiP assay revealed that MeCP2 proteins were recruited to the Rb promoter with lower 5-methylcytosine levels. In conclusion, we demonstrated that the down-regulation of MeCP2 silences Rb, a process involved in cell transformation resulting from long-term exposure to HQ. 2016 Wiley Periodicals, Inc.

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