Foshan, China
Foshan, China

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Wu W.-J.,Foshan Blood Center | Guo R.-H.,Foshan Blood Center | Yu J.-L.,Foshan Blood Center
Chinese Journal of Biologicals | Year: 2010

Objective: To analyze the genotype and genetic background of a Rh D - individual and her family members. Methods: The specific sequences of RHD and RHCE genes of a Rh D - individual and her family members were amplified by PCR-SSP and analyzed. Results: The PCR results of family members of the index case were basically consistent with their serological phenotypes. The RHD gene of index case showed no abnormality, while no specific sequence of RHCE gene was detected. However, the phenotypes and PCR products of RHCE genes of her family members were normal. Conclusion: The important molecular basis of Rh D - was partial or complete deletion of RHCE gene of index case, which was deduced to be due to the two D - haplotypes carried by her father and mother respectively.


Cai X.-Y.,Foshan Blood Center
Chinese Journal of Biologicals | Year: 2012

Objective: To develop two noninvasive methods and provide a reference for accurate estimation of volume of red blood cell components. Methods: The net weights and volumes of 130 bags of red blood cell components were measured, based on which the mean blood density was calculated. The correlation between the net weight and volume of red blood cell components was analyzed and assessed by a linear regression model, based on which a regression equation was established. Another equation for calculation of blood volume was established based on the mean density and net weight of blood. One hundred bags of red blood cell components were tested and analyzed by the two equations, of which the volumes were calculated from net weight and used as the expected values. The difference between practically measured volumes and the expected values was analyzed by t test to verify the reliability of the method. Results: The net weights of 130 bags of red blood cell components were positively related to their volumes (r = 0.998, P < 0.01), of which the linear regression equation was y = 0.942 3 x-2.394 1. The mean density of the 130 bags of red blood cell components was 1.072 8, of which the equation for calculation was y = 0. 932 2 x. No significant difference was observed between the practically measured volumes and the expected values of 100 bags of red blood cell components(P > 0.05). Conclusion: Two methods for estimation of volume of red blood cell components were developed, which were simple, safe and reliable, and were worthy of popularization in transfusion.


Zhu Y.-H.,Foshan Blood Center | Wu W.-J.,Foshan Blood Center | Ma C.-H.,Foshan Blood Center | Guo R.-H.,Foshan Blood Center | Yu J.-L.,Foshan Blood Center
Chinese Journal of Biologicals | Year: 2012

Objective: To analyze the relationship between blood group antibody IgG titer of pregnant women and ABO hemolytic disease of newborn (HDN). Methods: Three hundreds of pregnant women in Foshan City as well as their husbands were subjected to ABO blood grouping, while the pregnant women were determined for anti-A and/or anti-B IgG titers in sera. From the newborns with clinical indications of HDN, the blood samples were collected for ABO blood grouping as well as Coomb test, free antibody test and antibody release test. The relationship between blood group antibody IgG titer of pregnant women and HDN in ten provinces and cities including Foshan City in China was analyzed. Results: All the 300 pregnant women in Foshan City were of blood group O, while their the husbands were of blood groups A, B or AB. In all the ten provinces and cities, the incidence rates of HDN increased with the increasing blood antibody IgG titers of pregnant women. However, the incidence rate showed significant difference in the newborn delivered by pregnant women with the same IgG titers in various provinces and cities(each P < 0.05) and in the newborns delivered by pregnant women with various IgG titers (each P < 0.05). The blood group antibody IgG titers of pregnant women was positively related to the incidence rate of HDN (r = 0.866, P < 0.05). The increasing fold of incidence rate of HDN in newborns delivered by pregnant women with IgG titers of 32-64 was 440%, which was higher than those by pregnant women with other IgG titers. Conclusion: The blood group IgG titer of pregnant woman might be used as one of the indexes, but not the only index, for evaluation of risk of HDN. The risk should be evaluated by the IgG titer combined with other test results and clinical status of pregnant women.

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