Brown J.L.,Conservation and Research Center |
Kersey D.C.,Conservation and Research Center |
Freeman E.W.,Conservation and Research Center |
Freeman E.W.,George Mason University |
Wagener T.,Fort Worth Zoo
Zoo Biology | Year: 2010
Longitudinal urine samples were collected from Asian and African elephants to assess sample processing and immunoassay techniques for monitoring adrenal activity. Temporal profiles of urinary cortisol measured by RIA and EIA, with and without dichloromethane extraction, were similar; all correlation coefficients were >0.90. However, based on regression analyses, cortisol immunoactivity in extracted samples was only 72-81% of that of unextracted values. Within assay technique, RIA values were only 74-81% of EIA values. Collection of 24-hr urine samples demonstrated a clear diurnal pattern of glucocorticoid excretion, with the lowest concentrations observed just before midnight and peak concentrations occurring around 0600-0800 hr. These results indicate that elephants fit the pattern of a diurnal species, and that glucocorticoid production is affected by a sleep-wake cycle similar to that described for other terrestrial mammals. Cortisol can be measured in both extracted and unextracted urine using RIA and EIA methodologies. However, unexplained differences in quantitative results suggest there may be sample matrix effects and that data generated using different techniques may not be directly comparable or interchangeable. © 2009 Wiley-Liss, Inc.
Stoops M.A.,Center for Conservation and Research of Endangered Wildlife |
West G.D.,Oklahoma City Zoo |
Roth T.L.,Center for Conservation and Research of Endangered Wildlife |
Lung N.P.,Fort Worth Zoo
Zoo Biology | Year: 2014
Urinary hormone analysis was conducted on two adult female Indian rhinoceroses (Rhinoceros unicornis) that exhibited minimal or no estrual behaviors traditionally used to time breeding. Urine was collected throughout two consecutive estrous cycles to establish preliminary data on each individual's pattern and concentration of estrogen conjugates (EC) and progesterone metabolites (PdG) during follicular and luteal phases. Following preliminary endocrine analysis, urine samples were shipped on a frequent basis to verify when each female was off baseline in EC. Estrus and breeding dates were then predicted. Females were introduced to fresh male rhinoceros fecal samples daily throughout the follicular phase to potentially stimulate estrous behaviors. Despite successful assessment of follicular phase dynamics, females sometimes failed to exhibit estrus. Both females conceived following mating introductions that were timed using hormone analysis. Pregnancy was diagnosed either by endocrine analysis or rectal ultrasonography. Progestational support (altrenogest) occurred after pregnancy confirmation and varied for each female (21 and 66 days post-breeding). One female experienced early pregnancy loss and the other successfully completed a term pregnancy. These results demonstrate that a science based management strategy that relies on urinary biomarkers of ovarian function can facilitate naturally breeding captive Indian rhinoceroses. © 2013 Wiley Periodicals, Inc.
Williams D.A.,Texas Christian University |
Leach C.,Texas Christian University |
Hale A.M.,Texas Christian University |
Karsten K.B.,Texas Christian University |
And 5 more authors.
Conservation Genetics Resources | Year: 2012
We developed a non-invasive DNA sampling method and 15 tetranucleotide microsatellite markers for Texas horned lizards (Phrynosoma cornutum). Swabbing the cloaca with a small cotton swab and preserving the cells in lysis buffer was an effective method to obtain tissue for DNA extraction. Loci were highly polymorphic with 8-25 alleles and observed heterozygosity was high (0.71-0.96). Some of these loci can also be used for round-tailed horned lizards (P. modestum). © Springer Science+Business Media B.V. 2011.
News Article | March 21, 2016
The Thursday-morning hailstorms last week that pounded cars and rolled across North Texas could lead to $300 million in vehicle repairs alone. Mark Hanna, a spokesperson for the Insurance Council of Texas, said that an estimated 50,000 vehicles were battered by the storms from Fort Worth to Arlington. “This is a catastrophic event for us,” Hanna says in a Forth Worth Star-Telegram report of the hail, which fell in two occasions and varied from the size of blueberries to tennis balls. The number of damaged roofs could be around one-third of the number of damaged vehicles, Hanna added, although the cost of their repair could be double that of the vehicles’. Reports have it that insurance firm State Farm already received at least 3,850 car claims and 1,480 home claims. Last Sunday, it opened its vehicle assess sites to address the claims, and will open three more by the week’s end. The storms left the streets with debris, leaves, and some remnants of damaged structures. Even the West Division headquarters of Fort Worth Police Department was not spared, just like the Fort Worth Zoo where eight birds and five flamingos were killed. But there were also emerging winners from the damage, such as companies like Auto Glass Now – located in west Fort Worth off Interstate 30 – that already provided hundreds of estimates for affected windows. The weekend had also been likely been a lucrative one for AAA Glass, which worked on cars or replaced home windows. Hail occurs when updrafts in thunderstorms become carriers of raindrops upward into very cold areas where they freeze into ice. Hail can damage not only structures but also livestock and crops. Research focuses on accurate prediction and detection of hailstorms to give those who run in the path of the storm enough preparation time to seek shelter and protect their personal property.
Eight exotic birds were killed by hail which also damaged the canopy of a carousel at the Fort Worth Zoo, as shown in this handout photo taken in Fort Worth, Texas on March 17, 2016, and provided by the Fort Worth Zoo. Hail that killed eight exotic birds and also caused damage at the Fort Worth Zoo is shown in this handout photo taken in Fort Worth, Texas on March 17, 2016, and provided by the Fort Worth Zoo. Eight exotic birds were killed by hail which also damaged the canopy of a carousel at the Fort Worth Zoo as shown in this handout photo taken in Fort Worth, Texas on March 17, 2016, and provided by the Fort Worth Zoo. Eight exotic birds were killed at the Fort Worth Zoo on Thursday by a violent hail storm that dropped ice pellets the size of golf balls, a zoo spokeswoman said. Among the birds killed were five flamingos, a pelican, an ibis and a black-necked swan chick, said Alexis Wilson, the zoo's director of communications. The hail that hit at around 6:30 a.m. also damaged roofs and skylights, she said, but almost all of the other residents at the 7,000-animal zoo were unaffected by the hail.