Haupt K.,Max Planck Research Unit for Enzymology of Protein Folding |
Haupt K.,Forschungsinstitut For Leder Und Kunststoffbahnen |
Jahreis G.,Max Planck Research Unit for Enzymology of Protein Folding |
Linnert M.,Max Planck Research Unit for Enzymology of Protein Folding |
And 8 more authors.
Journal of Biological Chemistry | Year: 2012
FKBP38 is a regulator of the prosurvival protein Bcl-2, but in the absence of detailed structural insights, the molecular mechanism of the underlying interaction has remained unknown. Here, we report the contact regions between Bcl-2 and the catalytic domain of FKBP38 derived by heteronuclear NMR spectroscopy. The data reveal that a previously identified charge-sensitive loop near the putative active site of FKBP38 is mainly responsible for Bcl-2 binding. The corresponding binding epitope of Bcl-2 could be identified via a peptide library-based membrane assay. Site-directed mutagenesis of the key residues verified the contact sites of this electrostatic protein/protein interaction. The derived structure model of the complex between Bcl-2 and the FKBP38 catalytic domain features both electrostatic and hydrophobic intermolecular contacts and provides a rationale for the regulation of the FKBP38/Bcl-2 interaction by Ca2+. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.
Schmalzm E.,Sachsiches Textilforschungsinstitut E V |
Heidenreich R.,Institute For Luft Und Kaltetechnik Gemeinnutzige Gesellschaft Mbh |
Rudolph H.,Forschungsinstitut For Leder Und Kunststoffbahnen
Kettenwirk-Praxis | Year: 2011
The use of biomasses in small incineration plants for the purposes of heating will increase in the future. Emissions from incineration plants must be decreased in order to comply with the requirements of environmental protection. As far as total emissions from boiler plants are concerned, it is also important for the filter system to have a low resistance and thus to influence the pressure conditions in the boiler. In this case, new filter materials have to be developed. The current research work has enabled the requirements relating to the air permeability to be met. Extensive investigations were carried out into the chemical resistance of the fibres, and the best materials were selected on the basis of the results. The filtration studies carried out on filter media based on pile-fibre stitch-bonded nonwovens resulted in low pressure differences for very different filtration performances. The majority of the textile materials did not achieve the target values for dust concentration levels in the clean gas. However, the results do give an indication of the possibilities and the direction that the development work should take. One of the filter materials produced has already achieved a filtration efficiency of above 95%.
Rudek F.,Zwickau University of Applied Sciences |
Nelsen B.L.,Zwickau University of Applied Sciences |
Baselt T.,Zwickau University of Applied Sciences |
Baselt T.,Fraunhofer Institute for Material and Beam Technology |
And 5 more authors.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE | Year: 2016
Nutrient and oxygen transport, as well as the removal of metabolic waste are essential processes to support and maintain viable tissue. Current bioreactor technology used to grow tissue cultures in vitro has a fundamental limit to the thickness of tissues. Based on the low diffusion limit of oxygen a maximum tissue thickness of 200 ìm is possible. The efficiency of those systems is currently under investigation. During the cultivation process of the artificial tissue in bioreactors, which lasts 28 days or longer, there are no possibilities to investigate the viability of cells. This work is designed to determine the influence of a non-disruptive cell viability measuring system on cellular activity. The measuring system uses a natural cellular marker produced during normal metabolic activity. Nicotinamide adenine dinucleotide (NADH) is a coenzyme naturally consumed and produced during cellular metabolic processes and has thoroughly been studied to determine the metabolic state of a cell. Measuring the fluorescence of NADH within the cell represents a non-disruptive marker for cell viability. Since the measurement process is optical in nature, NADH fluorescence also provides a pathway for sampling at different measurement depths within a given tissue sample. The measurement system we are using utilizes a special UV light source, to excite the NADH fluorescence state. However, the high energy potentially alters or harms the cells. To investigate the influence of the excitation signal, the cells were irradiated with a laser operating at a wavelength of 355 nm and examined for cytotoxic effects. The aim of this study was to develop a non-cytotoxic system that is applicable for large-scale operations during drug-tissue interaction testing. © 2016 SPIE.
Schulz B.,Forschungsinstitut For Leder Und Kunststoffbahnen |
Meinert T.,RWTH Aachen |
Bierbusse D.,RWTH Aachen |
Busen M.,RWTH Aachen |
And 3 more authors.
Chemie-Ingenieur-Technik | Year: 2016
Aerogels are high-performance materials used for space and aviation purposes. Cellulose aerogel fibers have been investigated under real space conditions for their insulation properties. The experiments were carried out in a one-stage high-altitude research rocket of the improved ORION type. A cuboid module with measurement cells, camera modules, and electronic devices has been developed for monitoring the insulation behavior of cellulose aerogel non-woven samples. The thermal behavior of these samples has been analyzed and compared to cellulose cloth (cotton) and aluminum for reference. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Meyer M.,Forschungsinstitut For Leder Und Kunststoffbahnen |
Prade I.,Forschungsinstitut For Leder Und Kunststoffbahnen |
Leppchen-Frohlich K.,Forschungsinstitut For Leder Und Kunststoffbahnen |
Felix A.,TU Bergakademie Freiberg |
And 3 more authors.
Journal of Supercritical Fluids | Year: 2015
Collagen sponges and membranes which are used as medical device and in tissue engineering (TE) were sterilised by using supercritical carbon dioxide (CO2sc) which was doted with hydrogen peroxide (300 mg/L). Sterilisation was achieved by pressure change treatment (20.5-10 MPa). Neither physical nor structural and biological parameters of the materials were negatively affected. In contrast, sterilisation by gamma-irradiation led to increased solubility and decreased physical stability. As H2O2 is a common chemical used in collagen processing in industrial scale this could become a sterilisation technique for medical devices and TE materials on collagen basis. © 2015 Elsevier B.V. All rights reserved.