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Chen T.-S.,Chung Yuan Christian University | Liou S.-Y.,Formosan Blood Purification Foundation | Chang Y.-L.,Chung Yuan Christian University
International Urology and Nephrology | Year: 2010

Background The purpose of this study was to evaluate two commercial hemodialyzers (F100 and P21) through the clearance of identified and unidentified uremic solutes. Methods We investigated plasma samples from healthy and uremic subjects. Results We found three unidentified uremic solutes (P1, P2, and P3) after HPLC analysis of plasma samples. Then, we determined the clearance of four identified and the three unidentified uremic solutes in patients with F100 and P21. There was no significance between F100 and P21 in clearance of four identified uremic solutes. By contrast, the clearance of P1 and P2 with F100 was superior with the P21 (P<0.05). Conclusion Our data suggest that selection of a hemodialyzer based on the clearance of identified uremic solutes might not be sufficient. Application of external techniques to determine additional external factors (i.e., clearance of unidentified uremic solutes) can facilitate a better decision in the selection of the ideal hemodialyzer. © Springer Science+Business Media, B.V. 2009. Source


Chen T.-S.,China Medical University at Taichung | Liou S.-Y.,Formosan Blood Purification Foundation | Wu H.-C.,China Medical University at Taichung | Wu M.-L.,Chung Yuan Christian University | And 5 more authors.
Food Chemistry | Year: 2012

Hemodialysis can remove uremic solutes but this treatment induces oxidative stress in uremic patients because of hemo-incompatibility. Therefore, we hypothesised that an antioxidant dialysate (a dialysate containing antioxidant(s)) would provide antioxidant defence in uremic patients during hemodialysis. Several herbal extracts were studied and measurements of antioxidant power and stability assays indicated that epigallocatechin gallate (EGCG) was the best of those tested for use as an antioxidant dialysate (EGCG dialysate). We observed that EGCG dialysate could provide the highest level of antioxidant defence at a dialysate flow rate of 500 ml/min and a blood flow rate of 200 ml/min. In addition, some important parameters for hemodialysis were calculated for supporting the protective role of EGCG dialysate. This is the first description of the preparation of an antioxidant dialysate. We suggest that EGCG dialysate will reduce the level of oxidative stress in hemodialysis patients, leading to a decrease of complications associated with oxidative damage. © 2012 Elsevier Ltd. All rights reserved. Source


Chen T.-S.,China Medical University at Taichung | Liou S.-Y.,Formosan Blood Purification Foundation | Wu H.-C.,China Medical University at Taichung | Tsai F.-J.,China Medical University at Taichung | And 4 more authors.
Food Chemistry | Year: 2012

Polyphenols, such as (-)-epigallocatechin gallate (EGCG), have pro-oxidant properties that lead to activation of the cellular apoptotic pathway; however, the mechanism underlying the induction of this pro-oxidant activity is unclear. We found intensive chemiluminescence for EGCG and hydroquinone (HQ) in the presence of amino acids with basic amino side-chains, such as arginine or lysine. In addition, the chemiluminescence of EGCG and arginine or lysine was totally suppressed by the addition of superoxide dismutase; whereas the chemiluminescence of HQ and arginine or lysine was totally suppressed by the addition of catalase. Furthermore, we found that polyphenols are capable of inducing oxidative stress, including depletion of albumin thiol content, elevation of plasma oxidized low-density lipoprotein and acceleration of cell death in the presence of arginine. Taking the results together, we suggest that amino acids with basic amino side-chain accelerate the production of reactive oxygen species in the presence of polyphenols. © 2012 Elsevier Ltd. All rights reserved. Source


Kuo C.-L.,China Medical University at Taichung | Chen T.-S.,China Medical University at Taichung | Liou S.-Y.,Formosan Blood Purification Foundation | Hsieh C.-C.,China Medical University at Taichung | Hsieh C.-C.,Tunghai University
Immunopharmacology and Immunotoxicology | Year: 2014

Green tea is a widely consumed beverage known for its beneficial anti-inflammatory, anti-oxidative, anti-mutagenic, anti-carcinogenic, and cardioprotective properties. Here, we administered epigallocatechin gallate fraction of green tea extract (EGTE) to mice for 6 weeks and examined the effects on the innate and adaptive immune responses by measuring phagocytic and natural killer (NK) cell activity, as well as antigen-specific proliferation, cytolysis, cytokine secretion, and antibody production. Our data show that EGTE administration increased NK cell cytolysis and peritoneal cell phagocytosis, as well as splenocyte proliferation and secretion of IL-2 and IFN-γ. Of note, EGTE treatment decreased the production antigen-specific IgE via increased the proportion of CD4+ CD25+ regulatory T lymphocytes in the spleen, suggesting that EGTE may play a role in regulating the allergic response. © 2014 Informa Healthcare USA, Inc. All rights reserved. Source


Chen T.-S.,China Medical University at Taichung | Liou S.-Y.,Formosan Blood Purification Foundation | Wu H.-C.,China Medical University at Taichung | Tsai F.-J.,China Medical University at Taichung | And 3 more authors.
Journal of Agricultural and Food Chemistry | Year: 2010

Several analytical approaches are available for investigating the antioxidant power for antioxidants, and they are based on a variety of chemical principles, such as oxygen radical absorbance capacity (ORAC), Trolox equivalent antioxidant capacity (TEAC), and ferric reducing/antioxidant power (FRAP). This paper reports a new rapid method for investigating antioxidant power on the basis of the electron-donating ability. This method is called chemiluminescence analysis of antioxidant power (CAAP). The electrons donated from antioxidants are capable of inducing chemiluminescence in the presence of lucigenin and a base. Thus, the intensity of chemiluminescence induced by antioxidants is proportional to their electron-donating ability (antioxidant power). It was found that the correlation between CAAP and FRAP was positive (r = 0.959) and statistically significant (p < 0.05). In addition to the FRAP assay, the rapid CAAP assay is convenient for investigating the antioxidant power of herbal extracts. © 2010 American Chemical Society. Source

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