Forestry Research Institution of Heilongjiang Province

Harbin, China

Forestry Research Institution of Heilongjiang Province

Harbin, China

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Liu C.-C.,Northeast Forestry University | Liu C.-C.,Beijing Research Institute of Chemical Defense | Li C.-M.,Forestry Research Institution of Heilongjiang Province | Liu B.-G.,Beihua University | And 6 more authors.
Plant Molecular Biology Reporter | Year: 2012

Dehydrins (DHNs) define a complex group of stress inducible proteins characterized by the presence of one or more lysine-rich motifs. DHNs are present in multiple copies in the genome of plant species. Although genome-wide analysis of DHNs composition and chromosomal distribution has been conducted in herbaceous species, it remains unexplored in woody plants. Here, we report on the identification of ten genes encoding eleven putative DHN polypeptides in Populus. We document that DHN genes occur as duplicated blocks distributed over seven of the 19 poplar chromosomes likely as a result of segmental and tandem duplication events. Based on conserved motifs, poplar DHNs were assigned to four subgroups with the K n subgroup being the most frequent. One putative DHN polypeptide (PtrDHN-10) with a SKS arrangement could originate from a recombination between SK n and K nS genes. In silico analysis of microarray data showed that in unstressed poplar, DHN genes are expressed in all vegetative tissues except for mature leaves. This exhaustive survey of DHN genes in poplar provides important information that will assist future studies on their functional role in poplar. © 2011 Springer-Verlag.


Zhu H.-Y.,Northeast Forestry University | Li C.-M.,Forestry Research Institution of Heilongjiang Province | Wang L.-F.,Mudanjiang Normal University | Bai H.,Forestry Research Institution of Heilongjiang Province | And 5 more authors.
International Journal of Molecular Sciences | Year: 2014

N-terminal acetyltransferase (Nats) complex is responsible for protein N-terminal acetylation (Nα-acetylation), which is one of the most common covalent modifications of eukaryotic proteins. Although genome-wide investigation and characterization of Nat catalytic subunits (CS) and auxiliary subunits (AS) have been conducted in yeast and humans they remain unexplored in plants. Here we report on the identification of eleven genes encoding eleven putative Nat CS polypeptides, and five genes encoding five putative Nat AS polypeptides in Populus. We document that the expansion of Nat CS genes occurs as duplicated blocks distributed across 10 of the 19 poplar chromosomes, likely only as a result of segmental duplication events. Based on phylogenetic analysis, poplar Nat CS were assigned to six subgroups, which corresponded well to the Nat CS types (CS of Nat A-F), being consistent with previous reports in humans and yeast. In silico analysis of microarray data showed that in the process of normal development of the poplar, their Nat CS and AS genes are commonly expressed at one relatively low level but share distinct tissue-specific expression patterns. This exhaustive survey of Nat genes in poplar provides important information to assist future studies on their functional role in poplar. © 2014 by the authors; licensee MDPI, Basel, Switzerland.


Liu C.-C.,Northeast Forestry University | Liu C.-C.,Beijing Research Institute of Chemical Defense | Liu B.-G.,Beihua University | Yang Z.-W.,Jiamusi University | And 3 more authors.
International Journal of Molecular Sciences | Year: 2012

Peptide deformylases (PDF) behave as monomeric metal cation hydrolases for the removal of the N-formyl group (Fo). This is an essential step in the N-terminal Met excision (NME) that occurs in these proteins from eukaryotic mitochondria or chloroplasts. Although PDFs have been identified and their structure and function have been characterized in several herbaceous species, it remains as yet unexplored in poplar. Here, we report on the first identification of two genes (PtrPDF1A and PtrPDF1B) respectively encoding two putative PDF polypeptides in Populus trichocarpa by genome-wide investigation. One of them (XP_002300047.1) encoded by PtrPDF1B (XM_002300011.1) was truncated, and then revised into a complete sequence based on its ESTs support with high confidence. We document that the two PDF1s of Populus are evolutionarily divergent, likely as a result of independent duplicated events. Furthermore, in silico simulations demonstrated that PtrPDF1A and PtrPDF1B should act as similar PDF catalytic activities to their corresponding PDF orthologs in Arabidopsis. This result would be value of for further assessment of their biological activities in poplar, and further experiments are now required to confirm them. © 2012 by the authors.


Li C.-M.,Beijing Forestry University | Li C.-M.,Forestry Research Institution of Heilongjiang Province | Wang Y.,Northeast Forestry University | Yu W.-X.,Heilingjiang Academy of Forestry
Journal of Forestry Research | Year: 2011

We investigated seasonal dynamics of phenolics substance in leaf and bark of two cultivars of poplar, Populus alba×berolinensis and P. ×'Zhonglin Sanbei 1', during autumn temperature drop for analyzing the roles of phenolic secondary metabolites in cold resistance. Results show that the contents of condensed tannin and flavonoid in poplar leaf and the flavonoid contents in bark of P. ×'Zhonglin Sanbei 1' were increased with the decrease of autumn minimum temperature, showing a significantly negative correlation between the contents of soluble phenolic substance in the leaves and changeable temperature. In contrast, lignin content in the poplar leaves is decreased in the process of temperature drop, showing a significantly positive correlation. These results indicate that the variation in phenolic substance has a close correlation with its cold-resistance during the autumn temperature drop. © 2011 Northeast Forestry University and Springer-Verlag Berlin Heidelberg.

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