Forest Research Institute FRI

Dehradun, India

Forest Research Institute FRI

Dehradun, India
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Damos P.,Aristotle University of Thessaloniki | Bonsignore C.P.,University of Reggio Calabria | Gardi F.,University of Reggio Calabria | Avtzis D.N.,Forest Research Institute Fri
Journal of Applied Entomology | Year: 2014

A unique logistic model for predicting population dynamics of Anarsia lineatella and Grapholita molesta was evaluated on populations sampled from Italy and Greece. Intraspecific mtDNA divergence was additionally estimated in an effort to examine whether regional differences in moth phenologies are associated with genetic divergence. A. lineatella populations displayed closer similarities on phenological responses between the two observation regions. As a result, population fluctuations in both regions could be accurately predicted based on the constructed model. However, that was not the case for G. molesta as its populations exhibited a more regional behaviour, and thus, the model was less accurate. It is notable that degree-day accumulations above the lower temperature thresholds were recorded in Italy on month earlier than in Greece (1st March in Calabria in contrast to 1st April in Veria). That kind of observed deviations in moth phenologies could be potentially attributed to regional environmental conditions or even genetic differentiation. Despite the low number of individuals analysed, this first attempt to study the levels of intraspecific divergence between Italian and Greek moth populations revealed that both species exhibit evidence of regional-based separation. Our study provides the first comprehensive phenological comparison between populations of A. lineatella and G. molesta from Italy and Greece. At the same time, the population genetic structure data reveal differentiation between these two regions for both species, something that should be further investigated as it could provide a possible explanation for the observed phenological differences. Moreover, DNA barcoding confirmed that G. molesta pheromone blends attracted at least two morphologically close-related tortricid moth species. This fact probably explains the phenological variations observed for this species as well as the difficulties in defining the number of non-overlapping flights. © 2014 Blackwell Verlag GmbH.

Tripathi N.,P.A. College | Kumar S.,P.A. College | Singh R.,P.A. College | Singh C.J.,Ministry of Environment and Forest | And 2 more authors.
Oriental Journal of Chemistry | Year: 2013

Girardinia heterophylla (Family: Urticaceae) roots has not been studied so far. The swollen base of roots were collected from and extracted with petroleum ether. The dried petroleum extract was subjected to column chromatography and TLC. Three compounds were isolated from the roots of Girardinia heferophylla. On the basis of spectral analysis they were identified as -sitosterol, y-sitosterol and ursolic acid. In this study the presence of y-sitosterol and Ursolic acid in roots of Girardinia heterophylla has been reported for the first time.

Rawat J.M.,Forest Research Institute FRI | Rawat J.M.,Parnkuti Anantpur University Road | Rawat J.M.,Central Institute of Medicinal and Aromatic Plants | Rawat B.,Gb Pant Institute Of Himalayan Environment And Development | And 2 more authors.
Biotechnology Letters | Year: 2013

Among five hairy root lines of Picrorhiza kurrooa that were established through Agrobacterium rhizogenes, one (H7) was selected for encapsulation due to high accumulation of picrotin and picrotoxinin (8.3 and 47.6 μg/g DW, respectively). Re-grown encapsulated roots induced adventitious shoots with 73 % frequency on MS medium supplemented with 0.1 μM 6-benzylaminopurine, following 6 months of storage at 25 °C. Regenerated plantlets had 85 % survival after 2 months. Regenerants were of similar morphotype having increased leaf number and branched root system as compared to non-transformed plants. The transformed nature of the plants was confirmed through PCR and Southern blot analysis. Genetic fidelity analysis of transformed plants using RAPD and ISSR showed 5.2 and 3.6 % polymorphism, respectively. Phytochemical analysis also showed that picrotin and picrotoxinin content were similar in hairy root line and its regenerants. © 2013 Springer Science+Business Media Dordrecht.

Kaur A.,Forest Research Institute FRI | Chaukiyal S.P.,Forest Research Institute FRI | Thakur A.,Forest Research Institute FRI | Pokhriyal T.C.,Forest Research Institute FRI
Journal of Forestry Research | Year: 2013

Rhizobia were isolated from Albizia lebbek (L) Benth. seedlings collected from six different places, tested against the nodulation test and inoculated into 45 day old Albizia lebbek seedlings in sterile soil mixture under glass house conditions. After a period of two and three months, the plant samples were taken to study the influence of inoculation treatments on nitrogen fixation, assimilation, biomass production and nitrogen content in different plant parts. The seedlings inoculated with isolates III(1) and III(2) of Lalpani had the maximum nodular biomass, specific (13.73 and 13.59 μ mole C2H2 reduced h-1, respectively) and total nitrogenase (11.80 and 11.16 μ mole C2H2 reduced h-1, respectively) activities in their nodules statistically at par with each other. These also exhibited high nitrate reductase activity in different plant parts. The seedlings inoculated with slow growing isolates viz. I(2) and IV(1) and the control were amongst poor performers for biomass production, nitrogenase activity and nitrate reductase activity in different plant parts. The minimum nitrogenase (specific and total) activities and low nitrogen content (%) in leaves, stems and roots were estimated in seedlings inoculated with isolate II(4) of Barkot. Nodular biomass was recorded as an indicator of nitrogen fixation activity rather than the number of nodules per plant. The isolates III(1) and III(2) can be utilized to enhance productivity in afforestation and reforestation programmes. © 2013 Northeast Forestry University and Springer-Verlag Berlin Heidelberg.

Wani A.A.,Sher-e-Kashmir University of Agricultural Sciences and Technology | Joshi P.K.,Jawaharlal Nehru University | Singh O.,Forest Research Institute FRI | Shafi S.,Sher-e-Kashmir University of Agricultural Sciences and Technology
Journal of Mountain Science | Year: 2016

The role of forests is being actively considered under the agenda of REDD+ (Reducing Emissions from Deforestation and Forest Degradation plus) aimed at reducing emissions related to changes in forest cover and forest quality. Forests in general have undergone negative changes in the past in the form of deforestation and degradation, while in some countries positive changes are reported in the form of conservation, sustainable management of forests and enhancement of carbon stock. The present study in the Kashmir Himalayan forests is an effort to assess historical forest cover changes that took place from 1980 to 2009 and to predict the same for 2030 on the basis of past trend using geospatial modeling approach. Landsat data (Multispectral Scanner (MSS), Thematic Mapper (TM) and Enhanced Thematic Mapper (ETM+)) was used for the years 1980, 1990 and (2001, 2009) respectively and change detection analysis between the dates was performed. The maps generated were validated through ground truthing. The study area (3375.62 km2) from 1980-2009 has uffered deforestation and forest degradation of about 126 km2 and 139.02 km2 respectively which can be claimed under negative options of REDD+, while as the area that experienced no change (1514 km2) can be claimed under conservation. A small area (23.31 km2) observed as positive change can be claimed under positive options. The projected estimates of forest cover for 2030 showed increased deforestation and forest degradation on the basis of trend analysis using Cellular Automata (CA) Markov modeling. Despite the fact that country as a whole has registered a net positive change in the past few decades, but there are regions like Kashmir region of western Himalaya which have constantly undergoing deforestation as well as degradation in the past few decades. © 2016, Science Press, Institute of Mountain Hazards and Environment, CAS and Springer-Verlag Berlin Heidelberg.

Tripathi N.,P.A. College | Kumar S.,P.A. College | Singh R.,P.A. College | Singh C.J.,Ministry of Environment and Forest | And 2 more authors.
Oriental Journal of Chemistry | Year: 2013

The unknown compound of code number (GHRPTB) was isolated from roots of Girardinia heterophylla. After being collected and analyzed by GC-MS and compared with NIST standard chart library, it was declared to be γ-sitosterol, an epimer of β-sitosterol.

Tanaka N.,National Museum of Nature and Science | Sugawara T.,Tokyo Metroplitan University | Aung M.M.,Forest Research Institute FRI | Murata J.,University of Tokyo
Phytotaxa | Year: 2015

A new species, Impatiens kingdon-wardii Nob. Tanaka & T. Sugaw. (Balsaminaceae), is described and illustrated from Mt. Victoria (Natma Taung), northwestern Myanmar. This species is distinguished from any other species by the pink orbicular, densely pubescent lateral sepals, appearance of which is like two ears of the mouse, and upper lobes of lateral united petals with hairy club-shaped protuberance. © 2015 Magnolia Press.

Tripathi N.,P.A. College | Singh C.J.,Ministry of Environment and Forest | Khan L.H.,Forest Research Institute FRI | Kumar S.,P.A. College | And 3 more authors.
Open Nutraceuticals Journal | Year: 2014

Girardinia heterophylla (Decne), distributed extensively in Middle Himalayas, is a potential source of Leaf Protein Concentrate (LPC). Present study envisages the phytochemical investigations to assess the availability and commercial viability of LPC of G. heterophylla. Samples of plant leaves were collected from Mussoorie Hills of Uttarakhand (India) and contents of LPC were isolated. Proximate analysis of LPC for nitrogen, protein, fat, ash and carbohydrate content revealed their concentration in appreciable quantities making it a potential source of non-conventional protein. LPC has important biochemical characteristics, which establish the usefulness and potential of leaves of this plant to be used as animal fodder for better milk production and also as nutritionally rich source for protein for human consumption. © Tripathi et al.

Wani A.A.,Forest Research Institute FRI | Wani A.A.,Sher-e-Kashmir University of Agricultural Sciences and Technology | Joshi P.K.,TERI University | Singh O.,Forest Research Institute FRI | Bhat J.A.,Sher-e-Kashmir University of Agricultural Sciences and Technology
Mitigation and Adaptation Strategies for Global Change | Year: 2014

Soil physical and chemical properties were quantified to assess soil organic carbon (SOC) density (t ha-1) and SOC CO2 mitigation (t ha-1) under six forest strata Cedrus deodara (closed) (S1), Cedrus deodara (open) (S2), Abies pindrow-Picea smithiana (closed) (S3), Abies pindrow-Picea smithiana (open) (S4), Pinus wallichiana (closed) (S5) and Pinus wallichiana (open) (S6) in the southern region of Kashmir Himalayas India. Lowest average bulk density (Db) of 0.95 was found same in S3 (σ ± 0.07) and S5 (σ ± 0.09) and highest Db (1.08) was observed in S2 (σ ± 0.05). A relatively higher coarse fraction was observed in all the six strata ranging from 19.23 (SD ± 4.66) in S3 to 29.37 (σ ± 6.12) in S6. Soil pH ranged from 6.09 (σ ± 0.64) in S4 to 6.97 (σ ± 0.53) in S2. The region under biotic interference has observed significant deforestation and degradation in the past two decades leading to lower SOC% values compared to other studies in the adjoining regions of Indian Himalayas and temperate coniferous forests in general. SOC% values were observed to range from 1.03 (σ ± 0.22) in S2 to 2.25 (σ ± 0.23) in S3. SOC density ranged between 25.11 (σ ± 5.41) t ha-1 in S2 and 51.93 (σ ± 5.24) t ha-1 in S3. SOC CO2 mitigation density was found highest 190.59 (σ ± 19.23) t ha-1 in S3 and lowest 92.16 (σ ± 19.86) t ha-1 in S2. A significant variation was observed in SOC density within strata. SOC density values in closed strata in general exceed to those in open strata. Primary results indicate that the average SOC stock for all the strata is low due to continuous biotic pressure in the last two decades making it a potential region for SOC buildup under plus options of REDD + (Reducing emissions from deforestation and forest degradation) which includes conservation, sustainable management of forests and enhancement of forest carbon (C) stocks. © 2013, Springer Science+Business Media Dordrecht.

Tripathi N.,P.A. College | Kumar S.,P.A. College | Singh R.,P.A. College | Singh C.J.,Ministry of Environment and Forest | And 2 more authors.
Current Nutrition and Food Science | Year: 2014

The Leaf Protein Concentrate (LPC) was isolated from the leaves of Girardinia heterophylla found in the Mussoorie hills of Uttarakhand (India). Results of proximate analysis for nitrogen, protein, fat, ash and carbohydrate in appreciable quantities revealed its possible use as non-conventional protein source. LPC is an important characteristic, which establishes the usefulness of leaves of this plant as animal fodder for better milk production and also as nutritionally rich source of protein for human consumption. © 2014 Bentham Science Publishers.

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