Oeiras, Portugal
Oeiras, Portugal

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Canales J.,University of Malaga | Bautista R.,Plataforma Andaluza de Bioinformatica | Label P.,University Blaise Pascal | Gomez-Maldonado J.,University of Malaga | And 34 more authors.
Plant Biotechnology Journal | Year: 2014

Maritime pine (Pinus pinasterAit.) is a widely distributed conifer species in Southwestern Europe and one of the most advanced models for conifer research. In the current work, comprehensive characterization of the maritime pine transcriptome was performed using a combination of two different next-generation sequencing platforms, 454 and Illumina. De novo assembly of the transcriptome provided a catalogue of 26 020 unique transcripts in maritime pine trees and a collection of 9641 full-length cDNAs. Quality of the transcriptome assembly was validated by RT-PCR amplification of selected transcripts for structural and regulatory genes. Transcription factors and enzyme-encoding transcripts were annotated. Furthermore, the available sequencing data permitted the identification of polymorphisms and the establishment of robust single nucleotide polymorphism (SNP) and simple-sequence repeat (SSR) databases for genotyping applications and integration of translational genomics in maritime pine breeding programmes. All our data are freely available at SustainpineDB, the P. pinaster expressional database. Results reported here on the maritime pine transcriptome represent a valuable resource for future basic and applied studies on this ecological and economically important pine species. © 2013 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.

Pintos B.,Forest Biotech Laboratory | Manzanera J.A.,Technical University of Madrid | Bueno M.A.,Forest Biotech Laboratory
Annals of Forest Science | Year: 2010

Development of both somatic and gametic embryogenesis has many applications in clonal forestry and genetic improvement, for instance as mass-propagation of genetically improved plants and production of pure lines through doubled-haploid plant regeneration from gametic embryos. The goal of this work was to improve growth, maturation and plantlet regeneration of cork oak (Quercus suber L.) embryos from both somatic and gametic origin. Activated charcoal promoted a significant increase in growth in terms of relative size and weight of both somatic and doubled-haploid embryos, as well as a more efficient control of secondary somatic embryogenesis during development. A significant interaction was also observed with amino acid nutrition. While some amino acids (i.e., glutamine, arginine or asparagine) did not show significant differences with the controls, a mixture of these three amino acids or gamma amino butyric acid stimulated embryo growth. The highest survival rate during acclimation of plantlets from both somatic and doubled-haploid origin was obtained when the embryos had been previously cultured on basal medium with 3% sucrose and 1% activated charcoal for two months at 4 °C and germinated on medium supplemented with 6-benzylaminopurine and indole-3-butyric acid. We obtained more than 900 cork oak plantlets acclimated from several embryogenic lines, with a high survival rate, demonstrating that this methodology is applicable for large scale plantlet production. We also report the first regeneration of doubled-haploid plantlets in cork oak. © INRA, EDP Sciences, 2010.

Mohamed R.,Forest Biotech Laboratory | Wong M.T.,Forest Biotech Laboratory | Halis R.,University Putra Malaysia
Pertanika Journal of Tropical Agricultural Science | Year: 2013

Aquilaria produces fragrant wood known as 'gaharu' in its stem and branches, often in mature and damaged trees. In this study, anatomical characteristics in juvenile and mature trees were investigated by comparing their anatomical structures after various staining methods and direct observations under a light microscope. Juvenile and mature wood share similar anatomical structures. No major differences were observed other than the percentage of area covered by included phloem in juvenile was 2.16 times more than that of the mature wood. Microscopic observation revealed that in mature resinous wood, brownish bodies were found in ray and axial parenchyma, included phloem, xylem vessels and fibres, and this finding indicates that these are important elements for 'gaharu' depositing. Thus, it was concluded that juvenile tree possess the anatomical features of that of mature wood in producing 'gaharu'. © Universiti Putra Malaysia Press.

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